UniProtKB - Q9EQW6 (OLIG2_MOUSE)
Protein
Oligodendrocyte transcription factor 2
Gene
Olig2
Organism
Mus musculus (Mouse)
Status
Functioni
Required for oligodendrocyte and motor neuron specification in the spinal cord, as well as for the development of somatic motor neurons in the hindbrain (PubMed:11955448, PubMed:12121626, PubMed:16908628). Functions together with ZNF488 to promote oligodendrocyte differentiation (PubMed:16908628). Cooperates with OLIG1 to establish the pMN domain of the embryonic neural tube (PubMed:11955448, PubMed:12121626). Antagonist of V2 interneuron and of NKX2-2-induced V3 interneuron development (PubMed:11955448, PubMed:12121626).3 Publications
GO - Molecular functioni
- DNA-binding transcription factor activity Source: MGI
- DNA-binding transcription factor activity, RNA polymerase II-specific Source: MGI
- HMG box domain binding Source: UniProtKB
- identical protein binding Source: MGI
- protein dimerization activity Source: InterPro
- RNA polymerase II cis-regulatory region sequence-specific DNA binding Source: GO_Central
- sequence-specific double-stranded DNA binding Source: MGI
GO - Biological processi
- myelination Source: MGI
- negative regulation of neuron differentiation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- nervous system development Source: MGI
- neuron differentiation Source: GO_Central
- neuron fate commitment Source: MGI
- oligodendrocyte differentiation Source: MGI
- positive regulation of oligodendrocyte differentiation Source: MGI
- regulation of transcription, DNA-templated Source: MGI
- regulation of transcription by RNA polymerase II Source: GO_Central
- spinal cord motor neuron differentiation Source: MGI
- spinal cord oligodendrocyte cell differentiation Source: MGI
- spinal cord oligodendrocyte cell fate specification Source: MGI
- thalamus development Source: MGI
Keywordsi
Molecular function | Developmental protein, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: Oligodendrocyte transcription factor 2Short name: Oligo2 |
Gene namesi | Name:Olig2 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1355331, Olig2 |
Subcellular locationi
Nucleus
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- transcription regulator complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000127415 | 1 – 323 | Oligodendrocyte transcription factor 2Add BLAST | 323 |
Proteomic databases
PaxDbi | Q9EQW6 |
PRIDEi | Q9EQW6 |
PTM databases
iPTMneti | Q9EQW6 |
PhosphoSitePlusi | Q9EQW6 |
Expressioni
Tissue specificityi
Expressed specifically in the brain.1 Publication
Developmental stagei
Expressed in the ventral spinal cord as early as 9.5 dpc. Expression becomes progressively restricted to a narrow zone within the ventral neuroepithelium of the spinal cord. In the 14.5 dpc spinal cord, expressed in the oligodendrocyte progenitors of the ventral ventricular zone, but not dorsal root ganglia Schwann cells. Also expressed scattered in the mantle zone, likely corresponding to oligodendrocyte progenitors migrating out from their site of origin. In the brain, from 10.5 through 14.5 dpc, expressed in numerous cells in the ventricular and subventricular zones of the lateral and medial ganglionic eminences, suggesting that expression might not be limited to the oligodendrocytic lineage. By 15.5 dpc, dispersed throughout the gray matter, with little or no residual expression in the ventricular zone. In the postnatal brain, present preferentially in the white matter, such as corpus callosum and cerebellar medulla. Expressed in the 13.5 and 14.5 dpc retina and in the olfactory epithelium from 11.5 dpc onward.2 Publications
Inductioni
By SHH. Also induced by NKX6-1 in the developing spinal cord, but not in the rostral hindbrain.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000039830, Expressed in lumbar subsegment of spinal cord and 123 other tissues |
ExpressionAtlasi | Q9EQW6, baseline and differential |
Genevisiblei | Q9EQW6, MM |
Interactioni
Subunit structurei
Binary interactionsi
Hide detailsGO - Molecular functioni
- HMG box domain binding Source: UniProtKB
- identical protein binding Source: MGI
- protein dimerization activity Source: InterPro
Protein-protein interaction databases
BioGRIDi | 206152, 8 interactors |
IntActi | Q9EQW6, 4 interactors |
STRINGi | 10090.ENSMUSP00000036797 |
Miscellaneous databases
RNActi | Q9EQW6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 108 – 162 | bHLHPROSITE-ProRule annotationAdd BLAST | 55 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 77 – 88 | Poly-SerAdd BLAST | 12 | |
Compositional biasi | 206 – 212 | Poly-Ala | 7 | |
Compositional biasi | 225 – 237 | Poly-AlaAdd BLAST | 13 | |
Compositional biasi | 266 – 270 | Poly-Ala | 5 | |
Compositional biasi | 273 – 284 | Poly-GlyAdd BLAST | 12 |
Domaini
The bHLH is essential for interaction with NKX2-2.
