UniProtKB - Q9ER00 (STX12_MOUSE)
Protein
Syntaxin-12
Gene
Stx12
Organism
Mus musculus (Mouse)
Status
Functioni
SNARE that acts to regulate protein transport between late endosomes and the trans-Golgi network. The SNARE complex containing STX6, STX12, VAMP4 and VTI1A mediates vesicle fusion (in vitro) (By similarity). Through complex formation with GRIP1, GRIA2 and NSG1 controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).By similarity
GO - Molecular functioni
- SNAP receptor activity Source: GO_Central
- SNARE binding Source: GO_Central
GO - Biological processi
- autophagosome assembly Source: MGI
- cholesterol efflux Source: MGI
- intracellular protein transport Source: GO_Central
- protein stabilization Source: MGI
- synaptic vesicle to endosome fusion Source: MGI
- vesicle docking Source: GO_Central
- vesicle fusion Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Syntaxin-12 |
Gene namesi | Name:Stx12 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1931027, Stx12 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus membrane ; Single-pass type IV membrane protein By similarity
Endosome
- Endosome membrane ; Single-pass type IV membrane protein By similarity
- Early endosome membrane By similarity; Single-pass type IV membrane protein Curated
- Recycling endosome membrane By similarity; Single-pass type IV membrane protein By similarity
Other locations
- Endomembrane system Curated; Single-pass type IV membrane protein Curated; Cytoplasmic side Curated
Cytosol
- BLOC-1 complex Source: Ensembl
Endosome
- early endosome membrane Source: UniProtKB-SubCell
- postsynaptic recycling endosome Source: MGI
- recycling endosome membrane Source: UniProtKB-SubCell
Golgi apparatus
- Golgi membrane Source: UniProtKB-SubCell
Other locations
- endomembrane system Source: GO_Central
- integral component of membrane Source: GO_Central
- integral component of synaptic vesicle membrane Source: MGI
- membrane raft Source: MGI
- phagocytic vesicle Source: MGI
- phagophore assembly site Source: MGI
- SNARE complex Source: UniProtKB
- synaptic vesicle Source: MGI
- vesicle Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 2 – 250 | CytoplasmicSequence analysisAdd BLAST | 249 | |
Transmembranei | 251 – 271 | Helical; Anchor for type IV membrane proteinSequence analysisAdd BLAST | 21 | |
Topological domaini | 272 – 274 | VesicularSequence analysis | 3 |
Keywords - Cellular componenti
Endosome, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000210224 | 2 – 274 | Syntaxin-12Add BLAST | 273 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylserineBy similarity | 1 | |
Modified residuei | 139 | PhosphoserineCombined sources | 1 | |
Modified residuei | 142 | PhosphoserineCombined sources | 1 | |
Modified residuei | 218 | PhosphoserineCombined sources | 1 | |
Modified residuei | 225 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9ER00 |
jPOSTi | Q9ER00 |
MaxQBi | Q9ER00 |
PaxDbi | Q9ER00 |
PeptideAtlasi | Q9ER00 |
PRIDEi | Q9ER00 |
PTM databases
iPTMneti | Q9ER00 |
PhosphoSitePlusi | Q9ER00 |
SwissPalmi | Q9ER00 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000028879, Expressed in Ammon's horn and 305 other tissues |
Genevisiblei | Q9ER00, MM |
Interactioni
Subunit structurei
Associates with the BLOC-1 complex.
Interacts with BLOC1S6.
Interacts with NAPA and SNAP23.
Identified in a complex containing STX6, STX12, VAMP4 and VTI1A (By similarity).
Interacts with GRIPAP1 (PubMed:20098723).
Forms a complex with GRIP1, GRIA2 and NSG1; controls the intracellular fate of AMPAR and the endosomal sorting of the GRIA2 subunit toward recycling and membrane targeting (By similarity).
Interacts with NSG1 (PubMed:12070131).
