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UniProtKB - Q9EQK7 (ICMT_MOUSE)
Protein
Protein-S-isoprenylcysteine O-methyltransferase
Gene
Icmt
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the post-translational methylation of isoprenylated C-terminal cysteine residues.
By similarityCatalytic activityi
- [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine + S-adenosyl-L-methionine = [protein]-C-terminal S-[(2E,6E)-farnesyl]-L-cysteine methyl ester + S-adenosyl-L-homocysteineBy similarityEC:2.1.1.100By similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 189 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 204 | S-adenosyl-L-methionineBy similarity | 1 | |
Binding sitei | 246 | SubstrateBy similarity | 1 | |
Binding sitei | 250 | S-adenosyl-L-methionineBy similarity | 1 |
GO - Molecular functioni
- cAMP response element binding protein binding Source: MGI
- protein C-terminal S-isoprenylcysteine carboxyl O-methyltransferase activity Source: MGI
GO - Biological processi
- bone mineralization Source: MGI
- C-terminal protein methylation Source: MGI
- determination of adult lifespan Source: MGI
- in utero embryonic development Source: MGI
- liver development Source: MGI
- multicellular organism growth Source: MGI
- neuromuscular process Source: MGI
- positive regulation of cell population proliferation Source: MGI
- protein localization Source: MGI
- protein methylation Source: MGI
- regulation of cellular senescence Source: MGI
- regulation of protein localization Source: MGI
- regulation of Ras protein signal transduction Source: MGI
- S-adenosylhomocysteine metabolic process Source: MGI
- S-adenosylmethioninamine metabolic process Source: MGI
- TOR signaling Source: MGI
Keywordsi
Molecular function | Methyltransferase, Transferase |
Ligand | S-adenosyl-L-methionine |
Enzyme and pathway databases
BRENDAi | 2.1.1.100, 3474 |
Reactomei | R-MMU-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation R-MMU-9648002, RAS processing |
SABIO-RKi | Q9EQK7 |
Names & Taxonomyi
Protein namesi | Recommended name: Protein-S-isoprenylcysteine O-methyltransferase (EC:2.1.1.100By similarity)Alternative name(s): Isoprenylcysteine carboxylmethyltransferase Prenylated protein carboxyl methyltransferase Short name: PPMT Prenylcysteine carboxyl methyltransferase Short name: pcCMT |
Gene namesi | Name:Icmt |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1888594, Icmt |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane By similarity; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum Source: MGI
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 15 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 16 – 32 | HelicalSequence analysisAdd BLAST | 17 | |
Topological domaini | 33 – 40 | LumenalSequence analysis | 8 | |
Transmembranei | 41 – 58 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 59 – 68 | CytoplasmicSequence analysis | 10 | |
Transmembranei | 69 – 86 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 87 – 91 | LumenalSequence analysis | 5 | |
Transmembranei | 92 – 111 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 112 – 130 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 131 – 148 | HelicalSequence analysisAdd BLAST | 18 | |
Topological domaini | 149 – 153 | LumenalSequence analysis | 5 | |
Transmembranei | 154 – 173 | HelicalSequence analysisAdd BLAST | 20 | |
Topological domaini | 174 – 211 | CytoplasmicSequence analysisAdd BLAST | 38 | |
Transmembranei | 212 – 227 | HelicalSequence analysisAdd BLAST | 16 | |
Topological domaini | 228 | LumenalSequence analysis | 1 | |
Transmembranei | 229 – 243 | HelicalSequence analysisAdd BLAST | 15 | |
Topological domaini | 244 – 283 | CytoplasmicSequence analysisAdd BLAST | 40 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000209895 | 1 – 283 | Protein-S-isoprenylcysteine O-methyltransferaseAdd BLAST | 283 |
Proteomic databases
MaxQBi | Q9EQK7 |
PaxDbi | Q9EQK7 |
PRIDEi | Q9EQK7 |
ProteomicsDBi | 267089 |
PTM databases
iPTMneti | Q9EQK7 |
PhosphoSitePlusi | Q9EQK7 |
Expressioni
Tissue specificityi
Highly enriched in adult cerebellum, with a low level expression in other brain regions.
Developmental stagei
During cerebellar development expression was low before postnatal day P6 and dramatically increased after P12.
