UniProtKB - Q9ES71 (GNPAT_RAT)
Protein
Dihydroxyacetone phosphate acyltransferase
Gene
Gnpat
Organism
Rattus norvegicus (Rat)
Status
Functioni
Dihydroxyacetonephosphate acyltransferase involved in plasmalogen biosynthesis.By similarity
Catalytic activityi
- EC:2.3.1.42
- dihydroxyacetone phosphate + hexadecanoyl-CoA = 1-hexadecanoylglycerone 3-phosphate + CoABy similarityThis reaction proceeds in the forwardBy similarity direction.
: glycerophospholipid metabolism Pathwayi
This protein is involved in the pathway glycerophospholipid metabolism, which is part of Membrane lipid metabolism.View all proteins of this organism that are known to be involved in the pathway glycerophospholipid metabolism and in Membrane lipid metabolism.
GO - Molecular functioni
- catalytic activity Source: RGD
- glycerone-phosphate O-acyltransferase activity Source: RGD
- palmitoyl-CoA hydrolase activity Source: RGD
GO - Biological processi
- cerebellum morphogenesis Source: RGD
- ether lipid biosynthetic process Source: RGD
- fatty acid metabolic process Source: GO_Central
- glycerophospholipid metabolic process Source: UniProtKB-UniPathway
- membrane organization Source: RGD
- myelination Source: RGD
- paranodal junction assembly Source: RGD
- phospholipid biosynthetic process Source: GO_Central
- response to drug Source: RGD
- response to fatty acid Source: RGD
- response to nutrient Source: RGD
- response to starvation Source: RGD
- synapse assembly Source: RGD
Keywordsi
Molecular function | Acyltransferase, Transferase |
Enzyme and pathway databases
BRENDAi | 2.3.1.42, 5301 |
Reactomei | R-RNO-1483166, Synthesis of PA R-RNO-75896, Plasmalogen biosynthesis R-RNO-9033241, Peroxisomal protein import |
SABIO-RKi | Q9ES71 |
UniPathwayi | UPA00940 |
Names & Taxonomyi
Protein namesi | Recommended name: Dihydroxyacetone phosphate acyltransferaseCurated (EC:2.3.1.42)Short name: DAP-AT Short name: DHAP-AT Alternative name(s): Acyl-CoA:dihydroxyacetonephosphateacyltransferase Glycerone-phosphate O-acyltransferase |
Gene namesi | |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620179, Gnpat |
Subcellular locationi
Peroxisome
- Peroxisome membrane 1 Publication; Peripheral membrane protein 1 Publication; Matrix side 1 Publication
Note: Exclusively localized to the lumenal side of the peroxisomal membrane.By similarity1 Publication
Mitochondrion
- mitochondrial membrane Source: GO_Central
- mitochondrion Source: RGD
Peroxisome
- peroxisomal membrane Source: UniProtKB
- peroxisome Source: HGNC-UCL
Keywords - Cellular componenti
Membrane, PeroxisomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000195248 | 1 – 678 | Dihydroxyacetone phosphate acyltransferaseAdd BLAST | 678 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 12 | PhosphoserineCombined sources | 1 | |
Modified residuei | 17 | PhosphoserineCombined sources | 1 | |
Modified residuei | 641 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9ES71 |
PRIDEi | Q9ES71 |
PTM databases
iPTMneti | Q9ES71 |
PhosphoSitePlusi | Q9ES71 |
Expressioni
Tissue specificityi
Expressed in liver (at protein level).1 Publication
Gene expression databases
Bgeei | ENSRNOG00000019205, Expressed in testis and 21 other tissues |
Genevisiblei | Q9ES71, RN |
Interactioni
Subunit structurei
May be part of a heterotrimeric complex composed of DAP-AT, ADAP-S and a modified form of DAP-AT.
