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Protein

Rho-related GTP-binding protein RhoJ

Gene

Rhoj

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plasma membrane-associated small GTPase specifically involved in angiogenesis (By similarity). Required for endothelial cell migration during vascular development via its interaction with GLUL (By similarity). Elicits the formation of F-actin-rich structures, thereby regulating endothelial cell migration (PubMed:10967094).By similarity1 Publication

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi31 – 36GTPBy similarity6
Nucleotide bindingi46 – 53GTPBy similarity8
Nucleotide bindingi75 – 79GTPBy similarity5
Nucleotide bindingi133 – 136GTPBy similarity4
Nucleotide bindingi177 – 178GTPBy similarity2

GO - Molecular functioni

  • GTPase activity Source: MGI
  • GTP binding Source: GO_Central
  • protein kinase binding Source: GO_Central

GO - Biological processi

Keywordsi

Biological processAngiogenesis
LigandGTP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Names & Taxonomyi

Protein namesi
Recommended name:
Rho-related GTP-binding protein RhoJ
Alternative name(s):
Tc10-like GTP-binding protein1 Publication
Gene namesi
Name:Rhoj
Synonyms:Arhj, Rhoi1 Publication, Rhot, Tc10l, Tcl1 Publication
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 12

Organism-specific databases

MGIiMGI:1931551 Rhoj

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001988701 – 211Rho-related GTP-binding protein RhoJAdd BLAST211
PropeptideiPRO_0000281221212 – 214Removed in mature formCurated3

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Lipidationi3S-palmitoyl cysteineBy similarity1
Lipidationi11S-palmitoyl cysteineBy similarity1
Modified residuei211Cysteine methyl esterBy similarity1
Lipidationi211S-farnesyl cysteineBy similarity1

Post-translational modificationi

Palmitoylated; regulates localization to the plasma membrane and may be mediated by GLUL.By similarity

Keywords - PTMi

Lipoprotein, Methylation, Palmitate, Prenylation

Proteomic databases

PaxDbiQ9ER71
PRIDEiQ9ER71

PTM databases

iPTMnetiQ9ER71
PhosphoSitePlusiQ9ER71

Expressioni

Tissue specificityi

Highly expressed in heart with moderate levels in lung and liver (PubMed:10967094). Very low levels detected in brain, spleen, skeletal muscle, kidney and testis (PubMed:10967094).1 Publication

Gene expression databases

BgeeiENSMUSG00000046768 Expressed in 252 organ(s), highest expression level in brain blood vessel
CleanExiMM_RHOJ
ExpressionAtlasiQ9ER71 baseline and differential
GenevisibleiQ9ER71 MM

Interactioni

Subunit structurei

Interacts with the CRIB domains of proteins such as Pak1 and Was/Wasp (PubMed:10967094). Interacts with GLUL (By similarity).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

BioGridi219817, 6 interactors
STRINGi10090.ENSMUSP00000059498

Structurei

3D structure databases

ProteinModelPortaliQ9ER71
SMRiQ9ER71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi50 – 58Effector regionSequence analysis9

Sequence similaritiesi

Belongs to the small GTPase superfamily. Rho family.Curated

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9ER71
KOiK07864
OMAiKKRCSEC
OrthoDBiEOG091G0KCM
PhylomeDBiQ9ER71
TreeFamiTF101109

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit

Sequences (2+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

This entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All

Isoform 1 (identifier: Q9ER71-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSCRERTDSS CGCNGHEENR ILKCVVVGDG AVGKTCLLMS YANDAFPEEY
60 70 80 90 100
VPTVFDHYAV TVTVGGKQHL LGLYDTAGQE DYNQLRPLSY PNTDVFLICF
110 120 130 140 150
SVVNPASYHN VQEEWVPELK DCMPHVPYVL IGTQIDLRDD PKTLARLLYM
160 170 180 190 200
KEKPLTYEHG VKLAKAIGAQ CYLECSALTQ KGLKAVFDEA ILTIFHPKKK
210
KKGCLGCHGC CAII
Length:214
Mass (Da):23,766
Last modified:October 25, 2002 - v2
Checksum:iA8070C73583A8AA3
GO
Isoform 2 (identifier: Q9ER71-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     4-13: Missing.

