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UniProtKB - Q9EPU0 (RENT1_MOUSE)

Entry version 172 (02 Dec 2020)
Sequence version 2 (29 May 2007)
Previous versions | rss
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Protein

Regulator of nonsense transcripts 1

Gene

Upf1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score:

Annotation score:5 out of 5

<p>The annotation score provides a heuristic measure of the annotation content of a UniProtKB entry or proteome. This score <strong>cannot</strong> be used as a measure of the accuracy of the annotation as we cannot define the 'correct annotation' for any given protein.<p><a href='/help/annotation_score' target='_top'>More...</a></p>-Experimental evidence at protein leveli <p>This indicates the type of evidence that supports the existence of the protein. Note that the 'protein existence' evidence does not give information on the accuracy or correctness of the sequence(s) displayed.<p><a href='/help/protein_existence' target='_top'>More...</a></p>

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

RNA-dependent helicase and ATPase required for nonsense-mediated decay (NMD) of mRNAs containing premature stop codons (PubMed:27149095). Is recruited to mRNAs upon translation termination and undergoes a cycle of phosphorylation and dephosphorylation; its phosphorylation appears to be a key step in NMD. Recruited by release factors to stalled ribosomes together with the SMG1C protein kinase complex to form the transient SURF (SMG1-UPF1-eRF1-eRF3) complex. In EJC-dependent NMD, the SURF complex associates with the exon junction complex (EJC) (located 50-55 or more nucleotides downstream from the termination codon) through UPF2 and allows the formation of an UPF1-UPF2-UPF3 surveillance complex which is believed to activate NMD. Phosphorylated UPF1 is recognized by EST1B/SMG5, SMG6 and SMG7 which are thought to provide a link to the mRNA degradation machinery involving exonucleolytic and endonucleolytic pathways, and to serve as adapters to protein phosphatase 2A (PP2A), thereby triggering UPF1 dephosphorylation. UPF1 can also activate NMD without UPF2 or UPF3, and in the absence of the NMD-enhancing downstream EJC indicative for alternative NMD pathways. Plays a role in replication-dependent histone mRNA degradation at the end of phase S; the function is independent of UPF2. For the recognition of premature termination codons (PTC) and initiation of NMD a competitive interaction between UPF1 and PABPC1 with the ribosome-bound release factors is proposed. The ATPase activity of UPF1 is required for disassembly of mRNPs undergoing NMD (By similarity). Essential for embryonic viability (By similarity). Together with UPF2 and dependent on TDRD6, mediates the degradation of mRNA hardoring long 3'UTR by inducing the NMD machinery (PubMed:27149095).By similarity1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes the interaction between a single amino acid and another chemical entity. Priority is given to the annotation of physiological ligands.<p><a href='/help/binding' target='_top'>More...</a></p>Binding sitei481ATPBy similarity1
Binding sitei671ATPBy similarity1
Binding sitei708ATPBy similarity1
Binding sitei839ATPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section specifies the position(s) and type(s) of zinc fingers within the protein.<p><a href='/help/zn_fing' target='_top'>More...</a></p>Zinc fingeri116 – 267UPF1-typeAdd BLAST152
<p>This subsection of the <a href="http://www.uniprot.org/help/function%5Fsection">Function</a> section describes a region in the protein which binds nucleotide phosphates. It always involves more than one amino acid and includes all residues involved in nucleotide-binding.<p><a href='/help/np_bind' target='_top'>More...</a></p>Nucleotide bindingi501 – 505ATPBy similarity5

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

Complete GO annotation on QuickGO ...

GO - Biological processi

Complete GO annotation on QuickGO ...

