UniProtKB - Q9ES07 (S15A2_MOUSE)
Protein
Solute carrier family 15 member 2
Gene
Slc15a2
Organism
Mus musculus (Mouse)
Status
Functioni
Proton-coupled amino-acid transporter that transports oligopeptides of 2 to 4 amino acids with a preference for dipeptides (PubMed:11027540). Transports the dipeptide-like aminopeptidase inhibitor bestatin (By similarity). Can also transport the aminocephalosporin antibiotic cefadroxil (By similarity). Also able to transport carnosine (By similarity). Involved in innate immunity by promoting the detection of microbial pathogens by NOD-like receptors (NLRs) (PubMed:29784761). Probably acts by mediating transport of bacterial peptidoglycans across the plasma membrane: catalyzes the transport of certain bacterial peptidoglycans, such as muramyl dipeptide (MDP), the NOD2 ligand (PubMed:29784761).By similarity2 Publications
Catalytic activityi
- This reaction proceeds in the forward2 Publications direction.
- H+(out) + N-acetylmuramyl-L-alanyl-D-isoglutamine(out) = H+(in) + N-acetylmuramyl-L-alanyl-D-isoglutamine(in)1 PublicationThis reaction proceeds in the forward1 Publication direction.
- This reaction proceeds in the forwardBy similarity direction.
- This reaction proceeds in the forwardBy similarity direction.
Kineticsi
- KM=56.6 µM for D-Phe-Ala (at pH 6.0)1 Publication
pH dependencei
Optimum pH is 6.0 or lower.1 Publication
GO - Molecular functioni
- dipeptide transmembrane transporter activity Source: UniProtKB
- high-affinity oligopeptide transmembrane transporter activity Source: MGI
- peptide:proton symporter activity Source: MGI
GO - Biological processi
- dipeptide import across plasma membrane Source: UniProtKB
- dipeptide transport Source: ARUK-UCL
- innate immune response Source: UniProtKB-KW
- oligopeptide transport Source: MGI
- peptidoglycan transport Source: UniProtKB
- protein transport Source: UniProtKB-KW
- regulation of nucleotide-binding oligomerization domain containing signaling pathway Source: UniProtKB
- renal absorption Source: ARUK-UCL
- xenobiotic transport Source: ARUK-UCL
Keywordsi
Biological process | Immunity, Innate immunity, Peptide transport, Protein transport, Symport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-427975, Proton/oligopeptide cotransporters |
Protein family/group databases
TCDBi | 2.A.17.4.4, the proton-dependent oligopeptide transporter (pot/ptr) family |
Names & Taxonomyi
Protein namesi | Recommended name: Solute carrier family 15 member 2CuratedAlternative name(s): Kidney H(+)/peptide cotransporterBy similarity Oligopeptide transporter, kidney isoformBy similarity Peptide transporter 21 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1890457, Slc15a2 |
Subcellular locationi
Plasma membrane
- Cell membrane 1 Publication1 Publication; Multi-pass membrane protein Sequence analysis
Plasma Membrane
- apical plasma membrane Source: MGI
- integral component of plasma membrane Source: MGI
Other locations
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 57 | CytoplasmicCuratedAdd BLAST | 57 | |
Transmembranei | 58 – 78 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 79 – 87 | ExtracellularCurated | 9 | |
Transmembranei | 88 – 108 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 109 – 113 | CytoplasmicCurated | 5 | |
Transmembranei | 114 – 134 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 135 – 139 | ExtracellularCurated | 5 | |
Transmembranei | 140 – 160 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 161 – 183 | CytoplasmicCuratedAdd BLAST | 23 | |
Transmembranei | 184 – 204 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 205 – 217 | ExtracellularCuratedAdd BLAST | 13 | |
Transmembranei | 218 – 238 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 239 – 295 | CytoplasmicCuratedAdd BLAST | 57 | |
Transmembranei | 296 – 316 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 317 – 343 | ExtracellularCuratedAdd BLAST | 27 | |
Transmembranei | 344 – 364 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 365 – 380 | CytoplasmicCuratedAdd BLAST | 16 | |
Transmembranei | 381 – 401 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 402 – 611 | ExtracellularCuratedAdd BLAST | 210 | |
Transmembranei | 612 – 632 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 633 – 643 | CytoplasmicCuratedAdd BLAST | 11 | |
Transmembranei | 644 – 664 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 665 – 674 | ExtracellularCurated | 10 | |
Transmembranei | 675 – 695 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 696 – 729 | CytoplasmicCuratedAdd BLAST | 34 |
Keywords - Cellular componenti
Cell membrane, MembranePathology & Biotechi
Disruption phenotypei
Decreased dipeptide uptake in bone marrow (PubMed:29784761). Decreased cytokine expression in response to LPS stimulation (PubMed:29784761).1 Publication
Chemistry databases
ChEMBLi | CHEMBL2073678 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064309 | 1 – 729 | Solute carrier family 15 member 2Add BLAST | 729 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 9 | PhosphoserineCombined sources | 1 | |
Modified residuei | 12 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 28 | PhosphoserineBy similarity | 1 | |
Glycosylationi | 448 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 472 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 528 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 | |
Glycosylationi | 587 | N-linked (GlcNAc...) asparaginePROSITE-ProRule annotation | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
jPOSTi | Q9ES07 |
MaxQBi | Q9ES07 |
PaxDbi | Q9ES07 |
PRIDEi | Q9ES07 |
PTM databases
GlyGeni | Q9ES07, 4 sites |
iPTMneti | Q9ES07 |
PhosphoSitePlusi | Q9ES07 |
SwissPalmi | Q9ES07 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Interacts (via extracellular domain region) with trypsin.
