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Protein

Transient receptor potential cation channel subfamily V member 4

Gene

Trpv4

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Non-selective calcium permeant cation channel involved in osmotic sensitivity and mechanosensitivity (PubMed:11081638). Activation by exposure to hypotonicity within the physiological range exhibits an outward rectification (PubMed:11081638). Also activated by heat, low pH, citrate and phorbol esters (By similarity). Increase of intracellular Ca2+ potentiates currents (By similarity). Channel activity seems to be regulated by a calmodulin-dependent mechanism with a negative feedback mechanism (By similarity). Acts as a regulator of intracellular Ca2+ in synoviocytes (By similarity). Plays an obligatory role as a molecular component in the nonselective cation channel activation induced by 4-alpha-phorbol 12,13-didecanoate and hypotonic stimulation in synoviocytes and also regulates production of IL-8 (By similarity). Together with PKD2, forms mechano- and thermosensitive channels in cilium (By similarity). Promotes cell-cell junction formation in skin keratinocytes and plays an important role in the formation and/or maintenance of functional intercellular barriers (By similarity). Negatively regulates expression of PPARGC1A, UCP1, oxidative metabolism and respiration in adipocytes (By similarity). Regulates expression of chemokines and cytokines related to proinflammatory pathway in adipocytes (By similarity). Together with AQP5, controls regulatory volume decrease in salivary epithelial cells (By similarity). Required for normal development and maintenance of bone and cartilage (By similarity).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei192ATPBy similarity1
Binding sitei197ATPBy similarity1
Binding sitei201ATPBy similarity1
Binding sitei248ATPBy similarity1
Binding sitei344Phosphatidylinositol-1,4,5-trisphosphateBy similarity1
Metal bindingi682Calcium; shared with neighboring subunitsBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi236 – 239ATPBy similarity4

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionCalcium channel, Calmodulin-binding, Ion channel
Biological processCalcium transport, Ion transport, Transport
LigandATP-binding, Calcium, Lipid-binding, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-RNO-3295583 TRP channels

Protein family/group databases

TCDBi1.A.4.2.5 the transient receptor potential ca(2+) channel (trp-cc) family

Names & Taxonomyi

Protein namesi
Recommended name:
Transient receptor potential cation channel subfamily V member 4
Short name:
TrpV4
Alternative name(s):
Osm-9-like TRP channel 4
Short name:
OTRPC4
Vanilloid receptor-related osmotically-activated channel1 Publication
Short name:
VR-OAC1 Publication
Gene namesi
Name:Trpv4
Synonyms:Vroac
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 12

Organism-specific databases

RGDi69337 Trpv4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 469CytoplasmicBy similarityAdd BLAST469
Transmembranei470 – 490HelicalBy similarityAdd BLAST21
Topological domaini491 – 507ExtracellularBy similarityAdd BLAST17
Transmembranei508 – 534HelicalBy similarityAdd BLAST27
Topological domaini535 – 547CytoplasmicBy similarityAdd BLAST13
Transmembranei548 – 568HelicalBy similarityAdd BLAST21
Topological domaini569 – 572ExtracellularBy similarity4
Transmembranei573 – 593HelicalBy similarityAdd BLAST21
Topological domaini594 – 608CytoplasmicBy similarityAdd BLAST15
Transmembranei609 – 636HelicalBy similarityAdd BLAST28
Topological domaini637 – 665ExtracellularBy similarityAdd BLAST29
Intramembranei666 – 685Pore-formingBy similarityAdd BLAST20
Topological domaini686 – 693ExtracellularBy similarity8
Transmembranei694 – 722HelicalBy similarityAdd BLAST29
Topological domaini723 – 871CytoplasmicBy similarityAdd BLAST149

Keywords - Cellular componenti

Cell junction, Cell membrane, Cell projection, Cilium, Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2775
GuidetoPHARMACOLOGYi510

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002153491 – 871Transient receptor potential cation channel subfamily V member 4Add BLAST871

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei110PhosphotyrosineBy similarity1
Modified residuei253PhosphotyrosineBy similarity1
Modified residuei805PhosphotyrosineBy similarity1
Modified residuei824PhosphoserineBy similarity1

Post-translational modificationi

N-glycosylated.By similarity

Keywords - PTMi

Glycoprotein, Phosphoprotein

Proteomic databases

PaxDbiQ9ERZ8
PRIDEiQ9ERZ8

PTM databases

PhosphoSitePlusiQ9ERZ8

Expressioni

Tissue specificityi

Expressed lung, spleen, kidney, testis, fat, and at very low levels in trigeminal ganglia.1 Publication