By similarityProtein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026617 |
Family & Domainsi
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 161 – 166 | HXXXXD motif | 6 | |
Motifi | 676 – 678 | Microbody targeting signalSequence analysis | 3 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 5 – 9 | Poly-Ser | 5 |
Domaini
The HXXXXD motif is essential for acyltransferase activity and may constitute the binding site for the phosphate moiety of the glycerol-3-phosphate.By similarity
Sequence similaritiesi
Belongs to the GPAT/DAPAT family.Curated
Phylogenomic databases
eggNOGi | KOG3730, Eukaryota |
GeneTreei | ENSGT00520000055570 |
HOGENOMi | CLU_017332_0_1_1 |
InParanoidi | Q9ES71 |
OMAi | MLKNGFA |
OrthoDBi | 198209at2759 |
PhylomeDBi | Q9ES71 |
TreeFami | TF313360 |
Family and domain databases
CDDi | cd07993, LPLAT_DHAPAT-like, 1 hit |
InterProi | View protein in InterPro IPR028353, DHAPAT IPR022284, GPAT/DHAPAT IPR041728, GPAT/DHAPAT_LPLAT IPR002123, Plipid/glycerol_acylTrfase |
PANTHERi | PTHR12563, PTHR12563, 1 hit |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
PIRSFi | PIRSF500063, DHAPAT, 1 hit PIRSF000437, GPAT_DHAPAT, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9ES71-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDVPSSSSSR FSVGSASPSS VLLYAKDLKK WDEFEDLLEE RRQVSDFKFA
60 70 80 90 100
MKCYTPPLYR GITPCKPSDI KSIVLNSEEI NYVIKQLSRE SLTGVDVLRE
110 120 130 140 150
EANEILEEMS HKLRIGAIRF FAFVLSKVFK QIFSKVCVNE EGIQKLQRAI
160 170 180 190 200
QEHPVILLPS HRSYIDFLML SFVLYNYDLP VPVIAAGMDF LGMRVVSELL
210 220 230 240 250
RMSGAFFMRR TFGGNKLYWA VFSEYVKTML RSGYAPVEFF LEGTRSRAAK
260 270 280 290 300
TLTPKFGLLN IVMEPFFKRE VFDTYFVPIS ISYDKILEES LYAYELLGIP
310 320 330 340 350
KPKESTTGLL KARRILSENF GSIHVYFGDP VSLRSLAAGR LSRNTYNLVP
360 370 380 390 400
RCIPQKQPED VQAFVTEVAY KMQLLQIENL ALSPWLLVVA ILLQNQLCMD
410 420 430 440 450
FDALVEKTLW LKGLTQVFGG FLLWPDNKLP EEVVQSSILL HSNLATLVKD
460 470 480 490 500
QVVLKVDSES SQMVNGLVPQ HIAFLMCSAY RNQLLNVFAR PSLVAVALHM
510 520 530 540 550
TPGLRKEDVF SCFSFLRNVF SDEFIFLPGN TLRDFEEGCY LLCKTEVMQM
560 570 580 590 600
TGKDIILTDK GNAVLQFLTG LFKPFVESYQ ILSKCLLHEE DYFSEKEYLV
610 620 630 640 650
TARKFTRQLL DQDASQCYDA LSSELQKNAL AAFVRLGVVE KNKVDSKYVY
660 670
YVNGPATSKL EEMLGCKKPI GKPATAKL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF218826 mRNA Translation: AAG17548.1 |
RefSeqi | NP_445862.1, NM_053410.1 |
Genome annotation databases
Ensembli | ENSRNOT00000026617; ENSRNOP00000026617; ENSRNOG00000019205 |
GeneIDi | 84470 |
KEGGi | rno:84470 |
UCSCi | RGD:620179, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF218826 mRNA Translation: AAG17548.1 |
RefSeqi | NP_445862.1, NM_053410.1 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000026617 |
PTM databases
iPTMneti | Q9ES71 |
PhosphoSitePlusi | Q9ES71 |
Proteomic databases
PaxDbi | Q9ES71 |
PRIDEi | Q9ES71 |
Genome annotation databases
Ensembli | ENSRNOT00000026617; ENSRNOP00000026617; ENSRNOG00000019205 |
GeneIDi | 84470 |
KEGGi | rno:84470 |
UCSCi | RGD:620179, rat |
Organism-specific databases
CTDi | 8443 |
RGDi | 620179, Gnpat |
Phylogenomic databases
eggNOGi | KOG3730, Eukaryota |
GeneTreei | ENSGT00520000055570 |
HOGENOMi | CLU_017332_0_1_1 |
InParanoidi | Q9ES71 |
OMAi | MLKNGFA |
OrthoDBi | 198209at2759 |
PhylomeDBi | Q9ES71 |
TreeFami | TF313360 |
Enzyme and pathway databases
UniPathwayi | UPA00940 |
BRENDAi | 2.3.1.42, 5301 |
Reactomei | R-RNO-1483166, Synthesis of PA R-RNO-75896, Plasmalogen biosynthesis R-RNO-9033241, Peroxisomal protein import |
SABIO-RKi | Q9ES71 |
Miscellaneous databases
PROi | PR:Q9ES71 |
Gene expression databases
Bgeei | ENSRNOG00000019205, Expressed in testis and 21 other tissues |
Genevisiblei | Q9ES71, RN |
Family and domain databases
CDDi | cd07993, LPLAT_DHAPAT-like, 1 hit |
InterProi | View protein in InterPro IPR028353, DHAPAT IPR022284, GPAT/DHAPAT IPR041728, GPAT/DHAPAT_LPLAT IPR002123, Plipid/glycerol_acylTrfase |
PANTHERi | PTHR12563, PTHR12563, 1 hit |
Pfami | View protein in Pfam PF01553, Acyltransferase, 1 hit |
PIRSFi | PIRSF500063, DHAPAT, 1 hit PIRSF000437, GPAT_DHAPAT, 1 hit |
SMARTi | View protein in SMART SM00563, PlsC, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | GNPAT_RAT | |
Accessioni | Q9ES71Primary (citable) accession number: Q9ES71 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 1, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 125 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families