<p>UniProtKB Keywords constitute a <a href="http://www.uniprot.org/keywords">controlled vocabulary</a> with a hierarchical structure. Keywords summarise the content of a UniProtKB entry and facilitate the search for proteins of interest.<p><a href='/help/keywords' target='_top'>More...</a></p>Keywordsi

Molecular functionHelicase, Hydrolase, RNA-binding
Biological processNonsense-mediated mRNA decay
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

Reactome - a knowledgebase of biological pathways and processes

More...Reactomei		R-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides an exhaustive list of all names of the protein, from commonly used to obsolete, to allow unambiguous identification of a protein.<p><a href='/help/protein_names' target='_top'>More...</a></p>Protein namesi
Recommended name:
Regulator of nonsense transcripts 1 (EC:3.6.4.-)
Alternative name(s):
ATP-dependent helicase RENT1
Nonsense mRNA reducing factor 1
Short name:
NORF1
Up-frameshift suppressor 1 homolog
Short name:
mUpf1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section indicates the name(s) of the gene(s) that code for the protein sequence(s) described in the entry. Four distinct tokens exist: 'Name', 'Synonyms', 'Ordered locus names' and 'ORF names'.<p><a href='/help/gene_name' target='_top'>More...</a></p>Gene namesi
Name:Upf1
Synonyms:Rent1
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section provides information on the name(s) of the organism that is the source of the protein sequence.<p><a href='/help/organism-name' target='_top'>More...</a></p>OrganismiMus musculus (Mouse)
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section shows the unique identifier assigned by the NCBI to the source organism of the protein. This is known as the 'taxonomic identifier' or 'taxid'.<p><a href='/help/taxonomic_identifier' target='_top'>More...</a></p>Taxonomic identifieri10090 [NCBI]
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section contains the taxonomic hierarchical classification lineage of the source organism. It lists the nodes as they appear top-down in the taxonomic tree, with the more general grouping listed first.<p><a href='/help/taxonomic_lineage' target='_top'>More...</a></p>Taxonomic lineageiEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
<p>This subsection of the <a href="http://www.uniprot.org/help/names%5Fand%5Ftaxonomy%5Fsection">Names and taxonomy</a> section is present for entries that are part of a <a href="http://www.uniprot.org/proteomes">proteome</a>, i.e. of a set of proteins thought to be expressed by organisms whose genomes have been completely sequenced.<p><a href='/help/proteomes_manual' target='_top'>More...</a></p>Proteomesi
  • UP000000589 <p>A UniProt <a href="http://www.uniprot.org/manual/proteomes%5Fmanual">proteome</a> can consist of several components.<br></br>The component name refers to the genomic component encoding a set of proteins.<p><a href='/help/proteome_component' target='_top'>More...</a></p> Componenti: Chromosome 8

Organism-specific databases

Mouse genome database (MGD) from Mouse Genome Informatics (MGI)

More...MGIi		MGI:107995, Upf1

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte & Seán O’Donoghue; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

<p>This subsection of the 'Pathology and Biotech' section describes the in vivo effects caused by ablation of the gene (or one or more transcripts) coding for the protein described in the entry. This includes gene knockout and knockdown, provided experiments have been performed in the context of a whole organism or a specific tissue, and not at the single-cell level.<p><a href='/help/disruption_phenotype' target='_top'>More...</a></p>Disruption phenotypei

Embryos are viable in pre-implantation period, show complete loss of NMD but are resorbed shortly after implantation.1 Publication

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section describes the extent of a polypeptide chain in the mature protein following processing or proteolytic cleavage.<p><a href='/help/chain' target='_top'>More...</a></p>ChainiPRO_00000807171 – 1124Regulator of nonsense transcripts 1Add BLAST1124

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'PTM / Processing' section specifies the position and type of each modified residue excluding <a href="http://www.uniprot.org/manual/lipid">lipids</a>, <a href="http://www.uniprot.org/manual/carbohyd">glycans</a> and <a href="http://www.uniprot.org/manual/crosslnk">protein cross-links</a>.<p><a href='/help/mod_res' target='_top'>More...</a></p>Modified residuei10PhosphoserineBy similarity1
Modified residuei31PhosphoserineBy similarity1
Modified residuei560PhosphoserineBy similarity1
Modified residuei951PhosphoserineBy similarity1
Modified residuei1014Omega-N-methylarginineBy similarity1
Modified residuei1084PhosphoserineBy similarity1
Modified residuei1102PhosphoserineCombined sources1
Modified residuei1105PhosphoserineCombined sources1
Modified residuei1122PhosphoserineBy similarity1

<p>This subsection of the <a href="http://www.uniprot.org/help/ptm%5Fprocessing%5Fsection">PTM/processing</a> section describes post-translational modifications (PTMs). This subsection <strong>complements</strong> the information provided at the sequence level or describes modifications for which <strong>position-specific data is not yet available</strong>.<p><a href='/help/post-translational_modification' target='_top'>More...</a></p>Post-translational modificationi

Phosphorylated by SMG1; required for formation of mRNA surveillance complexes.By similarity

Keywords - PTMi

Methylation, Phosphoprotein

Proteomic databases

Encyclopedia of Proteome Dynamics

More...EPDi		Q9EPU0

jPOST - Japan Proteome Standard Repository/Database

More...jPOSTi		Q9EPU0

PaxDb, a database of protein abundance averages across all three domains of life

More...PaxDbi		Q9EPU0

PeptideAtlas

More...PeptideAtlasi		Q9EPU0

PRoteomics IDEntifications database

More...PRIDEi		Q9EPU0

PTM databases

iPTMnet integrated resource for PTMs in systems biology context

More...iPTMneti		Q9EPU0

Comprehensive resource for the study of protein post-translational modifications (PTMs) in human, mouse and rat.

More...PhosphoSitePlusi		Q9EPU0

SwissPalm database of S-palmitoylation events

More...SwissPalmi		Q9EPU0

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

<p>This subsection of the 'Expression' section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms. By default, the information is derived from experiments at the mRNA level, unless specified 'at protein level'.<br></br>Examples: <a href="http://www.uniprot.org/uniprot/P92958#expression">P92958</a>, <a href="http://www.uniprot.org/uniprot/Q8TDN4#expression">Q8TDN4</a>, <a href="http://www.uniprot.org/uniprot/O14734#expression">O14734</a><p><a href='/help/tissue_specificity' target='_top'>More...</a></p>Tissue specificityi

Localizes in male germ cells.1 Publication

<p>This subsection of the 'Expression' section provides information on the expression of the gene product at various stages of a cell, tissue or organism development. By default, the information is derived from experiments at the mRNA level, unless specified 'at the protein level'.<p><a href='/help/developmental_stage' target='_top'>More...</a></p>Developmental stagei

Weakly expressed in neonatal testes and expression increases during the development of spermatocytes and spermatids, in the late meiotic and postmeiotic stages of spermatogenesis.1 Publication

Gene expression databases

Bgee dataBase for Gene Expression Evolution

More...Bgeei		ENSMUSG00000058301, Expressed in skin of back and 227 other tissues

Genevisible search portal to normalized and curated expression data from Genevestigator

More...Genevisiblei		Q9EPU0, MM

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

<p>This subsection of the <a href="http://www.uniprot.org/help/interaction%5Fsection">'Interaction'</a> section provides information about the protein quaternary structure and interaction(s) with other proteins or protein complexes (with the exception of physiological receptor-ligand interactions which are annotated in the <a href="http://www.uniprot.org/help/function%5Fsection">'Function'</a> section).<p><a href='/help/subunit_structure' target='_top'>More...</a></p>Subunit structurei

Found in a post-splicing messenger ribonucleoprotein (mRNP) complex. Associates with the exon junction complex (EJC). Associates with the SGM1C complex; is phosphorylated by the complex kinase component SGM1.

Interacts with UPF2.

Interacts with UPF3A and UPF3B.

Interacts with EST1A.

Interacts with SLBP.

Interacts (when hyperphosphorylated) with PNRC2.

Interacts with AGO1 and AGO2.

Interacts with GSPT2.

Interacts with isoform 1 and isoform 5 of ADAR/ADAR1.

Interacts with SMG7.

Interacts with ZC3H12A; this interaction occurs in a mRNA translationally active- and termination-dependent manner and is essential for ZC3H12A-mediated degradation of target mRNAs.

Interacts with CPSF6.

Interacts with MOV10; the interaction is direct and RNA-dependent.

Interacts with SHFL; the interaction increases in the presence of RNA.

Interacts with UPF2 and DDX4; interactions are mediated by TDRD6 (PubMed:27149095).