Gene expression databases

BgeeiENSRNOG00000001195 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
GenevisibleiQ9ERZ8 RN

Interactioni

Subunit structurei

Homotetramer. Interacts with calmodulin (By similarity). Interacts with MAP7 and Src family Tyr protein kinases LYN, SRC, FYN, HCK, LCK and YES (By similarity). Interacts with CTNNB1 (By similarity). The TRPV4 and CTNNB1 complex can interact with CDH1 (By similarity). Part of a complex containing MLC1, AQP4, HEPACAM and ATP1B1 (By similarity). Interacts with PACSIN1, PACSIN2 and PACSIN3 (via SH3 domain) (PubMed:16627472). Interacts with ITPR3 (By similarity). Interacts with AQP5; the interaction is probably indirect and regulates TRPV4 activation by hypotonicity (By similarity). Interacts with ANO1 (By similarity). Interacts (via C-terminus) with PKD2 (via C-terminus) (By similarity).By similarity1 Publication

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi249353, 2 interactors
DIPiDIP-59600N
IntActiQ9ERZ8, 3 interactors
STRINGi10116.ENSRNOP00000001586

Chemistry databases

BindingDBiQ9ERZ8

Structurei

3D structure databases

ProteinModelPortaliQ9ERZ8
SMRiQ9ERZ8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati237 – 266ANK 1Add BLAST30
Repeati284 – 313ANK 2Add BLAST30
Repeati369 – 398ANK 3Add BLAST30

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni249 – 251Phosphatidylinositol-1,4,5-trisphosphate bindingBy similarity3
Regioni296 – 299Phosphatidylinositol-1,4,5-trisphosphate bindingBy similarity4
Regioni812 – 831Interaction with calmodulin and ITPR3By similarityAdd BLAST20

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi679 – 682Selectivity filterBy similarity4

Domaini

The ANK repeat region mediates interaction with Ca2+-calmodulin and ATP binding. The ANK repeat region mediates interaction with phosphatidylinositol-4,5-bisphosphate and related phosphatidylinositides.By similarity

Sequence similaritiesi

Keywords - Domaini

ANK repeat, Repeat, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3676 Eukaryota
ENOG4110DG4 LUCA
GeneTreeiENSGT00550000074425
HOGENOMiHOG000234630
HOVERGENiHBG054085
InParanoidiQ9ERZ8
KOiK04973
OMAiKVCNEDQ
OrthoDBiEOG091G016O
PhylomeDBiQ9ERZ8
TreeFamiTF314711

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR004729 TRP_channel
IPR024862 TRPV
IPR008347 TRPV1-4_channel
IPR008348 TRPV4_channel
PANTHERiPTHR10582 PTHR10582, 1 hit
PTHR10582:SF4 PTHR10582:SF4, 1 hit
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR01768 TRPVRECEPTOR
PR01769 VRL2RECEPTOR
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
TIGRFAMsiTIGR00870 trp, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit

Sequencei

Sequence statusi: Complete.

