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Protein

Prostaglandin reductase 1

Gene

Ptgr1

Organism
Cavia porcellus (Guinea pig)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Functions as 15-oxo-prostaglandin 13-reductase and acts on 15-oxo-PGE1, 15-oxo-PGE2 and 15-oxo-PGE2-alpha. Catalyzes the conversion of leukotriene B4 into its biologically less active metabolite, 12-oxo-leukotriene B4. This is an initial and key step of metabolic inactivation of leukotriene B4.1 Publication

Catalytic activityi

A n-alkanal + NAD(P)+ = an alk-2-enal + NAD(P)H.
11-alpha-hydroxy-9,15-dioxoprost-5-enoate + NAD(P)+ = (5Z)-(13E)-11-alpha-hydroxy-9,15-dioxoprosta-5,13-dienoate + NAD(P)H.

Kineticsi

  1. KM=93 µM for LTB4
  2. KM=35 µM for 15-keto-PGE2
  1. Vmax=1.7 nmol/min/mg enzyme with LTB4 as substrate
  2. Vmax=345 nmol/min/mg enzyme with 15-keto-PGE2 as substrate

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei178NADP1 Publication1
Binding sitei193NADP1 Publication1
Binding sitei217NADP1 Publication1
Binding sitei321NADP1 Publication1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi152 – 155NADP1 Publication4
Nucleotide bindingi239 – 245NADP1 Publication7
Nucleotide bindingi270 – 272NADP1 Publication3

GO - Molecular functioni

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Names & Taxonomyi

Protein namesi
Recommended name:
Prostaglandin reductase 1 (EC:1.3.1.-)
Short name:
PRG-1
Alternative name(s):
15-oxoprostaglandin 13-reductase (EC:1.3.1.48)
Short name:
PGR
NADP-dependent leukotriene B4 12-hydroxydehydrogenase (EC:1.3.1.74)
Short name:
12-HD
Short name:
LTB4
Gene namesi
Name:Ptgr1
Synonyms:Ltb4dh
OrganismiCavia porcellus (Guinea pig)
Taxonomic identifieri10141 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaHystricomorphaCaviidaeCavia
Proteomesi
  • UP000005447 Componenti: Unassembled WGS sequence

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002180641 – 329Prostaglandin reductase 1Add BLAST329

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei18PhosphothreonineBy similarity1
Modified residuei20PhosphoserineBy similarity1
Modified residuei178N6-acetyllysineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

PRIDEiQ9EQZ5

Expressioni

Tissue specificityi

Detected in small intestine, kidney, liver, spleen and stomach (at protein level). Detected in small intestine, kidney and liver.1 Publication

Interactioni

Subunit structurei

Monomer or homodimer.1 Publication

Structurei

Secondary structure

1329
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details

3D structure databases

ProteinModelPortaliQ9EQZ5
SMRiQ9EQZ5
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiQ9EQZ5

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

HOVERGENiHBG055024
InParanoidiQ9EQZ5

Family and domain databases

CDDicd08294 leukotriene_B4_DH_like, 1 hit
InterProiView protein in InterPro
IPR013149 ADH_C
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
IPR014190 PTGR1
PfamiView protein in Pfam
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02825 B4_12hDH, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EQZ5-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MVKAKSWTLK KHFQGKPTQS DFELKTVELP PLKNGEVLLE ALFLSVDPYM
60 70 80 90 100
RIASKRLKEG AVMMGQQVAR VVESKNSAFP AGSIVLAQSG WTTHFISDGK
110 120 130 140 150
GLEKLLTEWP DKLPLSLALG TIGMPGLTAY FGLLEVCGVK GGETVLVSAA
160 170 180 190 200
AGAVGSVVGQ IAKLKGCKVV GAAGSDEKIA YLKQIGFDAA FNYKTVNSLE
210 220 230 240 250
EALKKASPDG YDCYFDNVGG EFLNTVLSQM KDFGKIAICG AISVYNRMDQ
260 270 280 290 300
LPPGPSPESI IYKQLRIEGF IVYRWQGDVR EKALRDLMKW VLEGKIQYHE
310 320
HVTKGFENMP AAFIEMLNGA NLGKAVVTA
Length:329
Mass (Da):35,730
Last modified:March 1, 2001 - v1
Checksum:i8C5D86D9D2C9FEA1
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021219 mRNA Translation: BAB20289.1
RefSeqiNP_001166451.1, NM_001172980.1

Genome annotation databases

GeneIDi100135572

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB021219 mRNA Translation: BAB20289.1
RefSeqiNP_001166451.1, NM_001172980.1

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1V3TX-ray2.30A/B1-329[»]
1V3UX-ray2.00A/B1-329[»]
1V3VX-ray2.00A/B1-329[»]
2DM6X-ray2.00A/B1-329[»]
ProteinModelPortaliQ9EQZ5
SMRiQ9EQZ5
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiQ9EQZ5

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi100135572

Organism-specific databases

CTDi22949

Phylogenomic databases

HOVERGENiHBG055024
InParanoidiQ9EQZ5

Miscellaneous databases

EvolutionaryTraceiQ9EQZ5

Family and domain databases

CDDicd08294 leukotriene_B4_DH_like, 1 hit
InterProiView protein in InterPro
IPR013149 ADH_C
IPR011032 GroES-like_sf
IPR036291 NAD(P)-bd_dom_sf
IPR020843 PKS_ER
IPR014190 PTGR1
PfamiView protein in Pfam
PF00107 ADH_zinc_N, 1 hit
SMARTiView protein in SMART
SM00829 PKS_ER, 1 hit
SUPFAMiSSF50129 SSF50129, 2 hits
SSF51735 SSF51735, 1 hit
TIGRFAMsiTIGR02825 B4_12hDH, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiPTGR1_CAVPO
AccessioniPrimary (citable) accession number: Q9EQZ5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 16, 2005
Last sequence update: March 1, 2001
Last modified: December 20, 2017
This is version 96 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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