By similarity1 Publication

<p>This subsection of the '<a href="http://www.uniprot.org/help/interaction%5Fsection">Interaction</a>' section provides information about binary protein-protein interactions. The data presented in this section are a quality-filtered subset of binary interactions automatically derived from the <a href="https://www.ebi.ac.uk/intact/">IntAct database</a>. It is updated at every <a href="http://www.uniprot.org/help/synchronization">UniProt release</a>.<p><a href='/help/binary_interactions' target='_top'>More...</a></p>Binary interactionsi

Protein-protein interaction databases

The Biological General Repository for Interaction Datasets (BioGRID)

More...BioGRIDi		202860, 49 interactors

Database of interacting proteins

More...DIPi		DIP-60114N

Protein interaction database and analysis system

More...IntActi		Q9EPU0, 10 interactors

Molecular INTeraction database

More...MINTi		Q9EPU0

STRING: functional protein association networks

More...STRINGi		10090.ENSMUSP00000075089

Miscellaneous databases

RNAct, Protein-RNA interaction predictions for model organisms.

More...RNActi		Q9EPU0, protein

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

SWISS-MODEL Repository - a database of annotated 3D protein structure models

More...SMRi		Q9EPU0

Database of comparative protein structure models

More...ModBasei		Search...

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a region of interest that cannot be described in other subsections.<p><a href='/help/region' target='_top'>More...</a></p>Regioni1 – 410Sufficient for interaction with RENT2By similarityAdd BLAST410

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes a short (usually not more than 20 amino acids) conserved sequence motif of biological significance.<p><a href='/help/motif' target='_top'>More...</a></p>Motifi1084 – 1085[ST]-Q motif 12
Motifi1102 – 1103[ST]-Q motif 22

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Family and Domains' section describes the position of regions of compositional bias within the protein and the particular amino acids that are over-represented within those regions.<p><a href='/help/compbias' target='_top'>More...</a></p>Compositional biasi47 – 75Ala/Gly/Pro-richAdd BLAST29
Compositional biasi1037 – 1124Gln/Ser-richAdd BLAST88

<p>This subsection of the 'Family and domains' section provides general information on the biological role of a domain. The term 'domain' is intended here in its wide acceptation, it may be a structural domain, a transmembrane region or a functional domain. Several domains are described in this subsection.<p><a href='/help/domain_cc' target='_top'>More...</a></p>Domaini

The [ST]-Q motif constitutes a recognition sequence for kinases from the PI3/PI4-kinase family.By similarity

<p>This subsection of the 'Family and domains' section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Belongs to the DNA2/NAM7 helicase family.Curated

Zinc finger

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Zinc fingeri116 – 267UPF1-typeAdd BLAST152

Keywords - Domaini

Repeat, Zinc-finger

Phylogenomic databases

evolutionary genealogy of genes: Non-supervised Orthologous Groups

More...eggNOGi		KOG1802, Eukaryota

Ensembl GeneTree

More...GeneTreei		ENSGT00940000157413

The HOGENOM Database of Homologous Genes from Fully Sequenced Organisms

More...HOGENOMi		CLU_001666_4_3_1

InParanoid: Eukaryotic Ortholog Groups

More...InParanoidi		Q9EPU0

Identification of Orthologs from Complete Genome Data

More...OMAi		DMKLMHG

Database of Orthologous Groups

More...OrthoDBi		62494at2759

Database for complete collections of gene phylogenies

More...PhylomeDBi		Q9EPU0

TreeFam database of animal gene trees

More...TreeFami		TF300554

Family and domain databases

Conserved Domains Database

More...CDDi		cd18808, SF1_C_Upf1, 1 hit

Integrated resource of protein families, domains and functional sites

More...InterProi		View protein in InterPro
IPR041679, DNA2/NAM7-like_C
IPR041677, DNA2/NAM7_AAA_11
IPR040812, DUF5599
IPR006935, Helicase/UvrB_N
IPR027417, P-loop_NTPase
IPR018999, RNA-helicase_UPF1_UPF2-interct

Pfam protein domain database

More...Pfami		View protein in Pfam
PF13086, AAA_11, 1 hit
PF13087, AAA_12, 1 hit
PF18141, DUF5599, 1 hit
PF04851, ResIII, 1 hit
PF09416, UPF1_Zn_bind, 1 hit

Superfamily database of structural and functional annotation

More...SUPFAMi		SSF52540, SSF52540, 1 hit

<p>This section displays by default the canonical protein sequence and upon request all isoforms described in the entry. It also includes information pertinent to the sequence(s), including <a href="http://www.uniprot.org/help/sequence%5Flength">length</a> and <a href="http://www.uniprot.org/help/sequences">molecular weight</a>. The information is filed in different subsections. The current subsections and their content are listed below:<p><a href='/help/sequences_section' target='_top'>More...</a></p>Sequences (2)i

<p>This subsection of the <a href="http://www.uniprot.org/help/sequences%5Fsection">Sequence</a> section indicates if the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> displayed by default in the entry is complete or not.<p><a href='/help/sequence_status' target='_top'>More...</a></p>Sequence statusi: Complete.

