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Protein

Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4

Gene

Ndst4

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Essential bifunctional enzyme that catalyzes both the N-deacetylation and the N-sulfation of glucosamine (GlcNAc) of the glycosaminoglycan in heparan sulfate. Modifies the GlcNAc-GlcA disaccharide repeating sugar backbone to make N-sulfated heparosan, a prerequisite substrate for later modifications in heparin biosynthesis. Has low deacetylase activity but high sulfotransferase activity.1 Publication

Miscellaneous

The presence of 4 different heparan sulfate N-deacetylase/N-sulfotransferase enzymes in mammals, as well as differences in their enzyme activity suggest that some initiate heparan sulfate modification/sulfation reactions, whereas other later on fill in or extend already modified heparan sulfate sequences.

Catalytic activityi

3'-phosphoadenylyl sulfate + [heparan sulfate]-glucosamine = adenosine 3',5'-bisphosphate + [heparan sulfate]-N-sulfoglucosamine.

Pathwayi: heparan sulfate biosynthesis

This protein is involved in the pathway heparan sulfate biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparan sulfate biosynthesis and in Glycan metabolism.

Pathwayi: heparin biosynthesis

This protein is involved in the pathway heparin biosynthesis, which is part of Glycan metabolism.
View all proteins of this organism that are known to be involved in the pathway heparin biosynthesis and in Glycan metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei604For sulfotransferase activityBy similarity1
Binding sitei702PAPSBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi604 – 608PAPSBy similarity5
Nucleotide bindingi823 – 827PAPSBy similarity5

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Multifunctional enzyme, Transferase

Enzyme and pathway databases

BRENDAi2.8.2.8 3474
ReactomeiR-MMU-2022928 HS-GAG biosynthesis
UniPathwayi
UPA00756

UPA00862

Names & Taxonomyi

Protein namesi
Recommended name:
Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4 (EC:2.8.2.8)
Alternative name(s):
Glucosaminyl N-deacetylase/N-sulfotransferase 4
Short name:
NDST-4
N-heparan sulfate sulfotransferase 4
Short name:
N-HSST 4
Including the following 2 domains:
Heparan sulfate N-deacetylase 4 (EC:3.-.-.-)
Heparan sulfate N-sulfotransferase 4 (EC:2.8.2.-)
Gene namesi
Name:Ndst4
Synonyms:Hsst4
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 3

Organism-specific databases

MGIiMGI:1932545 Ndst4

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini1 – 13CytoplasmicSequence analysisAdd BLAST13
Transmembranei14 – 34Helical; Signal-anchor for type II membrane proteinSequence analysisAdd BLAST21
Topological domaini35 – 872LumenalSequence analysisAdd BLAST838

Keywords - Cellular componenti

Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002256621 – 872Bifunctional heparan sulfate N-deacetylase/N-sulfotransferase 4Add BLAST872

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi226N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi341N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi391N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi657N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi793N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi808 ↔ 818By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiQ9EQW8
PaxDbiQ9EQW8
PRIDEiQ9EQW8

PTM databases

PhosphoSitePlusiQ9EQW8

Expressioni

Tissue specificityi

Expressed at low level in brain and throughout embryogenesis. Not expressed in other tissues.1 Publication

Gene expression databases

BgeeiENSMUSG00000027971 Expressed in 48 organ(s), highest expression level in testis
ExpressionAtlasiQ9EQW8 baseline and differential
GenevisibleiQ9EQW8 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

BioGridi211087, 1 interactor
STRINGi10090.ENSMUSP00000133341

Structurei

3D structure databases

ProteinModelPortaliQ9EQW8
SMRiQ9EQW8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni36 – 588Heparan sulfate N-deacetylase 4Add BLAST553
Regioni589 – 872Heparan sulfate N-sulfotransferase 4Add BLAST284

Sequence similaritiesi

Keywords - Domaini

Signal-anchor, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG3703 Eukaryota
ENOG410XQN4 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000008010
HOVERGENiHBG082011
InParanoidiQ9EQW8
KOiK02579
OrthoDBiEOG091G02CP
PhylomeDBiQ9EQW8
TreeFamiTF313193

Family and domain databases

InterProiView protein in InterPro
IPR021930 Heparan_SO4_deacetylase
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF12062 HSNSD, 1 hit
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All

