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Protein

Neuroglobin

Gene

Ngb

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Involved in oxygen transport in the brain. Hexacoordinate globin, displaying competitive binding of oxygen or the distal His residue to the iron atom. Not capable of penetrating cell membranes. The deoxygenated form exhibits nitrite reductase activity inhibiting cellular respiration via NO-binding to cytochrome c oxidase. Involved in neuroprotection during oxidative stress. May exert its anti-apoptotic activity by acting to reset the trigger level of mitochondrial cytochrome c release necessary to commit the cells to apoptosis.2 Publications

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi64Iron (heme distal ligand)Combined sources1 Publication1
Metal bindingi96Iron (heme proximal ligand)Combined sources2 Publications1

GO - Molecular functioni

  • heme binding Source: InterPro
  • metal ion binding Source: UniProtKB-KW
  • oxygen binding Source: InterPro
  • oxygen carrier activity Source: MGI

GO - Biological processi

Keywordsi

Biological processApoptosis, Oxygen transport, Transport
LigandHeme, Iron, Metal-binding

Enzyme and pathway databases

ReactomeiR-MMU-8981607 Intracellular oxygen transport

Names & Taxonomyi

Protein namesi
Recommended name:
Neuroglobin
Gene namesi
Name:Ngb
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:2151886 Ngb

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi64H → L: Improved binding of dioxygen and carbon monoxide to the iron atom. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000533921 – 151NeuroglobinAdd BLAST151

Post-translational modificationi

A redox disulfide bond regulates the heme pocket coordination and the rate of nitrite reduction to NO.By similarity
Phosphorylated in vitro by ERK1, ERK2 and PKA, and in vivo during hypoxia. Phosphorylation increases nitrite reductase activity (By similarity).By similarity

Keywords - PTMi

Disulfide bond, Phosphoprotein

Proteomic databases

PaxDbiQ9ER97
PRIDEiQ9ER97

Expressioni

Tissue specificityi

Predominantly expressed in brain.

Gene expression databases

BgeeiENSMUSG00000021032 Expressed in 94 organ(s), highest expression level in heart left ventricle
CleanExiMM_NGB
ExpressionAtlasiQ9ER97 baseline and differential
GenevisibleiQ9ER97 MM

Interactioni

Subunit structurei

Monomer. Homodimer and homotetramer; disulfide-linked (Probable). Interacts with 14-3-3 (By similarity).By similarityCurated

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021420

Structurei

Secondary structure

1151
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9ER97
SMRiQ9ER97
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9ER97

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 149GlobinAdd BLAST149

Sequence similaritiesi

Belongs to the globin family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG3378 Eukaryota
COG1018 LUCA
GeneTreeiENSGT00510000048375
HOVERGENiHBG039321
InParanoidiQ9ER97
KOiK21893
PhylomeDBiQ9ER97
TreeFamiTF333247

Family and domain databases

Gene3Di1.10.490.10, 1 hit
InterProiView protein in InterPro
IPR000971 Globin
IPR009050 Globin-like_sf
IPR012292 Globin/Proto
PfamiView protein in Pfam
PF00042 Globin, 1 hit
SUPFAMiSSF46458 SSF46458, 1 hit
PROSITEiView protein in PROSITE
PS01033 GLOBIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All

Q9ER97-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MERPESELIR QSWRVVSRSP LEHGTVLFAR LFALEPSLLP LFQYNGRQFS
60 70 80 90 100
SPEDCLSSPE FLDHIRKVML VIDAAVTNVE DLSSLEEYLT SLGRKHRAVG
110 120 130 140 150
VRLSSFSTVG ESLLYMLEKC LGPDFTPATR TAWSRLYGAV VQAMSRGWDG

E
Length:151
Mass (Da):17,037
Last modified:March 1, 2001 - v1
Checksum:i377BBE4BF723CCF1
GO

Computationally mapped potential isoform sequencesi

There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q3USR6Q3USR6_MOUSE
Neuroglobin
Ngb
155Annotation score:
D3YW07D3YW07_MOUSE
Neuroglobin
Ngb
92Annotation score:

