UniProtKB - Q9ES00 (UBE4B_MOUSE)
Protein
Ubiquitin conjugation factor E4 B
Gene
Ube4b
Organism
Mus musculus (Mouse)
Status
Functioni
Ubiquitin-protein ligase that probably functions as an E3 ligase in conjunction with specific E1 and E2 ligases (PubMed:11435423). May also function as an E4 ligase mediating the assembly of polyubiquitin chains on substrates ubiquitinated by another E3 ubiquitin ligase (By similarity). May regulate myosin assembly in striated muscles together with STUB1 and VCP/p97 by targeting myosin chaperone UNC45B for proteasomal degradation (By similarity).By similarity1 Publication
Miscellaneous
In strain C57BL/Ola, a 85 kb region on chromosome 4 containing Nmnat1 and Ube4b is triplicated. Ube4b becomes linked to Nmnat1 and encodes a fusion protein located in the nucleus which is responsible for the delayed Wallerian degeneration of injured axons in C57BL/Ola.1 Publication
Catalytic activityi
- S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine + [acceptor protein]-L-lysine = [E2 ubiquitin-conjugating enzyme]-L-cysteine + N(6)-ubiquitinyl-[acceptor protein]-L-lysine.1 Publication EC:2.3.2.27
: protein ubiquitination Pathwayi
This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.1 PublicationView all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.
GO - Molecular functioni
- ATPase binding Source: MGI
- ATP binding Source: MGI
- enzyme binding Source: UniProtKB
- ubiquitin protein ligase activity Source: MGI
- ubiquitin-ubiquitin ligase activity Source: MGI
- unfolded protein binding Source: UniProtKB
GO - Biological processi
- cellular protein catabolic process Source: MGI
- granzyme-mediated apoptotic signaling pathway Source: UniProtKB
- neuron projection development Source: MGI
- proteasome-mediated ubiquitin-dependent protein catabolic process Source: MGI
- protein autoubiquitination Source: MGI
- protein folding Source: UniProtKB
- protein monoubiquitination Source: MGI
- protein polyubiquitination Source: MGI
- protein ubiquitination Source: MGI
- response to endoplasmic reticulum stress Source: MGI
- response to UV Source: UniProtKB
- ubiquitin-dependent ERAD pathway Source: GO_Central
- ubiquitin-dependent protein catabolic process Source: MGI
- ventricular trabecula myocardium morphogenesis Source: MGI
Keywordsi
Molecular function | Transferase |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
BRENDAi | 2.3.2.B12, 3474 |
UniPathwayi | UPA00143 |
Names & Taxonomyi
Protein namesi | Recommended name: Ubiquitin conjugation factor E4 BCurated (EC:2.3.2.271 Publication)Alternative name(s): RING-type E3 ubiquitin transferase E4 B Ubiquitin fusion degradation protein 21 Publication |
Gene namesi | |
Organismi | Mus musculus (Mouse)Imported |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1927086, Ube4b |
Subcellular locationi
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: UniProtKB
- ubiquitin ligase complex Source: UniProtKB
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 1140 | P → A: Loss of E3 ubiquitin protein ligase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000194994 | 1 – 1173 | Ubiquitin conjugation factor E4 BAdd BLAST | 1173 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 23 | PhosphoserineBy similarity | 1 | |
Modified residuei | 31 | PhosphoserineCombined sources | 1 | |
Modified residuei | 84 | PhosphoserineBy similarity | 1 | |
Modified residuei | 88 | PhosphoserineCombined sources | 1 | |
Modified residuei | 90 | PhosphoserineCombined sources | 1 | |
Modified residuei | 101 | PhosphoserineCombined sources | 1 | |
Modified residuei | 103 | PhosphoserineCombined sources | 1 | |
Modified residuei | 105 | PhosphoserineBy similarity | 1 | |
Modified residuei | 124 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphoserineBy similarity | 1 | |
Modified residuei | 674 | PhosphoserineCombined sources | 1 | |
Modified residuei | 840 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1136 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Proteolytically cleaved by caspases during apoptosis. Cleaved efficiently at Asp-123 by caspase-6 and granzyme B. Cleaved with approximately 10-fold less efficiency at Asp-109 by caspase-3 and caspase-7 (By similarity).By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sitei | 109 – 110 | Cleavage; by caspase-3 and caspase-7By similarity | 2 | |
Sitei | 123 – 124 | Cleavage; by caspase-6 and granzyme BBy similarity | 2 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9ES00 |
jPOSTi | Q9ES00 |
MaxQBi | Q9ES00 |
PaxDbi | Q9ES00 |
PeptideAtlasi | Q9ES00 |
PRIDEi | Q9ES00 |
PTM databases
iPTMneti | Q9ES00 |
PhosphoSitePlusi | Q9ES00 |
Expressioni
Tissue specificityi
Expressed predominantly in neuronal tissues. Also detected in liver, heart, brain, kidney and testis.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000028960, Expressed in embryonic post-anal tail and 348 other tissues |
ExpressionAtlasi | Q9ES00, baseline and differential |
Genevisiblei | Q9ES00, MM |
Interactioni
Subunit structurei
Interacts with VCP (PubMed:12504083).
