UniProtKB - Q9ES54 (NPL4_RAT)
Protein
Nuclear protein localization protein 4 homolog
Gene
Nploc4
Organism
Rattus norvegicus (Rat)
Status
Functioni
The ternary complex containing UFD1, VCP and NPLOC4 binds ubiquitinated proteins and is necessary for the export of misfolded proteins from the ER to the cytoplasm, where they are degraded by the proteasome. The NPLOC4-UFD1-VCP complex regulates spindle disassembly at the end of mitosis and is necessary for the formation of a closed nuclear envelope (PubMed:10811609, PubMed:11740563, PubMed:11781570, PubMed:12411482, PubMed:12644454, PubMed:14636562). Acts as a negative regulator of type I interferon production via the complex formed with VCP and UFD1, which binds to DDX58/RIG-I and recruits RNF125 to promote ubiquitination and degradation of DDX58/RIG-I (By similarity).By similarity6 Publications
: proteasomal ubiquitin-dependent pathway Pathwayi
This protein is involved in the pathway proteasomal ubiquitin-dependent pathway, which is part of Protein degradation.View all proteins of this organism that are known to be involved in the pathway proteasomal ubiquitin-dependent pathway and in Protein degradation.
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 580 – 608 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 29 |
GO - Molecular functioni
- ATPase binding Source: ParkinsonsUK-UCL
- metal ion binding Source: UniProtKB-KW
- protein-containing complex binding Source: RGD
- ubiquitin binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
GO - Biological processi
- Golgi organization Source: HGNC-UCL
- negative regulation of RIG-I signaling pathway Source: UniProtKB
- negative regulation of type I interferon production Source: UniProtKB
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: UniProtKB-UniPathway
- retrograde protein transport, ER to cytosol Source: ParkinsonsUK-UCL
- ubiquitin-dependent protein catabolic process Source: UniProtKB
Keywordsi
Molecular function | Chaperone |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-110320, Translesion Synthesis by POLH |
UniPathwayi | UPA00144 |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear protein localization protein 4 homologShort name: Protein NPL4 |
Gene namesi | Name:Nploc4 Synonyms:Npl4 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620794, Nploc4 |
Subcellular locationi
Cytosol
- cytosol 1 Publication
Endoplasmic reticulum
- Endoplasmic reticulum 1 Publication
Nucleus
- Nucleus 1 Publication
Note: Associated with the endoplasmic reticulum and nuclear.
Cytosol
- cytosol Source: RGD
Endoplasmic reticulum
- VCP-NPL4-UFD1 AAA ATPase complex Source: ParkinsonsUK-UCL
Nucleus
- nucleus Source: RGD
Other locations
- UFD1-NPL4 complex Source: ParkinsonsUK-UCL
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000057943 | 2 – 608 | Nuclear protein localization protein 4 homologAdd BLAST | 607 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 179 | N6-acetyllysineBy similarity | 1 |
Keywords - PTMi
AcetylationProteomic databases
jPOSTi | Q9ES54 |
PaxDbi | Q9ES54 |
PRIDEi | Q9ES54 |
PTM databases
iPTMneti | Q9ES54 |
PhosphoSitePlusi | Q9ES54 |
Interactioni
Subunit structurei
Heterodimer with UFD1 (PubMed:10811609, PubMed:12644454). The heterodimer binds ubiquitinated proteins (PubMed:12644454). The heterodimer binds to VCP and inhibits Golgi membrane fusion (PubMed:10811609, PubMed:12644454).
Interacts with ZFAND2B; probably through VCP (By similarity).
By similarity2 PublicationsBinary interactionsi
Hide detailsQ9ES54
With | #Exp. | IntAct |
---|---|---|
Ufd1 [Q9ES53] | 6 | EBI-1993990,EBI-399031 |
Ufd1 [P70362] from Mus musculus. | 18 | EBI-1993990,EBI-7961331 |
Vcp [Q01853] from Mus musculus. | 11 | EBI-1993990,EBI-80597 |
GO - Molecular functioni
- ATPase binding Source: ParkinsonsUK-UCL
- ubiquitin binding Source: BHF-UCL
- ubiquitin protein ligase binding Source: BHF-UCL
Protein-protein interaction databases
BioGRIDi | 250817, 8 interactors |
ComplexPortali | CPX-138, Vcp-Npl4-Ufd1 AAA ATPase complex |
CORUMi | Q9ES54 |
DIPi | DIP-41365N |
IntActi | Q9ES54, 11 interactors |
MINTi | Q9ES54 |
STRINGi | 10116.ENSRNOP00000051856 |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9ES54 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9ES54 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 226 – 363 | MPNPROSITE-ProRule annotationAdd BLAST | 138 |
