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Protein

Tripeptidyl-peptidase 1

Gene

Tpp1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Lysosomal serine protease with tripeptidyl-peptidase I activity. May act as a non-specific lysosomal peptidase which generates tripeptides from the breakdown products produced by lysosomal proteinases. Requires substrates with an unsubstituted N-terminus.1 Publication

Catalytic activityi

Release of an N-terminal tripeptide from a polypeptide, but also has endopeptidase activity.

Cofactori

Ca2+By similarityNote: Binds 1 Ca2+ ion per subunit.By similarity

pH dependencei

Optimum pH is 4. Unstable above pH 7.1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei272Charge relay systemBy similarity1
Active sitei276Charge relay systemBy similarity1
Active sitei475Charge relay systemBy similarity1
Metal bindingi517CalciumBy similarity1
Metal bindingi518Calcium; via carbonyl oxygenBy similarity1
Metal bindingi539Calcium; via carbonyl oxygenBy similarity1
Metal bindingi541Calcium; via carbonyl oxygenBy similarity1
Metal bindingi543CalciumBy similarity1

GO - Molecular functioni

  • endopeptidase activity Source: RGD
  • metal ion binding Source: UniProtKB-KW
  • peptidase activity Source: UniProtKB
  • peptide binding Source: RGD
  • serine-type endopeptidase activity Source: InterPro
  • serine-type peptidase activity Source: UniProtKB
  • tripeptidyl-peptidase activity Source: RGD

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Protease, Serine protease
LigandCalcium, Metal-binding

Protein family/group databases

MEROPSiS53.003

Names & Taxonomyi

Protein namesi
Recommended name:
Tripeptidyl-peptidase 1 (EC:3.4.14.9)
Short name:
TPP-1
Alternative name(s):
Tripeptidyl aminopeptidase
Tripeptidyl-peptidase I
Short name:
TPP-I
Gene namesi
Name:Tpp1
Synonyms:Cln2
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi621296 Tpp1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Lysosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
PropeptideiPRO_000002738420 – 195Removed in mature form1 PublicationAdd BLAST176
ChainiPRO_0000027385196 – 563Tripeptidyl-peptidase 1Add BLAST368

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi111 ↔ 122By similarity
Glycosylationi210N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi222N-linked (GlcNAc...) asparagineCombined sources1
Glycosylationi286N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi313N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi365 ↔ 526By similarity
Glycosylationi443N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi522 ↔ 537By similarity

Post-translational modificationi

Activated by autocatalytic proteolytical processing upon acidification. N-glycosylation is required for processing and activity (By similarity).By similarity

Keywords - PTMi

Autocatalytic cleavage, Disulfide bond, Glycoprotein, Zymogen

Proteomic databases

PaxDbiQ9EQV6
PRIDEiQ9EQV6

PTM databases

iPTMnetiQ9EQV6
PhosphoSitePlusiQ9EQV6
UniCarbKBiQ9EQV6

Interactioni

Subunit structurei

Monomer. Interacts with CLN5.By similarity

Protein-protein interaction databases

IntActiQ9EQV6, 1 interactor
STRINGi10116.ENSRNOP00000026280

Structurei

3D structure databases

ProteinModelPortaliQ9EQV6
SMRiQ9EQV6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini199 – 563Peptidase S53Add BLAST365

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IICY Eukaryota
COG4934 LUCA
HOGENOMiHOG000171253
HOVERGENiHBG004449
InParanoidiQ9EQV6
KOiK01279
PhylomeDBiQ9EQV6

