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Protein

Ubiquitin conjugation factor E4 B

Gene

Ube4b

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (PubMed:11435423). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (By similarity).By similarity1 Publication

Miscellaneous

In strain C57BL/Ola, a 85 kb region on chromosome 4 containing Nmnat1 and Ube4b is triplicated. Ube4b becomes linked to Nmnat1 and encodes a fusion protein located in the nucleus which is responsible for the delayed Wallerian degeneration of injured axons in C57BL/Ola.1 Publication

Catalytic activityi

S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N6-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 Publication
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • ATPase binding Source: MGI
  • ATP binding Source: MGI
  • enzyme binding Source: UniProtKB
  • ubiquitin protein ligase activity Source: MGI
  • ubiquitin-ubiquitin ligase activity Source: MGI
  • unfolded protein binding Source: UniProtKB

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi2.3.2.B12 3474
UniPathwayi
UPA00143

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin conjugation factor E4 BCurated (EC:2.3.2.271 Publication)
Alternative name(s):
RING-type E3 ubiquitin transferase E4 B
Ubiquitin fusion degradation protein 21 Publication
Gene namesi
Name:Ube4bImported
Synonyms:Ufd2Imported, Ufd2aImported
OrganismiMus musculus (Mouse)Imported
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 4

Organism-specific databases

MGIiMGI:1927086 Ube4b

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi1140P → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001949941 – 1173Ubiquitin conjugation factor E4 BAdd BLAST1173

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei23PhosphoserineBy similarity1
Modified residuei31PhosphoserineCombined sources1
Modified residuei84PhosphoserineBy similarity1
Modified residuei88PhosphoserineCombined sources1
Modified residuei90PhosphoserineCombined sources1
Modified residuei101PhosphoserineCombined sources1
Modified residuei103PhosphoserineCombined sources1
Modified residuei105PhosphoserineBy similarity1
Modified residuei124PhosphoserineBy similarity1
Modified residuei238PhosphoserineBy similarity1
Modified residuei674PhosphoserineCombined sources1
Modified residuei840PhosphoserineCombined sources1
Modified residuei1136PhosphoserineBy similarity1

Post-translational modificationi

Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7 (By similarity).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei109 – 110Cleavage; by caspase-3 and caspase-7By similarity2
Sitei123 – 124Cleavage; by caspase-6 and granzyme BBy similarity2

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9ES00
MaxQBiQ9ES00
PaxDbiQ9ES00
PeptideAtlasiQ9ES00
PRIDEiQ9ES00

PTM databases

iPTMnetiQ9ES00
PhosphoSitePlusiQ9ES00

Expressioni

Tissue specificityi

Expressed predominantly in neuronal tissues. Also detected in liver, heart, brain, kidney and testis.2 Publications

Gene expression databases

BgeeiENSMUSG00000028960 Expressed in 335 organ(s), highest expression level in cumulus cell
ExpressionAtlasiQ9ES00 baseline and differential
GenevisibleiQ9ES00 MM

Interactioni

Subunit structurei

Interacts with VCP (PubMed:12504083). Interacts with STUB1/CHIP and UNC45B (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211009, 8 interactors
IntActiQ9ES00, 2 interactors
STRINGi10090.ENSMUSP00000099501

Structurei

Secondary structure

11173
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9ES00
SMRiQ9ES00
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini1098 – 1171U-boxAdd BLAST74

Domaini

The U-box domain is required for the ubiquitin protein ligase activity.1 Publication

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2042 Eukaryota
COG5113 LUCA
GeneTreeiENSGT00390000009300
HOVERGENiHBG058129
InParanoidiQ9ES00
KOiK10597
OMAiAMWNHEL
OrthoDBiEOG091G02B4
TreeFamiTF300802

