UniProtKB - Q9EQG6 (KDIS_RAT)
Kinase D-interacting substrate of 220 kDa
Kidins220
Functioni
GO - Molecular functioni
- PDZ domain binding Source: UniProtKB
- protein kinase binding Source: RGD
- protein kinase regulator activity Source: RGD
GO - Biological processi
- cellular response to nerve growth factor stimulus Source: UniProtKB
- dendrite morphogenesis Source: RGD
- in utero embryonic development Source: RGD
- nerve growth factor signaling pathway Source: UniProtKB
- positive regulation of neuron projection development Source: UniProtKB
Keywordsi
Biological process | Neurogenesis |
Enzyme and pathway databases
Reactomei | R-RNO-170984, ARMS-mediated activation |
Names & Taxonomyi
Protein namesi | Recommended name: Kinase D-interacting substrate of 220 kDaAlternative name(s): Ankyrin repeat-rich membrane-spanning protein |
Gene namesi | Name:Kidins220 Synonyms:Arms |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619949, Kidins220 |
Subcellular locationi
Endosome
- Late endosome 2 Publications
Other locations
- Membrane Curated; Multi-pass membrane protein Curated
Note: Localized at late endosome before or after nerve growth factor (NGF) stimulation.
Cytosol
- cytosol Source: Reactome
Endosome
- late endosome Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
- protein-containing complex Source: UniProtKB
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 508 | CytoplasmicCuratedAdd BLAST | 508 | |
Transmembranei | 509 – 529 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 530 – 533 | ExtracellularCurated | 4 | |
Transmembranei | 534 – 554 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 555 – 668 | CytoplasmicCuratedAdd BLAST | 114 | |
Transmembranei | 669 – 689 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 690 – 696 | ExtracellularCurated | 7 | |
Transmembranei | 697 – 717 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 718 – 1680 | CytoplasmicCuratedAdd BLAST | 963 |
Keywords - Cellular componenti
Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 918 | S → A: Loss of phosphorylation. 1 Publication | 1 | |
Mutagenesisi | 1760 | S → D: Loss of binding to PDZ domain of SNTA1 and SNTB2. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000322120 | 1 – 1762 | Kinase D-interacting substrate of 220 kDaAdd BLAST | 1762 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 882 | PhosphoserineBy similarity | 1 | |
Modified residuei | 885 | PhosphoserineBy similarity | 1 | |
Modified residuei | 914 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 918 | Phosphoserine; by PKDCombined sources1 Publication | 1 | |
Modified residuei | 1163 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1288 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1344 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1351 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1353 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1354 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1357 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1513 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1518 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1547 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1566 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1615 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1625 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1671 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 1673 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1676 | PhosphothreonineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
jPOSTi | Q9EQG6 |
PaxDbi | Q9EQG6 |
PRIDEi | Q9EQG6 |
PTM databases
iPTMneti | Q9EQG6 |
PhosphoSitePlusi | Q9EQG6 |
SwissPalmi | Q9EQG6 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Found in a complex, at least composed of KIDINS220, MAGI2, NTRK1 and RAPGEF2; the complex is mainly formed at late endosomes in a nerve growth factor (NGF)-dependent manner (PubMed:17724123).
Interacts with RAPGEF2; the interaction is strengthened after NGF stimulation (PubMed:17724123). Isoform 2 interacts (via C-terminal domain) with MAGI2 isoform 1 (via PDZ domain) (PubMed:17724123).
Interacts with NTRK1, NTRK2, NTRK3, ERKL and NGFR (PubMed:11150334, PubMed:15167895, PubMed:15378608). Can form a ternary complex with NGFR and NTRK1 and this complex is affected by the expression levels of KIDINS220/ARMS (PubMed:15378608). An increase in KIDINS220/ARMS expression leads to a decreased association of NGFR and NTRK1 (PubMed:15378608).
Interacts (via PDZ-binding motif) with SNTA1 and SNTB2 (via PDZ domains) (PubMed:15939763).
Interacts with EPHA4 and PRKD1 (PubMed:10998417, PubMed:15939763).
