UniProtKB - Q9EQ76 (FMO3_RAT)
Dimethylaniline monooxygenase [N-oxide-forming] 3
Fmo3
Functioni
Essential hepatic enzyme that catalyzes the oxygenation of a wide variety of nitrogen- and sulfur-containing compounds including drugs as well as dietary compounds. Plays an important role in the metabolism of trimethylamine (TMA), via the production of trimethylamine N-oxide (TMAO) metabolite. TMA is generated by the action of gut microbiota using dietary precursors such as choline, choline containing compounds, betaine or L-carnitine. By regulating TMAO concentration, FMO3 directly impacts both platelet responsiveness and rate of thrombus formation.
By similarity2 PublicationsCatalytic activityi
- EC:1.14.13.8By similarity
- EC:1.14.13.148By similarity
- fenbendazole + O2 + reduced [NADPH—hemoprotein reductase] = fenbendazole S-oxide + H+ + H2O + oxidized [NADPH—hemoprotein reductase]By similarityEC:1.14.14.73By similarity
- albendazole + O2 + reduced [NADPH—hemoprotein reductase] = albendazole S-oxide + H+ + H2O + oxidized [NADPH—hemoprotein reductase]2 PublicationsEC:1.14.14.732 Publications
Cofactori
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 32 | FADBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 9 – 13 | FADBy similarity | 5 | |
Nucleotide bindingi | 40 – 41 | FADBy similarity | 2 | |
Nucleotide bindingi | 60 – 61 | NADPBy similarity | 2 | |
Nucleotide bindingi | 61 – 62 | FADBy similarity | 2 | |
Nucleotide bindingi | 195 – 198 | NADPBy similarity | 4 |
GO - Molecular functioni
- amino acid binding Source: RGD
- flavin adenine dinucleotide binding Source: InterPro
- monooxygenase activity Source: GO_Central
- N,N-dimethylaniline monooxygenase activity Source: RGD
- NADP binding Source: RGD
- trimethylamine monooxygenase activity Source: UniProtKB-EC
GO - Biological processi
- xenobiotic metabolic process Source: RGD
Keywordsi
Molecular function | Monooxygenase, Oxidoreductase |
Ligand | FAD, Flavoprotein, NADP |
Enzyme and pathway databases
BRENDAi | 1.14.13.8, 5301 |
Reactomei | R-RNO-217271, FMO oxidises nucleophiles |
SABIO-RKi | Q9EQ76 |
Names & Taxonomyi
Protein namesi | Recommended name: Dimethylaniline monooxygenase [N-oxide-forming] 3 (EC:1.14.13.8By similarity, EC:1.14.14.73By similarity)Alternative name(s): Dimethylaniline oxidase 3 Hepatic flavin-containing monooxygenase 3 Short name: FMO 3 Trimethylamine monooxygenase (EC:1.14.13.148) |
Gene namesi | Name:Fmo3 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 619761, Fmo3 |
Subcellular locationi
Endoplasmic reticulum
- Microsome membrane 1 Publication; Single-pass membrane protein Sequence analysis
- Endoplasmic reticulum membrane By similarity; Single-pass membrane protein Sequence analysis
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
- intracellular membrane-bounded organelle Source: RGD
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Transmembranei | 511 – 531 | HelicalSequence analysisAdd BLAST | 21 |
Keywords - Cellular componenti
Endoplasmic reticulum, Membrane, MicrosomePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000147659 | 1 – 531 | Dimethylaniline monooxygenase [N-oxide-forming] 3Add BLAST | 531 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 401 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
PaxDbi | Q9EQ76 |
PRIDEi | Q9EQ76 |
PTM databases
CarbonylDBi | Q9EQ76 |
iPTMneti | Q9EQ76 |
PhosphoSitePlusi | Q9EQ76 |
Expressioni
Tissue specificityi
Interactioni
Protein-protein interaction databases
IntActi | Q9EQ76, 1 interactor |
STRINGi | 10116.ENSRNOP00000004864 |
Family & Domainsi
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG1399, Eukaryota |
InParanoidi | Q9EQ76 |
OrthoDBi | 405736at2759 |
PhylomeDBi | Q9EQ76 |
TreeFami | TF105285 |
Family and domain databases
Gene3Di | 3.50.50.60, 3 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR000960, Flavin_mOase IPR020946, Flavin_mOase-like IPR002255, Flavin_mOase_3 |
Pfami | View protein in Pfam PF00743, FMO-like, 1 hit |
PIRSFi | PIRSF000332, FMO, 1 hit |
PRINTSi | PR00370, FMOXYGENASE PR01123, FMOXYGENASE3 |
SUPFAMi | SSF51905, SSF51905, 2 hits |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MKRKVAVIGA GVSGLAAIRS CLEEGLEPTC FERSDDVGGL WKFSDHTEEG
60 70 80 90 100
RASIYQSVFT NSSKEMMCFP DFPYPDDFPN FMHNSKLQEY ITSFATEKNL
110 120 130 140 150
LKYIQFETLV TRINKCPDFS TTGKWEVTTE KNSKKETAVF DAVMICSGHH
160 170 180 190 200
VYPHLPKDSF PGLNRFKGKC FHSRDYKEPG TWKGKRVLVI GLGNSGCDIA
210 220 230 240 250
AELSHVAQQV IISSRSGSWV MSRVWNDGYP WDMVVITRFQ TFLKNNLPTA
260 270 280 290 300
ISDWWYMKQM NARFKHENYG LMPLNGTLRK EPVFNDELPA RILCGTVSIK
310 320 330 340 350
PNVKEFTETS AVFEDGTVFE GIDCVIFATG YGYAYPFLDD SIIKSRNNEV
360 370 380 390 400
TLYKGIFPPQ LEKPTMAVIG LVQSLGAAIP TTDLQARWAA QVIRGTCILP
410 420 430 440 450
SVNDMMDDID EKMGKKLKWF GNSTTIQTDY IVYMDELASF IGAKPNILWL
460 470 480 490 500
FLKDPRLAIE VFFGPCSPYQ FRLVGPGKWS GARNAILTQW DRSLKPMKTR
510 520 530
VVGGIQKPCL YSHFLRLLAV PVLIALFLVL I
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0G2JSI0 | A0A0G2JSI0_RAT | Dimethylaniline monooxygenase [N-ox... | Fmo3 rCG_46192 | 531 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 501 | V → D in AAH87008 (PubMed:15489334).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286595 mRNA Translation: AAG44891.1 BC087008 mRNA Translation: AAH87008.1 |
RefSeqi | NP_445885.2, NM_053433.2 |
Genome annotation databases
GeneIDi | 84493 |
KEGGi | rno:84493 |
UCSCi | RGD:619761, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF286595 mRNA Translation: AAG44891.1 BC087008 mRNA Translation: AAH87008.1 |
RefSeqi | NP_445885.2, NM_053433.2 |
3D structure databases
AlphaFoldDBi | Q9EQ76 |
SMRi | Q9EQ76 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9EQ76, 1 interactor |
STRINGi | 10116.ENSRNOP00000004864 |
PTM databases
CarbonylDBi | Q9EQ76 |
iPTMneti | Q9EQ76 |
PhosphoSitePlusi | Q9EQ76 |
Proteomic databases
PaxDbi | Q9EQ76 |
PRIDEi | Q9EQ76 |
Genome annotation databases
GeneIDi | 84493 |
KEGGi | rno:84493 |
UCSCi | RGD:619761, rat |
Organism-specific databases
CTDi | 2328 |
RGDi | 619761, Fmo3 |
Phylogenomic databases
eggNOGi | KOG1399, Eukaryota |
InParanoidi | Q9EQ76 |
OrthoDBi | 405736at2759 |
PhylomeDBi | Q9EQ76 |
TreeFami | TF105285 |
Enzyme and pathway databases
BRENDAi | 1.14.13.8, 5301 |
Reactomei | R-RNO-217271, FMO oxidises nucleophiles |
SABIO-RKi | Q9EQ76 |
Miscellaneous databases
PROi | PR:Q9EQ76 |
Family and domain databases
Gene3Di | 3.50.50.60, 3 hits |
InterProi | View protein in InterPro IPR036188, FAD/NAD-bd_sf IPR000960, Flavin_mOase IPR020946, Flavin_mOase-like IPR002255, Flavin_mOase_3 |
Pfami | View protein in Pfam PF00743, FMO-like, 1 hit |
PIRSFi | PIRSF000332, FMO, 1 hit |
PRINTSi | PR00370, FMOXYGENASE PR01123, FMOXYGENASE3 |
SUPFAMi | SSF51905, SSF51905, 2 hits |
MobiDBi | Search... |
Entry informationi
Entry namei | FMO3_RAT | |
Accessioni | Q9EQ76Primary (citable) accession number: Q9EQ76 Secondary accession number(s): Q5PQV6 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 25, 2005 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 131 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families