UniProtKB - Q9EQM6 (DGCR8_MOUSE)
Microprocessor complex subunit DGCR8
Dgcr8
Functioni
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 352 | Iron (heme axial ligand)By similarity | 1 |
GO - Molecular functioni
- double-stranded RNA binding Source: MGI
- heme binding Source: MGI
- identical protein binding Source: MGI
- metal ion binding Source: UniProtKB-KW
- primary miRNA binding Source: MGI
- protein homodimerization activity Source: MGI
GO - Biological processi
- primary miRNA processing Source: MGI
- regulation of stem cell proliferation Source: MGI
- RNA phosphodiester bond hydrolysis, endonucleolytic Source: GOC
Keywordsi
Molecular function | RNA-binding |
Ligand | Heme, Iron, Metal-binding |
Names & Taxonomyi
Protein namesi | Recommended name: Microprocessor complex subunit DGCR8Alternative name(s): DiGeorge syndrome critical region 8 homolog Gy1 |
Gene namesi | Name:Dgcr8 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2151114, Dgcr8 |
Subcellular locationi
Nucleus
Note: Colocalizes with nucleolin and DROSHA in the nucleolus. Mostly detected in the nucleolus as electron-dense granular patches around the fibrillar center (FC) and granular component (GC). Also detected in the nucleoplasm as small foci adjacent to splicing speckles near the chromatin structure. Localized with DROSHA in GW bodies (GWBs), also known as P-bodies.By similarity
Nucleus
- microprocessor complex Source: MGI
- nucleolus Source: UniProtKB-SubCell
- nucleoplasm Source: Reactome
- nucleus Source: MGI
Other locations
- cytoplasm Source: MGI
- postsynaptic density Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000079879 | 1 – 773 | Microprocessor complex subunit DGCR8Add BLAST | 773 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 35 | PhosphoserineBy similarity | 1 | |
Modified residuei | 92 | PhosphoserineBy similarity | 1 | |
Modified residuei | 95 | PhosphoserineCombined sources | 1 | |
Modified residuei | 271 | PhosphoserineCombined sources | 1 | |
Modified residuei | 275 | PhosphoserineCombined sources | 1 | |
Modified residuei | 279 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 373 | PhosphoserineBy similarity | 1 | |
Modified residuei | 377 | PhosphoserineCombined sources | 1 | |
Cross-linki | 424 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 500 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity | ||
Cross-linki | 707 | Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)By similarity |
Keywords - PTMi
Isopeptide bond, Phosphoprotein, Ubl conjugationProteomic databases
EPDi | Q9EQM6 |
jPOSTi | Q9EQM6 |
MaxQBi | Q9EQM6 |
PaxDbi | Q9EQM6 |
PeptideAtlasi | Q9EQM6 |
PRIDEi | Q9EQM6 |
PTM databases
iPTMneti | Q9EQM6 |
PhosphoSitePlusi | Q9EQM6 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000022718, Expressed in humerus cartilage element and 262 other tissues |
ExpressionAtlasi | Q9EQM6, baseline and differential |
Genevisiblei | Q9EQM6, MM |
Interactioni
Subunit structurei
Monomer; in absence of heme (By similarity). Homodimer; the association with heme promotes its dimerization (By similarity).
Component of the microprocessor complex, or pri-miRNA processing protein complex, which is composed of DROSHA and DGCR8 (PubMed:26255770). The microprocessor complex is a heterotrimer; each of the two DROSHA RNase III domains binds one DGCR8 (via C-terminal region) (By similarity).
Interacts with ILF3, NCL and DROSHA (By similarity).
Interacts with CPSF3 and ISY1; this interaction is in an RNA dependent manner (PubMed:26255770).
Interacts with PUS10; interaction promotes pri-miRNAs processing (By similarity).