6 PublicationsBinary interactionsi
Q9EQG6
With | #Exp. | IntAct |
---|---|---|
CRKL [P46109] from Homo sapiens. | 2 | EBI-976654,EBI-910 |
GO - Molecular functioni
- PDZ domain binding Source: UniProtKB
- protein kinase binding Source: RGD
Protein-protein interaction databases
CORUMi | Q9EQG6 |
IntActi | Q9EQG6, 5 interactors |
STRINGi | 10116.ENSRNOP00000063889 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Repeati | 45 – 74 | ANK 1Sequence analysisAdd BLAST | 30 | |
Repeati | 78 – 107 | ANK 2Sequence analysisAdd BLAST | 30 | |
Repeati | 111 – 140 | ANK 3Sequence analysisAdd BLAST | 30 | |
Repeati | 145 – 174 | ANK 4Sequence analysisAdd BLAST | 30 | |
Repeati | 178 – 207 | ANK 5Sequence analysisAdd BLAST | 30 | |
Repeati | 211 – 240 | ANK 6Sequence analysisAdd BLAST | 30 | |
Repeati | 244 – 273 | ANK 7Sequence analysisAdd BLAST | 30 | |
Repeati | 277 – 306 | ANK 8Sequence analysisAdd BLAST | 30 | |
Repeati | 310 – 339 | ANK 9Sequence analysisAdd BLAST | 30 | |
Repeati | 343 – 372 | ANK 10Sequence analysisAdd BLAST | 30 | |
Repeati | 376 – 405 | ANK 11Sequence analysisAdd BLAST | 30 | |
Domaini | 440 – 953 | KAP NTPaseAdd BLAST | 514 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1089 – 1092 | Mediates interaction with CRKL1 Publication | 4 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1757 – 1762 | PDZ-binding1 Publication | 6 |
Domaini
Keywords - Domaini
ANK repeat, Repeat, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0502, Eukaryota |
InParanoidi | Q9EQG6 |
OrthoDBi | 58543at2759 |
PhylomeDBi | Q9EQG6 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 1.25.40.20, 3 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR011646, KAP_P-loop IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 4 hits PF07693, KAP_NTPase, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 11 hits |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 10 hits |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVLISQSVI NYVEEENIPA LKALLEKCKD VDERNECGQT PLMLAAEQGN
60 70 80 90 100
VEIVKELLKN GANCNLEDLD NWTALISASK EGHIHIVEEL LKSGASLEHR
110 120 130 140 150
DMGGWTALMW ACYKGRTDVV ELLLSHGANP SVTGLYSVYP IIWAAGRGHA
160 170 180 190 200
DIVHLLLQNG AKVNCSDKYG TTPLVWAARK GHLECVKHLL AMGADVDQEG
210 220 230 240 250
ANSMTALIVA VKGGYTQSVK EILKRNPNVN LTDKDGNTAL MIASKEGHIE
260 270 280 290 300
IVQDLLDAGT YVNIPDRSGD TVLIGAVRGG HVEIVRALLQ KYADIDIRGQ
310 320 330 340 350
DNKTALYWAV EKGNATMVRD ILQCNPDTEI CTKDGETPLI KATKMRNIEV
360 370 380 390 400
VELLLDKGAK VSAVDKKGDT PLHVAIRGRS RRLAELLLRN PKDGRLLYRP
410 420 430 440 450
NKAGETPYNI DCSHQKSILT QIFGARHLSP TETDGDMLGY DLYSSALADI
460 470 480 490 500
LSEPTMQPPI CVGLYAQWGS GKSFLLKKLE DEMKTFAGQQ TEPLFQFSWL
510 520 530 540 550
IVFLTLLLCG GLGLVFAFTV DTNLAIAISL SFLALIYIFF IVIYFGGRRE
560 570 580 590 600
GESWNWAWAL STRLARHIGY LELLFKLMFV NPPELPEQTT KALPVRFLFT
610 620 630 640 650
DYNRLSSVGG ETSLAEMIAT LSDACEREFG FLATRLFRVF RTEESQGKKK
660 670 680 690 700
WKKTCCLPSF VIFLFIVGCI IAGITLLAIF RVDPKHLTVN AILISIASVV
710 720 730 740 750
GLAFVLNCRT WWQVLDSLLN SQRKRLHSAA SKLHKLKSEG FMKVLKCEVE
760 770 780 790 800
LMARMAKTID SFTQNQTRLV VIIDGLDACE QDKVLQMLDT VRVLFSKGPF
810 820 830 840 850
IAIFASDPHI IIKAINQNLN SVLRDSNING HDYMRNIVHL PVFLNSRGLS
860 870 880 890 900
NARKFLVTSA TNGDITCSDT TGTQEDTDRR VSQNSLGEMT KLGSKTALNR
910 920 930 940 950
RDTYRRRQMQ RTITRQMSFD LTKLLVTEDW FSDISPQTMR RLLNIVSVTG
960 970 980 990 1000
RLLRANQITF NWDRLASWIN LTEQWPYRTS WLILYLEETE GLPDQMTLKT
1010 1020 1030 1040 1050
IYERISKNIP TTKDVEPLLE IDGDIRNFEV FLSSRTPVLV ARDVKTFLPC
1060 1070 1080 1090 1100
TVNLDPKLRE IIADVRAARE QINIGGLAYP PLPLHEGPPR PPSGYSQPAS
1110 1120 1130 1140 1150
VCSSASFNGP FPGGVVSPQP HSSYYSGLSG PQHPFYNRPF FAPYLYTPRY
1160 1170 1180 1190 1200
YPGGSQHLIS RSSVKTSLPR DQNNGLPCDS GFNKQRQAAV PATGSSLLLS
