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Protein

Prostasin

Gene

Prss8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Possesses a trypsin-like cleavage specificity with a preference for poly-basic substrates (By similarity). Stimulates epithelial sodium channel (ENaC) activity through activating cleavage of the gamma subunits (SCNN1G).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei85Charge relay systemBy similarity1
Active sitei134Charge relay systemBy similarity1
Active sitei238Charge relay systemBy similarity1

GO - Molecular functioni

  • serine-type endopeptidase activity Source: InterPro
  • sodium channel regulator activity Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease

Protein family/group databases

MEROPSiS01.159

Names & Taxonomyi

Protein namesi
Recommended name:
Prostasin (EC:3.4.21.-)
Alternative name(s):
Channel-activating protease 1
Short name:
CAP1
Serine protease 8
Cleaved into the following 2 chains:
Gene namesi
Name:Prss8
Synonyms:Cap1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:1923810 Prss8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei320 – 340HelicalSequence analysisAdd BLAST21

Keywords - Cellular componenti

Cell membrane, Membrane, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
PropeptideiPRO_000002803130 – 32Activation peptideBy similarity3
ChainiPRO_000024051233 – 322ProstasinAdd BLAST290
ChainiPRO_000002803233 – 44Prostasin light chainAdd BLAST12
ChainiPRO_000002803345 – 322Prostasin heavy chainAdd BLAST278
PropeptideiPRO_0000028034323 – 342By similarityAdd BLAST20

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi37 ↔ 154Interchain (between light and heavy chains)PROSITE-ProRule annotation
Disulfide bondi70 ↔ 86PROSITE-ProRule annotation
Glycosylationi110N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi159N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi168 ↔ 244PROSITE-ProRule annotation
Disulfide bondi201 ↔ 223PROSITE-ProRule annotation
Disulfide bondi234 ↔ 262PROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

MaxQBiQ9ESD1
PaxDbiQ9ESD1
PRIDEiQ9ESD1

Expressioni

Gene expression databases

CleanExiMM_PRSS8

Interactioni

Subunit structurei

Heterodimer of two chains, light and heavy, held by a disulfide bond.By similarity

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032988

Structurei

3D structure databases

ProteinModelPortaliQ9ESD1
SMRiQ9ESD1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini45 – 286Peptidase S1PROSITE-ProRule annotationAdd BLAST242

Sequence similaritiesi

Belongs to the peptidase S1 family.PROSITE-ProRule annotation

Keywords - Domaini

Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9ESD1
PhylomeDBiQ9ESD1

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9ESD1-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPRVGLGLG QLEAVTILLL LGLLQSGIRA DGTEASCGAV IQPRITGGGS
60 70 80 90 100
AKPGQWPWQV SITYDGNHVC GGSLVSNKWV VSAAHCFPRE HSREAYEVKL
110 120 130 140 150
GAHQLDSYSN DTVVHTVAQI ITHSSYREEG SQGDIAFIRL SSPVTFSRYI
160 170 180 190 200
RPICLPAANA SFPNGLHCTV TGWGHVAPSV SLQTPRPLQQ LEVPLISRET
210 220 230 240 250
CSCLYNINAV PEEPHTIQQD MLCAGYVKGG KDACQGDSGG PLSCPMEGIW
260 270 280 290 300
YLAGIVSWGD ACGAPNRPGV YTLTSTYASW IHHHVAELQP RVVPQTQESQ
310 320 330 340
PDGHLCNHHP VFSSAAAPKL LRPVLFLPLG LTLGLLSLWL EH
Length:342
Mass (Da):36,729
Last modified:April 27, 2001 - v2
Checksum:i0620DE88ED187D0F
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q99L44Q99L44_MOUSE
Prostasin
Prss8
339Annotation score:
A0A0U1RQA6A0A0U1RQA6_MOUSE
Prostasin
Prss8
62Annotation score:

Sequence cautioni

The sequence AAG17054 differs from that shown. Reason: Frameshift at position 339.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188613 mRNA Translation: AAG17054.1 Frameshift.
UniGeneiMm.5875

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF188613 mRNA Translation: AAG17054.1 Frameshift.
UniGeneiMm.5875

3D structure databases

ProteinModelPortaliQ9ESD1
SMRiQ9ESD1
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000032988

Protein family/group databases

MEROPSiS01.159

Proteomic databases

MaxQBiQ9ESD1
PaxDbiQ9ESD1
PRIDEiQ9ESD1

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:1923810 Prss8

Phylogenomic databases

eggNOGiKOG3627 Eukaryota
COG5640 LUCA
HOGENOMiHOG000251820
HOVERGENiHBG013304
InParanoidiQ9ESD1
PhylomeDBiQ9ESD1

Miscellaneous databases

PROiPR:Q9ESD1
SOURCEiSearch...

Gene expression databases

CleanExiMM_PRSS8

Family and domain databases

CDDicd00190 Tryp_SPc, 1 hit
InterProiView protein in InterPro
IPR009003 Peptidase_S1_PA
IPR001314 Peptidase_S1A
IPR001254 Trypsin_dom
IPR018114 TRYPSIN_HIS
IPR033116 TRYPSIN_SER
PfamiView protein in Pfam
PF00089 Trypsin, 1 hit
PRINTSiPR00722 CHYMOTRYPSIN
SMARTiView protein in SMART
SM00020 Tryp_SPc, 1 hit
SUPFAMiSSF50494 SSF50494, 1 hit
PROSITEiView protein in PROSITE
PS50240 TRYPSIN_DOM, 1 hit
PS00134 TRYPSIN_HIS, 1 hit
PS00135 TRYPSIN_SER, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPRSS8_MOUSE
AccessioniPrimary (citable) accession number: Q9ESD1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 27, 2001
Last sequence update: April 27, 2001
Last modified: November 7, 2018
This is version 124 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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