Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Dihydropyrimidinase

Gene

Dpys

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the second step of the reductive pyrimidine degradation, the reversible hydrolytic ring opening of dihydropyrimidines. Can catalyze the ring opening of 5,6-dihydrouracil to N-carbamyl-alanine and of 5,6-dihydrothymine to N-carbamyl-amino isobutyrate (By similarity).By similarity

Catalytic activityi

5,6-dihydrouracil + H2O = 3-ureidopropanoate.By similarity

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi67Zinc 1By similarity1
Metal bindingi69Zinc 1By similarity1
Metal bindingi159Zinc 1; via carbamate groupBy similarity1
Metal bindingi159Zinc 2; via carbamate groupBy similarity1
Binding sitei164SubstrateBy similarity1
Metal bindingi192Zinc 2By similarity1
Metal bindingi248Zinc 2By similarity1
Metal bindingi326Zinc 1By similarity1
Binding sitei347Substrate; via carbonyl oxygenBy similarity1

GO - Molecular functioni

  • amino acid binding Source: BHF-UCL
  • dihydropyrimidinase activity Source: BHF-UCL
  • phosphoprotein binding Source: BHF-UCL
  • thymine binding Source: BHF-UCL
  • uracil binding Source: BHF-UCL
  • zinc ion binding Source: BHF-UCL

GO - Biological processi

  • beta-alanine metabolic process Source: BHF-UCL
  • protein homooligomerization Source: BHF-UCL
  • protein homotetramerization Source: BHF-UCL
  • pyrimidine nucleobase catabolic process Source: MGI
  • thymine catabolic process Source: BHF-UCL
  • uracil catabolic process Source: BHF-UCL
  • uracil metabolic process Source: MGI

Keywordsi

Molecular functionHydrolase
LigandMetal-binding, Zinc

Enzyme and pathway databases

BRENDAi3.5.2.2 3474
ReactomeiR-MMU-73621 Pyrimidine catabolism

Protein family/group databases

MEROPSiM38.973

Names & Taxonomyi

Protein namesi
Recommended name:
Dihydropyrimidinase (EC:3.5.2.2By similarity)
Short name:
DHP
Short name:
DHPase
Alternative name(s):
Dihydropyrimidine amidohydrolase
Hydantoinase
Gene namesi
Name:Dpys
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1928679 Dpys

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001659071 – 519DihydropyrimidinaseAdd BLAST519

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei79PhosphoserineBy similarity1
Modified residuei159N6-carboxylysineBy similarity1
Modified residuei256N6-succinyllysineCombined sources1
Modified residuei510PhosphothreonineBy similarity1

Post-translational modificationi

Carbamylation allows a single lysine to coordinate two zinc ions.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiQ9EQF5
PaxDbiQ9EQF5
PeptideAtlasiQ9EQF5
PRIDEiQ9EQF5

PTM databases

iPTMnetiQ9EQF5
PhosphoSitePlusiQ9EQF5
SwissPalmiQ9EQF5

Expressioni

Gene expression databases

BgeeiENSMUSG00000022304
CleanExiMM_DPYS
ExpressionAtlasiQ9EQF5 baseline and differential
GenevisibleiQ9EQF5 MM

Interactioni

Subunit structurei

Homotetramer.By similarity

GO - Molecular functioni

  • phosphoprotein binding Source: BHF-UCL

Protein-protein interaction databases

BioGridi211101, 1 interactor
IntActiQ9EQF5, 1 interactor
MINTiQ9EQF5
STRINGi10090.ENSMUSP00000022915

Structurei

3D structure databases

ProteinModelPortaliQ9EQF5
SMRiQ9EQF5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG2584 Eukaryota
COG0044 LUCA
GeneTreeiENSGT00760000119241
HOGENOMiHOG000219145
HOVERGENiHBG000806
InParanoidiQ9EQF5
KOiK01464
OMAiIDPQVHF
OrthoDBiEOG091G05F3
PhylomeDBiQ9EQF5
TreeFamiTF314706

Family and domain databases

CDDicd01314 D-HYD, 1 hit
Gene3Di2.30.40.10, 2 hits
InterProiView protein in InterPro
IPR006680 Amidohydro-rel
IPR011778 Hydantoinase/dihydroPyrase
IPR011059 Metal-dep_hydrolase_composite
IPR032466 Metal_Hydrolase
PfamiView protein in Pfam
PF01979 Amidohydro_1, 1 hit
SUPFAMiSSF51338 SSF51338, 2 hits
SSF51556 SSF51556, 1 hit
TIGRFAMsiTIGR02033 D-hydantoinase, 1 hit

Sequencei

Sequence statusi: Complete.