1210 1220 1230 1240 1250
SMTVDVVCEK LRQIEGLDQS MMPQYCTTIK KANINGRVLS QCNIDELKKE
1260 1270 1280 1290 1300
MAMNFGDWHL FRSMVLEMRS VESQVVPEDP RFLNENSSAP VPHGESARRS
1310 1320 1330 1340 1350
SHTELPLTEL SSQTPYTLNF SFEELNTLGL DEGAPRHSNL SWQSQTRRTP
1360 1370 1380 1390 1400
SLSSLNSQDS SIEISKLTDK VQAEYRDAYR EYIAQMSQLE GGTGSSTISG
1410 1420 1430 1440 1450
RSSPHSTYYI GQSSSGGSIH STLEQERGKE GELKQEDGRK SFLMKRGDVI
1460 1470 1480 1490 1500
DYSSSGVSTN EASPLDPITE EDEKSDQSGS KLLPGKKSSE RPSLFQTDLK
1510 1520 1530 1540 1550
LKGGGLRYQK LPSDEDESGT EESDNTPLLK DDKDKKAEGK AERVCKSPEH
1560 1570 1580 1590 1600
SAEPIRTFIK AKEYLSDALL DKKDSSDSGV RSNESSPNHS LHNEAADDSQ
1610 1620 1630 1640 1650
LEKANLIELE DEGHSGKRGM PHSLSGLQDP IIARMSICSE DKKSPSECSL
1660 1670 1680 1690 1700
IASSPEESWP ACQKAYNLNR TPSTVTLNNN TAPTNRANQN FDEIEGIRET
1710 1720 1730 1740 1750
SQVILRPGPS PNPTAVQNEN LKSMAHKRSQ RSSYTRLSKD ASELHAASSE
1760
STGFGEERES IL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3ZWB2 | D3ZWB2_RAT | Kinase D-interacting substrate of 2... | Kidins220 | 1,762 | Annotation score: | ||
D4ABK9 | D4ABK9_RAT | Kinase D-interacting substrate of 2... | Kidins220 | 1,712 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 519 | T → P in AAG34167 (PubMed:11150334).Curated | 1 | |
Sequence conflicti | 1001 | I → M in AAG34167 (PubMed:11150334).Curated | 1 | |
Sequence conflicti | 1220 | S → N in AAG34167 (PubMed:11150334).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_031868 | 135 | L → LQ in isoform 2. 1 Publication | 1 | |
Alternative sequenceiVSP_031869 | 1139 – 1187 | Missing in isoform 2. 1 PublicationAdd BLAST | 49 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF239045 mRNA Translation: AAG35185.2 AF313464 mRNA Translation: AAG34167.1 Frameshift. |
RefSeqi | NP_446247.1, NM_053795.1 [Q9EQG6-1] |
Genome annotation databases
GeneIDi | 116478 |
KEGGi | rno:116478 |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF239045 mRNA Translation: AAG35185.2 AF313464 mRNA Translation: AAG34167.1 Frameshift. |
RefSeqi | NP_446247.1, NM_053795.1 [Q9EQG6-1] |
3D structure databases
SMRi | Q9EQG6 |
ModBasei | Search... |
Protein-protein interaction databases
CORUMi | Q9EQG6 |
IntActi | Q9EQG6, 5 interactors |
STRINGi | 10116.ENSRNOP00000063889 |
PTM databases
iPTMneti | Q9EQG6 |
PhosphoSitePlusi | Q9EQG6 |
SwissPalmi | Q9EQG6 |
Proteomic databases
jPOSTi | Q9EQG6 |
PaxDbi | Q9EQG6 |
PRIDEi | Q9EQG6 |
Genome annotation databases
GeneIDi | 116478 |
KEGGi | rno:116478 |
Organism-specific databases
CTDi | 57498 |
RGDi | 619949, Kidins220 |
Phylogenomic databases
eggNOGi | KOG0502, Eukaryota |
InParanoidi | Q9EQG6 |
OrthoDBi | 58543at2759 |
PhylomeDBi | Q9EQG6 |
Enzyme and pathway databases
Reactomei | R-RNO-170984, ARMS-mediated activation |
Miscellaneous databases
PROi | PR:Q9EQG6 |
Family and domain databases
Gene3Di | 1.10.150.50, 1 hit 1.25.40.20, 3 hits |
InterProi | View protein in InterPro IPR002110, Ankyrin_rpt IPR020683, Ankyrin_rpt-contain_dom IPR036770, Ankyrin_rpt-contain_sf IPR011646, KAP_P-loop IPR013761, SAM/pointed_sf |
Pfami | View protein in Pfam PF12796, Ank_2, 4 hits PF07693, KAP_NTPase, 1 hit |
PRINTSi | PR01415, ANKYRIN |
SMARTi | View protein in SMART SM00248, ANK, 11 hits |
SUPFAMi | SSF47769, SSF47769, 1 hit SSF48403, SSF48403, 1 hit |
PROSITEi | View protein in PROSITE PS50297, ANK_REP_REGION, 1 hit PS50088, ANK_REPEAT, 10 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | KDIS_RAT | |
Accessioni | Q9EQG6Primary (citable) accession number: Q9EQG6 Secondary accession number(s): Q9ERD4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | February 26, 2008 |
Last sequence update: | October 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 121 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |