UniProtKB - Q9ERK4 (XPO2_MOUSE)
Protein
Exportin-2
Gene
Cse1l
Organism
Mus musculus (Mouse)
Status
Functioni
Export receptor for importin-alpha. Mediates importin-alpha re-export from the nucleus to the cytoplasm after import substrates (cargos) have been released into the nucleoplasm. In the nucleus binds cooperatively to importin-alpha and to the GTPase Ran in its active GTP-bound form. Docking of this trimeric complex to the nuclear pore complex (NPC) is mediated through binding to nucleoporins. Upon transit of a nuclear export complex into the cytoplasm, disassembling of the complex and hydrolysis of Ran-GTP to Ran-GDP (induced by RANBP1 and RANGAP1, respectively) cause release of the importin-alpha from the export receptor. CSE1L/XPO2 then return to the nuclear compartment and mediate another round of transport. The directionality of nuclear export is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus.By similarity
GO - Molecular functioni
- nuclear export signal receptor activity Source: GO_Central
- Ran GTPase binding Source: InterPro
GO - Biological processi
- protein export from nucleus Source: GO_Central
- protein import into nucleus Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Exportin-2Short name: Exp2 Alternative name(s): Chromosome segregation 1-like protein Importin-alpha re-exporter |
Gene namesi | Name:Cse1l Synonyms:Xpo2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1339951, Cse1l |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear envelope Source: GO_Central
- nucleoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
Complete embryonic lethality at around 5.5 dpc.1 Publication
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000117288 | 1 – 971 | Exportin-2Add BLAST | 971 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 112 | PhosphoserineBy similarity | 1 | |
Modified residuei | 574 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 824 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 931 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9ERK4 |
jPOSTi | Q9ERK4 |
MaxQBi | Q9ERK4 |
PaxDbi | Q9ERK4 |
PeptideAtlasi | Q9ERK4 |
PRIDEi | Q9ERK4 |
PTM databases
iPTMneti | Q9ERK4 |
PhosphoSitePlusi | Q9ERK4 |
SwissPalmi | Q9ERK4 |
Expressioni
Tissue specificityi
Ubiquitous. Detected in embryos from 5 to 17 dpc. Highly expressed in adult testis, heart, brain, lung, liver, skeletal muscle, spleen and kidney.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000002718, Expressed in forelimb bud and 321 other tissues |
ExpressionAtlasi | Q9ERK4, baseline and differential |
Genevisiblei | Q9ERK4, MM |
Interactioni
Subunit structurei
Found in a complex with CSE1L/XPO2, Ran and KPNA2. Binds with high affinity to importin-alpha only in the presence of RanGTP. The complex is dissociated by the combined action of RanBP1 and RanGAP1.
Interacts with CFTR.
By similarityGO - Molecular functioni
- Ran GTPase binding Source: InterPro
Protein-protein interaction databases
BioGRIDi | 225874, 34 interactors |
IntActi | Q9ERK4, 9 interactors |
MINTi | Q9ERK4 |
STRINGi | 10090.ENSMUSP00000002790 |
Miscellaneous databases
RNActi | Q9ERK4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 29 – 102 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 74 |
Sequence similaritiesi
Belongs to the XPO2/CSE1 family.Curated
Phylogenomic databases
eggNOGi | KOG1992, Eukaryota |
GeneTreei | ENSGT00550000074884 |
HOGENOMi | CLU_009614_0_0_1 |
InParanoidi | Q9ERK4 |
OMAi | MPYVFQM |
OrthoDBi | 1248958at2759 |
PhylomeDBi | Q9ERK4 |
TreeFami | TF300473 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR005043, XPO2_C IPR013713, XPO2_central |
Pfami | View protein in Pfam PF03378, CAS_CSE1, 1 hit PF08506, Cse1, 1 hit PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
Q9ERK4-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MELSDANLQT LTEYLKKTLD PDPAIRRPAE KFLESVEGNQ NYPLLLLTLL