By similarity2 PublicationsGO - Molecular functioni
- SNARE binding Source: GO_Central
Protein-protein interaction databases
BioGRIDi | 221409, 16 interactors |
IntActi | Q9ER00, 8 interactors |
MINTi | Q9ER00 |
STRINGi | 10090.ENSMUSP00000030698 |
Miscellaneous databases
RNActi | Q9ER00, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 178 – 240 | t-SNARE coiled-coil homologyPROSITE-ProRule annotationAdd BLAST | 63 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 33 – 130 | Sequence analysisAdd BLAST | 98 |
Sequence similaritiesi
Belongs to the syntaxin family.Curated
Keywords - Domaini
Coiled coil, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0811, Eukaryota |
GeneTreei | ENSGT01000000214440 |
HOGENOMi | CLU_059257_1_1_1 |
InParanoidi | Q9ER00 |
OMAi | HQIQHYT |
OrthoDBi | 1204812at2759 |
PhylomeDBi | Q9ER00 |
TreeFami | TF315607 |
Family and domain databases
CDDi | cd00179, SynN, 1 hit |
InterProi | View protein in InterPro IPR010989, SNARE IPR006012, Syntaxin/epimorphin_CS IPR006011, Syntaxin_N IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF05739, SNARE, 1 hit PF14523, Syntaxin_2, 1 hit |
SMARTi | View protein in SMART SM00503, SynN, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF47661, SSF47661, 1 hit |
PROSITEi | View protein in PROSITE PS00914, SYNTAXIN, 1 hit PS50192, T_SNARE, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9ER00-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MSYGPLDMYR NPGPSGPQPR DFNSIIQTCS GNIQRISQAT AQIKNLMSQL
60 70 80 90 100
GTKQDSSKLQ ENLQQLQHST NQLAKETNEL LKELGSLPLP LSASEQRQQK
110 120 130 140 150
LQKERLMNDF SSALNNFQVV QRKVSEKEKE SIARARAGSR LSAEDRQREE
160 170 180 190 200
QLVSFDSHEE WNQMQSQEEE AAITEQDLEL IKERETAIRQ LEADILDVNQ
210 220 230 240 250
IFKDLAMMIH DQGDLIDSIE ANVESSEVHV ERATDQLQRA AYYQKKSRKK
260 270
MCILVLVLSV IVTVLVVVIW VASK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 171 | A → V in AAH10669 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019211 mRNA Translation: BAB20282.1 AK077678 mRNA Translation: BAC36951.1 AK078948 mRNA Translation: BAC37473.1 AK146544 mRNA Translation: BAE27249.1 AK146735 mRNA Translation: BAE27397.1 AK167087 mRNA Translation: BAE39244.1 BC010669 mRNA Translation: AAH10669.1 |
CCDSi | CCDS18737.1 |
RefSeqi | NP_598648.1, NM_133887.4 |
Genome annotation databases
Ensembli | ENSMUST00000030698; ENSMUSP00000030698; ENSMUSG00000028879 |
GeneIDi | 100226 |
KEGGi | mmu:100226 |
UCSCi | uc008vbx.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB019211 mRNA Translation: BAB20282.1 AK077678 mRNA Translation: BAC36951.1 AK078948 mRNA Translation: BAC37473.1 AK146544 mRNA Translation: BAE27249.1 AK146735 mRNA Translation: BAE27397.1 AK167087 mRNA Translation: BAE39244.1 BC010669 mRNA Translation: AAH10669.1 |
CCDSi | CCDS18737.1 |
RefSeqi | NP_598648.1, NM_133887.4 |
3D structure databases
SMRi | Q9ER00 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 221409, 16 interactors |
IntActi | Q9ER00, 8 interactors |
MINTi | Q9ER00 |
STRINGi | 10090.ENSMUSP00000030698 |
PTM databases
iPTMneti | Q9ER00 |
PhosphoSitePlusi | Q9ER00 |
SwissPalmi | Q9ER00 |
Proteomic databases
EPDi | Q9ER00 |
jPOSTi | Q9ER00 |
MaxQBi | Q9ER00 |
PaxDbi | Q9ER00 |
PeptideAtlasi | Q9ER00 |
PRIDEi | Q9ER00 |
Protocols and materials databases
Antibodypediai | 30837, 171 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000030698; ENSMUSP00000030698; ENSMUSG00000028879 |
GeneIDi | 100226 |
KEGGi | mmu:100226 |
UCSCi | uc008vbx.1, mouse |
Organism-specific databases
CTDi | 23673 |
MGIi | MGI:1931027, Stx12 |
Phylogenomic databases
eggNOGi | KOG0811, Eukaryota |
GeneTreei | ENSGT01000000214440 |
HOGENOMi | CLU_059257_1_1_1 |
InParanoidi | Q9ER00 |
OMAi | HQIQHYT |
OrthoDBi | 1204812at2759 |
PhylomeDBi | Q9ER00 |
TreeFami | TF315607 |
Miscellaneous databases
BioGRID-ORCSi | 100226, 1 hit in 17 CRISPR screens |
ChiTaRSi | Stx12, mouse |
PROi | PR:Q9ER00 |
RNActi | Q9ER00, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000028879, Expressed in Ammon's horn and 305 other tissues |
Genevisiblei | Q9ER00, MM |
Family and domain databases
CDDi | cd00179, SynN, 1 hit |
InterProi | View protein in InterPro IPR010989, SNARE IPR006012, Syntaxin/epimorphin_CS IPR006011, Syntaxin_N IPR000727, T_SNARE_dom |
Pfami | View protein in Pfam PF05739, SNARE, 1 hit PF14523, Syntaxin_2, 1 hit |
SMARTi | View protein in SMART SM00503, SynN, 1 hit SM00397, t_SNARE, 1 hit |
SUPFAMi | SSF47661, SSF47661, 1 hit |
PROSITEi | View protein in PROSITE PS00914, SYNTAXIN, 1 hit PS50192, T_SNARE, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | STX12_MOUSE | |
Accessioni | Q9ER00Primary (citable) accession number: Q9ER00 Secondary accession number(s): Q3UIV9, Q921T9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families