UniProtKB - Q9EQN5 (SMBP2_RAT)
DNA-binding protein SMUBP-2
Ighmbp2
Functioni
5' to 3' helicase that unwinds RNA and DNA duplexes in an ATP-dependent reaction (By similarity).
Specific to 5'-phosphorylated single-stranded guanine-rich sequences (By similarity).
May play a role in RNA metabolism, ribosome biogenesis or initiation of translation (By similarity).
May play a role in regulation of transcription (PubMed:11106437).
Interacts with tRNA-Tyr (By similarity).
By similarity1 PublicationCatalytic activityi
- EC:3.6.4.12By similarity EC:3.6.4.13By similarityThis reaction proceeds in the forwardBy similarity direction.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 402 | ATPBy similarity | 1 | |
Binding sitei | 441 | ATPBy similarity | 1 | |
Binding sitei | 570 | ATPBy similarity | 1 | |
Metal bindingi | 890 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 895 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 906 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 909 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 914 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 917 | Zinc 1PROSITE-ProRule annotation | 1 | |
Metal bindingi | 923 | Zinc 2PROSITE-ProRule annotation | 1 | |
Metal bindingi | 925 | Zinc 2PROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 213 – 220 | ATPPROSITE-ProRule annotation | 8 | |
Zinc fingeri | 884 – 933 | AN1-typePROSITE-ProRule annotationAdd BLAST | 50 |
GO - Molecular functioni
- 5'-3' DNA helicase activity Source: UniProtKB
- 5'-3' RNA helicase activity Source: UniProtKB
- ATP binding Source: UniProtKB
- ATP-dependent activity, acting on DNA Source: UniProtKB
- ATP-dependent activity, acting on RNA Source: UniProtKB
- ATP hydrolysis activity Source: UniProtKB
- DNA binding Source: RGD
- double-stranded DNA helicase activity Source: UniProtKB
- G-rich single-stranded DNA binding Source: RGD
- identical protein binding Source: RGD
- protein self-association Source: UniProtKB
- ribosome binding Source: UniProtKB
- RNA binding Source: UniProtKB
- single-stranded DNA binding Source: UniProtKB
- single-stranded RNA binding Source: UniProtKB
- tRNA binding Source: UniProtKB
- zinc ion binding Source: InterPro
GO - Biological processi
- DNA duplex unwinding Source: UniProtKB
- negative regulation of transcription by RNA polymerase II Source: RGD
- neuromuscular process Source: RGD
- spinal cord motor neuron differentiation Source: RGD
Keywordsi
Molecular function | Activator, DNA-binding, Helicase, Hydrolase, Ribonucleoprotein, RNA-binding, tRNA-binding |
Biological process | Transcription, Transcription regulation |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Ighmbp2 Synonyms:Smbp2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 68325, Ighmbp2 |
Subcellular locationi
Nucleus
- nucleus Source: UniProtKB
Other locations
- axon Source: RGD
- cytoplasm Source: UniProtKB
- growth cone Source: RGD
- neuronal cell body Source: RGD
- perinuclear region of cytoplasm Source: RGD
- ribonucleoprotein complex Source: RGD
Keywords - Cellular componenti
Cell projection, Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000372431 | 2 – 988 | DNA-binding protein SMUBP-2Add BLAST | 987 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 797 | PhosphoserineBy similarity | 1 | |
Modified residuei | 800 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9EQN5 |
PRIDEi | Q9EQN5 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Homooligomer (By similarity).
Interacts with RUVBL1 (By similarity).
Interacts with RUVBL2 (By similarity).
Interacts with GTF3C1 (By similarity).
Interacts with ABT1 (By similarity).
Interacts with ribosomes (By similarity).
By similarityGO - Molecular functioni
- identical protein binding Source: RGD
- protein self-association Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018487 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 721 – 784 | R3HPROSITE-ProRule annotationAdd BLAST | 64 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 637 – 783 | SS DNA-bindingBy similarityAdd BLAST | 147 | |
Regioni | 651 – 722 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 765 – 820 | DisorderedSequence analysisAdd BLAST | 56 | |
Regioni | 835 – 872 | DisorderedSequence analysisAdd BLAST | 38 | |
Regioni | 943 – 988 | DisorderedSequence analysisAdd BLAST | 46 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 957 – 986 | Sequence analysisAdd BLAST | 30 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 857 – 861 | Nuclear localization signalSequence analysis | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 698 – 717 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 765 – 779 | Basic and acidic residuesSequence analysisAdd BLAST | 15 | |
Compositional biasi | 791 – 806 | Pro residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 835 – 850 | Polar residuesSequence analysisAdd BLAST | 16 | |
Compositional biasi | 952 – 988 | Basic and acidic residuesSequence analysisAdd BLAST | 37 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 884 – 933 | AN1-typePROSITE-ProRule annotationAdd BLAST | 50 |
Keywords - Domaini
Coiled coil, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1803, Eukaryota |
InParanoidi | Q9EQN5 |
OrthoDBi | 633768at2759 |
PhylomeDBi | Q9EQN5 |
Family and domain databases
CDDi | cd02641, R3H_Smubp-2_like, 1 hit cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit 3.40.50.300, 2 hits 4.10.1110.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR035896, AN1-like_Znf IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR014001, Helicase_ATP-bd IPR027417, P-loop_NTPase IPR001374, R3H_dom IPR036867, R3H_dom_sf IPR034072, R3H_Smubp-2 IPR004483, SMUBP-2/Hcs1-like IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF13086, AAA_11, 1 hit PF13087, AAA_12, 1 hit PF01424, R3H, 1 hit PF01428, zf-AN1, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00487, DEXDc, 1 hit SM00393, R3H, 1 hit SM00154, ZnF_AN1, 1 hit |
SUPFAMi | SSF118310, SSF118310, 1 hit SSF52540, SSF52540, 1 hit SSF82708, SSF82708, 1 hit |
TIGRFAMsi | TIGR00376, TIGR00376, 1 hit |
PROSITEi | View protein in PROSITE PS51061, R3H, 1 hit PS51039, ZF_AN1, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 3 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MASYTVESFV AQQLQLLELE RDAEVEERRS WQEHSSLKEL QSRGVCLLKL
60 70 80 90 100
QVSGQRTGLY GQRLVTFEPR KFGPAVVLPS NSFTSGDIVG LYDTNESSQL
110 120 130 140 150
ATGVLTRITQ KSVIVAFDES HDFQLNLDRE NTYRLLKLAN DVTYKRLKKA
160 170 180 190 200
LLTLKKYHSG PASSLIDVLL GGSTPSPATE IPPLTFYNTT LDPSQKEAVS
210 220 230 240 250
FALAQKEVAI IHGPPGTGKT TTVVEIILQA VKQGLKVLCC APSNIAVDNL
260 270 280 290 300
VERLALCKKQ ILRLGHPARL LESVQQHSLD AVLARSDNAQ IVADIRRDID
310 320 330 340 350
QVFGKNKKTQ DKREKSNFRN EIKLLRKELK EREEAAIVQS LSAADVVLAT
360 370 380 390 400
NTGASTDGPL KLLPEDYFDV VVVDECAQAL EASCWIPLLK APKCILAGDH
410 420 430 440 450