Q9EQF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAPQGRLLIR GGRIVNDDFS QVADVLVEDG VVRALGRDLL PPEDASRGLR
60 70 80 90 100
ILDAAGKLVL PGGIDTHTHM QFPFMGSQSV DDFYQGTKAA LAGGTTMIID
110 120 130 140 150
FAIPQKGSSL IEAFETWRNW ADPKVCCDYS LHVAVTWWSD KVKEEMKTLA
160 170 180 190 200
RDKGVNSFKM FMAYKGLYMV QDEQLYAAFS QCKEIGAIAQ VHAENGDLIA
210 220 230 240 250
EGAKKMLALG ITGPEGHELC RPEAVEAEAT LRAITIASAV NCPLYVVHVM
260 270 280 290 300
SKSAAKVVAD ARRAGNVVYG EPIAAGLGTD GRQYWSEEWS HAAHHVMGPP
310 320 330 340 350
LRPDPLTPGF LMDLLANGDL TTTGSDNCTF NTCQKALGKD DFTKIPNGVN
360 370 380 390 400
GVEDRMSVIW EKGVHSGKMD ENRFVAVTST NAAKIFNLYP KKGRIAVGSD
410 420 430 440 450
ADIVIWDPEA TRRISAKTHH QAVNFNIFEG MVCHGVPLVT ISRGRVVYEA
460 470 480 490 500
GVFNVTAGHG KFIPRQPFAE YIYKRIKQRD QTCTPVPVKR APYKGEVTTL
510
KARETKEDDT AGTRMQGHS
Length:519
Mass (Da):56,725
Last modified:June 6, 2002 - v2
Checksum:iF2C53D40EC120ABD
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti13R → L in AAG37999 (PubMed:10956643).Curated1
Sequence conflicti13R → L in AAH37086 (PubMed:15489334).Curated1
Sequence conflicti37R → P in AAK00644 (Ref. 2) Curated1
Sequence conflicti43E → G in AAK00644 (Ref. 2) Curated1
Sequence conflicti206M → T in BAB23654 (PubMed:16141072).Curated1
Sequence conflicti299P → L in AAK00644 (Ref. 2) Curated1
Sequence conflicti366S → N in AAK00644 (Ref. 2) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF249296 mRNA Translation: AAG37999.1
AF227731 mRNA Translation: AAK00644.1
AK004822 mRNA Translation: BAB23593.1
AK004899 mRNA Translation: BAB23654.1
BC037086 mRNA Translation: AAH37086.1
CCDSiCCDS27445.1
RefSeqiNP_001157938.1, NM_001164466.1
NP_073559.3, NM_022722.3
UniGeneiMm.275974

Genome annotation databases

EnsembliENSMUST00000022915; ENSMUSP00000022915; ENSMUSG00000022304
ENSMUST00000110306; ENSMUSP00000105935; ENSMUSG00000022304
GeneIDi64705
KEGGimmu:64705
UCSCiuc007vok.2 mouse

Similar proteinsi

Entry informationi

Entry nameiDPYS_MOUSE
AccessioniPrimary (citable) accession number: Q9EQF5
Secondary accession number(s): Q99PP1, Q9DBK3, Q9DBP7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 6, 2002
Last sequence update: June 6, 2002
Last modified: June 20, 2018
This is version 136 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

We'd like to inform you that we have updated our Privacy Notice to comply with Europe’s new General Data Protection Regulation (GDPR) that applies since 25 May 2018.

Do not show this banner again
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health