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Protein

LIM domain and actin-binding protein 1

Gene

Lima1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Actin-binding protein involved in actin cytoskeleton regulation and dynamics. Increases the number and size of actin stress fibers and inhibits membrane ruffling. Inhibits actin filament depolymerization. Bundles actin filaments, delays filament nucleation and reduces formation of branched filaments (By similarity). Plays a role in cholesterol homeostasis. Influences plasma cholesterol levels through regulation of intestinal cholesterol absorption. May act as a scaffold protein by regulating NPC1L1 transportation, an essential protein for cholesterol absorption, to the plasma membrane by recruiting MYO5B to NPC1L1, and thus facilitates cholesterol uptake (PubMed:29880681).By similarity1 Publication

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionActin-binding
Biological processCholesterol metabolism, Lipid metabolism, Steroid metabolism, Sterol metabolism
LigandMetal-binding, Zinc

Names & Taxonomyi

Protein namesi
Recommended name:
LIM domain and actin-binding protein 1
Alternative name(s):
Epithelial protein lost in neoplasm
Short name:
mEPLIN
Gene namesi
Name:Lima1
Synonyms:D15Ertd366e, Eplin1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1920992 Lima1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Cell projection, Cytoplasm, Cytoskeleton, Membrane

Pathology & Biotechi

Disruption phenotypei

Deficient mice display reduced dietary cholesterol absorption.1 Publication

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi164 – 166CLG → AAA: Decreases interaction with NPC1L1. 1 Publication3
Mutagenesisi360S → A: Abolished phosphorylation by MAPK1/MAPK3. 1 Publication1
Mutagenesisi602S → A: Reduced phosphorylation by MAPK1/MAPK3. 1 Publication1
Mutagenesisi692S → A: Reduced phosphorylation by MAPK1/MAPK3. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000757311 – 753LIM domain and actin-binding protein 1Add BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei15PhosphoserineBy similarity1
Modified residuei132PhosphoserineCombined sources1
Modified residuei225PhosphoserineCombined sources1
Modified residuei230PhosphoserineCombined sources1
Modified residuei242PhosphoserineCombined sources1
Modified residuei263PhosphoserineBy similarity1
Modified residuei348PhosphoserineBy similarity1
Modified residuei360PhosphoserineCombined sources1 Publication1
Modified residuei367PhosphoserineBy similarity1
Modified residuei372PhosphoserineBy similarity1
Modified residuei437N6-succinyllysineCombined sources1
Modified residuei467PhosphoserineCombined sources1
Modified residuei485PhosphoserineCombined sources1
Modified residuei488PhosphoserineCombined sources1
Modified residuei599PhosphoserineBy similarity1
Modified residuei602Phosphoserine1 Publication1
Modified residuei607PhosphoserineBy similarity1
Modified residuei615PhosphoserineCombined sources1
Modified residuei692Phosphoserine1 Publication1
Modified residuei720PhosphoserineCombined sources1
Modified residuei735PhosphoserineCombined sources1

Post-translational modificationi

Phosphorylation of the C-terminal region by MAPK1/MAPK3 reduces its association with F-actin and contributes to actin filament reorganization and enhances cell motility.1 Publication

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ERG0
PeptideAtlasiQ9ERG0
PRIDEiQ9ERG0

PTM databases

iPTMnetiQ9ERG0
PhosphoSitePlusiQ9ERG0
SwissPalmiQ9ERG0

Expressioni

Tissue specificityi

Highly expressed in the small intestine, including the duodenum, jejunum, and ileum. Low expression in the liver and very low expressed in the heart, spleen, lung, brain, and pancreas (PubMed:29880681). Isoform Alpha is highly expressed in embryos from day 7-11 and in adult spleen and lung. Isoform Beta expression is highest in adult kidney, testis, lung and liver, intermediate in heart, brain, spleen, skeletal muscle and low in embryos.1 Publication

Gene expression databases

BgeeiENSMUSG00000023022 Expressed in 288 organ(s), highest expression level in colon
CleanExiMM_LIMA1
ExpressionAtlasiQ9ERG0 baseline and differential
GenevisibleiQ9ERG0 MM