Family and domain databases

CDDicd04056 Peptidases_S53, 1 hit
cd11377 Pro-peptidase_S53, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR036852 Peptidase_S8/S53_dom_sf
IPR015366 S53_propep
IPR030400 Sedolisin_dom
PfamiView protein in Pfam
PF09286 Pro-kuma_activ, 1 hit
SMARTiView protein in SMART
SM00944 Pro-kuma_activ, 1 hit
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51695 SEDOLISIN, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9EQV6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MGLQARFLGL LALVIAGKCT HSPEPDQRWM LPPGWVSLGR VDPEEELSLT
60 70 80 90 100
FALKQQNLDR LSELVQAVSD PSSPRYGKYL TLEDVAELVQ PSPLTLRTVQ
110 120 130 140 150
KWLLAAGARD CHSVTTQDFL TCWLSVRQAE LLLPGAEFHR YVGGPAKTHI
160 170 180 190 200
IRSPHPYQLP QALAPHVDLV AGLHRFPPLS SPRQRPEPQG VGPVGLHLGV
210 220 230 240 250
TPSVLRQRYN LTARDVGSGT TNNSQACAQF LEQYFHNSDL TEFMRLFGSS
260 270 280 290 300
FAHQASVARV VGKQGRGRAG IEASLDVEYL MSAGANISTW VYSSPGRHEA
310 320 330 340 350
QEPFLQWLLL LSNESSLPHV HTVSYGDDED SLSSVYIQRV NTEFMKAAAR
360 370 380 390 400
GLTLLFASGD TGAGCWSVSG RHKFRPSFPA SSPYVTTVGG TSFKNPFLVT
410 420 430 440 450
NEVVDYISGG GFSNVFPQPS YQEEAVAQFL KSSSHLPPSS YFNASGRAYP
460 470 480 490 500
DVAALSDGYW VVSNMVPIPW VSGTSASTPV FGGILSLINE HRLLNGRPPL
510 520 530 540 550
GFLNPRLYQQ HGAGLFDVTH GCHESCLNEE VEGQGFCSGP GWDPVTGWGT
560
PNFPALLKTL LNP
Length:563
Mass (Da):61,332
Last modified:March 1, 2001 - v1
Checksum:iB54F3C86205DFEC1
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
Q642E6Q642E6_RAT
Tripeptidyl peptidase I
Tpp1 rCG_39632
563Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti210N → A AA sequence (PubMed:9659384).Curated1
Sequence conflicti216 – 217VG → SQ AA sequence (PubMed:9659384).Curated2
Sequence conflicti389 – 391GGT → SPP AA sequence (PubMed:9659384).Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043870 mRNA Translation: BAB18570.1
RefSeqiNP_112647.1, NM_031357.1
UniGeneiRn.162472

Genome annotation databases

GeneIDi83534
KEGGirno:83534
UCSCiRGD:621296 rat

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB043870 mRNA Translation: BAB18570.1
RefSeqiNP_112647.1, NM_031357.1
UniGeneiRn.162472

3D structure databases

ProteinModelPortaliQ9EQV6
SMRiQ9EQV6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiQ9EQV6, 1 interactor
STRINGi10116.ENSRNOP00000026280

Protein family/group databases

MEROPSiS53.003

PTM databases

iPTMnetiQ9EQV6
PhosphoSitePlusiQ9EQV6
UniCarbKBiQ9EQV6

Proteomic databases

PaxDbiQ9EQV6
PRIDEiQ9EQV6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi83534
KEGGirno:83534
UCSCiRGD:621296 rat

Organism-specific databases

CTDi1200
RGDi621296 Tpp1

Phylogenomic databases

eggNOGiENOG410IICY Eukaryota
COG4934 LUCA
HOGENOMiHOG000171253
HOVERGENiHBG004449
InParanoidiQ9EQV6
KOiK01279
PhylomeDBiQ9EQV6

Miscellaneous databases

PROiPR:Q9EQV6

Family and domain databases

CDDicd04056 Peptidases_S53, 1 hit
cd11377 Pro-peptidase_S53, 1 hit
Gene3Di3.40.50.200, 1 hit
InterProiView protein in InterPro
IPR036852 Peptidase_S8/S53_dom_sf
IPR015366 S53_propep
IPR030400 Sedolisin_dom
PfamiView protein in Pfam
PF09286 Pro-kuma_activ, 1 hit
SMARTiView protein in SMART
SM00944 Pro-kuma_activ, 1 hit
SUPFAMiSSF52743 SSF52743, 1 hit
PROSITEiView protein in PROSITE
PS51695 SEDOLISIN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTPP1_RAT
AccessioniPrimary (citable) accession number: Q9EQV6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 31, 2002
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 107 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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