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Protein

Exostosin-like 2

Gene

Extl2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Glycosyltransferase required for the biosynthesis of heparan-sulfate and responsible for the alternating addition of beta-1-4-linked glucuronic acid (GlcA) and alpha-1-4-linked N-acetylglucosamine (GlcNAc) units to nascent heparan sulfate chains.By similarity

Catalytic activityi

UDP-N-acetyl-alpha-D-glucosamine + [protein]-3-O-(beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine = UDP + [protein]-3-O-(alpha-D-GlcNAc-(1->4)-beta-D-GlcA-(1->3)-beta-D-Gal-(1->3)-beta-D-Gal-(1->4)-beta-D-Xyl)-L-serine.1 Publication

Cofactori

Mn2+1 Publication

Kineticsi

  1. KM=320 µM for UDP-GalNAc1 Publication
  2. KM=560 µM for UDP-GlcNAc1 Publication

    Sites

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Binding sitei101Substrate1 Publication1
    Metal bindingi153Manganese; catalytic1 Publication1
    Binding sitei181Substrate1 Publication1
    Active sitei2461 Publication1

    GO - Molecular functioni

    GO - Biological processi

    Keywordsi

    Molecular functionGlycosyltransferase, Transferase
    LigandManganese, Metal-binding

    Protein family/group databases

    CAZyiGT64 Glycosyltransferase Family 64

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Exostosin-like 2 (EC:2.4.1.2231 Publication)
    Alternative name(s):
    Alpha-1,4-N-acetylhexosaminyltransferase EXTL2
    Alpha-GalNAcT EXTL2
    EXT-related protein 2
    Glucuronyl-galactosyl-proteoglycan 4-alpha-N-acetylglucosaminyltransferase
    Gene namesi
    Name:Extl2
    Synonyms:Extr2
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
    Proteomesi
    • UP000000589 Componenti: Chromosome 3

    Organism-specific databases

    MGIiMGI:1889574 Extl2

    Subcellular locationi

    Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

    Topology

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Topological domaini1 – 21CytoplasmicSequence analysisAdd BLAST21
    Transmembranei22 – 42Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
    Topological domaini43 – 330LumenalSequence analysisAdd BLAST288

    Keywords - Cellular componenti

    Endoplasmic reticulum, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Mutagenesisi243N → A: Reduced activity. 1 Publication1
    Mutagenesisi246D → A: Abolishes enzyme activity. 1 Publication1
    Mutagenesisi293R → A: Reduced activity. 1 Publication1

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    ChainiPRO_00001496561 – 330Exostosin-like 2Add BLAST330

    Amino acid modifications

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Glycosylationi75N-linked (GlcNAc...) asparagineSequence analysis1
    Disulfide bondi244 ↔ 2961 Publication

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiQ9ES89
    PaxDbiQ9ES89
    PRIDEiQ9ES89

    PTM databases

    PhosphoSitePlusiQ9ES89

    Expressioni

    Gene expression databases

    BgeeiENSMUSG00000027963 Expressed in 264 organ(s), highest expression level in substantia nigra
    CleanExiMM_EXTL2
    ExpressionAtlasiQ9ES89 baseline and differential
    GenevisibleiQ9ES89 MM

    Interactioni

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575

    Structurei

    Secondary structure

    1330
    Legend: HelixTurnBeta strandPDB Structure known for this area
    Show more details

    3D structure databases

    DisProtiDP00397
    ProteinModelPortaliQ9ES89
    SMRiQ9ES89
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiQ9ES89

    Family & Domainsi

    Region

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Regioni130 – 135Substrate binding1 Publication6
    Regioni151 – 153Substrate binding1 Publication3
    Regioni242 – 246Substrate binding1 Publication5
    Regioni280 – 293Substrate binding1 PublicationAdd BLAST14

    Sequence similaritiesi

    Belongs to the glycosyltransferase 47 family.Curated

    Keywords - Domaini

    Signal-anchor, Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiKOG1022 Eukaryota
    ENOG410XQYV LUCA
    GeneTreeiENSGT00550000074496
    HOGENOMiHOG000054215
    HOVERGENiHBG051525
    InParanoidiQ9ES89
    KOiK02369
    OrthoDBiEOG091G0A1H
    PhylomeDBiQ9ES89
    TreeFamiTF314231

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR004263 Exostosin
    IPR015338 EXT_C
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR11062 PTHR11062, 2 hits
    PfamiView protein in Pfam
    PF09258 Glyco_transf_64, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit

    Sequence (1+)i

    Sequence statusi: Complete.

