UniProtKB - Q9EQH4 (TAF8_MOUSE)
Transcription initiation factor TFIID subunit 8
Taf8
Functioni
Miscellaneous
GO - Molecular functioni
- protein heterodimerization activity Source: InterPro
GO - Biological processi
- cell differentiation Source: UniProtKB-KW
- DNA-templated transcription open complex formation Source: MGI
- inner cell mass cell proliferation Source: MGI
- maintenance of protein location in nucleus Source: MGI
- regulation of fat cell differentiation Source: MGI
- transcription initiation from RNA polymerase II promoter Source: GO_Central
Keywordsi
Molecular function | Developmental protein |
Biological process | Differentiation, Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-MMU-6807505, RNA polymerase II transcribes snRNA genes |
Names & Taxonomyi
Protein namesi | Recommended name: Transcription initiation factor TFIID subunit 8Alternative name(s): Protein taube nuss TBP-associated factor 8 |
Gene namesi | Name:Taf8 Synonyms:Tbn |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926879, Taf8 |
Subcellular locationi
Nucleus
- nucleoplasm Source: MGI
- nucleus Source: MGI
- transcription factor TFIID complex Source: UniProtKB
Other locations
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000315398 | 2 – 308 | Transcription initiation factor TFIID subunit 8Add BLAST | 307 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 128 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 269 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
PaxDbi | Q9EQH4 |
PeptideAtlasi | Q9EQH4 |
PRIDEi | Q9EQH4 |
PTM databases
iPTMneti | Q9EQH4 |
PhosphoSitePlusi | Q9EQH4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000023980, Expressed in secondary oocyte and 279 other tissues |
Genevisiblei | Q9EQH4, MM |
Interactioni
Subunit structurei
TFIID is composed of TATA binding protein (TBP) and a number of TBP-associated factors (TAFs).
Interacts with TBP, TAF1, TAF6, TAF10, TAF11 and TAF13. Component also of a small TAF complex (SMAT) containing TAF8, TAF10 and SUPT7L.
Forms a heterodimer with TAF10. Interaction with TAF10 is mediated mainly via its histone fold domain while interaction with SUPT7L is via its C-terminal region (By similarity).
By similarityGO - Molecular functioni
- protein heterodimerization activity Source: InterPro
Protein-protein interaction databases
ComplexPortali | CPX-920, SAGA complex CPX-932, General transcription factor complex TFIID CPX-959, General transcription factor complex TFIID, Taf4b variant |
CORUMi | Q9EQH4 |
STRINGi | 10090.ENSMUSP00000063201 |
Miscellaneous databases
RNActi | Q9EQH4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 35 – 102 | Histone-foldAdd BLAST | 68 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 292 – 305 | Nuclear localization signalSequence analysisAdd BLAST | 14 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG4336, Eukaryota |
GeneTreei | ENSGT00390000017567 |
HOGENOMi | CLU_070829_0_0_1 |
InParanoidi | Q9EQH4 |
OMAi | FPTEDNM |
OrthoDBi | 1290064at2759 |
PhylomeDBi | Q9EQH4 |
TreeFami | TF316311 |
Family and domain databases
CDDi | cd08049, TAF8, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR006565, BTP IPR009072, Histone-fold IPR037818, TAF8 IPR019473, TFIID_su8_C |
PANTHERi | PTHR46469, PTHR46469, 1 hit |
Pfami | View protein in Pfam PF07524, Bromo_TP, 1 hit PF10406, TAF8_C, 1 hit |
SMARTi | View protein in SMART SM00576, BTP, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
s (5)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry describes 5 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MADTAAGPGG SGTRPGSKQS TNPADNYHLA RRRTLQVVVS SLLTEAGFES
60 70 80 90 100
AEKASVETLT EMLQSYISEI GRSAKSYCEH TARTQPTLSD IVVTLVEMGF
110 120 130 140 150
NVDTLPAYAK RSQRMVITAP PVTNQPVTPK ALTAGQNRPH PPHIPSHFPE
160 170 180 190 200
FPDPHTYIKT PTYREPVSDY QILREKAASQ RRDVERALTR FMAKTGETQS
210 220 230 240 250
LFKDDVSTFP LIAARPFTIP YLTALLPSEL EIQQMEETDS SEQEEQTDTE
260 270 280 290 300
NNALHISTDD SGAEKESASV LQQSSSLSGS RNGEESVIDN PYLRPVKKPK
IRRKKSLS
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 127 | V → L in BAC36363 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 153 | D → V in BAC34001 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 236 | E → G in BAC34001 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_030550 | 213 – 221 | AARPFTIPY → SDQHLIKAP in isoform 5. 1 Publication | 9 | |