By similarity1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
- protein homodimerization activity Source: MGI
Protein-protein interaction databases
BioGRIDi | 220480, 7 interactors |
ComplexPortali | CPX-3081, Microprocessor complex |
CORUMi | Q9EQM6 |
IntActi | Q9EQM6, 1 interactor |
STRINGi | 10090.ENSMUSP00000009321 |
Miscellaneous databases
RNActi | Q9EQM6, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 301 – 334 | WWPROSITE-ProRule annotationAdd BLAST | 34 | |
Domaini | 511 – 578 | DRBM 1PROSITE-ProRule annotationAdd BLAST | 68 | |
Domaini | 620 – 685 | DRBM 2PROSITE-ProRule annotationAdd BLAST | 66 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 342 | Necessary for interaction with NCLBy similarityAdd BLAST | 342 | |
Regioni | 1 – 275 | Necessary for nuclear localization and retentionBy similarityAdd BLAST | 275 | |
Regioni | 276 – 751 | Necessary for heme-binding and pri-miRNA processingBy similarityAdd BLAST | 476 | |
Regioni | 701 – 773 | Interaction with DROSHABy similarityAdd BLAST | 73 |
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG4334, Eukaryota |
GeneTreei | ENSGT00390000015977 |
HOGENOMi | CLU_017211_3_0_1 |
InParanoidi | Q9EQM6 |
OMAi | MMENRAC |
OrthoDBi | 855874at2759 |
PhylomeDBi | Q9EQM6 |
TreeFami | TF324256 |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd00201, WW, 1 hit |
InterProi | View protein in InterPro IPR040375, DGCR8 IPR014720, dsRBD_dom IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR13482, PTHR13482, 1 hit |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
METYESPSPL PREPAGEAMM ENRACPFQVL PHEQSPPPPL QTSSDAEVMD
60 70 80 90 100
VGSGGDGQSE PPADDPFNFY GASLLSKGSF SKGRLLIDPN CSGHSPRTAR
110 120 130 140 150
HAPAVRKFSP DLKLLKDVKI SVSFTESCRS KDRKVLYTGV ERSTRPECGQ
160 170 180 190 200
LLSPVSGDVH ACPFGGSVGN GVGLGGESAD KKDEENELDQ EKRVEYAVLD
210 220 230 240 250
ELEDFTDNLE LDEEGTGGFT AKAIVQRDRV DEEALNFSYE DDFDNDVDAL
260 270 280 290 300
LEEGLCAPKK RRMEEKYGGD SDHPSDGETS VQPMMTKIKT VLKSRGRPPT
310 320 330 340 350
EPLPDGWIMT FHNSGVPVYL HRESRVVTWS RPYFLGTGSI RKHDPPLSSI
360 370 380 390 400
PCLHYKKMKD NEEREQNCDL APSGEVSPVK PLGRSAELDF PLEEPDSMGG
410 420 430 440 450
DSGSMDEKDP LGAEAAAGAL GQVKAKVEVC KDESVDLEEF RNYLEKRFDF
460 470 480 490 500
EQVTVKKFRT WAERRQFNRE MKRKQAESER PILPANQKLI TLSVQDAPTK
510 520 530 540 550
KEFVINPNGK SEVCILHEYM QRVLKVRPVY NFFECENPSE PFGASVTIDG
560 570 580 590 600
VTYGSGTASS KKLAKNKAAR ATLEILIPDF VKQTSEEKPK DSEELEYFNH
610 620 630 640 650
ISIEDSRVYE LTSKAGLLSP YQILHECLKR NHGMGDTSIK FEVVPGKNQK
660 670 680 690 700
SEYVMACGKH TVRGWCKNKR VGKQLASQKI LQLLHPHVKN WGSLLRMYGR
710 720 730 740 750
ESSKMVKQET SDKSVIELQQ YAKKNRPNLH ILSKLQEEMK RLAAEREETR
760 770
KKPKMSIVAS AQPGGEPLCT VDV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A338P666 | A0A338P666_MOUSE | Microprocessor complex subunit DGCR... | Dgcr8 | 141 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 241 – 243 | DDF → VCW in AAG35599 (Ref. 3) Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB086857 mRNA Translation: BAD06468.1 BC062919 mRNA Translation: AAH62919.1 BC066118 mRNA Translation: AAH66118.1 AF201683 mRNA Translation: AAG35599.1 |
