Dgcr8

Functionⁱ

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).By similarity1 Publication

Cofactorⁱ

hemeBy similarityNote: Binds 1 heme group per homodimer.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Metal bindingⁱ	352	Iron (heme axial ligand)By similarity		1

GO - Molecular functionⁱ

double-stranded RNA binding Source: MGI
heme binding Source: MGI
identical protein binding Source: MGI
metal ion binding Source: UniProtKB-KW
primary miRNA binding Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

primary miRNA processing Source: MGI
regulation of stem cell proliferation
Source: MGI
Inferred from mutant phenotypeⁱ
- 21609717
RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	RNA-binding
Ligand	Heme, Iron, Metal-binding

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Microprocessor complex subunit DGCR8 Alternative name(s): DiGeorge syndrome critical region 8 homolog Gy1
Gene namesⁱ	Name:Dgcr8
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 16

Organism-specific databases

MGIⁱ	MGI:2151114 Dgcr8

Keywords - Cellular componentⁱ

Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000079879	1 – 773	Microprocessor complex subunit DGCR8Add BLAST		773

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	35	PhosphoserineBy similarity	1
Modified residueⁱ	92	PhosphoserineBy similarity	1
Modified residueⁱ	95	PhosphoserineCombined sources	1
Modified residueⁱ	271	PhosphoserineCombined sources	1
Modified residueⁱ	275	PhosphoserineCombined sources	1
Modified residueⁱ	279	PhosphothreonineCombined sources	1
Modified residueⁱ	373	PhosphoserineBy similarity	1
Modified residueⁱ	377	PhosphoserineCombined sources	1
Cross-linkⁱ	424	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	500	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linkⁱ	707	Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMⁱ

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDⁱ	Q9EQM6
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9EQM6
PaxDbⁱ	Q9EQM6
PeptideAtlas More...PeptideAtlasⁱ	Q9EQM6
PRIDEⁱ	Q9EQM6

PTM databases

iPTMnetⁱ	Q9EQM6
PhosphoSitePlusⁱ	Q9EQM6

Expressionⁱ

Tissue specificityⁱ

Ubiquitously expressed.1 Publication

Developmental stageⁱ

Expressed in embryonic stem cells. During embryo development it is expressed in neuroepithelium of primary brain, limb bud, vessels, thymus, and around the palate.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000022718 Expressed in 244 organ(s), highest expression level in ear vesicle
Genevisibleⁱ	Q9EQM6 MM

Interactionⁱ

Subunit structureⁱ

Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization. Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with ILF3, NCL and DROSHA.By similarity

GO - Molecular functionⁱ

identical protein binding Source: MGI
protein homodimerization activity Source: MGI

View the complete GO annotation on QuickGO ...

Protein-protein interaction databases

BioGridⁱ	220480, 2 interactors
ComplexPortalⁱ	CPX-3081 Microprocessor complex
CORUMⁱ	Q9EQM6
IntActⁱ	Q9EQM6, 1 interactor
STRINGⁱ	10090.ENSMUSP00000009321

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9EQM6
SMRⁱ	Q9EQM6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Length
Domainⁱ	301 – 334	WWPROSITE-ProRule annotationAdd BLAST	34
Domainⁱ	511 – 578	DRBM 1PROSITE-ProRule annotationAdd BLAST	68
Domainⁱ	620 – 685	DRBM 2PROSITE-ProRule annotationAdd BLAST	66

Region

Feature key	Position(s)	DescriptionActions	Length
Regionⁱ	1 – 342	Necessary for interaction with NCLBy similarityAdd BLAST	342
Regionⁱ	1 – 275	Necessary for nuclear localization and retentionBy similarityAdd BLAST	275
Regionⁱ	276 – 751	Necessary for heme-binding and pri-miRNA processingBy similarityAdd BLAST	476
Regionⁱ	701 – 773	Interaction with DROSHABy similarityAdd BLAST	73