Alternative sequenceiVSP_030551 | 222 – 308 | Missing in isoform 5. 1 PublicationAdd BLAST | 87 | |
Alternative sequenceiVSP_030552 | 259 – 308 | DDSGA…KKSLS → VGPPLLPASQQSQNSLSHIL PLPLGTEQGLPQVSGWLLQA CMMGCVSSLHHETHLLGSHT FSRW in isoform 4. 1 PublicationAdd BLAST | 50 | |
Alternative sequenceiVSP_030553 | 259 – 265 | DDSGAEK → GHPEASA in isoform 3. 1 Publication | 7 | |
Alternative sequenceiVSP_030554 | 266 – 308 | Missing in isoform 3. 1 PublicationAdd BLAST | 43 | |
Alternative sequenceiVSP_030555 | 306 – 308 | Missing in isoform 2. 1 Publication | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222802 mRNA Translation: AAG01682.1 AK045108 mRNA Translation: BAC32225.1 AK049949 mRNA Translation: BAC34001.1 AK076486 mRNA Translation: BAC36363.1 AK086656 mRNA Translation: BAC39712.1 AK162110 mRNA Translation: BAE36731.1 BC057895 mRNA Translation: AAH57895.1 |
CCDSi | CCDS37642.1 [Q9EQH4-1] CCDS89109.1 [Q9EQH4-3] |
RefSeqi | NP_071298.1, NM_022015.3 [Q9EQH4-1] XP_006524827.1, XM_006524764.2 XP_006524828.1, XM_006524765.3 XP_006524830.1, XM_006524767.3 |
Genome annotation databases
Ensembli | ENSMUST00000067103; ENSMUSP00000063201; ENSMUSG00000023980 [Q9EQH4-1] ENSMUST00000233121; ENSMUSP00000156629; ENSMUSG00000023980 [Q9EQH4-2] ENSMUST00000233174; ENSMUSP00000156668; ENSMUSG00000023980 [Q9EQH4-3] |
GeneIDi | 63856 |
KEGGi | mmu:63856 |
UCSCi | uc008cvh.1, mouse [Q9EQH4-1] uc008cvj.1, mouse [Q9EQH4-4] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF222802 mRNA Translation: AAG01682.1 AK045108 mRNA Translation: BAC32225.1 AK049949 mRNA Translation: BAC34001.1 AK076486 mRNA Translation: BAC36363.1 AK086656 mRNA Translation: BAC39712.1 AK162110 mRNA Translation: BAE36731.1 BC057895 mRNA Translation: AAH57895.1 |
CCDSi | CCDS37642.1 [Q9EQH4-1] CCDS89109.1 [Q9EQH4-3] |
RefSeqi | NP_071298.1, NM_022015.3 [Q9EQH4-1] XP_006524827.1, XM_006524764.2 XP_006524828.1, XM_006524765.3 XP_006524830.1, XM_006524767.3 |
3D structure databases
ModBasei | Search... |
SWISS-MODEL-Workspacei | Submit a new modelling project... |
Protein-protein interaction databases
ComplexPortali | CPX-920, SAGA complex CPX-932, General transcription factor complex TFIID CPX-959, General transcription factor complex TFIID, Taf4b variant |
CORUMi | Q9EQH4 |
STRINGi | 10090.ENSMUSP00000063201 |
PTM databases
iPTMneti | Q9EQH4 |
PhosphoSitePlusi | Q9EQH4 |
Proteomic databases
PaxDbi | Q9EQH4 |
PeptideAtlasi | Q9EQH4 |
PRIDEi | Q9EQH4 |
Protocols and materials databases
Antibodypediai | 30123, 135 antibodies |
DNASUi | 63856 |
Genome annotation databases
Ensembli | ENSMUST00000067103; ENSMUSP00000063201; ENSMUSG00000023980 [Q9EQH4-1] ENSMUST00000233121; ENSMUSP00000156629; ENSMUSG00000023980 [Q9EQH4-2] ENSMUST00000233174; ENSMUSP00000156668; ENSMUSG00000023980 [Q9EQH4-3] |
GeneIDi | 63856 |
KEGGi | mmu:63856 |
UCSCi | uc008cvh.1, mouse [Q9EQH4-1] uc008cvj.1, mouse [Q9EQH4-4] |
Organism-specific databases
CTDi | 129685 |
MGIi | MGI:1926879, Taf8 |
Phylogenomic databases
eggNOGi | KOG4336, Eukaryota |
GeneTreei | ENSGT00390000017567 |
HOGENOMi | CLU_070829_0_0_1 |
InParanoidi | Q9EQH4 |
OMAi | FPTEDNM |
OrthoDBi | 1290064at2759 |
PhylomeDBi | Q9EQH4 |
TreeFami | TF316311 |
Enzyme and pathway databases
Reactomei | R-MMU-6807505, RNA polymerase II transcribes snRNA genes |
Miscellaneous databases
BioGRID-ORCSi | 63856, 10 hits in 19 CRISPR screens |
ChiTaRSi | Taf8, mouse |
PROi | PR:Q9EQH4 |
RNActi | Q9EQH4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023980, Expressed in secondary oocyte and 279 other tissues |
Genevisiblei | Q9EQH4, MM |
Family and domain databases
CDDi | cd08049, TAF8, 1 hit |
Gene3Di | 1.10.20.10, 1 hit |
InterProi | View protein in InterPro IPR006565, BTP IPR009072, Histone-fold IPR037818, TAF8 IPR019473, TFIID_su8_C |
PANTHERi | PTHR46469, PTHR46469, 1 hit |
Pfami | View protein in Pfam PF07524, Bromo_TP, 1 hit PF10406, TAF8_C, 1 hit |
SMARTi | View protein in SMART SM00576, BTP, 1 hit |
SUPFAMi | SSF47113, SSF47113, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | TAF8_MOUSE | |
Accessioni | Q9EQH4Primary (citable) accession number: Q9EQH4 Secondary accession number(s): Q3TSE4 Q8C7L7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 15, 2008 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 141 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families