Interactioni

Subunit structurei

Interacts with NPC1L1; bridges NPC1L1 with MYO5B (PubMed:29880681). Interacts with MYO5B; bridges MYO5B with NPC1L1 (PubMed:29880681). Interacts with PXN; this complex stabilizes actin dynamics (By similarity). Binds to G-actin and F-actin (PubMed:17875928) (By similarity).By similarity2 Publications

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211164, 133 interactors
DIPiDIP-29634N
ELMiQ9ERG0
IntActiQ9ERG0, 139 interactors
MINTiQ9ERG0
STRINGi10090.ENSMUSP00000073371

Structurei

3D structure databases

ProteinModelPortaliQ9ERG0
SMRiQ9ERG0
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini386 – 446LIM zinc-bindingPROSITE-ProRule annotationAdd BLAST61

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni491 – 511Required for interaction with MYO5B1 PublicationAdd BLAST21

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi164 – 166Required for interaction with NPC1L11 Publication3

Domaini

Keywords - Domaini

LIM domain

Phylogenomic databases

eggNOGiENOG410ITI7 Eukaryota
ENOG41102B0 LUCA
GeneTreeiENSGT00510000046839
HOGENOMiHOG000059621
HOVERGENiHBG051492
InParanoidiQ9ERG0
OMAiPKWPPED
OrthoDBiEOG091G0043
TreeFamiTF350273

Family and domain databases

InterProiView protein in InterPro
IPR028740 EPLIN
IPR001781 Znf_LIM
PANTHERiPTHR24206:SF50 PTHR24206:SF50, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 1 hit
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative promoter usage. AlignAdd to basket

This entry has 2 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All

Isoform Beta (identifier: Q9ERG0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MESTPFNRRQ WTSLSLRVTA KELSLVNKNK SSAIVEIFSK YQKAAEEANM
60 70 80 90 100
ERKKNNPESL PQHFRRGTLS VLKKKWENPV AGAEFHTDSL PNSSSEGGHT
110 120 130 140 150
ADYPPAEVTD KPAPGVRADR EEHTQPKPRF GSRPEAVIQS RYPRSENSHD
160 170 180 190 200
FKAQATESQK MENCLGDSRH EAEKPETSEN TETSGKIEKY NVPLNRLKMM
210 220 230 240 250
FEKGEHNQTK SLWTQSRNAG GRRLSENNCS LDDWEIGAGH LSSSAFNSEK
260 270 280 290 300
NESKRNLELP RLSETSIKDR MAKYQAAVSK QSSPASYTNE LKTSESKTHK
310 320 330 340 350
WEQKENVPPG PEACSVHQEG SKVSTTENSL VALSVPAEDD TCNSQVKSEA
360 370 380 390 400
QQPMHPKPLS PDARTSSLPE SSPSKTAKKF QAPAKESCVE CQKTVYPMER
410 420 430 440 450
LLANQQVFHI SCFRCSYCNN KLSLGTYASL HGRIYCKPHF NQLFKSKGNY
460 470 480 490 500
DEGFGHKQHK DLWASKSDNE ETLGRPAQPP NAGESPHSPG VEDAPIAKVG
510 520 530 540 550
VLAASMEAKA SSQREREDKP AETKKLRIAW PPPAELGGSG SALEEGIKVS
560 570 580 590 600
KPKWPPEDDV CKTEAPEDVD LDLKKLRRSS SLKERSRPFT VAASFRTSSI
610 620 630 640 650
KSPKASSPSL RKGWSESEQS EEFGGGIATM ERKQTENARP SGEKENVGKS
660 670 680 690 700
RWQGEEVPRS KDRSSFELES ENFMENGANI AEDDNHVHAQ QSPLEPEAPG
710 720 730 740 750
WSGFVDTTAA KEFTTQNQKS QDVGFWEGEV VRELSVEEQI KRNRYYDEDE

DEE
Length:753
Mass (Da):84,060
Last modified:July 27, 2011 - v3
Checksum:i79F98E47C100CF22
GO
Isoform Alpha (identifier: Q9ERG0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-160: Missing.