    This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

    Q9ES89-1 [UniParc]FASTAAdd to basket
    « Hide
            10         20         30         40         50
    MMRGCHICKL PGRVMGIRVL RFSLVVILVL LLVAGALTNL LPNIKEDKML
    60 70 80 90 100
    TLRREIKSPS KSALDSFTLI MQTYNRTDLL LRLLNHYQAV PSLHKVIVVW
    110 120 130 140 150
    NNVGEKGPEE LWNSLGPHPI PVIFKPQTAN KMRNRLQVFP EVETNAVLMV
    160 170 180 190 200
    DDDTLISAQD LVFAFSIWQQ FPDQIIGFVP RKHVSTSSGI YSYGGFELQT
    210 220 230 240 250
    PGPGNGDQYS MVLIGASFFN SKYLELFQKQ PAAVHALIDE TQNCDDIAMN
    260 270 280 290 300
    FLVTRHTGKP SGIFVKPINM VNLEKETNGY SGMWHRAEHF LQRSYCINKL
    310 320 330
    VNIYDGMPLK YSNIMISQFG FPYANHKSKM
    Length:330
    Mass (Da):37,391
    Last modified:March 1, 2001 - v1
    Checksum:i51F88BE5D3E5EADF
    GO

    Computationally mapped potential isoform sequencesi

    There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
    EntryEntry nameProtein names
    Gene namesLengthAnnotation
    Q3TSR0Q3TSR0_MOUSE
    Exostosin-like 2
    Extl2
    316Annotation score:

    Experimental Info

    Feature keyPosition(s)DescriptionActionsGraphical viewLength
    Sequence conflicti191Y → D in BAB31683 (PubMed:16141072).Curated1

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA Translation: AAG17542.1
    AK019370 mRNA Translation: BAB31683.2
    BC031438 mRNA Translation: AAH31438.1
    BC094444 mRNA Translation: AAH94444.1
    CCDSiCCDS17784.1
    RefSeqiNP_001156986.1, NM_001163514.1
    NP_067363.3, NM_021388.4
    UniGeneiMm.41739

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963
    GeneIDi58193
    KEGGimmu:58193
    UCSCiuc008rbu.2 mouse

    Similar proteinsi

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBLi
    GenBanki
    DDBJi
    Links Updated
    AF200973 mRNA Translation: AAG17542.1
    AK019370 mRNA Translation: BAB31683.2
    BC031438 mRNA Translation: AAH31438.1
    BC094444 mRNA Translation: AAH94444.1
    CCDSiCCDS17784.1
    RefSeqiNP_001156986.1, NM_001163514.1
    NP_067363.3, NM_021388.4
    UniGeneiMm.41739

    3D structure databases

    Select the link destinations:
    PDBei
    RCSB PDBi
    PDBji
    Links Updated
    PDB entryMethodResolution (Å)ChainPositionsPDBsum
    1OMXX-ray2.40A/B38-330[»]
    1OMZX-ray2.10A/B38-330[»]
    1ON6X-ray2.30A/B38-330[»]
    1ON8X-ray2.70A/B38-330[»]
    DisProtiDP00397
    ProteinModelPortaliQ9ES89
    SMRiQ9ES89
    ModBaseiSearch...
    MobiDBiSearch...

    Protein-protein interaction databases

    STRINGi10090.ENSMUSP00000029575

    Protein family/group databases

    CAZyiGT64 Glycosyltransferase Family 64

    PTM databases

    PhosphoSitePlusiQ9ES89

    Proteomic databases

    MaxQBiQ9ES89
    PaxDbiQ9ES89
    PRIDEiQ9ES89

    Protocols and materials databases

    Structural Biology KnowledgebaseSearch...

    Genome annotation databases

    EnsembliENSMUST00000029575; ENSMUSP00000029575; ENSMUSG00000027963
    ENSMUST00000106502; ENSMUSP00000102111; ENSMUSG00000027963
    GeneIDi58193
    KEGGimmu:58193
    UCSCiuc008rbu.2 mouse

    Organism-specific databases

    CTDi2135
    MGIiMGI:1889574 Extl2

    Phylogenomic databases

    eggNOGiKOG1022 Eukaryota
    ENOG410XQYV LUCA
    GeneTreeiENSGT00550000074496
    HOGENOMiHOG000054215
    HOVERGENiHBG051525
    InParanoidiQ9ES89
    KOiK02369
    OrthoDBiEOG091G0A1H
    PhylomeDBiQ9ES89
    TreeFamiTF314231

    Miscellaneous databases

    ChiTaRSiExtl2 mouse
    EvolutionaryTraceiQ9ES89
    PROiPR:Q9ES89
    SOURCEiSearch...

    Gene expression databases

    BgeeiENSMUSG00000027963 Expressed in 264 organ(s), highest expression level in substantia nigra
    CleanExiMM_EXTL2
    ExpressionAtlasiQ9ES89 baseline and differential
    GenevisibleiQ9ES89 MM

    Family and domain databases

    Gene3Di3.90.550.10, 1 hit
    InterProiView protein in InterPro
    IPR004263 Exostosin
    IPR015338 EXT_C
    IPR029044 Nucleotide-diphossugar_trans
    PANTHERiPTHR11062 PTHR11062, 2 hits
    PfamiView protein in Pfam
    PF09258 Glyco_transf_64, 1 hit
    SUPFAMiSSF53448 SSF53448, 1 hit
    ProtoNetiSearch...

    Entry informationi

    Entry nameiEXTL2_MOUSE
    AccessioniPrimary (citable) accession number: Q9ES89
    Secondary accession number(s): Q505Q8, Q9CX90
    Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 27, 2002
    Last sequence update: March 1, 2001
    Last modified: November 7, 2018
    This is version 132 of the entry and version 1 of the sequence. See complete history.
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families
    2. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    UniProt is an ELIXIR core data resource
    Main funding by: National Institutes of Health

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