KQLPPTTVSH KAALAGLSRS LMERLAEKHG AAVVRMLAVQ YRMHQAITRW
460 470 480 490 500
ASEAMYHGQL TAHPSVAGHL LKDLPGVADT EETSVPLLLI DTAGCGLLEL
510 520 530 540 550
EEEDSQSKGN PGEVRLVTLH IQALVDAGVQ AGDIAVIAPY NLQVDLLRQS
560 570 580 590 600
LSNKHPELEI KSVDGFQGRE KEAVILTFVR SNRKGEVGFL AEDRRINVAV
610 620 630 640 650
TRARRHVAVI CDSHTVNNHA FLKTLVDYFT EHGEVRTAFE YLDDIVPENY
660 670 680 690 700
THEGSRSHSC APKPKCPTTS VRKPASAQES RQEARAATGH SRRKPSEKPL
710 720 730 740 750
GSQVQPQHSS KANGSDRTGG TDRTEHFRAM IEEFVASKEA QLEFPTSLSS
760 770 780 790 800
HDRLRVHQLA EEFGLKHDST GEGKARHITV SRRSPAGSGS ATPQPPSPPS
810 820 830 840 850
PAQAEPEPQV EQPVGQPHGS TQLDLKALHL ERLQRQQGCQ AQSQLGGGSR
860 870 880 890 900
PQKAPQKKKK KEPKGPAMAL PSEEDFDALV SAVVKADNTC SFTKCSASTT
910 920 930 940 950
TLGQFCMHCS RRYCLSHHLP EIHGCGEKAR AHARQRISRE GVLYAGSGTK
960 970 980
DRALDPAKRA QLQRKLDKKL GELSSQRTSK KKEKERGT
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1LQE6 | F1LQE6_RAT | DNA-binding protein SMUBP-2 | Ighmbp2 | 932 | Annotation score: | ||
A0A0G2JVR3 | A0A0G2JVR3_RAT | DNA-binding protein SMUBP-2 | Ighmbp2 | 869 | Annotation score: | ||
A0A8I6B3F7 | A0A8I6B3F7_RAT | DNA-binding protein SMUBP-2 | Ighmbp2 | 907 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF199411 mRNA Translation: AAG28561.1 |
RefSeqi | NP_113774.1, NM_031586.1 |
Genome annotation databases
GeneIDi | 29532 |
KEGGi | rno:29532 |
UCSCi | RGD:68325, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF199411 mRNA Translation: AAG28561.1 |
RefSeqi | NP_113774.1, NM_031586.1 |
3D structure databases
AlphaFoldDBi | Q9EQN5 |
SMRi | Q9EQN5 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000018487 |
Proteomic databases
PaxDbi | Q9EQN5 |
PRIDEi | Q9EQN5 |
Genome annotation databases
GeneIDi | 29532 |
KEGGi | rno:29532 |
UCSCi | RGD:68325, rat |
Organism-specific databases
CTDi | 3508 |
RGDi | 68325, Ighmbp2 |
Phylogenomic databases
eggNOGi | KOG1803, Eukaryota |
InParanoidi | Q9EQN5 |
OrthoDBi | 633768at2759 |
PhylomeDBi | Q9EQN5 |
Miscellaneous databases
PROi | PR:Q9EQN5 |
Family and domain databases
CDDi | cd02641, R3H_Smubp-2_like, 1 hit cd18808, SF1_C_Upf1, 1 hit |
Gene3Di | 3.30.1370.50, 1 hit 3.40.50.300, 2 hits 4.10.1110.10, 1 hit |
InterProi | View protein in InterPro IPR003593, AAA+_ATPase IPR035896, AN1-like_Znf IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR014001, Helicase_ATP-bd IPR027417, P-loop_NTPase IPR001374, R3H_dom IPR036867, R3H_dom_sf IPR034072, R3H_Smubp-2 IPR004483, SMUBP-2/Hcs1-like IPR000058, Znf_AN1 |
Pfami | View protein in Pfam PF13086, AAA_11, 1 hit PF13087, AAA_12, 1 hit PF01424, R3H, 1 hit PF01428, zf-AN1, 1 hit |
SMARTi | View protein in SMART SM00382, AAA, 1 hit SM00487, DEXDc, 1 hit SM00393, R3H, 1 hit SM00154, ZnF_AN1, 1 hit |
SUPFAMi | SSF118310, SSF118310, 1 hit SSF52540, SSF52540, 1 hit SSF82708, SSF82708, 1 hit |
TIGRFAMsi | TIGR00376, TIGR00376, 1 hit |
PROSITEi | View protein in PROSITE PS51061, R3H, 1 hit PS51039, ZF_AN1, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SMBP2_RAT | |
Accessioni | Q9EQN5Primary (citable) accession number: Q9EQN5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 5, 2009 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families