CCDSi | CCDS28018.1 |
RefSeqi | NP_201581.2, NM_033324.2 |
Genome annotation databases
Ensembli | ENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718 ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687 |
GeneIDi | 94223 |
KEGGi | mmu:94223 |
UCSCi | uc007yne.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB086857 mRNA Translation: BAD06468.1 BC062919 mRNA Translation: AAH62919.1 BC066118 mRNA Translation: AAH66118.1 AF201683 mRNA Translation: AAG35599.1 |
CCDSi | CCDS28018.1 |
RefSeqi | NP_201581.2, NM_033324.2 |
3D structure databases
BMRBi | Q9EQM6 |
SMRi | Q9EQM6 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 220480, 7 interactors |
ComplexPortali | CPX-3081, Microprocessor complex |
CORUMi | Q9EQM6 |
IntActi | Q9EQM6, 1 interactor |
STRINGi | 10090.ENSMUSP00000009321 |
PTM databases
iPTMneti | Q9EQM6 |
PhosphoSitePlusi | Q9EQM6 |
Proteomic databases
EPDi | Q9EQM6 |
jPOSTi | Q9EQM6 |
MaxQBi | Q9EQM6 |
PaxDbi | Q9EQM6 |
PeptideAtlasi | Q9EQM6 |
PRIDEi | Q9EQM6 |
Genome annotation databases
Ensembli | ENSMUST00000009321; ENSMUSP00000009321; ENSMUSG00000022718 ENSMUST00000202260; ENSMUSP00000144454; ENSMUSG00000106687 |
GeneIDi | 94223 |
KEGGi | mmu:94223 |
UCSCi | uc007yne.1, mouse |
Organism-specific databases
CTDi | 54487 |
MGIi | MGI:2151114, Dgcr8 |
Phylogenomic databases
eggNOGi | KOG4334, Eukaryota |
GeneTreei | ENSGT00390000015977 |
HOGENOMi | CLU_017211_3_0_1 |
InParanoidi | Q9EQM6 |
OMAi | MMENRAC |
OrthoDBi | 855874at2759 |
PhylomeDBi | Q9EQM6 |
TreeFami | TF324256 |
Miscellaneous databases
BioGRID-ORCSi | 94223, 6 hits in 18 CRISPR screens |
ChiTaRSi | Dgcr8, mouse |
PROi | PR:Q9EQM6 |
RNActi | Q9EQM6, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000022718, Expressed in humerus cartilage element and 262 other tissues |
ExpressionAtlasi | Q9EQM6, baseline and differential |
Genevisiblei | Q9EQM6, MM |
Family and domain databases
CDDi | cd00048, DSRM, 1 hit cd00201, WW, 1 hit |
InterProi | View protein in InterPro IPR040375, DGCR8 IPR014720, dsRBD_dom IPR001202, WW_dom IPR036020, WW_dom_sf |
PANTHERi | PTHR13482, PTHR13482, 1 hit |
Pfami | View protein in Pfam PF00035, dsrm, 2 hits |
SMARTi | View protein in SMART SM00358, DSRM, 2 hits SM00456, WW, 1 hit |
SUPFAMi | SSF51045, SSF51045, 1 hit |
PROSITEi | View protein in PROSITE PS50137, DS_RBD, 1 hit PS50020, WW_DOMAIN_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | DGCR8_MOUSE | |
Accessioni | Q9EQM6Primary (citable) accession number: Q9EQM6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 10, 2002 |
Last sequence update: | July 19, 2004 | |
Last modified: | February 10, 2021 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot