UniProtKB - Q9EPU2 (NCOA3_RAT)
Nuclear receptor coactivator 3
Ncoa3
Functioni
Nuclear receptor coactivator that directly binds nuclear receptors and stimulates the transcriptional activities in a hormone-dependent fashion. Plays a central role in creating a multisubunit coactivator complex, probably via remodeling of chromatin. Involved in the coactivation of different nuclear receptors, such as for steroids (GR and ER), retinoids (RARs and RXRs), thyroid hormone (TRs), vitamin D3 (VDR) and prostanoids (PPARs). Displays histone acetyltransferase activity. Also involved in the coactivation of the NF-kappa-B pathway via its interaction with the NFKB1 subunit (By similarity).
By similarityCatalytic activityi
- EC:2.3.1.48
Activity regulationi
GO - Molecular functioni
- androgen receptor binding Source: RGD
- chromatin binding Source: RGD
- disordered domain specific binding Source: RGD
- DNA-binding transcription activator activity, RNA polymerase II-specific Source: RGD
- estrogen receptor binding Source: RGD
- histone acetyltransferase activity Source: RGD
- nuclear receptor binding Source: RGD
- nuclear receptor coactivator activity Source: RGD
- peroxisome proliferator activated receptor binding Source: RGD
- protein-containing complex binding Source: RGD
- protein N-terminus binding Source: RGD
- RNA polymerase II complex binding Source: UniProtKB
- RNA polymerase II transcription regulatory region sequence-specific DNA binding Source: RGD
- transcription coactivator activity Source: RGD
GO - Biological processi
- cell dedifferentiation Source: UniProtKB
- cellular response to estradiol stimulus Source: RGD
- cellular response to hormone stimulus Source: GO_Central
- developmental growth Source: RGD
- labyrinthine layer morphogenesis Source: RGD
- male gonad development Source: RGD
- mammary gland branching involved in thelarche Source: RGD
- multicellular organism growth Source: RGD
- negative regulation of apoptotic process Source: RGD
- negative regulation of stem cell differentiation Source: RGD
- positive regulation of cell growth Source: RGD
- positive regulation of DNA-binding transcription factor activity Source: RGD
- positive regulation of gene expression Source: RGD
- positive regulation of intracellular estrogen receptor signaling pathway Source: RGD
- positive regulation of intracellular steroid hormone receptor signaling pathway Source: RGD
- positive regulation of keratinocyte differentiation Source: RGD
- positive regulation of stem cell population maintenance Source: UniProtKB
- positive regulation of transcription by RNA polymerase II Source: RGD
- regulation of stem cell division Source: UniProtKB
- regulation of stem cell population maintenance Source: RGD
- regulation of stem cell proliferation Source: RGD
- vagina development Source: RGD
Keywordsi
Molecular function | Activator, Acyltransferase, Transferase |
Biological process | Transcription, Transcription regulation |
Enzyme and pathway databases
Reactomei | R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-9018519, Estrogen-dependent gene expression |
Names & Taxonomyi
Protein namesi | Recommended name: Nuclear receptor coactivator 3 (EC:2.3.1.48)Short name: NCoA-3 Alternative name(s): Amplified in breast cancer-1 protein homolog Short name: AIB-1 |
Gene namesi | Name:Ncoa3 Synonyms:Aib1 |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 620109, Ncoa3 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus Source: RGD
Nucleus
- nucleus Source: RGD
- RNA polymerase II transcription regulator complex Source: RGD
Other locations
- chromatin Source: RGD
- cytoplasm Source: RGD
- perinuclear region of cytoplasm Source: RGD
- protein-containing complex Source: RGD
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000094408 | ‹1 – 1082 | Nuclear receptor coactivator 3Add BLAST | ›1082 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 251 | PhosphoserineBy similarity | 1 | |
Modified residuei | 269 | PhosphoserineBy similarity | 1 | |
Modified residuei | 316 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 319 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 320 | N6-acetyllysine; by CREBBPBy similarity | 1 | |
Modified residuei | 387 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 394 | PhosphoserineBy similarity | 1 | |
Modified residuei | 427 | PhosphoserineBy similarity | 1 | |
Modified residuei | 551 | PhosphoserineBy similarity | 1 | |
Modified residuei | 554 | PhosphoserineBy similarity | 1 | |
Modified residuei | 722 | PhosphoserineBy similarity | 1 | |
Modified residuei | 860 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 866 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 877 | Asymmetric dimethylarginineBy similarity | 1 | |
Modified residuei | 988 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
PaxDbi | Q9EPU2 |
PTM databases
iPTMneti | Q9EPU2 |
PhosphoSitePlusi | Q9EPU2 |
Expressioni
Tissue specificityi
Interactioni
Subunit structurei
Present in a complex containing NCOA2, IKKA, IKKB, IKBKG and the histone acetyltransferase protein CREBBP.
Interacts with PCAF and CARM1.
Interacts with CASP8AP2 and NR3C1 (By similarity).
Interacts with ATAD2 and this interaction is enhanced by estradiol (By similarity).
Interacts with PSMB9. Binds to CSNK1D (By similarity).
Found in a complex containing NCOA3, AR and MAK.
Interacts with DDX5.
Interacts with NPAS2 (By similarity).
Interacts with NR4A3 (By similarity).
Interacts with ESRRB; mediates the interaction between ESRRB and RNA polymerase II complexes and allows NCOA3 corecruitment to ESRRB, KLF4, NANOG, and SOX2 enhancer regions to trigger ESRRB-dependent gene activation involved in self-renewal and pluripotency (By similarity).
By similarityGO - Molecular functioni
- androgen receptor binding Source: RGD
- disordered domain specific binding Source: RGD
- estrogen receptor binding Source: RGD
- nuclear receptor binding Source: RGD
- peroxisome proliferator activated receptor binding Source: RGD
- protein N-terminus binding Source: RGD
- RNA polymerase II complex binding Source: UniProtKB
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059144 |
Structurei
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 79 – 104 | DisorderedSequence analysisAdd BLAST | 26 | |
Regioni | 138 – 219 | DisorderedSequence analysisAdd BLAST | 82 | |
Regioni | 236 – 379 | DisorderedSequence analysisAdd BLAST | 144 | |
Regioni | 399 – 430 | DisorderedSequence analysisAdd BLAST | 32 | |
Regioni | 447 – 487 | DisorderedSequence analysisAdd BLAST | 41 | |
Regioni | 503 – 547 | DisorderedSequence analysisAdd BLAST | 45 | |
Regioni | 581 – 663 | DisorderedSequence analysisAdd BLAST | 83 | |
Regioni | 686 – 739 | DisorderedSequence analysisAdd BLAST | 54 | |
Regioni | 712 – 782 | Interaction with CREBBPBy similarityAdd BLAST | 71 | |
Regioni | 786 – 962 | AcetyltransferaseAdd BLAST | 177 | |
Regioni | 933 – 1004 | DisorderedSequence analysisAdd BLAST | 72 | |
Regioni | 1016 – 1039 | DisorderedSequence analysisAdd BLAST | 24 | |
Regioni | 1059 – 1082 | DisorderedSequence analysisAdd BLAST | 24 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 385 – 389 | LXXLL motif 1 | 5 | |
Motifi | 437 – 441 | LXXLL motif 2 | 5 | |
Motifi | 723 – 727 | LXXLL motif 3 | 5 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 138 – 193 | Polar residuesSequence analysisAdd BLAST | 56 | |
Compositional biasi | 203 – 219 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 236 – 272 | Polar residuesSequence analysisAdd BLAST | 37 | |
Compositional biasi | 328 – 377 | Polar residuesSequence analysisAdd BLAST | 50 | |
Compositional biasi | 405 – 430 | Polar residuesSequence analysisAdd BLAST | 26 | |
Compositional biasi | 460 – 479 | Polar residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 504 – 520 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 528 – 544 | Polar residuesSequence analysisAdd BLAST | 17 | |
Compositional biasi | 593 – 617 | Polar residuesSequence analysisAdd BLAST | 25 | |
Compositional biasi | 989 – 1004 | Polar residuesSequence analysisAdd BLAST | 16 |
Domaini
Sequence similaritiesi
Keywords - Domaini
RepeatPhylogenomic databases
eggNOGi | KOG3561, Eukaryota |
InParanoidi | Q9EPU2 |
PhylomeDBi | Q9EPU2 |
Family and domain databases
Gene3Di | 6.10.140.410, 1 hit |
InterProi | View protein in InterPro IPR032565, DUF4927 IPR010011, NCO_DUF1518 IPR028818, NCOA3 IPR009110, Nuc_rcpt_coact IPR014920, Nuc_rcpt_coact_Ncoa-typ IPR037077, Nuc_rcpt_coact_Ncoa_int_sf IPR017426, Nuclear_rcpt_coactivator IPR035965, PAS-like_dom_sf IPR014935, SRC/p160_LXXLL |
PANTHERi | PTHR10684, PTHR10684, 1 hit PTHR10684:SF3, PTHR10684:SF3, 1 hit |
Pfami | View protein in Pfam PF07469, DUF1518, 1 hit PF16279, DUF4927, 1 hit PF08815, Nuc_rec_co-act, 1 hit PF08832, SRC-1, 1 hit |
SMARTi | View protein in SMART SM01151, DUF1518, 1 hit |
SUPFAMi | SSF55785, SSF55785, 1 hit SSF69125, SSF69125, 1 hit |
(1+)i Sequence
Sequence statusi: Fragment.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
IIRRCIQRFF SLNDGQSWSQ KRHYQEAYIH GHAETPVYRF SLADGTIVSA
60 70 80 90 100
QTKSKLFRNP VTNDRHGFVS THFLQREQNG CRPNPILQDK GIRPPAAGCG
110 120 130 140 150
MSLSPSQSVQ MLGSRTYGVA DPSNTGQMAG ARYGASSSVA SLTPGQSLQS
160 170 180 190 200
PSSYQSNSYG LNMSSPPHGS PGLGPNQQNI MISPRNRGSP KMASHQFSPA
210 220 230 240 250
AGVHSPMGSS GNTGSHSFSS SSLSALQAIS EGVGTSLLST LSSPGPKLDN
260 270 280 290 300
SPNMNINQPS KASSQDSKSP LGLYCEQNPV ESSVCPSNSR DPPVTKENKE
310 320 330 340 350
NSGEASETPR GPLESKGHKK LLQLLTCSSD DRGHSSLTNS PLDSNCKDSS
360 370 380 390 400
ISVTSPSGVS SSTSGAVSST SNMHGSLLQE KHRILHKLLQ NGNSPAEVAK
410 420 430 440 450
ITAEATGKDT SSTASGGEGS VXQEQLSPXK KENNALLRYL LDRDDPSDVL
460 470 480 490 500
AKELQPQADG GDSKLSQCSC XTNPSSGQEK DPKIKTEEVS GDLDNLDAIL
510 520 530 540 550
GDLTSSDFYN SPTNGSHPGA KQQMFAGPSS LGLRSPQPVQ SVRPPYNRAL
560 570 580 590 600
SLDSPVSVGS VPPVKNVSAF PVLPKQPILA GNPRMMDSQE NYGANMGGPN
610 620 630 640 650
RNVPVNPTSS SGDWGLANSR ASRMEPLASS PLGRAGGDYS AALPRPALGS
660 670 680 690 700
SGPTLPLRSN RLPGARPTLM LQMRAGEVPM GMGVSPYSPA VPSNQPGSWP
710 720 730 740 750
EGMLSMEQGP HGAQNRPLLR NSLDELLGPP SNPEGQSDER ALLDQLHTLL
760 770 780 790 800
SNTDATGLEE IDRALGIPEL VSQGQALESK QDVFQGQEAA VMMDQKAALY
810 820 830 840 850
GQTYPAQGPP LQGGFHLQGQ SPSLNSMMSQ ISQQGSFPLQ GLHPRASMVR
860 870 880 890 900
PRTNTPKQLR MQLQQRLQGQ QFLNQSRQAL EMKMESPTGA AVMRPMLQSQ
910 920 930 940 950
QAFFNAQMAA QQKRELMNHH LQQQRMAMMM SQPQPQAFSP PPNVTASPSM
960 970 980 990 1000
DGVLAGSAMP QAPPQQFPYA TNYGMGQPPE PAFGRGSSPP SAMMSSRMGP
1010 1020 1030 1040 1050
SQNAMVQHPQ TAPMYQSSEM KGWPSGNLAR NGSFPQQQFA PQANPAAYNM
1060 1070 1080
VHMNSSGSHL GQMTMTPMPM SGMPMGPDQK YC
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF1M8E5 | F1M8E5_RAT | Nuclear receptor coactivator | Ncoa3 | 1,400 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Non-terminal residuei | 1 | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF322224 mRNA Translation: AAG42837.1 |
Genome annotation databases
UCSCi | RGD:620109, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF322224 mRNA Translation: AAG42837.1 |
3D structure databases
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 10116.ENSRNOP00000059144 |
PTM databases
iPTMneti | Q9EPU2 |
PhosphoSitePlusi | Q9EPU2 |
Proteomic databases
PaxDbi | Q9EPU2 |
Genome annotation databases
UCSCi | RGD:620109, rat |
Organism-specific databases
RGDi | 620109, Ncoa3 |
Phylogenomic databases
eggNOGi | KOG3561, Eukaryota |
InParanoidi | Q9EPU2 |
PhylomeDBi | Q9EPU2 |
Enzyme and pathway databases
Reactomei | R-RNO-5687128, MAPK6/MAPK4 signaling R-RNO-9018519, Estrogen-dependent gene expression |
Family and domain databases
Gene3Di | 6.10.140.410, 1 hit |
InterProi | View protein in InterPro IPR032565, DUF4927 IPR010011, NCO_DUF1518 IPR028818, NCOA3 IPR009110, Nuc_rcpt_coact IPR014920, Nuc_rcpt_coact_Ncoa-typ IPR037077, Nuc_rcpt_coact_Ncoa_int_sf IPR017426, Nuclear_rcpt_coactivator IPR035965, PAS-like_dom_sf IPR014935, SRC/p160_LXXLL |
PANTHERi | PTHR10684, PTHR10684, 1 hit PTHR10684:SF3, PTHR10684:SF3, 1 hit |
Pfami | View protein in Pfam PF07469, DUF1518, 1 hit PF16279, DUF4927, 1 hit PF08815, Nuc_rec_co-act, 1 hit PF08832, SRC-1, 1 hit |
SMARTi | View protein in SMART SM01151, DUF1518, 1 hit |
SUPFAMi | SSF55785, SSF55785, 1 hit SSF69125, SSF69125, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | NCOA3_RAT | |
Accessioni | Q9EPU2Primary (citable) accession number: Q9EPU2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | September 19, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 140 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families