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Protein

Aconitate hydratase, mitochondrial

Gene

Aco2

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the isomerization of citrate to isocitrate via cis-aconitate.By similarity

Catalytic activityi

Citrate = isocitrate.By similarity

Cofactori

[4Fe-4S] clusterBy similarityNote: Binds 1 [4Fe-4S] cluster per subunit. Binding of a [3Fe-4S] cluster leads to an inactive enzyme.By similarity

Pathwayi: tricarboxylic acid cycle

This protein is involved in step 2 of the subpathway that synthesizes isocitrate from oxaloacetate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Citrate synthase, mitochondrial (Cs)
  2. Aconitate hydratase, mitochondrial (Aco2)
This subpathway is part of the pathway tricarboxylic acid cycle, which is itself part of Carbohydrate metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isocitrate from oxaloacetate, the pathway tricarboxylic acid cycle and in Carbohydrate metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei99SubstrateBy similarity1
Metal bindingi385Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi448Iron-sulfur (4Fe-4S)By similarity1
Metal bindingi451Iron-sulfur (4Fe-4S)By similarity1
Binding sitei474SubstrateBy similarity1
Binding sitei479SubstrateBy similarity1
Binding sitei607SubstrateBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLyase
Biological processTricarboxylic acid cycle
Ligand4Fe-4S, Iron, Iron-sulfur, Metal-binding

Enzyme and pathway databases

BRENDAi4.2.1.3 5301
ReactomeiR-RNO-71403 Citric acid cycle (TCA cycle)
UniPathwayi
UPA00223;UER00718

Names & Taxonomyi

Protein namesi
Recommended name:
Aconitate hydratase, mitochondrial (EC:4.2.1.3By similarity)
Short name:
Aconitase
Alternative name(s):
Citrate hydro-lyase
Gene namesi
Name:Aco2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 7

Organism-specific databases

RGDi621360 Aco2

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Mitochondrion

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 27MitochondrionBy similarityAdd BLAST27
ChainiPRO_000000054428 – 780Aconitate hydratase, mitochondrialAdd BLAST753

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei31N6-succinyllysineBy similarity1
Modified residuei50N6-acetyllysine; alternateBy similarity1
Modified residuei50N6-succinyllysine; alternateBy similarity1
Modified residuei138N6-acetyllysine; alternateBy similarity1
Modified residuei138N6-succinyllysine; alternateBy similarity1
Modified residuei144N6-acetyllysine; alternateBy similarity1
Modified residuei144N6-succinyllysine; alternateBy similarity1
Modified residuei233N6-acetyllysine; alternateBy similarity1
Modified residuei233N6-succinyllysine; alternateBy similarity1
Modified residuei411N6-succinyllysineBy similarity1
Modified residuei517N6-acetyllysine; alternateBy similarity1
Modified residuei517N6-succinyllysine; alternateBy similarity1
Modified residuei523N6-acetyllysine; alternateBy similarity1
Modified residuei523N6-succinyllysine; alternateBy similarity1
Modified residuei549N6-succinyllysineBy similarity1
Modified residuei559PhosphoserineCombined sources1
Modified residuei573N6-acetyllysine; alternateBy similarity1
Modified residuei573N6-succinyllysine; alternateBy similarity1
Modified residuei577N6-succinyllysineBy similarity1
Modified residuei591N6-succinyllysineBy similarity1
Modified residuei605N6-acetyllysine; alternateBy similarity1
Modified residuei605N6-succinyllysine; alternateBy similarity1
Modified residuei628N6-succinyllysineBy similarity1
Modified residuei670PhosphoserineBy similarity1
Modified residuei689N6-succinyllysineBy similarity1
Modified residuei723N6-acetyllysine; alternateBy similarity1
Modified residuei723N6-succinyllysine; alternateBy similarity1
Modified residuei730N6-acetyllysine; alternateBy similarity1
Modified residuei730N6-succinyllysine; alternateBy similarity1
Modified residuei736N6-acetyllysineBy similarity1
Modified residuei743N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PaxDbiQ9ER34
PRIDEiQ9ER34

2D gel databases

World-2DPAGEi0004:Q9ER34

PTM databases

CarbonylDBiQ9ER34
iPTMnetiQ9ER34
PhosphoSitePlusiQ9ER34
SwissPalmiQ9ER34

Expressioni

Gene expression databases

BgeeiENSRNOG00000024128 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiQ9ER34 RN

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi249448, 3 interactors
IntActiQ9ER34, 1 interactor
STRINGi10116.ENSRNOP00000029144

Structurei

3D structure databases

ProteinModelPortaliQ9ER34
SMRiQ9ER34
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni192 – 194Substrate bindingBy similarity3
Regioni670 – 671Substrate bindingBy similarity2

Sequence similaritiesi

Belongs to the aconitase/IPM isomerase family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0453 Eukaryota
COG1048 LUCA
GeneTreeiENSGT00930000150926
HOGENOMiHOG000224293
HOVERGENiHBG000248
InParanoidiQ9ER34
KOiK01681
OMAiGCIGMGQ
OrthoDBiEOG091G024R
PhylomeDBiQ9ER34
TreeFamiTF300627

Family and domain databases

Gene3Di3.20.19.10, 1 hit
3.30.499.10, 2 hits
3.40.1060.10, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR015932 Aconitase_dom2
IPR006248 Aconitase_mito-like
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01340 aconitase_mito, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9ER34-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MAPYSLLVTR LQKALGVRQY HVASALCQRA KVAMSHFEPS EYIRYDLLEK
60 70 80 90 100
NINIVRKRLN RPLTLSEKIV YGHLDDPANQ EIERGKTYLR LRPDRVAMQD
110 120 130 140 150
ATAQMAMLQF ISSGLPKVAV PSTIHCDHLI EAQLGGEKDL RRAKDINQEV
160 170 180 190 200
YNFLATAGAK YGVGFWRPGS GIIHQIILEN YAYPGVLLIG TDSHTPNGGG
210 220 230 240 250
LGGICIGVGG ADAVDVMAGI PWELKCPKVI GVKLTGTLSG WTSPKDVILK
260 270 280 290 300
VAGILTVKGG TGAIVEYHGP GVDSISCTGM ATICNMGAEI GATTSVFPYN
310 320 330 340 350
HRMKKYLSKT GRADIANLAE EFKDHLVPDP GCQYDQVIEI NLNELKPHIN
360 370 380 390 400
GPFTPDLAHP VADVGTVAEK EGWPLDIRVG LIGSCTNSSY EDMGRSAAVA
410 420 430 440 450
KQALAHGLKC KSQFTITPGS EQIRATIERD GYAQILRDVG GIVLANACGP
460 470 480 490 500
CIGQWDRKDI KKGEKNTIVT SYNRNFTGRN DANPETHAFV TSPEIVTALA
510 520 530 540 550
IAGTLKFNPE TDFLTGKDGK KFKLEAPDAD ELPRSDFDPG QDTYQHPPKD
560 570 580 590 600
SSGQRVDVSP TSQRLQLLEP FDKWDGKDLE DLQILIKVKG KCTTDHISAA
610 620 630 640 650
GPWLKFRGHL DNISNNLLIG AINIENGKAN SVRNAVTQEF GPVPDTARYY
660 670 680 690 700
KKHGIRWVVI GDENYGEGSS REHAALEPRH LGGRAIITKS FARIHETNLK
710 720 730 740 750
KQGLLPLTFA DPSDYNKIHP VDKLTIQGLK DFAPGKPLNC IIKHPNGTQE
760 770 780
TILLNHTFNE TQIEWFRAGS ALNRMKELQQ
Length:780
Mass (Da):85,433
Last modified:February 1, 2005 - v2
Checksum:iE523564299DD38BD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti224L → P in CAC11018 (Ref. 1) Curated1
Sequence conflicti295S → P in CAC11018 (Ref. 1) Curated1
Sequence conflicti582L → Q in CAC11018 (Ref. 1) Curated1
Sequence conflicti659V → M in CAC11018 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243266 mRNA Translation: CAC11018.1
BC061999 mRNA Translation: AAH61999.1
RefSeqiNP_077374.2, NM_024398.2
UniGeneiRn.43737