By similarityProtein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023616 |
Miscellaneous databases
RNActi | Q9ES07, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 402 – 611 | Extracellular domain (ECD)By similarityAdd BLAST | 210 |
Domaini
The extracellular domain (ECD) region specifically binds trypsin.By similarity
Sequence similaritiesi
Belongs to the major facilitator superfamily. Proton-dependent oligopeptide transporter (POT/PTR) (TC 2.A.17) family. [View classification]Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1237, Eukaryota |
InParanoidi | Q9ES07 |
PhylomeDBi | Q9ES07 |
Family and domain databases
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR036259, MFS_trans_sf IPR004768, Oligopep_transport IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 2 hits |
SUPFAMi | SSF103473, SSF103473, 1 hit SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00926, 2A1704, 1 hit |
PROSITEi | View protein in PROSITE PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q9ES07-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MNPFQKNESK ETLFSPVSTE EMLPGPPSPP KKSTPKLFGS SYPLSIAFIV
60 70 80 90 100
VNEFCERFSY YGMKAVLTLY FLYFLHWNED TSTSVYHAFS SLCYFTPILG
110 120 130 140 150
AAIADSWLGK FKTIIYLSLV YVLGHVFKSL GAIPILGGKM LHTILSLVGL
160 170 180 190 200
SLIALGTGGI KPCVAAFGGD QFEEEHAEAR TRYFSVFYLS INAGSLISTF
210 220 230 240 250
ITPMLRGDVK CFGEDCYALA FGIPGLLMVL ALVVFAMGSK MYRKPPPEGN
260 270 280 290 300
IVAQVTKCIW FAICNRFRNR SEDIPKRQHW LDWAAEKYPK HLIMDVKALT
310 320 330 340 350
RILFLYIPLP MFWALLDQQG SRWTLQANKM DGDLGFFVLQ PDQMQVLNPF
360 370 380 390 400
LVLVFIPLFD LVIYRLISKC GVNFSSLRKM AVGMILACLA FAVAALVEIK
410 420 430 440 450
INGMIHPQPA SQEIFLQVLN LADGEIEVTV QGNRNNPLLV ESISSFQNTT
460 470 480 490 500
HYSKLRLETK SQDLHFHLKY NNLSVHNEYS VEEKNCYQLV VHENGESLSS
510 520 530 540 550
MLVKDTGIKP ANGMTAIRFI NTLHKDMNIS LDANAPLSVG KDYGVSEYRT
560 570 580 590 600
VQRGKYPAVH CETEDNVFSL NLGQLDFGTT YLFVITNITN RGLQAWKAED
610 620 630 640 650
IPANKLSIAW QLPQYVLVTA AEVMFSVTGL EFSYSQAPSS MKSVLQAAWL
660 670 680 690 700
LTVAVGNIIV LIVAQFSGLV QWAEFVLFSC LLLVVCLIFS VMGYYYVPLK
710 720
SEGIHEATEK QIPHIQGNMI NLETKNTRL
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9QMN8 | E9QMN8_MOUSE | Solute carrier family 15 member 2 | Slc15a2 | 740 | Annotation score: | ||
E9PYQ9 | E9PYQ9_MOUSE | Solute carrier family 15 member 2 | Slc15a2 | 709 | Annotation score: | ||
E9Q329 | E9Q329_MOUSE | Solute carrier family 15 member 2 | Slc15a2 | 259 | Annotation score: | ||
G3XA51 | G3XA51_MOUSE | Solute carrier family 15 member 2 | Slc15a2 | 189 | Annotation score: | ||
E9Q0L2 | E9Q0L2_MOUSE | Solute carrier family 15 member 2 | Slc15a2 | 84 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF257711 Genomic DNA Translation: AAG25926.1 |
PIRi | JC7501 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF257711 Genomic DNA Translation: AAG25926.1 |
PIRi | JC7501 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000023616 |
Chemistry databases
ChEMBLi | CHEMBL2073678 |
Protein family/group databases
TCDBi | 2.A.17.4.4, the proton-dependent oligopeptide transporter (pot/ptr) family |
PTM databases
GlyGeni | Q9ES07, 4 sites |
iPTMneti | Q9ES07 |
PhosphoSitePlusi | Q9ES07 |
SwissPalmi | Q9ES07 |
Proteomic databases
jPOSTi | Q9ES07 |
MaxQBi | Q9ES07 |
PaxDbi | Q9ES07 |
PRIDEi | Q9ES07 |
Organism-specific databases
MGIi | MGI:1890457, Slc15a2 |
Phylogenomic databases
eggNOGi | KOG1237, Eukaryota |
InParanoidi | Q9ES07 |
PhylomeDBi | Q9ES07 |
Enzyme and pathway databases
Reactomei | R-MMU-427975, Proton/oligopeptide cotransporters |
Miscellaneous databases
ChiTaRSi | Slc15a2, mouse |
PROi | PR:Q9ES07 |
RNActi | Q9ES07, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR029028, Alpha/beta_knot_MTases IPR036259, MFS_trans_sf IPR004768, Oligopep_transport IPR000109, POT_fam IPR018456, PTR2_symporter_CS |
PANTHERi | PTHR11654, PTHR11654, 1 hit |
Pfami | View protein in Pfam PF00854, PTR2, 2 hits |
SUPFAMi | SSF103473, SSF103473, 1 hit SSF75217, SSF75217, 1 hit |
TIGRFAMsi | TIGR00926, 2A1704, 1 hit |
PROSITEi | View protein in PROSITE PS01022, PTR2_1, 1 hit PS01023, PTR2_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | S15A2_MOUSE | |
Accessioni | Q9ES07Primary (citable) accession number: Q9ES07 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 26, 2005 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 114 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families