Note: No experimental confirmation available.
Show »
Length:204
Mass (Da):22,671
Checksum:i542A5E1AC93CF25F
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
D3YX61D3YX61_MOUSE
Rho-related GTP-binding protein Rho...
Rhoj
171Annotation score:
F2Z463F2Z463_MOUSE
Rho-related GTP-binding protein Rho...
Rhoj
167Annotation score:
G3UZM2G3UZM2_MOUSE
Rho-related GTP-binding protein Rho...
Rhoj
96Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti166A → R in AAL09441 (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0057094 – 13Missing in isoform 2. 1 Publication10

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276568 mRNA Translation: CAC06700.1
AB060651 mRNA Translation: BAB91069.1
AF309564 mRNA Translation: AAL09441.1
AK003482 mRNA Translation: BAB22812.1
AK003490 mRNA Translation: BAB22818.1
AK156619 mRNA Translation: BAE33778.1
AK159385 mRNA Translation: BAE35040.1
BC043719 mRNA Translation: AAH43719.1
CCDSiCCDS25981.1 [Q9ER71-1]
RefSeqiNP_075764.1, NM_023275.2 [Q9ER71-1]
UniGeneiMm.27467

Genome annotation databases

EnsembliENSMUST00000055390; ENSMUSP00000059498; ENSMUSG00000046768 [Q9ER71-1]
GeneIDi80837
KEGGimmu:80837
UCSCiuc007nxc.1 mouse [Q9ER71-1]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ276568 mRNA Translation: CAC06700.1
AB060651 mRNA Translation: BAB91069.1
AF309564 mRNA Translation: AAL09441.1
AK003482 mRNA Translation: BAB22812.1
AK003490 mRNA Translation: BAB22818.1
AK156619 mRNA Translation: BAE33778.1
AK159385 mRNA Translation: BAE35040.1
BC043719 mRNA Translation: AAH43719.1
CCDSiCCDS25981.1 [Q9ER71-1]
RefSeqiNP_075764.1, NM_023275.2 [Q9ER71-1]
UniGeneiMm.27467

3D structure databases

ProteinModelPortaliQ9ER71
SMRiQ9ER71
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219817, 6 interactors
STRINGi10090.ENSMUSP00000059498

PTM databases

iPTMnetiQ9ER71
PhosphoSitePlusiQ9ER71

Proteomic databases

PaxDbiQ9ER71
PRIDEiQ9ER71

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000055390; ENSMUSP00000059498; ENSMUSG00000046768 [Q9ER71-1]
GeneIDi80837
KEGGimmu:80837
UCSCiuc007nxc.1 mouse [Q9ER71-1]

Organism-specific databases

CTDi57381
MGIiMGI:1931551 Rhoj

Phylogenomic databases

eggNOGiKOG0393 Eukaryota
COG1100 LUCA
GeneTreeiENSGT00760000118978
HOGENOMiHOG000233974
HOVERGENiHBG009351
InParanoidiQ9ER71
KOiK07864
OMAiKKRCSEC
OrthoDBiEOG091G0KCM
PhylomeDBiQ9ER71
TreeFamiTF101109

Enzyme and pathway databases

ReactomeiR-MMU-194840 Rho GTPase cycle

Miscellaneous databases

PROiPR:Q9ER71
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000046768 Expressed in 252 organ(s), highest expression level in brain blood vessel
CleanExiMM_RHOJ
ExpressionAtlasiQ9ER71 baseline and differential
GenevisibleiQ9ER71 MM

Family and domain databases

InterProiView protein in InterPro
IPR027417 P-loop_NTPase
IPR005225 Small_GTP-bd_dom
IPR001806 Small_GTPase
IPR003578 Small_GTPase_Rho
PfamiView protein in Pfam
PF00071 Ras, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
TIGRFAMsiTIGR00231 small_GTP, 1 hit
PROSITEiView protein in PROSITE
PS51420 RHO, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiRHOJ_MOUSE
AccessioniPrimary (citable) accession number: Q9ER71
Secondary accession number(s): Q3TX76, Q920E4, Q9CQA7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 25, 2002
Last sequence update: October 25, 2002
Last modified: November 7, 2018
This is version 139 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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