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245945 mRNA Translation: CAC11135.1
BC024263 mRNA Translation: AAH24263.1
CCDSiCCDS36501.1
RefSeqiNP_071859.1, NM_022414.2
UniGeneiMm.41395

Genome annotation databases

EnsembliENSMUST00000021420; ENSMUSP00000021420; ENSMUSG00000021032
GeneIDi64242
KEGGimmu:64242
UCSCiuc007oii.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ245945 mRNA Translation: CAC11135.1
BC024263 mRNA Translation: AAH24263.1
CCDSiCCDS36501.1
RefSeqiNP_071859.1, NM_022414.2
UniGeneiMm.41395

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q1FX-ray1.50A1-151[»]
1W92X-ray1.70A1-151[»]
2VRYX-ray1.87A1-151[»]
3GK9X-ray1.80A1-151[»]
3GKTX-ray1.86A1-151[»]
3GLNX-ray2.26A1-151[»]
4MU5X-ray1.80A1-151[»]
4NZIX-ray2.10A1-151[»]
4O1TX-ray1.60A1-151[»]
4O2GX-ray2.70A1-151[»]
4O35X-ray1.80A1-151[»]
4O4TX-ray1.90A1-151[»]
4O4ZX-ray1.70A1-151[»]
5EETX-ray2.00A3-150[»]
5EOHX-ray1.90A3-150[»]
5EQMX-ray2.05A3-150[»]
5EU2X-ray2.00A3-150[»]
5EV5X-ray2.00A3-150[»]
5EYJX-ray2.40A3-150[»]
5EYSX-ray1.75A3-150[»]
5F0BX-ray2.15A3-151[»]
5F2AX-ray2.10A1-151[»]
5MJCX-ray1.62A3-150[»]
5MJDX-ray1.70A3-150[»]
5NVIX-ray1.60A3-150[»]
5NW6X-ray1.70A3-150[»]
5O17X-ray1.80A3-150[»]
5O18X-ray1.86A3-150[»]
5O1KX-ray2.05A3-150[»]
5O27X-ray2.31A3-150[»]
6EYEX-ray1.70A1-151[»]
ProteinModelPortaliQ9ER97
SMRiQ9ER97
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi10090.ENSMUSP00000021420

Proteomic databases

PaxDbiQ9ER97
PRIDEiQ9ER97

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000021420; ENSMUSP00000021420; ENSMUSG00000021032
GeneIDi64242
KEGGimmu:64242
UCSCiuc007oii.1 mouse

Organism-specific databases

CTDi58157
MGIiMGI:2151886 Ngb

Phylogenomic databases

eggNOGiKOG3378 Eukaryota
COG1018 LUCA
GeneTreeiENSGT00510000048375
HOVERGENiHBG039321
InParanoidiQ9ER97
KOiK21893
PhylomeDBiQ9ER97
TreeFamiTF333247

Enzyme and pathway databases

ReactomeiR-MMU-8981607 Intracellular oxygen transport

Miscellaneous databases

EvolutionaryTraceiQ9ER97
PROiPR:Q9ER97
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000021032 Expressed in 94 organ(s), highest expression level in heart left ventricle
CleanExiMM_NGB
ExpressionAtlasiQ9ER97 baseline and differential
GenevisibleiQ9ER97 MM

Family and domain databases

Gene3Di1.10.490.10, 1 hit
InterProiView protein in InterPro
IPR000971 Globin
IPR009050 Globin-like_sf
IPR012292 Globin/Proto
PfamiView protein in Pfam
PF00042 Globin, 1 hit
SUPFAMiSSF46458 SSF46458, 1 hit
PROSITEiView protein in PROSITE
PS01033 GLOBIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNGB_MOUSE
AccessioniPrimary (citable) accession number: Q9ER97
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 20, 2003
Last sequence update: March 1, 2001
Last modified: September 12, 2018
This is version 118 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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