60 70 80 90 100
EKSQDNVIKV CASVTFKNYI KRNWRIVEDE PNKICEADRV AIKANIVHLM
110 120 130 140 150
LSSPEQIQKQ LSDAISIIGR EDFPQKWPDL LTEMVNRFQS GDFHVINGVL
160 170 180 190 200
RTAHSLFKRY RHEFKSNELW TEIKLVLDAF ALPLTNLFKA TIELCSTHAN
210 220 230 240 250
DASALRILFS SLILISKLFY SLNFQDLPEF FEDNMETWMN NFHTLLTLDN
260 270 280 290 300
KLLQTDDEEE AGLLELLKSQ ICDNAALYAQ KYDEEFQRYL PRFVTAIWNL
310 320 330 340 350
LVTTGREVKY DLLVSNAIQF LASVCERPHY KNLFEDQNTL TSICEKVIVP
360 370 380 390 400
NMEFRAADEE AFEDNSEEYI RRDLEGSDID TRRRAACDLV RGLCKFFEGP
410 420 430 440 450
VTGIFSGYVN SMLQEYAKNP SVNWKHKDAA IYLVTSLASK AQTQKHGITQ
460 470 480 490 500
ANELVNLTEF FVNHILPDLK SNNVNEFPVL KADGIKYIMI FRNQVPKEHL
510 520 530 540 550
LVSIPLLISH LEAESIVVHT YAAHALERLF TMRGSNNTTL FTAAEIAPFV
560 570 580 590 600
EILLTNLFKA LTLPGSSENE YIMKAIMRSF SLLQEAIIPY IPTLITQLTQ
610 620 630 640 650
KLLAVSKNPS KPHFNHYMFE AICLSIRITC KANPAAVVNF EEALFLVFTE
660 670 680 690 700
ILQNDVQEFI PYVFQVMSLL LETHKNDIPS SYMALFPHLL QPVLWERTGN
710 720 730 740 750
IPALVRLLQA FLERGSSTIA TAAADKIPGL LGVFQKLIAS KANDHQGFYL
760 770 780 790 800
LNSIIEHMPP ESVDQYRKQI FILLFQRLQN SKTTKFIKSF LVFINLYCIK
810 820 830 840 850
YGALALQEIF DGIQPKMFGM VLEKIIIPEI QKVSGNVEKK ICAVGITKLL
860 870 880 890 900
TECPPMMDTE YTKLWTPLLQ SLIGLFELPE DDSIPDEEHF IDIEDTPGYQ
910 920 930 940 950
TAFSQLAFAG KKEHDPVGQM VNNPKIHLAQ SLHKLSTACP GRVPSMVSTS
960 970
LNAEALQYLQ GYLQAASVTL L
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketE9Q1T9 | E9Q1T9_MOUSE | Chromosome segregation 1-like prote... | Cse1l | 915 | Annotation score: | ||
E9QAX7 | E9QAX7_MOUSE | Exportin-2 | Cse1l | 415 | Annotation score: | ||
F6ZEW4 | F6ZEW4_MOUSE | Exportin-2 | Cse1l | 658 | Annotation score: | ||
F7D1H9 | F7D1H9_MOUSE | Exportin-2 | Cse1l | 74 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF301152 mRNA Translation: AAG24636.1 |
CCDSi | CCDS17092.1 |
RefSeqi | NP_076054.1, NM_023565.3 |
Genome annotation databases
Ensembli | ENSMUST00000002790; ENSMUSP00000002790; ENSMUSG00000002718 |
GeneIDi | 110750 |
KEGGi | mmu:110750 |
UCSCi | uc008nys.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF301152 mRNA Translation: AAG24636.1 |
CCDSi | CCDS17092.1 |
RefSeqi | NP_076054.1, NM_023565.3 |
3D structure databases
SMRi | Q9ERK4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225874, 34 interactors |
IntActi | Q9ERK4, 9 interactors |
MINTi | Q9ERK4 |
STRINGi | 10090.ENSMUSP00000002790 |
PTM databases
iPTMneti | Q9ERK4 |
PhosphoSitePlusi | Q9ERK4 |
SwissPalmi | Q9ERK4 |
Proteomic databases
EPDi | Q9ERK4 |
jPOSTi | Q9ERK4 |
MaxQBi | Q9ERK4 |
PaxDbi | Q9ERK4 |
PeptideAtlasi | Q9ERK4 |
PRIDEi | Q9ERK4 |
Protocols and materials databases
Antibodypediai | 28372, 510 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000002790; ENSMUSP00000002790; ENSMUSG00000002718 |
GeneIDi | 110750 |
KEGGi | mmu:110750 |
UCSCi | uc008nys.2, mouse |
Organism-specific databases
CTDi | 1434 |
MGIi | MGI:1339951, Cse1l |
Phylogenomic databases
eggNOGi | KOG1992, Eukaryota |
GeneTreei | ENSGT00550000074884 |
HOGENOMi | CLU_009614_0_0_1 |
InParanoidi | Q9ERK4 |
OMAi | MPYVFQM |
OrthoDBi | 1248958at2759 |
PhylomeDBi | Q9ERK4 |
TreeFami | TF300473 |
Miscellaneous databases
BioGRID-ORCSi | 110750, 11 hits in 17 CRISPR screens |
ChiTaRSi | Cse1l, mouse |
PROi | PR:Q9ERK4 |
RNActi | Q9ERK4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000002718, Expressed in forelimb bud and 321 other tissues |
ExpressionAtlasi | Q9ERK4, baseline and differential |
Genevisiblei | Q9ERK4, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR005043, XPO2_C IPR013713, XPO2_central |
Pfami | View protein in Pfam PF03378, CAS_CSE1, 1 hit PF08506, Cse1, 1 hit PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | XPO2_MOUSE | |
Accessioni | Q9ERK4Primary (citable) accession number: Q9ERK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 23, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families