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161651 |
HOGENOMi | CLU_065376_1_0_1 |
InParanoidi | Q9EQW6 |
OMAi | PVHHPIL |
OrthoDBi | 1290623at2759 |
PhylomeDBi | Q9EQW6 |
TreeFami | TF322733 |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032658, Olig2 |
PANTHERi | PTHR19290:SF32, PTHR19290:SF32, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
i Sequence
Sequence statusi: Complete.
Q9EQW6-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDSDASLVSS RPSSPEPDDL FLPARSKGGS SSGFTGGTVS SSTPSDCPPE
60 70 80 90 100
LSSELRGAMG ASGAHPGDKL GGGGFKSSSS STSSSTSSAA TSSTKKDKKQ
110 120 130 140 150
MTEPELQQLR LKINSRERKR MHDLNIAMDG LREVMPYAHG PSVRKLSKIA
160 170 180 190 200
TLLLARNYIL MLTNSLEEMK RLVSEIYGGH HAGFHPSACG GLAHSAPLPT
210 220 230 240 250
ATAHPAAAAH AAHHPAVHHP ILPPAAAAAA AAAAAAAVSS ASLPGSGLSS
260 270 280 290 300
VGSIRPPHGL LKSPSAAAAA PLGGGGGGSG GSGGFQHWGG MPCPCSMCQV
310 320
PPPHHHVSAM GAGTLPRLTS DAK
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 198 | L → I in AAF61722 (PubMed:10719889).Curated | 1 | |
Sequence conflicti | 205 | P → PTRHGAP (PubMed:10719889).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038697 mRNA Translation: BAB18907.1 AF232929 Genomic DNA Translation: AAF61722.1 BC051967 mRNA Translation: AAH51967.1 |
CCDSi | CCDS28323.1 |
RefSeqi | NP_058663.2, NM_016967.2 |
Genome annotation databases
Ensembli | ENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830 |
GeneIDi | 50913 |
KEGGi | mmu:50913 |
UCSCi | uc007zxe.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB038697 mRNA Translation: BAB18907.1 AF232929 Genomic DNA Translation: AAF61722.1 BC051967 mRNA Translation: AAH51967.1 |
CCDSi | CCDS28323.1 |
RefSeqi | NP_058663.2, NM_016967.2 |
3D structure databases
SMRi | Q9EQW6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 206152, 8 interactors |
IntActi | Q9EQW6, 4 interactors |
STRINGi | 10090.ENSMUSP00000036797 |
PTM databases
iPTMneti | Q9EQW6 |
PhosphoSitePlusi | Q9EQW6 |
Proteomic databases
PaxDbi | Q9EQW6 |
PRIDEi | Q9EQW6 |
Protocols and materials databases
Antibodypediai | 917, 468 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000035608; ENSMUSP00000036797; ENSMUSG00000039830 |
GeneIDi | 50913 |
KEGGi | mmu:50913 |
UCSCi | uc007zxe.1, mouse |
Organism-specific databases
CTDi | 10215 |
MGIi | MGI:1355331, Olig2 |
Phylogenomic databases
eggNOGi | KOG3898, Eukaryota |
GeneTreei | ENSGT00940000161651 |
HOGENOMi | CLU_065376_1_0_1 |
InParanoidi | Q9EQW6 |
OMAi | PVHHPIL |
OrthoDBi | 1290623at2759 |
PhylomeDBi | Q9EQW6 |
TreeFami | TF322733 |
Miscellaneous databases
BioGRID-ORCSi | 50913, 1 hit in 17 CRISPR screens |
ChiTaRSi | Olig2, mouse |
PROi | PR:Q9EQW6 |
RNActi | Q9EQW6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000039830, Expressed in lumbar subsegment of spinal cord and 123 other tissues |
ExpressionAtlasi | Q9EQW6, baseline and differential |
Genevisiblei | Q9EQW6, MM |
Family and domain databases
CDDi | cd00083, HLH, 1 hit |
Gene3Di | 4.10.280.10, 1 hit |
InterProi | View protein in InterPro IPR011598, bHLH_dom IPR036638, HLH_DNA-bd_sf IPR032658, Olig2 |
PANTHERi | PTHR19290:SF32, PTHR19290:SF32, 1 hit |
Pfami | View protein in Pfam PF00010, HLH, 1 hit |
SMARTi | View protein in SMART SM00353, HLH, 1 hit |
SUPFAMi | SSF47459, SSF47459, 1 hit |
PROSITEi | View protein in PROSITE PS50888, BHLH, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | OLIG2_MOUSE | |
Accessioni | Q9EQW6Primary (citable) accession number: Q9EQW6 Secondary accession number(s): Q9JKN4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 13, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 151 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot