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Protein

Ras-related protein Rab-27A

Gene

Rab27a

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in cytotoxic granule exocytosis in lymphocytes. Required for both granule maturation and granule docking and priming at the immunologic synapse (By similarity).By similarity

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi16 – 24GTP9
Nucleotide bindingi74 – 78GTP5
Nucleotide bindingi133 – 136GTP4
Nucleotide bindingi163 – 165GTP3

GO - Molecular functioni

  • GDP binding Source: UniProtKB
  • GTPase activity Source: UniProtKB
  • GTP binding Source: UniProtKB
  • myosin V binding Source: MGI
  • protein domain specific binding Source: MGI

GO - Biological processi

Keywordsi

Biological processExocytosis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Names & Taxonomyi

Protein namesi
Recommended name:
Ras-related protein Rab-27A
Gene namesi
Name:Rab27a
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 9

Organism-specific databases

MGIiMGI:1861441 Rab27a

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Endosome, Lysosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi78Q → L: Loss of GTPase activity. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00001212222 – 221Ras-related protein Rab-27AAdd BLAST220

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylserineBy similarity1
Modified residuei2PhosphoserineBy similarity1
Disulfide bondi123 ↔ 188By similarity
Lipidationi219S-geranylgeranyl cysteineBy similarity1
Modified residuei221Cysteine methyl esterBy similarity1
Lipidationi221S-geranylgeranyl cysteineBy similarity1

Keywords - PTMi

Acetylation, Disulfide bond, Lipoprotein, Methylation, Phosphoprotein, Prenylation

Proteomic databases

EPDiQ9ERI2
MaxQBiQ9ERI2
PaxDbiQ9ERI2
PRIDEiQ9ERI2

PTM databases

iPTMnetiQ9ERI2
PhosphoSitePlusiQ9ERI2

Expressioni

Tissue specificityi

Detected in melanocytes. Expressed abundantly in the stomach and is predominantly localized at the apical region of gastric-surface mucus cells. Also expressed in the thymus and lung.1 Publication

Gene expression databases

BgeeiENSMUSG00000032202 Expressed in 220 organ(s), highest expression level in dorsal pancreas
CleanExiMM_RAB27A
ExpressionAtlasiQ9ERI2 baseline and differential
GenevisibleiQ9ERI2 MM

Interactioni

Subunit structurei

Binds SYTL1, SYTL2, SLAC2B, MYRIP, SYTL3, SYTL4, SYTL5 and MLPH. Interacts with UNC13D. Interacts with RPH3A and RPH3A. Does not interact with the BLOC-3 complex (heterodimer of HPS1 and HPS4) (By similarity).By similarity8 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi198222, 6 interactors
CORUMiQ9ERI2
DIPiDIP-31054N
IntActiQ9ERI2, 15 interactors
STRINGi10090.ENSMUSP00000034722

Structurei

Secondary structure

1221
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9ERI2
SMRiQ9ERI2
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ERI2

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi38 – 46Effector regionBy similarity9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rab family.Curated

Phylogenomic databases

eggNOGiKOG0081 Eukaryota
ENOG410XQ3T LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ9ERI2
KOiK07885
OMAiLVMQRIQ
OrthoDBiEOG091G0I51
PhylomeDBiQ9ERI2
TreeFamiTF312895

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ERI2-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MSDGDYDYLI KFLALGDSGV GKTSVLYQYT DGKFNSKFIT TVGIDFREKR
60 70 80 90 100
VVYRANGPDG AVGRGQRIHL QLWDTAGQER FRSLTTAFFR DAMGFLLLFD
110 120 130 140 150
LTNEQSFLNV RNWISQLQMH AYCENPDIVL CGNKSDLEDQ RAVKEEEARE
160 170 180 190 200
LAEKYGIPYF ETSAANGTNI SHAIEMLLDL IMKRMERCVD KSWIPEGVVR
210 220
SNGHTSADQL SEEKEKGLCG C
Length:221
Mass (Da):25,017
Last modified:March 1, 2001 - v1
Checksum:iC6857EBDD7F38137
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304376 mRNA Translation: AAG24638.1
AB046693 mRNA Translation: BAB62313.1
AK010373 mRNA Translation: BAB26891.1
BC008173 mRNA Translation: AAH08173.1
BC009656 mRNA Translation: AAH09656.1
CCDSiCCDS23335.1
RefSeqiNP_001288159.1, NM_001301230.1
NP_001288161.1, NM_001301232.1
NP_076124.1, NM_023635.6
XP_006510843.1, XM_006510780.3
UniGeneiMm.34867
Mm.490032

Genome annotation databases

EnsembliENSMUST00000034722; ENSMUSP00000034722; ENSMUSG00000032202
ENSMUST00000184146; ENSMUSP00000139310; ENSMUSG00000032202
GeneIDi11891
KEGGimmu:11891
UCSCiuc009qqv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF304376 mRNA Translation: AAG24638.1
AB046693 mRNA Translation: BAB62313.1
AK010373 mRNA Translation: BAB26891.1
BC008173 mRNA Translation: AAH08173.1
BC009656 mRNA Translation: AAH09656.1
CCDSiCCDS23335.1
RefSeqiNP_001288159.1, NM_001301230.1
NP_001288161.1, NM_001301232.1
NP_076124.1, NM_023635.6
XP_006510843.1, XM_006510780.3
UniGeneiMm.34867
Mm.490032

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3BC1X-ray1.80A/E1-193[»]
ProteinModelPortaliQ9ERI2
SMRiQ9ERI2
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi198222, 6 interactors
CORUMiQ9ERI2
DIPiDIP-31054N
IntActiQ9ERI2, 15 interactors
STRINGi10090.ENSMUSP00000034722

PTM databases

iPTMnetiQ9ERI2
PhosphoSitePlusiQ9ERI2

Proteomic databases

EPDiQ9ERI2
MaxQBiQ9ERI2
PaxDbiQ9ERI2
PRIDEiQ9ERI2

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000034722; ENSMUSP00000034722; ENSMUSG00000032202
ENSMUST00000184146; ENSMUSP00000139310; ENSMUSG00000032202
GeneIDi11891
KEGGimmu:11891
UCSCiuc009qqv.2 mouse

Organism-specific databases

CTDi5873
MGIiMGI:1861441 Rab27a

Phylogenomic databases

eggNOGiKOG0081 Eukaryota
ENOG410XQ3T LUCA
GeneTreeiENSGT00890000139330
HOGENOMiHOG000233968
HOVERGENiHBG009351
InParanoidiQ9ERI2
KOiK07885
OMAiLVMQRIQ
OrthoDBiEOG091G0I51
PhylomeDBiQ9ERI2
TreeFamiTF312895

Enzyme and pathway databases

ReactomeiR-MMU-6798695 Neutrophil degranulation
R-MMU-8873719 RAB geranylgeranylation
R-MMU-8876198 RAB GEFs exchange GTP for GDP on RABs

Miscellaneous databases

ChiTaRSiRab27a mouse
EvolutionaryTraceiQ9ERI2
PROiPR:Q9ERI2
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000032202 Expressed in 220 organ(s), highest expression level in dorsal pancreas
CleanExiMM_RAB27A
ExpressionAtlasiQ9ERI2 baseline and differential
GenevisibleiQ9ERI2 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51419 RAB, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRB27A_MOUSE
AccessioniPrimary (citable) accession number: Q9ERI2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: April 16, 2002
Last sequence update: March 1, 2001
Last modified: September 12, 2018
This is version 160 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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