UniProtKB - Q9EQH5 (CTBP2_RAT)
C-terminal-binding protein 2
Ctbp2
Functioni
Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation. Isoform 2 probably acts as a scaffold for specialized synapses (By similarity).
By similaritySites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 106 | NADBy similarity | 1 | |
Binding sitei | 210 | NADBy similarity | 1 | |
Active sitei | 272 | By similarity | 1 | |
Binding sitei | 296 | NADBy similarity | 1 | |
Active sitei | 301 | By similarity | 1 | |
Active sitei | 321 | Proton donorBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 186 – 191 | NADBy similarity | 6 | |
Nucleotide bindingi | 243 – 249 | NADBy similarity | 7 | |
Nucleotide bindingi | 270 – 272 | NADBy similarity | 3 | |
Nucleotide bindingi | 321 – 324 | NADBy similarity | 4 |
GO - Molecular functioni
- chromatin binding Source: RGD
- NAD binding Source: InterPro
- oxidoreductase activity, acting on the CH-OH group of donors, NAD or NADP as acceptor Source: InterPro
- protein-containing complex binding Source: RGD
- protein kinase binding Source: RGD
- retinoic acid receptor binding Source: RGD
- structural constituent of presynaptic active zone Source: RGD
- transcription coactivator activity Source: RGD
- transcription coregulator binding Source: RGD
- transcription corepressor activity Source: RGD
- transcription corepressor binding Source: RGD
GO - Biological processi
- cellular response to leukemia inhibitory factor Source: RGD
- negative regulation of transcription, DNA-templated Source: UniProtKB
- positive regulation of chromatin binding Source: RGD
- positive regulation of retinoic acid receptor signaling pathway Source: RGD
- positive regulation of transcription by RNA polymerase II Source: RGD
- synaptic vesicle docking Source: RGD
- white fat cell differentiation Source: UniProtKB
Keywordsi
Molecular function | Oxidoreductase, Repressor |
Biological process | Differentiation, Transcription, Transcription regulation |
Ligand | NAD |
Enzyme and pathway databases
Reactomei | R-RNO-4641265, Repression of WNT target genes |
Names & Taxonomyi
Protein namesi | Recommended name: C-terminal-binding protein 2Short name: CtBP2 |
Gene namesi | Name:Ctbp2 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 68372, Ctbp2 |
Subcellular locationi
Cytosol
- presynaptic cytosol Source: RGD
Nucleus
- nucleus Source: UniProtKB
Other locations
- GABA-ergic synapse Source: RGD
- glutamatergic synapse Source: RGD
- photoreceptor ribbon synapse Source: RGD
- presynaptic active zone cytoplasmic component Source: RGD
- ribbon synapse Source: RGD
- synapse Source: RGD
- transcription repressor complex Source: UniProtKB
Keywords - Cellular componenti
Cell junction, Nucleus, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000299356 | 1 – 445 | C-terminal-binding protein 2Add BLAST | 445 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 22 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 428 | Phosphoserine; by HIPK2By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
jPOSTi | Q9EQH5 |
PaxDbi | Q9EQH5 |
PRIDEi | Q9EQH5 |
PTM databases
iPTMneti | Q9EQH5 |
PhosphoSitePlusi | Q9EQH5 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSRNOG00000017326, Expressed in testis and 21 other tissues |
Genevisiblei | Q9EQH5, RN |
Interactioni
Subunit structurei
Interacts with HIPK2, ZNF217 and PNN (By similarity).
Interacts with the transcription factors BKLF, delta EF1/AREB6/ZEB, EVI-1 and Friend of GATA (FOG) via the consensus motif P-X-[DNS]-L-[STVA]. Can form a complex with BKLF on a CACCC-box oligonucleotide. Can form homodimers or heterodimers of CTBP1 and CTBP2.
Interacts with NRIP1 and WIZ.
Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression (By similarity).
Interacts with MCRIP1 (By similarity).
By similarityGO - Molecular functioni
- protein kinase binding Source: RGD
- retinoic acid receptor binding Source: RGD
- transcription coregulator binding Source: RGD
- transcription corepressor binding Source: RGD
Protein-protein interaction databases
BioGRIDi | 249593, 1 interactor |
STRINGi | 10116.ENSRNOP00000023404 |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 414 – 445 | DisorderedSequence analysisAdd BLAST | 32 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 418 – 433 | Polar residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0067, Eukaryota |
GeneTreei | ENSGT00940000154430 |
HOGENOMi | CLU_012460_0_0_1 |
InParanoidi | Q9EQH5 |
OMAi | MLAPEPX |