Interacts with STUB1/CHIP and UNC45B (By similarity).
By similarity1 PublicationGO - Molecular functioni
- ATPase binding Source: MGI
- enzyme binding Source: UniProtKB
- unfolded protein binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 211009, 19 interactors |
IntActi | Q9ES00, 2 interactors |
STRINGi | 10090.ENSMUSP00000099501 |
Miscellaneous databases
RNActi | Q9ES00, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
BMRBi | Q9ES00 |
SMRi | Q9ES00 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1098 – 1171 | U-boxAdd BLAST | 74 |
Domaini
The U-box domain is required for the ubiquitin protein ligase activity.1 Publication
Sequence similaritiesi
Belongs to the ubiquitin conjugation factor E4 family.Curated
Phylogenomic databases
eggNOGi | KOG2042, Eukaryota |
GeneTreei | ENSGT00390000009300 |
HOGENOMi | CLU_003224_2_0_1 |
InParanoidi | Q9ES00 |
OMAi | AMWNHEL |
OrthoDBi | 194967at2759 |
TreeFami | TF300802 |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR019474, Ub_conjug_fac_E4_core IPR003613, Ubox_domain IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF04564, U-box, 1 hit PF10408, Ufd2P_core, 1 hit |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 5 potential isoforms that are computationally mapped.Show allAlign All
Q9ES00-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MEELSADEIR RRRLARLAGG QTSQPTTPLT SPQRENPPGP PIAASAPGPS
60 70 80 90 100
QSLGLNVHNM TPATSPIGAA GVAHRSQSSE GVSSLSSSPS NSLETQSQSL
110 120 130 140 150
SRSQSMDIDG VSCEKSMSQV DVDSGIENME VDENDRREKR SLSDKEPSSG
160 170 180 190 200
PEVSEEQALQ LVCKIFRVSW KDRDRDVIFL SSLSAQFKQN PKEVFSDFKD
210 220 230 240 250
LIGQILMEVL MMSTQTRDEN PFASLTATSQ PIATAARSPD RNLMLNTGSS
260 270 280 290 300
SGTSPMFCNM GSFSTSSLSS LGASGGASNW DSYSDHFTIE TCKETDMLNY
310 320 330 340 350
LIECFDRVGI EEKKAPKMCS QPAVSQLLSN IRSQCISHTA LVLQGSLTQP
360 370 380 390 400
RSLQQPSFLV PYMLCRNLPY GFIQELVRTT HQDEEVFKQI FIPILQGLAL
410 420 430 440 450
AAKECSLESD YFKYPLMALG ELCETKFGKT HPMCNLVASL PLWLPKSLSP
460 470 480 490 500
GSGRELQRLS YLGAFFSFSV FAEDDAKVVE KYFSGPAITL ENTRVVSQSL
510 520 530 540 550
QHYLELGRQE LFKILHSILL NGETREAALS YMAALVNANM KKAQMQADDR
560 570 580 590 600
LVSTDGFMLN LLWVLQQLST KIKLETVDPT YIFHPRCRIT LPNDETRINA
610 620 630 640 650
TMEDVNERLT ELYGDQPPFS EPKFPTECFF LTLHAHHLSI LPSCRRYIRR
660 670 680 690 700
LRAIRELNRT VEDLKNNESQ WKDSPLATRH REMLKRCKTQ LKKLVRCKAC
710 720 730 740 750
ADAGLLDESF LRRCLNFYGL LIQLMLRILD PAYPDVTLPL NSEVPKVFAA
760 770 780 790 800
LPEFYVEDVA EFLFFIVQYS PQVLYEPCTQ DIVMFLVVML CNQNYIRNPY
810 820 830 840 850
LVAKLVEVMF MTNPSVQPRT QKFFEMIENH PLSTKLLVPS LMKFYTDVEH
860 870 880 890 900
TGATSEFYDK FTIRYHISTI FKSLWQNIAH HGTFMEEFNS GKQFVRYINM
910 920 930 940 950
LINDTTFLLD ESLESLKRIH EVQEEMKNKE QWDQLPRDQQ QARQSQLAQD
960 970 980 990 1000
ERVSRSYLAL ATETVDMFHL LTKQVQKPFL RPELGPRLAA MLNFNLQQLC
1010 1020 1030 1040 1050
GPKCRDLKVE NPEKYGFEPK KLLDQLTDIY LQLDCARFAK AIADDQRSYS
1060 1070 1080 1090 1100
KELFEEVISK MRKAGIKSTI AIEKFKLLAE KVEEIVAKNA RAEIDYSDAP
1110 1120 1130 1140 1150
DEFRDPLMDT LMTDPVRLPS GTVMDRSIIL RHLLNSPTDP FNRQMLTESM
1160 1170
LEPVPELKEQ IQAWMREKQS SDH
Computationally mapped potential isoform sequencesi
There are 5 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A571BD95 | A0A571BD95_MOUSE | Ubiquitin conjugation factor E4 B | Ube4b | 1,307 | Annotation score: | ||
A0A5F8MP95 | A0A5F8MP95_MOUSE | Ubiquitin conjugation factor E4 B | Ube4b | 1,353 | Annotation score: | ||
G3UZD6 | G3UZD6_MOUSE | Ubiquitin conjugation factor E4 B | Ube4b | 998 | Annotation score: | ||
H7BXC2 | H7BXC2_MOUSE | Ubiquitin conjugation factor E4 B | Ube4b | 128 | Annotation score: | ||