Q9EQW8-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MNLILKFRRS FRTLIVLLAT FCLVSILISA YFLYSGYKQE MTLIETTAEA
60 70 80 90 100
ECADIKDLPY RSIELRTIKP IDTSKTDPTV LLFVESQYSQ LGQDIIAILE
110 120 130 140 150
SSRFQYQMVI APGKGDIPPL TDSGKGKYTL IIYENILKYV SMDSWNRELL
160 170 180 190 200
EKYCIEYSVS IIGFHKANEN SLPTTQLKGF PLNLFNNVAL KDCSVNPQSP
210 220 230 240 250
LLHITKGPKV EKGPLPGEDW TIFQYNHSTY QPVLLTELQS EKSLSFLSSQ
260 270 280 290 300
TLYATIIQDL GLHDGIQRVL FGNNLNFWLH KLIFIDAISF LSGKRLTLSL
310 320 330 340 350
DRYILVDIDD IFVGKEGTRM NVKDVKALLE TQNLLRTQVA NFTFNLGFSG
360 370 380 390 400
KFYHTGTEEE DEGDDLLLRS VDEFWWFPHM WSHMQPHLFH NESSLVEQMI
410 420 430 440 450
LNKEFALEHG IPINLGYAVA PHHSGVYPVH IQLYAAWKKV WGIQVTSTEE
460 470 480 490 500
YPHLKPARYR KGFIHNSIMV LPRQTCGLFT HTIFYKEYPG GPQELDKSIK
510 520 530 540 550
GGELFLTILL NPISIFMTHL SNYGNDRLGL YTFVNLANFV HSWTNLKLQT
560 570 580 590 600
LPPVQLAHKY FELFPEQKDP LWQNPCDDKR HKDIWSREKT CDHLPKFLVI
610 620 630 640 650
GPQKTGTTAL YLFLLMHPSI ISNLPSPKTF EEVQFFNGNN YHKGIEWYMD
660 670 680 690 700
FFPTPSNITS DFLFEKSANY FHSEEAPKRA ASLVPKAKII TILIDPSDRA
710 720 730 740 750
YSWYQHQRSH EDPAALRFNF YEVITTGHWA PPDLKTLQRR CLVPGWYAVH
760 770 780 790 800
IERWLAYFST SQLLIIDGQQ LRSDPATVMD EVQKFLGVTP HYNYSEALTF
810 820 830 840 850
DPQKGFWCQL LEGGKTKCLG KSKGRKYPPM DSESRTFLSS YYRDHNVELS
860 870
KLLHRLGQPL PSWLRQELQK VR
Length:872
Mass (Da):100,655
Last modified:July 27, 2011 - v2
Checksum:iC56980F256C757E4
GO

Computationally mapped potential isoform sequencesi

There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
G3UX70G3UX70_MOUSE
Sulfotransferase
Ndst4
364Annotation score:
A0A0G2JDL4A0A0G2JDL4_MOUSE
Sulfotransferase
Ndst4
157Annotation score:
D3YWZ6D3YWZ6_MOUSE
Bifunctional heparan sulfate N-deac...
Ndst4
205Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti681A → T in BAB18517 (PubMed:11087757).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036838 mRNA Translation: BAB18517.1
AC111141 Genomic DNA No translation available.
AC140797 Genomic DNA No translation available.
AC164153 Genomic DNA No translation available.
CCDSiCCDS17820.1
RefSeqiNP_072087.2, NM_022565.2
XP_017175172.1, XM_017319683.1
UniGeneiMm.444289
Mm.483253

Genome annotation databases

EnsembliENSMUST00000173932; ENSMUSP00000133341; ENSMUSG00000027971
GeneIDi64580
KEGGimmu:64580
UCSCiuc008rfv.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB036838 mRNA Translation: BAB18517.1
AC111141 Genomic DNA No translation available.
AC140797 Genomic DNA No translation available.
AC164153 Genomic DNA No translation available.
CCDSiCCDS17820.1
RefSeqiNP_072087.2, NM_022565.2
XP_017175172.1, XM_017319683.1
UniGeneiMm.444289
Mm.483253

3D structure databases

ProteinModelPortaliQ9EQW8
SMRiQ9EQW8
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211087, 1 interactor
STRINGi10090.ENSMUSP00000133341

PTM databases

PhosphoSitePlusiQ9EQW8

Proteomic databases

MaxQBiQ9EQW8
PaxDbiQ9EQW8
PRIDEiQ9EQW8

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000173932; ENSMUSP00000133341; ENSMUSG00000027971
GeneIDi64580
KEGGimmu:64580
UCSCiuc008rfv.2 mouse

Organism-specific databases

CTDi64579
MGIiMGI:1932545 Ndst4

Phylogenomic databases

eggNOGiKOG3703 Eukaryota
ENOG410XQN4 LUCA
GeneTreeiENSGT00760000119023
HOGENOMiHOG000008010
HOVERGENiHBG082011
InParanoidiQ9EQW8
KOiK02579
OrthoDBiEOG091G02CP
PhylomeDBiQ9EQW8
TreeFamiTF313193

Enzyme and pathway databases

UniPathwayi
UPA00756

UPA00862

BRENDAi2.8.2.8 3474
ReactomeiR-MMU-2022928 HS-GAG biosynthesis

Miscellaneous databases

PROiPR:Q9EQW8
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000027971 Expressed in 48 organ(s), highest expression level in testis
ExpressionAtlasiQ9EQW8 baseline and differential
GenevisibleiQ9EQW8 MM

Family and domain databases

InterProiView protein in InterPro
IPR021930 Heparan_SO4_deacetylase
IPR037359 NST/OST
IPR027417 P-loop_NTPase
IPR000863 Sulfotransferase_dom
PANTHERiPTHR10605 PTHR10605, 1 hit
PfamiView protein in Pfam
PF12062 HSNSD, 1 hit
PF00685 Sulfotransfer_1, 1 hit
SUPFAMiSSF52540 SSF52540, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiNDST4_MOUSE
AccessioniPrimary (citable) accession number: Q9EQW8
Secondary accession number(s): D3Z1R7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 7, 2006
Last sequence update: July 27, 2011
Last modified: November 7, 2018
This is version 110 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. PATHWAY comments
    Index of metabolic and biosynthesis pathways
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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