UniProtKB - Q9EPU0 (RENT1_MOUSE)
Regulator of nonsense transcripts 1
Upf1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 481 | ATPBy similarity | 1 | |
Binding sitei | 671 | ATPBy similarity | 1 | |
Binding sitei | 708 | ATPBy similarity | 1 | |
Binding sitei | 839 | ATPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 116 – 267 | UPF1-typeAdd BLAST | 152 | |
Nucleotide bindingi | 501 – 505 | ATPBy similarity | 5 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- chromatin binding Source: HGNC-UCL
- RNA binding Source: UniProtKB
- RNA helicase activity Source: UniProtKB
- telomeric DNA binding Source: MGI
- zinc ion binding Source: InterPro
GO - Biological processi
- 3'-UTR-mediated mRNA destabilization Source: UniProtKB
- cell cycle phase transition Source: UniProtKB
- cellular response to interleukin-1 Source: UniProtKB
- cellular response to lipopolysaccharide Source: UniProtKB
- DNA repair Source: HGNC-UCL
- DNA replication Source: HGNC-UCL
- dosage compensation by inactivation of X chromosome Source: MGI
- histone mRNA catabolic process Source: UniProtKB
- nuclear-transcribed mRNA catabolic process Source: MGI
- nuclear-transcribed mRNA catabolic process, endonucleolytic cleavage-dependent decay Source: UniProtKB
- nuclear-transcribed mRNA catabolic process, nonsense-mediated decay Source: MGI
- positive regulation of mRNA catabolic process Source: UniProtKB
- regulation of telomere maintenance Source: MGI
- regulation of translational termination Source: HGNC-UCL
- telomere maintenance via semi-conservative replication Source: MGI
Keywordsi
Molecular function | Helicase, Hydrolase, RNA-binding |
Biological process | Nonsense-mediated mRNA decay |
Ligand | ATP-binding, Metal-binding, Nucleotide-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Names & Taxonomyi
Protein namesi | Recommended name: Regulator of nonsense transcripts 1 (EC:3.6.4.-)Alternative name(s): ATP-dependent helicase RENT1 Nonsense mRNA reducing factor 1 Short name: NORF1 Up-frameshift suppressor 1 homolog Short name: mUpf1 |
Gene namesi | Name:Upf1 Synonyms:Rent1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:107995, Upf1 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Other locations
- Cytoplasm 1 Publication
- P-body By similarity
- perinuclear region 1 Publication
Note: Hyperphosphorylated form is targeted to the P-body, while unphosphorylated protein is distributed throughout the cytoplasm (By similarity). Localized in the chromatoid bodies of round spermatids (PubMed:27149095).By similarity1 Publication
Cytosol
- cytosol Source: Reactome
Nucleus
- exon-exon junction complex Source: MGI
- nuclear chromosome, telomeric region Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: MGI
- supraspliceosomal complex Source: MGI
Other locations
- chromatin Source: HGNC-UCL
- cytoplasm Source: GO_Central
- P-body Source: UniProtKB-SubCell
- perinuclear region of cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPathology & Biotechi
Disruption phenotypei
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000080717 | 1 – 1124 | Regulator of nonsense transcripts 1Add BLAST | 1124 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 10 | PhosphoserineBy similarity | 1 | |
Modified residuei | 31 | PhosphoserineBy similarity | 1 | |
Modified residuei | 560 | PhosphoserineBy similarity | 1 | |
Modified residuei | 951 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1014 | Omega-N-methylarginineBy similarity | 1 | |
Modified residuei | 1084 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1102 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1105 | PhosphoserineCombined sources | 1 | |
Modified residuei | 1122 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q9EPU0 |
jPOSTi | Q9EPU0 |
PaxDbi | Q9EPU0 |
PeptideAtlasi | Q9EPU0 |
PRIDEi | Q9EPU0 |
PTM databases
iPTMneti | Q9EPU0 |
PhosphoSitePlusi | Q9EPU0 |
SwissPalmi | Q9EPU0 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000058301, Expressed in skin of back and 227 other tissues |
Genevisiblei | Q9EPU0, MM |
Interactioni
Subunit structurei
Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1.
Interacts with UPF2.
Interacts with UPF3A and UPF3B.
Interacts with EST1A.
Interacts with SLBP.
Interacts (when hyperphosphorylated) with PNRC2.
Interacts with AGO1 and AGO2.
Interacts with GSPT2.
Interacts with isoform 1 and isoform 5 of ADAR/ADAR1.
Interacts with SMG7.
Interacts with ZC3H12A; this interaction occurs in a mRNA translationally active- and termination-dependent manner and is essential for ZC3H12A-mediated degradation of target mRNAs.
Interacts with CPSF6.
Interacts with MOV10; the interaction is direct and RNA-dependent.
Interacts with SHFL; the interaction increases in the presence of RNA.
Interacts with UPF2 and DDX4; interactions are mediated by TDRD6 (PubMed:27149095).
By similarity1 PublicationBinary interactionsi
Q9EPU0
With | #Exp. | IntAct |
---|---|---|
Eri1 [Q7TMF2] | 2 | EBI-6876715,EBI-16026214 |
Protein-protein interaction databases
BioGRIDi | 202860, 49 interactors |
DIPi | DIP-60114N |
IntActi | Q9EPU0, 10 interactors |
MINTi | Q9EPU0 |
STRINGi | 10090.ENSMUSP00000075089 |
Miscellaneous databases
RNActi | Q9EPU0, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 410 | Sufficient for interaction with RENT2By similarityAdd BLAST | 410 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 1084 – 1085 | [ST]-Q motif 1 | 2 | |
Motifi | 1102 – 1103 | [ST]-Q motif 2 | 2 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 47 – 75 | Ala/Gly/Pro-richAdd BLAST | 29 | |
Compositional biasi | 1037 – 1124 | Gln/Ser-richAdd BLAST | 88 |
Domaini
Sequence similaritiesi
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 116 – 267 | UPF1-typeAdd BLAST | 152 |
Keywords - Domaini
Repeat, Zinc-fingerPhylogenomic databases
eggNOGi | KOG1802, Eukaryota |
GeneTreei | ENSGT00940000157413 |
HOGENOMi | CLU_001666_4_3_1 |
InParanoidi | Q9EPU0 |
OMAi | DMKLMHG |
OrthoDBi | 62494at2759 |
PhylomeDBi | Q9EPU0 |
TreeFami | TF300554 |
Family and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
InterProi | View protein in InterPro IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR040812, DUF5599 IPR006935, Helicase/UvrB_N IPR027417, P-loop_NTPase IPR018999, RNA-helicase_UPF1_UPF2-interct |
Pfami | View protein in Pfam PF13086, AAA_11, 1 hit PF13087, AAA_12, 1 hit PF18141, DUF5599, 1 hit PF04851, ResIII, 1 hit PF09416, UPF1_Zn_bind, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
s (2)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MSVEAYGPSS QTLTFLDTEE AELLGADTQG SEFEFTDFTL PSQTQTPPGG
60 70 80 90 100
PGGAGGPGGA GAGGAAGQLD AQVGPEGILQ NGAVDDSVAK TSQLLAELNF
110 120 130 140 150
EEDEEDTYYT KDLPVHACSY CGIHDPACVV YCNTSKKWFC NGRGNTSGSH
160 170 180 190 200
IVNHLVRAKC KEVTLHKDGP LGETVLECYN CGCRNVFLLG FIPAKADSVV
210 220 230 240 250
VLLCRQPCAS QSSLKDINWD SSQWQPLIQD RCFLSWLVKI PSEQEQLRAR
260 270 280 290 300
QITAQQINKL EELWKENPSA TLEDLEKPGV DEEPQHVLLR YEDAYQYQNI
310 320 330 340 350
FGPLVKLEAD YDKKLKESQT QDNITVRWDL GLNKKRIAFF TLPKTDSGNE