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR019474 Ub_conjug_fac_E4_core
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
PF10408 Ufd2P_core, 1 hit
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9ES00-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS
60 70 80 90 100
QSLGLNVHNM TPATSPIGAA GVAHRSQSSE GVSSLSSSPS NSLETQSQSL
110 120 130 140 150
SRSQSMDIDG VSCEKSMSQV DVDSGIENME VDENDRREKR SLSDKEPSSG
160 170 180 190 200
PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL SSLSAQFKQN PKEVFSDFKD
210 220 230 240 250
LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIATAARSPD RNLMLNTGSS
260 270 280 290 300
SGTSPMFCNM GSFSTSSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY
310 320 330 340 350
LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP
360 370 380 390 400
RSLQQPSFLV PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL
410 420 430 440 450
AAKECSLESD YFKYPLMALG ELCETKFGKT HPMCNLVASL PLWLPKSLSP
460 470 480 490 500
GSGRELQRLS YLGAFFSFSV FAEDDAKVVE KYFSGPAITL ENTRVVSQSL
510 520 530 540 550
QHYLELGRQE LFKILHSILL NGETREAALS YMAALVNANM KKAQMQADDR
560 570 580 590 600
LVSTDGFMLN LLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRINA
610 620 630 640 650
TMEDVNERLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR
660 670 680 690 700
LRAIRELNRT VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC
710 720 730 740 750
ADAGLLDESF LRRCLNFYGL LIQLMLRILD PAYPDVTLPL NSEVPKVFAA
760 770 780 790 800
LPEFYVEDVA EFLFFIVQYS PQVLYEPCTQ DIVMFLVVML CNQNYIRNPY
810 820 830 840 850
LVAKLVEVMF MTNPSVQPRT QKFFEMIENH PLSTKLLVPS LMKFYTDVEH
860 870 880 890 900
TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM
910 920 930 940 950
LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD
960 970 980 990 1000
ERVSRSYLAL ATETVDMFHL LTKQVQKPFL RPELGPRLAA MLNFNLQQLC
1010 1020 1030 1040 1050
GPKCRDLKVE NPEKYGFEPK KLLDQLTDIY LQLDCARFAK AIADDQRSYS
1060 1070 1080 1090 1100
KELFEEVISK MRKAGIKSTI AIEKFKLLAE KVEEIVAKNA RAEIDYSDAP
1110 1120 1130 1140 1150
DEFRDPLMDT LMTDPVRLPS GTVMDRSIIL RHLLNSPTDP FNRQMLTESM
1160 1170
LEPVPELKEQ IQAWMREKQS SDH
Length:1,173
Mass (Da):133,318
Last modified:July 27, 2011 - v3
Checksum:iFC4879E47672E983
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UZD6G3UZD6_MOUSE
Ubiquitin conjugation factor E4 B
Ube4b
998Annotation score:
H7BXC2H7BXC2_MOUSE
Ubiquitin conjugation factor E4 B
Ube4b
128Annotation score:
G3UZM6G3UZM6_MOUSE
Ubiquitin conjugation factor E4 B
Ube4b
409Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti298L → P in AAG17287 (PubMed:11027338).Curated1
Sequence conflicti408E → D in AAG17287 (PubMed:11027338).Curated1
Sequence conflicti408E → D in BAC56586 (PubMed:12504083).Curated1
Sequence conflicti674S → T in BAC26672 (PubMed:16141072).Curated1
Sequence conflicti697C → Y in AAG17287 (PubMed:11027338).Curated1
Sequence conflicti753E → K in BAC26672 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260924 mRNA Translation: AAG17285.1 Different termination.
AF260926 mRNA Translation: AAG17287.1
AF260927 Genomic DNA Translation: AAG38492.1
AB083274 Genomic DNA Translation: BAC56586.1
AK029914 mRNA Translation: BAC26672.1
AL606973 Genomic DNA Translation: CAM16303.1
BC075620 mRNA Translation: AAH75620.1
CCDSiCCDS18958.1
RefSeqiNP_071305.2, NM_022022.3
UniGeneiMm.415260
Mm.478205

Genome annotation databases

EnsembliENSMUST00000103212; ENSMUSP00000099501; ENSMUSG00000028960
GeneIDi63958
KEGGimmu:63958
UCSCiuc012dpr.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF260924 mRNA Translation: AAG17285.1 Different termination.
AF260926 mRNA Translation: AAG17287.1
AF260927 Genomic DNA Translation: AAG38492.1
AB083274 Genomic DNA Translation: BAC56586.1
AK029914 mRNA Translation: BAC26672.1
AL606973 Genomic DNA Translation: CAM16303.1
BC075620 mRNA Translation: AAH75620.1
CCDSiCCDS18958.1
RefSeqiNP_071305.2, NM_022022.3
UniGeneiMm.415260
Mm.478205

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2KR4NMR-A1092-1173[»]
ProteinModelPortaliQ9ES00
SMRiQ9ES00
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211009, 8 interactors
IntActiQ9ES00, 2 interactors
STRINGi10090.ENSMUSP00000099501

PTM databases

iPTMnetiQ9ES00
PhosphoSitePlusiQ9ES00

Proteomic databases

EPDiQ9ES00
MaxQBiQ9ES00
PaxDbiQ9ES00
PeptideAtlasiQ9ES00
PRIDEiQ9ES00

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000103212; ENSMUSP00000099501; ENSMUSG00000028960
GeneIDi63958
KEGGimmu:63958
UCSCiuc012dpr.1 mouse

Organism-specific databases

CTDi10277
MGIiMGI:1927086 Ube4b

Phylogenomic databases

eggNOGiKOG2042 Eukaryota
COG5113 LUCA
GeneTreeiENSGT00390000009300
HOVERGENiHBG058129
InParanoidiQ9ES00
KOiK10597
OMAiAMWNHEL
OrthoDBiEOG091G02B4
TreeFamiTF300802

Enzyme and pathway databases

UniPathwayi
UPA00143

BRENDAi2.3.2.B12 3474

Miscellaneous databases

ChiTaRSiUbe4b mouse
PROiPR:Q9ES00
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000028960 Expressed in 335 organ(s), highest expression level in cumulus cell
ExpressionAtlasiQ9ES00 baseline and differential
GenevisibleiQ9ES00 MM

Family and domain databases

Gene3Di3.30.40.10, 1 hit
InterProiView protein in InterPro
IPR019474 Ub_conjug_fac_E4_core
IPR003613 Ubox_domain
IPR013083 Znf_RING/FYVE/PHD
PfamiView protein in Pfam
PF04564 U-box, 1 hit
PF10408 Ufd2P_core, 1 hit
SMARTiView protein in SMART
SM00504 Ubox, 1 hit
PROSITEiView protein in PROSITE
PS51698 U_BOX, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiUBE4B_MOUSE
AccessioniPrimary (citable) accession number: Q9ES00
Secondary accession number(s): Q6DID4, Q9EQE0
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 4, 2003
Last sequence update: July 27, 2011
Last modified: September 12, 2018
This is version 140 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  4. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
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