Keywords - Domainⁱ

Repeat

Phylogenomic databases

eggNOGⁱ	KOG4334 Eukaryota ENOG410XS9I LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00390000015977
HOGENOMⁱ	HOG000232104
HOVERGENⁱ	HBG051344
InParanoidⁱ	Q9EQM6
KEGG Orthology (KO) More...KOⁱ	K18419
OMAⁱ	ETRVVTW
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G09GA
PhylomeDBⁱ	Q9EQM6
TreeFamⁱ	TF324256

Family and domain databases

Conserved Domains Database More...CDDⁱ	cd00048 DSRM, 1 hit cd00201 WW, 1 hit
InterProⁱ	View protein in InterPro IPR014720 dsRBD_dom IPR001202 WW_dom IPR036020 WW_dom_sf
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00035 dsrm, 2 hits
SMARTⁱ	View protein in SMART SM00358 DSRM, 2 hits SM00456 WW, 1 hit
SUPFAMⁱ	SSF51045 SSF51045, 1 hit
PROSITEⁱ	View protein in PROSITE PS50137 DS_RBD, 1 hit PS50020 WW_DOMAIN_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q9EQM6-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
METYESPSPL PREPAGEAMM ENRACPFQVL PHEQSPPPPL QTSSDAEVMD 
        60         70         80         90        100
VGSGGDGQSE PPADDPFNFY GASLLSKGSF SKGRLLIDPN CSGHSPRTAR 
       110        120        130        140        150
HAPAVRKFSP DLKLLKDVKI SVSFTESCRS KDRKVLYTGV ERSTRPECGQ 
       160        170        180        190        200
LLSPVSGDVH ACPFGGSVGN GVGLGGESAD KKDEENELDQ EKRVEYAVLD 
       210        220        230        240        250
ELEDFTDNLE LDEEGTGGFT AKAIVQRDRV DEEALNFSYE DDFDNDVDAL 
       260        270        280        290        300
LEEGLCAPKK RRMEEKYGGD SDHPSDGETS VQPMMTKIKT VLKSRGRPPT 
       310        320        330        340        350
EPLPDGWIMT FHNSGVPVYL HRESRVVTWS RPYFLGTGSI RKHDPPLSSI 
       360        370        380        390        400
PCLHYKKMKD NEEREQNCDL APSGEVSPVK PLGRSAELDF PLEEPDSMGG 
       410        420        430        440        450
DSGSMDEKDP LGAEAAAGAL GQVKAKVEVC KDESVDLEEF RNYLEKRFDF 
       460        470        480        490        500
EQVTVKKFRT WAERRQFNRE MKRKQAESER PILPANQKLI TLSVQDAPTK 
       510        520        530        540        550
KEFVINPNGK SEVCILHEYM QRVLKVRPVY NFFECENPSE PFGASVTIDG 
       560        570        580        590        600
VTYGSGTASS KKLAKNKAAR ATLEILIPDF VKQTSEEKPK DSEELEYFNH 
       610        620        630        640        650
ISIEDSRVYE LTSKAGLLSP YQILHECLKR NHGMGDTSIK FEVVPGKNQK 
       660        670        680        690        700
SEYVMACGKH TVRGWCKNKR VGKQLASQKI LQLLHPHVKN WGSLLRMYGR 
       710        720        730        740        750
ESSKMVKQET SDKSVIELQQ YAKKNRPNLH ILSKLQEEMK RLAAEREETR 
       760        770 
KKPKMSIVAS AQPGGEPLCT VDV

Length:773

Mass (Da):86,321

Last modified:July 19, 2004 - v2

Checksum:ⁱDA837BD6017B0446

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	A0A338P666	A0A338P666_MOUSE		Microprocessor complex subunit DGCR... Microprocessor complex subunit DGCR8	Dgcr8	141	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	241 – 243	DDF → VCW in AAG35599 (Ref. 3) Curated		3

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB086857 mRNA Translation: BAD06468.1 BC062919 mRNA Translation: AAH62919.1 BC066118 mRNA Translation: AAH66118.1 AF201683 mRNA Translation: AAG35599.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS28018.1
NCBI Reference Sequences More...RefSeqⁱ	NP_201581.2, NM_033324.2
UniGeneⁱ	Mm.447352 Mm.482156