Show »
Length:593
Mass (Da):66,018
Checksum:i122AB8AA07E1FC0A
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2R8VI67A0A2R8VI67_MOUSE
LIM domain and actin-binding protei...
Lima1
109Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti216S → N in AAG31148 (PubMed:11179679).Curated1
Sequence conflicti325T → A in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti486P → S in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti499V → A in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti538G → S in AAG31147 (PubMed:11179679).Curated1
Sequence conflicti605A → T in AAG31147 (PubMed:11179679).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0031181 – 160Missing in isoform Alpha. 1 PublicationAdd BLAST160

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307844 mRNA Translation: AAG31147.1
AF307845 mRNA Translation: AAG31148.1
BC031490 mRNA Translation: AAH31490.1
CH466550 Genomic DNA Translation: EDL04110.1
CCDSiCCDS37206.1 [Q9ERG0-2]
CCDS49729.1 [Q9ERG0-1]
RefSeqiNP_001107017.1, NM_001113545.1 [Q9ERG0-1]
UniGeneiMm.33207

Genome annotation databases

EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022 [Q9ERG0-1]
ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022 [Q9ERG0-2]
GeneIDi65970
KEGGimmu:65970
UCSCiuc007xqh.3 mouse [Q9ERG0-1]

Keywords - Coding sequence diversityi

Alternative promoter usage

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF307844 mRNA Translation: AAG31147.1
AF307845 mRNA Translation: AAG31148.1
BC031490 mRNA Translation: AAH31490.1
CH466550 Genomic DNA Translation: EDL04110.1
CCDSiCCDS37206.1 [Q9ERG0-2]
CCDS49729.1 [Q9ERG0-1]
RefSeqiNP_001107017.1, NM_001113545.1 [Q9ERG0-1]
UniGeneiMm.33207

3D structure databases

ProteinModelPortaliQ9ERG0
SMRiQ9ERG0
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211164, 133 interactors
DIPiDIP-29634N
ELMiQ9ERG0
IntActiQ9ERG0, 139 interactors
MINTiQ9ERG0
STRINGi10090.ENSMUSP00000073371

PTM databases

iPTMnetiQ9ERG0
PhosphoSitePlusiQ9ERG0
SwissPalmiQ9ERG0

Proteomic databases

PaxDbiQ9ERG0
PeptideAtlasiQ9ERG0
PRIDEiQ9ERG0

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000073691; ENSMUSP00000073371; ENSMUSG00000023022 [Q9ERG0-1]
ENSMUST00000109024; ENSMUSP00000104652; ENSMUSG00000023022 [Q9ERG0-2]
GeneIDi65970
KEGGimmu:65970
UCSCiuc007xqh.3 mouse [Q9ERG0-1]

Organism-specific databases

CTDi51474
MGIiMGI:1920992 Lima1

Phylogenomic databases

eggNOGiENOG410ITI7 Eukaryota
ENOG41102B0 LUCA
GeneTreeiENSGT00510000046839
HOGENOMiHOG000059621
HOVERGENiHBG051492
InParanoidiQ9ERG0
OMAiPKWPPED
OrthoDBiEOG091G0043
TreeFamiTF350273

Miscellaneous databases

ChiTaRSiLima1 mouse
PROiPR:Q9ERG0
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000023022 Expressed in 288 organ(s), highest expression level in colon
CleanExiMM_LIMA1
ExpressionAtlasiQ9ERG0 baseline and differential
GenevisibleiQ9ERG0 MM

Family and domain databases

InterProiView protein in InterPro
IPR028740 EPLIN
IPR001781 Znf_LIM
PANTHERiPTHR24206:SF50 PTHR24206:SF50, 1 hit
PfamiView protein in Pfam
PF00412 LIM, 1 hit
SMARTiView protein in SMART
SM00132 LIM, 1 hit
PROSITEiView protein in PROSITE
PS00478 LIM_DOMAIN_1, 1 hit
PS50023 LIM_DOMAIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiLIMA1_MOUSE
AccessioniPrimary (citable) accession number: Q9ERG0
Secondary accession number(s): Q8K2H0, Q9ERG1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 5, 2002
Last sequence update: July 27, 2011
Last modified: October 10, 2018
This is version 133 of the entry and version 3 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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