Q9ERZ8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MADPGDGPRA APGDVAEPPG DESGTSGGEA FPLSSLANLF EGEEGSSSLS
60 70 80 90 100
PVDASRPAGP GDGRPNLRMK FQGAFRKGVP NPIDLLESTL YESSVVPGPK
110 120 130 140 150
KAPMDSLFDY GTYRHHPSDN KRWRRKVVEK QPQSPKAPAP QPPPILKVFN
160 170 180 190 200
RPILFDIVSR GSTADLDGLL SYLLTHKKRL TDEEFREPST GKTCLPKALL
210 220 230 240 250
NLSNGRNDTI PVLLDIAERT GNMREFINSP FRDIYYRGQT ALHIAIERRC
260 270 280 290 300
KHYVELLVAQ GADVHAQARG RFFQPKDEGG YFYFGELPLS LAACTNQPHI
310 320 330 340 350
VNYLTENPHK KADMRRQDSR GNTVLHALVA IADNTRENTK FVTKMYDLLL
360 370 380 390 400
LKCSRLFPDS NLETVLNNDG LSPLMMAAKT GKIGVFQHII RREVTDEDTR
410 420 430 440 450
HLSRKFKDWA YGPVYSSLYD LSSLDTCGEE VSVLEILVYN SKIENRHEML
460 470 480 490 500
AVEPINELLR DKWRKFGAVS FYINVVSYLC AMVIFTLTAY YQPLEGTPPY
510 520 530 540 550
PYRTTVDYLR LAGEVITLLT GVLFFFTSIK DLFMKKCPGV NSLFVDGSFQ
560 570 580 590 600
LLYFIYSVLV VVSAALYLAG IEAYLAVMVF ALVLGWMNAL YFTRGLKLTG
610 620 630 640 650
TYSIMIQKIL FKDLFRFLLV YLLFMIGYAS ALVTLLNPCT NMKVCNEDQS
660 670 680 690 700
NCTVPSYPAC RDSETFSAFL LDLFKLTIGM GDLEMLSSAK YPVVFILLLV
710 720 730 740 750
TYIILTFVLL LNMLIALMGE TVGQVSKESK HIWKLQWATT ILDIERSFPV
760 770 780 790 800
FLRKAFRSGE MVTVGKSSDG TPDRRWCFRV DEVNWSHWNQ NLGIINEDPG
810 820 830 840 850
KSEIYQYYGF SHTMGRLRRD RWSSVVPRVV ELNKNSGTDE VVVPLDNLGN
860 870
PNCDGHQQGY APKWRAEDAP L
Length:871
Mass (Da):98,010
Last modified:March 1, 2001 - v1
Checksum:i5D50684DA08C354B
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263521 mRNA Translation: AAG28027.1
RefSeqiNP_076460.1, NM_023970.1
XP_006249528.1, XM_006249466.2
UniGeneiRn.64508

Genome annotation databases

EnsembliENSRNOT00000001586; ENSRNOP00000001586; ENSRNOG00000001195
GeneIDi66026
KEGGirno:66026
UCSCiRGD:69337 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF263521 mRNA Translation: AAG28027.1
RefSeqiNP_076460.1, NM_023970.1
XP_006249528.1, XM_006249466.2
UniGeneiRn.64508

3D structure databases

ProteinModelPortaliQ9ERZ8
SMRiQ9ERZ8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249353, 2 interactors
DIPiDIP-59600N
IntActiQ9ERZ8, 3 interactors
STRINGi10116.ENSRNOP00000001586

Chemistry databases

BindingDBiQ9ERZ8
ChEMBLiCHEMBL2775
GuidetoPHARMACOLOGYi510

Protein family/group databases

TCDBi1.A.4.2.5 the transient receptor potential ca(2+) channel (trp-cc) family

PTM databases

PhosphoSitePlusiQ9ERZ8

Proteomic databases

PaxDbiQ9ERZ8
PRIDEiQ9ERZ8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000001586; ENSRNOP00000001586; ENSRNOG00000001195
GeneIDi66026
KEGGirno:66026
UCSCiRGD:69337 rat

Organism-specific databases

CTDi59341
RGDi69337 Trpv4

Phylogenomic databases

eggNOGiKOG3676 Eukaryota
ENOG4110DG4 LUCA
GeneTreeiENSGT00550000074425
HOGENOMiHOG000234630
HOVERGENiHBG054085
InParanoidiQ9ERZ8
KOiK04973
OMAiKVCNEDQ
OrthoDBiEOG091G016O
PhylomeDBiQ9ERZ8
TreeFamiTF314711

Enzyme and pathway databases

ReactomeiR-RNO-3295583 TRP channels

Miscellaneous databases

PROiPR:Q9ERZ8

Gene expression databases

BgeeiENSRNOG00000001195 Expressed in 9 organ(s), highest expression level in adult mammalian kidney
GenevisibleiQ9ERZ8 RN

Family and domain databases

CDDicd00204 ANK, 1 hit
Gene3Di1.25.40.20, 1 hit
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
IPR005821 Ion_trans_dom
IPR004729 TRP_channel
IPR024862 TRPV
IPR008347 TRPV1-4_channel
IPR008348 TRPV4_channel
PANTHERiPTHR10582 PTHR10582, 1 hit
PTHR10582:SF4 PTHR10582:SF4, 1 hit
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF00520 Ion_trans, 1 hit
PRINTSiPR01768 TRPVRECEPTOR
PR01769 VRL2RECEPTOR
SMARTiView protein in SMART
SM00248 ANK, 3 hits
SUPFAMiSSF48403 SSF48403, 1 hit
TIGRFAMsiTIGR00870 trp, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTRPV4_RAT
AccessioniPrimary (citable) accession number: Q9ERZ8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 26, 2005
Last sequence update: March 1, 2001
Last modified: September 12, 2018
This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
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Main funding by: National Institutes of Health

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