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UniProtKB - Q9EQG7 (ENPP5_MOUSE)
Protein
Ectonucleotide pyrophosphatase/phosphodiesterase family member 5
Gene
Enpp5
Organism
Mus musculus (Mouse)
Status
Functioni
Can hydrolyze NAD but cannot hydrolyze nucleotide di- and triphosphates (PubMed:28898552).
Lacks lysopholipase D activity. May play a role in neuronal cell communication (By similarity).
By similarity1 PublicationCofactori
Zn2+1 PublicationNote: Binds 2 Zn2+ ions per subunit.1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 36 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Active sitei | 72 | NucleophileSequence analysisCombined sources1 Publication | 1 | |
Metal bindingi | 72 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 191 | Zinc 2; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 195 | Zinc 2; via tele nitrogen; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 238 | Zinc 1; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 239 | Zinc 1; via tele nitrogen; catalyticCombined sources1 Publication | 1 | |
Metal bindingi | 339 | Zinc 2; via tele nitrogen; catalyticCombined sources1 Publication | 1 |
GO - Molecular functioni
- NAD+ diphosphatase activity Source: MGI
- nucleotide diphosphatase activity Source: MGI
- zinc ion binding Source: UniProtKB
GO - Biological processi
- cell communication Source: MGI
- nucleotide catabolic process Source: MGI
Keywordsi
Molecular function | Hydrolase |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
BRENDAi | 3.6.1.9, 3474 |
Names & Taxonomyi
Protein namesi | Recommended name: Ectonucleotide pyrophosphatase/phosphodiesterase family member 5 (EC:3.1.-.-)Short name: E-NPP 5 Short name: NPP-5 |
Gene namesi | Name:Enpp5Imported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1933830, Enpp5 |
VEuPathDBi | HostDB:ENSMUSG00000023960 |
Subcellular locationi
Extracellular region or secreted
- Secreted Curated
Other locations
- Membrane Curated; Single-pass membrane protein Curated
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Plasma Membrane
- integral component of plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- membrane Source: MGI
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 432 – 452 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Membrane, SecretedPathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 72 | T → A: Catalytically inactive. 1 Publication | 1 | |
Mutagenesisi | 73 | Y → F: Can hydrolyze nucleotides, with about fourfold higher rates for adenine versus uridine and no strong preference for diphosphates or triphosphates. 1 Publication | 1 | |
Mutagenesisi | 159 | E → S: No effect on its ability to hydrolyze NAD. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 24 | By similarityAdd BLAST | 24 | |
ChainiPRO_0000036402 | 25 – 477 | Ectonucleotide pyrophosphatase/phosphodiesterase family member 5Add BLAST | 453 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 101 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 158 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 292 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 329 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 362 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 369 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 382 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 389 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
N-glycosylated.By similarity
Keywords - PTMi
GlycoproteinProteomic databases
MaxQBi | Q9EQG7 |
PaxDbi | Q9EQG7 |
PRIDEi | Q9EQG7 |
ProteomicsDBi | 277877 |
PTM databases
GlyConnecti | 2276, 6 N-Linked glycans (5 sites) |
GlyGeni | Q9EQG7, 9 sites, 6 N-linked glycans (5 sites) |
PhosphoSitePlusi | Q9EQG7 |
Expressioni
Tissue specificityi
Expressed abundantly in the brain and kidney, and at lower levels in the liver.1 Publication
Gene expression databases
Bgeei | ENSMUSG00000023960, Expressed in white adipose tissue and 256 other tissues |
ExpressionAtlasi | Q9EQG7, baseline and differential |
Genevisiblei | Q9EQG7, MM |
Interactioni
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000024756 |
Miscellaneous databases
RNActi | Q9EQG7, protein |
Structurei
Secondary structure
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details3D structure databases
SMRi | Q9EQG7 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Sequence similaritiesi
Belongs to the nucleotide pyrophosphatase/phosphodiesterase family.Curated
Keywords - Domaini
Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG2645, Eukaryota |
GeneTreei | ENSGT00940000160562 |
HOGENOMi | CLU_017594_1_2_1 |
InParanoidi | Q9EQG7 |
OMAi | MCHILSI |
OrthoDBi | 999163at2759 |
PhylomeDBi | Q9EQG7 |
TreeFami | TF330032 |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR002591, Phosphodiest/P_Trfase |
Pfami | View protein in Pfam PF01663, Phosphodiest, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9EQG7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIPEFLLASC TLATLCHSAP FSLQPEEQKV LVVSFDGFRW DYLYKVPTPH
60 70 80 90 100
FHYIMKNGVH VNQVTNVFIT KTYPNHYTLV TGLFAENHGI VANDMFDPIL
110 120 130 140 150
NKSFSLEHMD IYDSKFWEEA TPIWITNQRA GHASGAAMWP GADVKIHDSF
160 170 180 190 200
PTYYLPYNES VSFEDRVAKI IEWFTAKDPI NLGFLYWEEP DDTGHDVGPD
210 220 230 240 250
SPLMGSVISD VDHKLGYLIK MLKRAKLWNN VNLIVTSDHG MTQCSKQRVI
260 270 280 290 300
ELDRYLDKEH YTLIDHSPVA AILPKEGKFD EVYDALAGAH PNLTVYKKEE
310 320 330 340 350
IPERWHYKHN DRVQPIVAVA DEGWYILQNK SDDFLLGNHG YDNALAEMHP
360 370 380 390 400
IFLAHGPAFR KNFTKEAMNS TDLYSLLCHL LNLTALPHNG SFWNVQDLLS
410 420 430 440 450
SATPKPIPYT QSTTLLLGSD KPGEDEQEES YPYYIGVSLG SIIAMVFFVV
460 470
LIKHLIRSQV HTLQYRQVEV AQPLLQA
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketD6RH39 | D6RH39_MOUSE | Ectonucleotide pyrophosphatase/phos... | Enpp5 | 45 | Annotation score: |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233377 mRNA Translation: AAG49143.1 CT030230 Genomic DNA No translation available. BC138977 mRNA Translation: AAI38978.1 BC138978 mRNA Translation: AAI38979.1 |
CCDSi | CCDS28802.1 |
PIRi | A59390 |
RefSeqi | NP_001162091.1, NM_001168620.2 NP_001316548.1, NM_001329619.1 NP_114392.1, NM_032003.3 |
Genome annotation databases
Ensembli | ENSMUST00000024756; ENSMUSP00000024756; ENSMUSG00000023960 ENSMUST00000154166; ENSMUSP00000122767; ENSMUSG00000023960 |
GeneIDi | 83965 |
KEGGi | mmu:83965 |
UCSCi | uc008cpr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233377 mRNA Translation: AAG49143.1 CT030230 Genomic DNA No translation available. BC138977 mRNA Translation: AAI38978.1 BC138978 mRNA Translation: AAI38979.1 |
CCDSi | CCDS28802.1 |
PIRi | A59390 |
RefSeqi | NP_001162091.1, NM_001168620.2 NP_001316548.1, NM_001329619.1 NP_114392.1, NM_032003.3 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
5VEN | X-ray | 1.69 | A/B | 25-430 | [»] | |
5VEO | X-ray | 1.53 | A | 25-430 | [»] | |
SMRi | Q9EQG7 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
STRINGi | 10090.ENSMUSP00000024756 |
PTM databases
GlyConnecti | 2276, 6 N-Linked glycans (5 sites) |
GlyGeni | Q9EQG7, 9 sites, 6 N-linked glycans (5 sites) |
PhosphoSitePlusi | Q9EQG7 |
Proteomic databases
MaxQBi | Q9EQG7 |
PaxDbi | Q9EQG7 |
PRIDEi | Q9EQG7 |
ProteomicsDBi | 277877 |
Protocols and materials databases
Antibodypediai | 2350, 162 antibodies from 24 providers |
DNASUi | 83965 |
Genome annotation databases
Ensembli | ENSMUST00000024756; ENSMUSP00000024756; ENSMUSG00000023960 ENSMUST00000154166; ENSMUSP00000122767; ENSMUSG00000023960 |
GeneIDi | 83965 |
KEGGi | mmu:83965 |
UCSCi | uc008cpr.2, mouse |
Organism-specific databases
CTDi | 59084 |
MGIi | MGI:1933830, Enpp5 |
VEuPathDBi | HostDB:ENSMUSG00000023960 |
Phylogenomic databases
eggNOGi | KOG2645, Eukaryota |
GeneTreei | ENSGT00940000160562 |
HOGENOMi | CLU_017594_1_2_1 |
InParanoidi | Q9EQG7 |
OMAi | MCHILSI |
OrthoDBi | 999163at2759 |
PhylomeDBi | Q9EQG7 |
TreeFami | TF330032 |
Enzyme and pathway databases
BRENDAi | 3.6.1.9, 3474 |
Miscellaneous databases
BioGRID-ORCSi | 83965, 1 hit in 63 CRISPR screens |
ChiTaRSi | Enpp5, mouse |
PROi | PR:Q9EQG7 |
RNActi | Q9EQG7, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000023960, Expressed in white adipose tissue and 256 other tissues |
ExpressionAtlasi | Q9EQG7, baseline and differential |
Genevisiblei | Q9EQG7, MM |
Family and domain databases
Gene3Di | 3.40.720.10, 1 hit |
InterProi | View protein in InterPro IPR017850, Alkaline_phosphatase_core_sf IPR002591, Phosphodiest/P_Trfase |
Pfami | View protein in Pfam PF01663, Phosphodiest, 1 hit |
SUPFAMi | SSF53649, SSF53649, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ENPP5_MOUSE | |
Accessioni | Q9EQG7Primary (citable) accession number: Q9EQG7 Secondary accession number(s): A9C479, Q921P7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2004 |
Last sequence update: | November 24, 2009 | |
Last modified: | February 23, 2022 | |
This is version 139 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families