G3UZM6 | G3UZM6_MOUSE | Ubiquitin conjugation factor E4 B | Ube4b | 409 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 298 | L → P in AAG17287 (PubMed:11027338).Curated | 1 | |
Sequence conflicti | 408 | E → D in AAG17287 (PubMed:11027338).Curated | 1 | |
Sequence conflicti | 408 | E → D in BAC56586 (PubMed:12504083).Curated | 1 | |
Sequence conflicti | 674 | S → T in BAC26672 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 697 | C → Y in AAG17287 (PubMed:11027338).Curated | 1 | |
Sequence conflicti | 753 | E → K in BAC26672 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260924 mRNA Translation: AAG17285.1 Different termination. AF260926 mRNA Translation: AAG17287.1 AF260927 Genomic DNA Translation: AAG38492.1 AB083274 Genomic DNA Translation: BAC56586.1 AK029914 mRNA Translation: BAC26672.1 AL606973 Genomic DNA No translation available. BC075620 mRNA Translation: AAH75620.1 |
CCDSi | CCDS18958.1 |
RefSeqi | NP_071305.2, NM_022022.3 |
Genome annotation databases
Ensembli | ENSMUST00000103212; ENSMUSP00000099501; ENSMUSG00000028960 |
GeneIDi | 63958 |
KEGGi | mmu:63958 |
UCSCi | uc012dpr.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF260924 mRNA Translation: AAG17285.1 Different termination. AF260926 mRNA Translation: AAG17287.1 AF260927 Genomic DNA Translation: AAG38492.1 AB083274 Genomic DNA Translation: BAC56586.1 AK029914 mRNA Translation: BAC26672.1 AL606973 Genomic DNA No translation available. BC075620 mRNA Translation: AAH75620.1 |
CCDSi | CCDS18958.1 |
RefSeqi | NP_071305.2, NM_022022.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2KR4 | NMR | - | A | 1092-1173 | [»] | |
BMRBi | Q9ES00 | |||||
SMRi | Q9ES00 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 211009, 19 interactors |
IntActi | Q9ES00, 2 interactors |
STRINGi | 10090.ENSMUSP00000099501 |
PTM databases
iPTMneti | Q9ES00 |
PhosphoSitePlusi | Q9ES00 |
Proteomic databases
EPDi | Q9ES00 |
jPOSTi | Q9ES00 |
MaxQBi | Q9ES00 |
PaxDbi | Q9ES00 |
PeptideAtlasi | Q9ES00 |
PRIDEi | Q9ES00 |
Protocols and materials databases
Antibodypediai | 13520, 169 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000103212; ENSMUSP00000099501; ENSMUSG00000028960 |
GeneIDi | 63958 |
KEGGi | mmu:63958 |
UCSCi | uc012dpr.1, mouse |
Organism-specific databases
CTDi | 10277 |
MGIi | MGI:1927086, Ube4b |
Phylogenomic databases
eggNOGi | KOG2042, Eukaryota |
GeneTreei | ENSGT00390000009300 |
HOGENOMi | CLU_003224_2_0_1 |
InParanoidi | Q9ES00 |
OMAi | AMWNHEL |
OrthoDBi | 194967at2759 |
TreeFami | TF300802 |
Enzyme and pathway databases
UniPathwayi | UPA00143 |
BRENDAi | 2.3.2.B12, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 63958, 7 hits in 19 CRISPR screens |
ChiTaRSi | Ube4b, mouse |
PROi | PR:Q9ES00 |
RNActi | Q9ES00, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028960, Expressed in embryonic post-anal tail and 348 other tissues |
ExpressionAtlasi | Q9ES00, baseline and differential |
Genevisiblei | Q9ES00, MM |
Family and domain databases
Gene3Di | 3.30.40.10, 1 hit |
InterProi | View protein in InterPro IPR019474, Ub_conjug_fac_E4_core IPR003613, Ubox_domain IPR013083, Znf_RING/FYVE/PHD |
Pfami | View protein in Pfam PF04564, U-box, 1 hit PF10408, Ufd2P_core, 1 hit |
SMARTi | View protein in SMART SM00504, Ubox, 1 hit |
PROSITEi | View protein in PROSITE PS51698, U_BOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | UBE4B_MOUSE | |
Accessioni | Q9ES00Primary (citable) accession number: Q9ES00 Secondary accession number(s): Q6DID4, Q9EQE0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | April 4, 2003 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families