360 370 380 390 400
DLVIIWLRDM RLMQGDEICL RYKGDLAPLW KGIGHVIKVP DNYGDEIAIE
410 420 430 440 450
LRSSVGAPVE VTHNFQVDFV WKSTSFDRMQ SALKTFAVDE TSVSGYIYHK
460 470 480 490 500
LLGHEVEDVV IKCQLPKRFT AQGLPDLNHS QVYAVKTVLQ RPLSLIQGPP
510 520 530 540 550
GTGKTVTSAT IVYHLARQGN GPVLVCAPSN IAVDQLTEKI HQTGLKVVRL
560 570 580 590 600
CAKSREAIDS PVSFLALHNQ IRNMDSMPEL QKLQQLKDET GELSSADEKR
610 620 630 640 650
YRALKRTAER ELLMNADVIC CTCVGAGDPR LAKMQFRSIL IDESTQATEP
660 670 680 690 700
ECMVPVVLGA KQLILVGDHC QLGPVVMCKK AAKAGLSQSL FERLVVLGIR
710 720 730 740 750
PIRLQVQYRM HPALSAFPSN IFYEGSLQNG VTAADRVKKG FDFQWPQPDK
760 770 780 790 800
PMFFYVTQGQ EEIASSGTSY LNRTEAANVE KITTKLLKAG AKPDQIGIIT
810 820 830 840 850
PYEGQRSYLV QYMQFSGSLH TKLYQEVEIA SVDAFQGREK DFIILSCVRA
860 870 880 890 900
NEHQGIGFLN DPRRLNVALT RARYGVIIVG NPKALSKQPL WNHLLSYYKE
910 920 930 940 950
QKALVEGPLN NLRESLMQFS KPRKLVNTVN PGARFMTTAM YDAREAIIPG
960 970 980 990 1000
SVYDRSSQGR PSNMYFQTHD QISMISAGPS HVAAMNIPIP FNLVMPPMPP
1010 1020 1030 1040 1050
PGYFGQANGP AAGRGTPKTK TGRGGRQKNR FGLPGPSQTT LPNSQASQDV
1060 1070 1080 1090 1100
ASQPFSQGAL TQGYVSMSQP SQMSQPGLSQ PELSQDSYLG DEFKSQIDVA
1110 1120
LSQDSTYQGE RAYQHGGVTG LSQY
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 56 | G → S in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 58 | G → A in BAE28409 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 60 | A → P in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 71 | A → S in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 428 | R → K in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 439 | D → E in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 460 | V → F in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 495 | L → F in AAT46119 (Ref. 3) Curated | 1 | |
Sequence conflicti | 633 | K → E in BAE28409 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 820 | H → Y in BAE28409 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_025764 | 348 – 358 | Missing in isoform 2. 4 PublicationsAdd BLAST | 11 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF322655 mRNA Translation: AAG42830.1 AY597039, AY597038 Genomic DNA Translation: AAT46119.1 AK148196 mRNA Translation: BAE28409.1 BC030916 mRNA Translation: AAH30916.1 BC052149 mRNA Translation: AAH52149.1 BC056442 mRNA Translation: AAH56442.1 AF182947 mRNA Translation: AAK08652.1 |
CCDSi | CCDS52572.1 [Q9EPU0-1] CCDS90409.1 [Q9EPU0-2] |
RefSeqi | NP_001116301.1, NM_001122829.2 [Q9EPU0-1] NP_109605.2, NM_030680.3 [Q9EPU0-2] XP_006509667.1, XM_006509604.3 [Q9EPU0-1] XP_006509668.1, XM_006509605.3 [Q9EPU0-1] |
Genome annotation databases
Ensembli | ENSMUST00000075666; ENSMUSP00000075089; ENSMUSG00000058301 [Q9EPU0-1] ENSMUST00000215817; ENSMUSP00000148927; ENSMUSG00000058301 [Q9EPU0-2] |
GeneIDi | 19704 |
KEGGi | mmu:19704 |