Genome annotation databases

EnsembliENSRNOT00000038612; ENSRNOP00000029144; ENSRNOG00000024128
GeneIDi79250
KEGGirno:79250
UCSCiRGD:621360 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ243266 mRNA Translation: CAC11018.1
BC061999 mRNA Translation: AAH61999.1
RefSeqiNP_077374.2, NM_024398.2
UniGeneiRn.43737

3D structure databases

ProteinModelPortaliQ9ER34
SMRiQ9ER34
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi249448, 3 interactors
IntActiQ9ER34, 1 interactor
STRINGi10116.ENSRNOP00000029144

PTM databases

CarbonylDBiQ9ER34
iPTMnetiQ9ER34
PhosphoSitePlusiQ9ER34
SwissPalmiQ9ER34

2D gel databases

World-2DPAGEi0004:Q9ER34

Proteomic databases

PaxDbiQ9ER34
PRIDEiQ9ER34

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000038612; ENSRNOP00000029144; ENSRNOG00000024128
GeneIDi79250
KEGGirno:79250
UCSCiRGD:621360 rat

Organism-specific databases

CTDi50
RGDi621360 Aco2

Phylogenomic databases

eggNOGiKOG0453 Eukaryota
COG1048 LUCA
GeneTreeiENSGT00930000150926
HOGENOMiHOG000224293
HOVERGENiHBG000248
InParanoidiQ9ER34
KOiK01681
OMAiGCIGMGQ
OrthoDBiEOG091G024R
PhylomeDBiQ9ER34
TreeFamiTF300627

Enzyme and pathway databases

UniPathwayi
UPA00223;UER00718

BRENDAi4.2.1.3 5301
ReactomeiR-RNO-71403 Citric acid cycle (TCA cycle)

Miscellaneous databases

PROiPR:Q9ER34

Gene expression databases

BgeeiENSRNOG00000024128 Expressed in 10 organ(s), highest expression level in heart
GenevisibleiQ9ER34 RN

Family and domain databases

Gene3Di3.20.19.10, 1 hit
3.30.499.10, 2 hits
3.40.1060.10, 1 hit
InterProiView protein in InterPro
IPR015931 Acnase/IPM_dHydase_lsu_aba_1/3
IPR001030 Acoase/IPM_deHydtase_lsu_aba
IPR015928 Aconitase/3IPM_dehydase_swvl
IPR018136 Aconitase_4Fe-4S_BS
IPR036008 Aconitase_4Fe-4S_dom
IPR015932 Aconitase_dom2
IPR006248 Aconitase_mito-like
IPR000573 AconitaseA/IPMdHydase_ssu_swvl
PfamiView protein in Pfam
PF00330 Aconitase, 1 hit
PF00694 Aconitase_C, 1 hit
PRINTSiPR00415 ACONITASE
SUPFAMiSSF53732 SSF53732, 1 hit
TIGRFAMsiTIGR01340 aconitase_mito, 1 hit
PROSITEiView protein in PROSITE
PS00450 ACONITASE_1, 1 hit
PS01244 ACONITASE_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiACON_RAT
AccessioniPrimary (citable) accession number: Q9ER34
Secondary accession number(s): Q6P6V3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: February 1, 2005
Last sequence update: February 1, 2005
Last modified: November 7, 2018
This is version 129 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
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Main funding by: National Institutes of Health

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