Interactioni
GO - Molecular functioni
- cAMP response element binding protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 208249, 3 interactors |
STRINGi | 10090.ENSMUSP00000043390 |
Miscellaneous databases
RNActi | Q9EQK7, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 196 – 199 | S-adenosyl-L-methionine bindingBy similarity | 4 | |
Regioni | 209 – 212 | S-adenosyl-L-methionine bindingBy similarity | 4 |
Sequence similaritiesi
Belongs to the class VI-like SAM-binding methyltransferase superfamily. Isoprenylcysteine carboxyl methyltransferase family.PROSITE-ProRule annotation
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2628, Eukaryota |
InParanoidi | Q9EQK7 |
OrthoDBi | 1479599at2759 |
PhylomeDBi | Q9EQK7 |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9EQK7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MAAARRGSAG SEARLSLATF LLGASVLALP LLTRAGLQGR TGLALYVAGL
60 70 80 90 100
NALLLLLYRP PRYQIAIRAC FLGFVFGCGV LLSFSQSSWN HFGWYVCSLS
110 120 130 140 150
LFHYSEYLVT AVNNPKSLSL DSFLLNHSLE YTVAALSSWI EFTLENIFWP
160 170 180 190 200
ELKQITWLSA TGLLMVVFGE CLRKAAMFTA GSNFNHVVQS EKSDTHTLVT
210 220 230 240 250
SGVYAWCRHP SYVGWFYWSI GTQVMLCNPI CGVVYALTVW RFFRDRTEEE
260 270 280
EISLIHFFGE EYLDYKKRVP TGLPFIKGVK VEL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3U4N2 | Q3U4N2_MOUSE | Protein-S-isoprenylcysteine O-methy... | Icmt | 284 | Annotation score: | ||
Q6PGA6 | Q6PGA6_MOUSE | Icmt protein | Icmt | 105 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 24 | A → D in AA022288 (Ref. 2) Curated | 1 | |
Sequence conflicti | 42 | G → A (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF209926 mRNA Translation: AAG48552.1 AA022288 mRNA No translation available. |
RefSeqi | NP_598549.1, NM_133788.2 |
Genome annotation databases
GeneIDi | 57295 |
KEGGi | mmu:57295 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF209926 mRNA Translation: AAG48552.1 AA022288 mRNA No translation available. |
RefSeqi | NP_598549.1, NM_133788.2 |
3D structure databases
AlphaFoldDBi | Q9EQK7 |
SMRi | Q9EQK7 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208249, 3 interactors |
STRINGi | 10090.ENSMUSP00000043390 |
PTM databases
iPTMneti | Q9EQK7 |
PhosphoSitePlusi | Q9EQK7 |
Proteomic databases
MaxQBi | Q9EQK7 |
PaxDbi | Q9EQK7 |
PRIDEi | Q9EQK7 |
ProteomicsDBi | 267089 |
Protocols and materials databases
DNASUi | 57295 |
Genome annotation databases
GeneIDi | 57295 |
KEGGi | mmu:57295 |
Organism-specific databases
CTDi | 23463 |
MGIi | MGI:1888594, Icmt |
Phylogenomic databases
eggNOGi | KOG2628, Eukaryota |
InParanoidi | Q9EQK7 |
OrthoDBi | 1479599at2759 |
PhylomeDBi | Q9EQK7 |
Enzyme and pathway databases
BRENDAi | 2.1.1.100, 3474 |
Reactomei | R-MMU-163841, Gamma carboxylation, hypusine formation and arylsulfatase activation R-MMU-9648002, RAS processing |
SABIO-RKi | Q9EQK7 |
Miscellaneous databases
BioGRID-ORCSi | 57295, 14 hits in 74 CRISPR screens |
PROi | PR:Q9EQK7 |
RNActi | Q9EQK7, protein |
SOURCEi | Search... |
Family and domain databases
InterProi | View protein in InterPro IPR007269, ICMT_MeTrfase IPR025770, PPMT_MeTrfase |
Pfami | View protein in Pfam PF04140, ICMT, 1 hit |
PROSITEi | View protein in PROSITE PS51564, SAM_ICMT, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ICMT_MOUSE | |
Accessioni | Q9EQK7Primary (citable) accession number: Q9EQK7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | June 20, 2001 |
Last sequence update: | June 20, 2001 | |
Last modified: | May 25, 2022 | |
This is version 130 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families