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Protein

Microprocessor complex subunit DGCR8

Gene

Dgcr8

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the microprocessor complex that acts as a RNA- and heme-binding protein that is involved in the initial step of microRNA (miRNA) biogenesis (PubMed:17259983). Component of the microprocessor complex that is required to process primary miRNA transcripts (pri-miRNAs) to release precursor miRNA (pre-miRNA) in the nucleus. Within the microprocessor complex, DGCR8 function as a molecular anchor necessary for the recognition of pri-miRNA at dsRNA-ssRNA junction and directs DROSHA to cleave 11 bp away form the junction to release hairpin-shaped pre-miRNAs that are subsequently cut by the cytoplasmic DICER to generate mature miRNAs. The heme-bound DGCR8 dimer binds pri-miRNAs as a cooperative trimer (of dimers) and is active in triggering pri-miRNA cleavage, whereas the heme-free DGCR8 monomer binds pri-miRNAs as a dimer and is much less active. Both double-stranded and single-stranded regions of a pri-miRNA are required for its binding. Specifically recognizes and binds N6-methyladenosine (m6A)-containing pri-miRNAs, a modification required for pri-miRNAs processing (By similarity). Involved in the silencing of embryonic stem cell self-renewal (PubMed:17259983).By similarity1 Publication

Cofactori

hemeBy similarityNote: Binds 1 heme group per homodimer.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi352Iron (heme axial ligand)By similarity1

GO - Molecular functioni

GO - Biological processi

  • primary miRNA processing Source: MGI
  • regulation of stem cell proliferation Source: MGI
  • RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC

Keywordsi

Molecular functionRNA-binding
LigandHeme, Iron, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Microprocessor complex subunit DGCR8
Alternative name(s):
DiGeorge syndrome critical region 8 homolog
Gy1
Gene namesi
Name:Dgcr8
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 16

Organism-specific databases

MGIiMGI:2151114 Dgcr8

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000798791 – 773Microprocessor complex subunit DGCR8Add BLAST773

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei35PhosphoserineBy similarity1
Modified residuei92PhosphoserineBy similarity1
Modified residuei95PhosphoserineCombined sources1
Modified residuei271PhosphoserineCombined sources1
Modified residuei275PhosphoserineCombined sources1
Modified residuei279PhosphothreonineCombined sources1
Modified residuei373PhosphoserineBy similarity1
Modified residuei377PhosphoserineCombined sources1
Cross-linki424Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki500Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity
Cross-linki707Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity

Keywords - PTMi

Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiQ9EQM6
MaxQBiQ9EQM6
PaxDbiQ9EQM6
PeptideAtlasiQ9EQM6
PRIDEiQ9EQM6

PTM databases

iPTMnetiQ9EQM6
PhosphoSitePlusiQ9EQM6

Expressioni

Tissue specificityi

Ubiquitously expressed.1 Publication

Developmental stagei

Expressed in embryonic stem cells. During embryo development it is expressed in neuroepithelium of primary brain, limb bud, vessels, thymus, and around the palate.2 Publications

Gene expression databases

BgeeiENSMUSG00000022718 Expressed in 244 organ(s), highest expression level in ear vesicle
GenevisibleiQ9EQM6 MM

Interactioni

Subunit structurei

Monomer; in absence of heme. Homodimer; the association with heme promotes its dimerization. Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8. The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region). Interacts with ILF3, NCL and DROSHA.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi220480, 2 interactors
ComplexPortaliCPX-3081 Microprocessor complex
CORUMiQ9EQM6
IntActiQ9EQM6, 1 interactor
STRINGi10090.ENSMUSP00000009321

Structurei

3D structure databases

ProteinModelPortaliQ9EQM6
SMRiQ9EQM6
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini301 – 334WWPROSITE-ProRule annotationAdd BLAST34
Domaini511 – 578DRBM 1PROSITE-ProRule annotationAdd BLAST68
Domaini620 – 685DRBM 2PROSITE-ProRule annotationAdd BLAST66

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 342Necessary for interaction with NCLBy similarityAdd BLAST342
Regioni1 – 275Necessary for nuclear localization and retentionBy similarityAdd BLAST275
Regioni276 – 751Necessary for heme-binding and pri-miRNA processingBy similarityAdd BLAST476
Regioni701 – 773Interaction with DROSHABy similarityAdd BLAST73

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4334 Eukaryota
ENOG410XS9I LUCA
GeneTreeiENSGT00390000015977
HOGENOMiHOG000232104
HOVERGENiHBG051344
InParanoidiQ9EQM6
KOiK18419
OMAiETRVVTW
OrthoDBiEOG091G09GA
PhylomeDBiQ9EQM6
TreeFamiTF324256

Family and domain databases

CDDicd00048 DSRM, 1 hit
cd00201 WW, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 2 hits
SM00456 WW, 1 hit
SUPFAMiSSF51045 SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS50020 WW_DOMAIN_2, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9EQM6-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
METYESPSPL PREPAGEAMM ENRACPFQVL PHEQSPPPPL QTSSDAEVMD
60 70 80 90 100
VGSGGDGQSE PPADDPFNFY GASLLSKGSF SKGRLLIDPN CSGHSPRTAR
110 120 130 140 150
HAPAVRKFSP DLKLLKDVKI SVSFTESCRS KDRKVLYTGV ERSTRPECGQ
160 170 180 190 200
LLSPVSGDVH ACPFGGSVGN GVGLGGESAD KKDEENELDQ EKRVEYAVLD
210 220 230 240 250
ELEDFTDNLE LDEEGTGGFT AKAIVQRDRV DEEALNFSYE DDFDNDVDAL
260 270 280 290 300
LEEGLCAPKK RRMEEKYGGD SDHPSDGETS VQPMMTKIKT VLKSRGRPPT
310 320 330 340 350
EPLPDGWIMT FHNSGVPVYL HRESRVVTWS RPYFLGTGSI RKHDPPLSSI
360 370 380 390 400
PCLHYKKMKD NEEREQNCDL APSGEVSPVK PLGRSAELDF PLEEPDSMGG
410 420 430 440 450
DSGSMDEKDP LGAEAAAGAL GQVKAKVEVC KDESVDLEEF RNYLEKRFDF
460 470 480 490 500
EQVTVKKFRT WAERRQFNRE MKRKQAESER PILPANQKLI TLSVQDAPTK
510 520 530 540 550
KEFVINPNGK SEVCILHEYM QRVLKVRPVY NFFECENPSE PFGASVTIDG
560 570 580 590 600
VTYGSGTASS KKLAKNKAAR ATLEILIPDF VKQTSEEKPK DSEELEYFNH
610 620 630 640 650
ISIEDSRVYE LTSKAGLLSP YQILHECLKR NHGMGDTSIK FEVVPGKNQK
660 670 680 690 700
SEYVMACGKH TVRGWCKNKR VGKQLASQKI LQLLHPHVKN WGSLLRMYGR
710 720 730 740 750
ESSKMVKQET SDKSVIELQQ YAKKNRPNLH ILSKLQEEMK RLAAEREETR
760 770
KKPKMSIVAS AQPGGEPLCT VDV
Length:773
Mass (Da):86,321
Last modified:July 19, 2004 - v2
Checksum:iDA837BD6017B0446
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A338P666A0A338P666_MOUSE
Microprocessor complex subunit DGCR...
Dgcr8
141Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti241 – 243DDF → VCW in AAG35599 (Ref. 3) Curated3

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086857 mRNA Translation: BAD06468.1
BC062919 mRNA Translation: AAH62919.1
BC066118 mRNA Translation: AAH66118.1
AF201683 mRNA Translation: AAG35599.1
CCDSiCCDS28018.1
RefSeqiNP_201581.2, NM_033324.2
UniGeneiMm.447352
Mm.482156

Genome annotation databases

EnsembliENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718
ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687
GeneIDi94223
KEGGimmu:94223
UCSCiuc007yne.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AB086857 mRNA Translation: BAD06468.1
BC062919 mRNA Translation: AAH62919.1
BC066118 mRNA Translation: AAH66118.1
AF201683 mRNA Translation: AAG35599.1
CCDSiCCDS28018.1
RefSeqiNP_201581.2, NM_033324.2
UniGeneiMm.447352
Mm.482156

3D structure databases

ProteinModelPortaliQ9EQM6
SMRiQ9EQM6
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi220480, 2 interactors
ComplexPortaliCPX-3081 Microprocessor complex
CORUMiQ9EQM6
IntActiQ9EQM6, 1 interactor
STRINGi10090.ENSMUSP00000009321

PTM databases

iPTMnetiQ9EQM6
PhosphoSitePlusiQ9EQM6

Proteomic databases

EPDiQ9EQM6
MaxQBiQ9EQM6
PaxDbiQ9EQM6
PeptideAtlasiQ9EQM6
PRIDEiQ9EQM6

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718
ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687
GeneIDi94223
KEGGimmu:94223
UCSCiuc007yne.1 mouse

Organism-specific databases

CTDi54487
MGIiMGI:2151114 Dgcr8

Phylogenomic databases

eggNOGiKOG4334 Eukaryota
ENOG410XS9I LUCA
GeneTreeiENSGT00390000015977
HOGENOMiHOG000232104
HOVERGENiHBG051344
InParanoidiQ9EQM6
KOiK18419
OMAiETRVVTW
OrthoDBiEOG091G09GA
PhylomeDBiQ9EQM6
TreeFamiTF324256

Miscellaneous databases

ChiTaRSiDgcr8 mouse
PROiPR:Q9EQM6
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022718 Expressed in 244 organ(s), highest expression level in ear vesicle
GenevisibleiQ9EQM6 MM

Family and domain databases

CDDicd00048 DSRM, 1 hit
cd00201 WW, 1 hit
InterProiView protein in InterPro
IPR014720 dsRBD_dom
IPR001202 WW_dom
IPR036020 WW_dom_sf
PfamiView protein in Pfam
PF00035 dsrm, 2 hits
SMARTiView protein in SMART
SM00358 DSRM, 2 hits
SM00456 WW, 1 hit
SUPFAMiSSF51045 SSF51045, 1 hit
PROSITEiView protein in PROSITE
PS50137 DS_RBD, 1 hit
PS50020 WW_DOMAIN_2, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiDGCR8_MOUSE
AccessioniPrimary (citable) accession number: Q9EQM6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 10, 2002
Last sequence update: July 19, 2004
Last modified: November 7, 2018
This is version 130 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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