Domaini
Binds ubiquitinated proteins via its RanBP2-type zinc finger.1 Publication
Sequence similaritiesi
Belongs to the NPL4 family.Curated
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 580 – 608 | RanBP2-typePROSITE-ProRule annotationAdd BLAST | 29 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG2834, Eukaryota |
InParanoidi | Q9ES54 |
OrthoDBi | 1106766at2759 |
PhylomeDBi | Q9ES54 |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR024682, Npl4_Ub-like_dom IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF11543, UN_NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9ES54-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAESIIIRVQ SPDGVKRITA TKRETAATFL KKVAKEFGFQ NNGFSVYINR
60 70 80 90 100
NKTGEITASS SKSLHLLKIK HGDLLFLFPS SLAGPSSEME TSTSVGLKAF
110 120 130 140 150
GAPHVVEDEI DQYLSKQDGK IYRSRDPQLC RHGPLGKCVH CVPLEPFDED
160 170 180 190 200
YLNHLEPPVK HMSFHAYIRK LTGGADKGKF VALENISCKI KSGCEGHLPW
210 220 230 240 250
PNGICTKCQP SAITLNRQKY RHVDNIMFEN HTVADRFLDF WRKTGNQHFG
260 270 280 290 300
YLYGRYTEHK DIPLGIRAEV AAIYEPPQIG TQNSLELLED PKAEVVDEIA
310 320 330 340 350
SKLGLRKVGW IFTDLVSEDT RKGTVRYSRN KDTYFLSSEE CITAGDFQNK
360 370 380 390 400
HPNICRLSPD GHFGSKFVTA VATGGPDNQV HFEGYQVSNQ CMALVRDECL
410 420 430 440 450
LPCKDAPELG YAKESSSEQY VPDVFYKDID KFGNEITQLA RPLPVEYLII
460 470 480 490 500
DITTTFPKDP VYTFSISQNP FPIENRDVLG ETQDFHSLAT YLSQNTSSVF
510 520 530 540 550
LDTISDFHLL LFLVTNEVMP LQDSISLLLE AVRTRNEELA QTWKKSEQWA
560 570 580 590 600
TIEQLCSTVG VQLPGLHEFG AVGGSARAAT SAMWACQHCT FMNQPGTGHC
EMCSLPRT
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LQX9 | F1LQX9_RAT | Nuclear protein localization protei... | Nploc4 | 608 | Annotation score: | ||
A0A0G2JU66 | A0A0G2JU66_RAT | Nuclear protein localization protei... | Nploc4 | 608 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF234600 mRNA Translation: AAG27534.1 BC101887 mRNA Translation: AAI01888.1 |
RefSeqi | NP_542144.1, NM_080577.2 |
Genome annotation databases
GeneIDi | 140639 |
KEGGi | rno:140639 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF234600 mRNA Translation: AAG27534.1 BC101887 mRNA Translation: AAI01888.1 |
RefSeqi | NP_542144.1, NM_080577.2 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
1NJ3 | NMR | - | A | 580-608 | [»] | |
1Q5W | NMR | - | A | 580-608 | [»] | |
SMRi | Q9ES54 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250817, 8 interactors |
ComplexPortali | CPX-138, Vcp-Npl4-Ufd1 AAA ATPase complex |
CORUMi | Q9ES54 |
DIPi | DIP-41365N |
IntActi | Q9ES54, 11 interactors |
MINTi | Q9ES54 |
STRINGi | 10116.ENSRNOP00000051856 |
PTM databases
iPTMneti | Q9ES54 |
PhosphoSitePlusi | Q9ES54 |
Proteomic databases
jPOSTi | Q9ES54 |
PaxDbi | Q9ES54 |
PRIDEi | Q9ES54 |
Genome annotation databases
GeneIDi | 140639 |
KEGGi | rno:140639 |
Organism-specific databases
CTDi | 55666 |
RGDi | 620794, Nploc4 |
Phylogenomic databases
eggNOGi | KOG2834, Eukaryota |
InParanoidi | Q9ES54 |
OrthoDBi | 1106766at2759 |
PhylomeDBi | Q9ES54 |
Enzyme and pathway databases
UniPathwayi | UPA00144 |
Reactomei | R-RNO-110320, Translesion Synthesis by POLH |
Miscellaneous databases
EvolutionaryTracei | Q9ES54 |
PROi | PR:Q9ES54 |
Family and domain databases
CDDi | cd08061, MPN_NPL4, 1 hit |
InterProi | View protein in InterPro IPR037518, MPN IPR016563, Npl4 IPR007717, NPL4_C IPR024682, Npl4_Ub-like_dom IPR007716, NPL4_Zn-bd_put IPR029071, Ubiquitin-like_domsf IPR001876, Znf_RanBP2 IPR036443, Znf_RanBP2_sf |
Pfami | View protein in Pfam PF05021, NPL4, 1 hit PF11543, UN_NPL4, 1 hit PF05020, zf-NPL4, 1 hit |
PIRSFi | PIRSF010052, Polyub_prc_Npl4, 1 hit |
SMARTi | View protein in SMART SM00547, ZnF_RBZ, 1 hit |
SUPFAMi | SSF54236, SSF54236, 1 hit SSF90209, SSF90209, 1 hit |
PROSITEi | View protein in PROSITE PS50249, MPN, 1 hit PS01358, ZF_RANBP2_1, 1 hit PS50199, ZF_RANBP2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | NPL4_RAT | |
Accessioni | Q9ES54Primary (citable) accession number: Q9ES54 Secondary accession number(s): Q3T1J3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 29, 2004 |
Last sequence update: | January 23, 2007 | |
Last modified: | December 2, 2020 | |
This is version 146 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Direct protein sequencing, Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families