UCSCi | uc009mab.2, mouse [Q9EPU0-1] uc012gfa.1, mouse [Q9EPU0-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF322655 mRNA Translation: AAG42830.1 AY597039, AY597038 Genomic DNA Translation: AAT46119.1 AK148196 mRNA Translation: BAE28409.1 BC030916 mRNA Translation: AAH30916.1 BC052149 mRNA Translation: AAH52149.1 BC056442 mRNA Translation: AAH56442.1 AF182947 mRNA Translation: AAK08652.1 |
CCDSi | CCDS52572.1 [Q9EPU0-1] CCDS90409.1 [Q9EPU0-2] |
RefSeqi | NP_001116301.1, NM_001122829.2 [Q9EPU0-1] NP_109605.2, NM_030680.3 [Q9EPU0-2] XP_006509667.1, XM_006509604.3 [Q9EPU0-1] XP_006509668.1, XM_006509605.3 [Q9EPU0-1] |
3D structure databases
SMRi | Q9EPU0 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 202860, 49 interactors |
DIPi | DIP-60114N |
IntActi | Q9EPU0, 10 interactors |
MINTi | Q9EPU0 |
STRINGi | 10090.ENSMUSP00000075089 |
PTM databases
iPTMneti | Q9EPU0 |
PhosphoSitePlusi | Q9EPU0 |
SwissPalmi | Q9EPU0 |
Proteomic databases
EPDi | Q9EPU0 |
jPOSTi | Q9EPU0 |
PaxDbi | Q9EPU0 |
PeptideAtlasi | Q9EPU0 |
PRIDEi | Q9EPU0 |
Protocols and materials databases
Antibodypediai | 15175, 316 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000075666; ENSMUSP00000075089; ENSMUSG00000058301 [Q9EPU0-1] ENSMUST00000215817; ENSMUSP00000148927; ENSMUSG00000058301 [Q9EPU0-2] |
GeneIDi | 19704 |
KEGGi | mmu:19704 |
UCSCi | uc009mab.2, mouse [Q9EPU0-1] uc012gfa.1, mouse [Q9EPU0-2] |
Organism-specific databases
CTDi | 5976 |
MGIi | MGI:107995, Upf1 |
Phylogenomic databases
eggNOGi | KOG1802, Eukaryota |
GeneTreei | ENSGT00940000157413 |
HOGENOMi | CLU_001666_4_3_1 |
InParanoidi | Q9EPU0 |
OMAi | DMKLMHG |
OrthoDBi | 62494at2759 |
PhylomeDBi | Q9EPU0 |
TreeFami | TF300554 |
Enzyme and pathway databases
Reactomei | R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC) R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC) |
Miscellaneous databases
BioGRID-ORCSi | 19704, 7 hits in 19 CRISPR screens |
ChiTaRSi | Upf1, mouse |
PROi | PR:Q9EPU0 |
RNActi | Q9EPU0, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000058301, Expressed in skin of back and 227 other tissues |
Genevisiblei | Q9EPU0, MM |
Family and domain databases
CDDi | cd18808, SF1_C_Upf1, 1 hit |
InterProi | View protein in InterPro IPR041679, DNA2/NAM7-like_C IPR041677, DNA2/NAM7_AAA_11 IPR040812, DUF5599 IPR006935, Helicase/UvrB_N IPR027417, P-loop_NTPase IPR018999, RNA-helicase_UPF1_UPF2-interct |
Pfami | View protein in Pfam PF13086, AAA_11, 1 hit PF13087, AAA_12, 1 hit PF18141, DUF5599, 1 hit PF04851, ResIII, 1 hit PF09416, UPF1_Zn_bind, 1 hit |
SUPFAMi | SSF52540, SSF52540, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | RENT1_MOUSE | |
Accessioni | Q9EPU0Primary (citable) accession number: Q9EPU0 Secondary accession number(s): Q3UG00 Q99PR4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | May 29, 2007 | |
Last modified: | December 2, 2020 | |
This is version 172 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families