Genome annotation databases

Ensemblⁱ	ENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718 ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687
GeneIDⁱ	94223
KEGGⁱ	mmu:94223
UCSC genome browser More...UCSCⁱ	uc007yne.1 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9EQM6	Microprocessor complex subunit DGCR8	)	HUMAN		773	UniRef90_Q8WYQ5
Microprocessor complex subunit DGCR8		BOVIN		760
DGCR8 isoform 2		PANTR		773
Uncharacterized protein		PANPA		773
Microprocessor complex subunit DGCR8		MYODS		799
+150

Protein	Similar proteins		Species	Score	Length	Source
Q9EQM6	Microprocessor complex subunit DGCR8	)	HUMAN		773	UniRef50_Q8WYQ5
Microprocessor complex subunit DGCR8		BOVIN		760
DGCR8 isoform 2		PANTR		773
Uncharacterized protein		PANPA		773
Microprocessor complex subunit DGCR8		MYODS		799
+400

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AB086857 mRNA Translation: BAD06468.1 BC062919 mRNA Translation: AAH62919.1 BC066118 mRNA Translation: AAH66118.1 AF201683 mRNA Translation: AAG35599.1
CCDSⁱ	CCDS28018.1
RefSeqⁱ	NP_201581.2, NM_033324.2
UniGeneⁱ	Mm.447352 Mm.482156

3D structure databases

ProteinModelPortalⁱ	Q9EQM6
SMRⁱ	Q9EQM6
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	220480, 2 interactors
ComplexPortalⁱ	CPX-3081 Microprocessor complex
CORUMⁱ	Q9EQM6
IntActⁱ	Q9EQM6, 1 interactor
STRINGⁱ	10090.ENSMUSP00000009321

PTM databases

iPTMnetⁱ	Q9EQM6
PhosphoSitePlusⁱ	Q9EQM6

Proteomic databases

EPDⁱ	Q9EQM6
MaxQBⁱ	Q9EQM6
PaxDbⁱ	Q9EQM6
PeptideAtlasⁱ	Q9EQM6
PRIDEⁱ	Q9EQM6

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718 ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687
GeneIDⁱ	94223
KEGGⁱ	mmu:94223
UCSCⁱ	uc007yne.1 mouse

Organism-specific databases

CTDⁱ	54487
MGIⁱ	MGI:2151114 Dgcr8

Phylogenomic databases

eggNOGⁱ	KOG4334 Eukaryota ENOG410XS9I LUCA
GeneTreeⁱ	ENSGT00390000015977
HOGENOMⁱ	HOG000232104
HOVERGENⁱ	HBG051344
InParanoidⁱ	Q9EQM6
KOⁱ	K18419
OMAⁱ	ETRVVTW
OrthoDBⁱ	EOG091G09GA
PhylomeDBⁱ	Q9EQM6
TreeFamⁱ	TF324256

Miscellaneous databases

ChiTaRSⁱ	Dgcr8 mouse
Protein Ontology More...PROⁱ	PR:Q9EQM6
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000022718 Expressed in 244 organ(s), highest expression level in ear vesicle
Genevisibleⁱ	Q9EQM6 MM

Family and domain databases

CDDⁱ	cd00048 DSRM, 1 hit cd00201 WW, 1 hit
InterProⁱ	View protein in InterPro IPR014720 dsRBD_dom IPR001202 WW_dom IPR036020 WW_dom_sf
Pfamⁱ	View protein in Pfam PF00035 dsrm, 2 hits
SMARTⁱ	View protein in SMART SM00358 DSRM, 2 hits SM00456 WW, 1 hit
SUPFAMⁱ	SSF51045 SSF51045, 1 hit
PROSITEⁱ	View protein in PROSITE PS50137 DS_RBD, 1 hit PS50020 WW_DOMAIN_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	DGCR8_MOUSE
Accessionⁱ	Q9EQM6Primary (citable) accession number: Q9EQM6
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 10, 2002
	Last sequence update:	July 19, 2004
	Last modified:	November 7, 2018
	This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9EQM6 (DGCR8_MOUSE)

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Microprocessor complex subunit DGCR8

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

GO - Molecular functioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Region

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

GO - Molecular functionⁱ

Structureⁱ

Family & Domainsⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