OrthoDBi | 700058at2759 |
PhylomeDBi | Q9EQH5 |
TreeFami | TF313593 |
Family and domain databases
CDDi | cd05299, CtBP_dh, 1 hit |
InterProi | View protein in InterPro IPR043322, CtBP IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MALVDKHKVK RQRLDRICEG IRPQIMNGPL HPRPLVALLD GRDCTVEMPI
60 70 80 90 100
LKDLATVAFC DAQSTQEIHE KVLNEAVGAM MYHTITLTRE DLEKFKALRV
110 120 130 140 150
IVRIGSGYDN VDIKAAGELG IAVCNIPSAA VEETADSTVC HILNLYRRNT
160 170 180 190 200
WLYQALREGT RVQSVEQIRE VASGAARIRG ETLGLIGFGR TGQAVAVRAK
210 220 230 240 250
AFGFSVIFYD PYLQDGIERS LGVQRVYTLQ DLLYQSDCVS LHCNLNEHNH
260 270 280 290 300
HLINDFTIKQ MRQGAFLVNA ARGGLVDEKA LAQALKEGRI RGAALDVHES
310 320 330 340 350
EPFSFAQGPL KDAPNLICTP HTAWYSEQAS LEMREAAATE IRRAITGRIP
360 370 380 390 400
ESLRNCVNKE FFVTSTPWSV IDQQAIHPEL NGATYRYPPG IVGVAPGGLP
410 420 430 440
PAMEGIIPGG IPVTHNLPTV AHPSQAPSPN QPTKHGDNRE HPNEQ
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A8I6AEI5 | A0A8I6AEI5_RAT | C-terminal-binding protein 2 | Ctbp2 | 406 | Annotation score: | ||
A0A8I6ALN3 | A0A8I6ALN3_RAT | C-terminal-binding protein 2 | Ctbp2 | 432 | Annotation score: |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_027611 | 1 – 20 | MALVD…RICEG → MPVPSRHINIGRSQSWDAAG WYEGPWENAGPPGRRSSLTY GPGEGVWCELVNHRAQDTES CLSREAFYNSLASRKGSVPD FTFYDSRQAVMSGRGSVLPQ DYYGDPSRGTRVPKEPPFYR DPGTSRPVPSYGVLGSRIPW EQVQGQLPALQDAGHLYRES GSKTVLHGQRTHCRAPSPGR YGREQPDSRLGIEVPTYSPN SSQVYNDICERPVDSTHARQ VAPTCLVVDPSSTAPTENST GVAPGSLNRGYGPTRESIHS KLAYENYEADLSTFQGPGGK RTVYPEFLALLRAEGVAEAT LAALLQQGFDSPAVLATLED ADIKSVAPNLGQARVLSRLV SSCRTEMQFRRQDRTGPPPR HRSSSFSHRSELLPNDTASL GTTALQFHPAGPLQTPSPRG GDLGRRPSSAPSQHLLETAA TYSAPVVGSQTPHLPSNSGY SSPTPCALTARLASSYPSQA GVALTANPGPSVPLHSSPRT AYSTSYTVPMELLKRERSVT ASPLPSPHASPQLLRKPGAA PVEPAALPPVRQSLHTPHPP YQKVARRTGAPIIVSTMLTP EPS in isoform 2. 1 PublicationAdd BLAST | 20 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222712 mRNA Translation: AAG45952.1 AABR03001613 Genomic DNA No translation available. AABR03000195 Genomic DNA No translation available. |
RefSeqi | NP_445787.1, NM_053335.1 [Q9EQH5-2] |
Genome annotation databases
Ensembli | ENSRNOT00000023404; ENSRNOP00000023404; ENSRNOG00000017326 [Q9EQH5-2] ENSRNOT00000114061; ENSRNOP00000090411; ENSRNOG00000017326 [Q9EQH5-1] |
GeneIDi | 81717 |
KEGGi | rno:81717 |
UCSCi | RGD:68372, rat [Q9EQH5-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222712 mRNA Translation: AAG45952.1 AABR03001613 Genomic DNA No translation available. AABR03000195 Genomic DNA No translation available. |
RefSeqi | NP_445787.1, NM_053335.1 [Q9EQH5-2] |
3D structure databases
AlphaFoldDBi | Q9EQH5 |
SMRi | Q9EQH5 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 249593, 1 interactor |
STRINGi | 10116.ENSRNOP00000023404 |
PTM databases
iPTMneti | Q9EQH5 |
PhosphoSitePlusi | Q9EQH5 |
Proteomic databases
jPOSTi | Q9EQH5 |
PaxDbi | Q9EQH5 |
PRIDEi | Q9EQH5 |
Genome annotation databases
Ensembli | ENSRNOT00000023404; ENSRNOP00000023404; ENSRNOG00000017326 [Q9EQH5-2] ENSRNOT00000114061; ENSRNOP00000090411; ENSRNOG00000017326 [Q9EQH5-1] |
GeneIDi | 81717 |
KEGGi | rno:81717 |
UCSCi | RGD:68372, rat [Q9EQH5-1] |
Organism-specific databases
CTDi | 1488 |
RGDi | 68372, Ctbp2 |
Phylogenomic databases
eggNOGi | KOG0067, Eukaryota |
GeneTreei | ENSGT00940000154430 |
HOGENOMi | CLU_012460_0_0_1 |
InParanoidi | Q9EQH5 |
OMAi | MLAPEPX |
OrthoDBi | 700058at2759 |
PhylomeDBi | Q9EQH5 |
TreeFami | TF313593 |
Enzyme and pathway databases
Reactomei | R-RNO-4641265, Repression of WNT target genes |
Miscellaneous databases
PROi | PR:Q9EQH5 |
Gene expression databases
Bgeei | ENSRNOG00000017326, Expressed in testis and 21 other tissues |
Genevisiblei | Q9EQH5, RN |
Family and domain databases
CDDi | cd05299, CtBP_dh, 1 hit |
InterProi | View protein in InterPro IPR043322, CtBP IPR006139, D-isomer_2_OHA_DH_cat_dom IPR029753, D-isomer_DH_CS IPR006140, D-isomer_DH_NAD-bd IPR036291, NAD(P)-bd_dom_sf |
Pfami | View protein in Pfam PF00389, 2-Hacid_dh, 1 hit PF02826, 2-Hacid_dh_C, 1 hit |
SUPFAMi | SSF51735, SSF51735, 1 hit |
PROSITEi | View protein in PROSITE PS00671, D_2_HYDROXYACID_DH_3, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | CTBP2_RAT | |
Accessioni | Q9EQH5Primary (citable) accession number: Q9EQH5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 11, 2007 |
Last sequence update: | September 11, 2007 | |
Last modified: | May 25, 2022 | |
This is version 137 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families