This entry describes 2 <p>This subsection of the 'Sequence' section lists the alternative protein sequences (isoforms) that can be generated from the same gene by a single or by the combination of up to four biological events (alternative promoter usage, alternative splicing, alternative initiation and ribosomal frameshifting). Additionally, this section gives relevant information on each alternative protein isoform. This section is only present in reviewed entries, i.e. in UniProtKB/Swiss-Prot.<p><a href='/help/alternative_products' target='_top'>More...</a></p> isoformsi produced by alternative splicing. AlignAdd to basket
Isoform 1 (identifier: Q9EPU0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the <div> <p><b>What is the canonical sequence?</b><p><a href='/help/canonical_and_isoforms' target='_top'>More...</a></p>canonicali sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide
        10         20         30         40         50
MSVEAYGPSS QTLTFLDTEE AELLGADTQG SEFEFTDFTL PSQTQTPPGG 
        60         70         80         90        100
PGGAGGPGGA GAGGAAGQLD AQVGPEGILQ NGAVDDSVAK TSQLLAELNF 
       110        120        130        140        150
EEDEEDTYYT KDLPVHACSY CGIHDPACVV YCNTSKKWFC NGRGNTSGSH 
       160        170        180        190        200
IVNHLVRAKC KEVTLHKDGP LGETVLECYN CGCRNVFLLG FIPAKADSVV 
       210        220        230        240        250
VLLCRQPCAS QSSLKDINWD SSQWQPLIQD RCFLSWLVKI PSEQEQLRAR 
       260        270        280        290        300
QITAQQINKL EELWKENPSA TLEDLEKPGV DEEPQHVLLR YEDAYQYQNI 
       310        320        330        340        350
FGPLVKLEAD YDKKLKESQT QDNITVRWDL GLNKKRIAFF TLPKTDSGNE 
       360        370        380        390        400
DLVIIWLRDM RLMQGDEICL RYKGDLAPLW KGIGHVIKVP DNYGDEIAIE 
       410        420        430        440        450
LRSSVGAPVE VTHNFQVDFV WKSTSFDRMQ SALKTFAVDE TSVSGYIYHK 
       460        470        480        490        500
LLGHEVEDVV IKCQLPKRFT AQGLPDLNHS QVYAVKTVLQ RPLSLIQGPP 
       510        520        530        540        550
GTGKTVTSAT IVYHLARQGN GPVLVCAPSN IAVDQLTEKI HQTGLKVVRL 
       560        570        580        590        600
CAKSREAIDS PVSFLALHNQ IRNMDSMPEL QKLQQLKDET GELSSADEKR 
       610        620        630        640        650
YRALKRTAER ELLMNADVIC CTCVGAGDPR LAKMQFRSIL IDESTQATEP 
       660        670        680        690        700
ECMVPVVLGA KQLILVGDHC QLGPVVMCKK AAKAGLSQSL FERLVVLGIR 
       710        720        730        740        750
PIRLQVQYRM HPALSAFPSN IFYEGSLQNG VTAADRVKKG FDFQWPQPDK 
       760        770        780        790        800
PMFFYVTQGQ EEIASSGTSY LNRTEAANVE KITTKLLKAG AKPDQIGIIT 
       810        820        830        840        850
PYEGQRSYLV QYMQFSGSLH TKLYQEVEIA SVDAFQGREK DFIILSCVRA 
       860        870        880        890        900
NEHQGIGFLN DPRRLNVALT RARYGVIIVG NPKALSKQPL WNHLLSYYKE 
       910        920        930        940        950
QKALVEGPLN NLRESLMQFS KPRKLVNTVN PGARFMTTAM YDAREAIIPG 
       960        970        980        990       1000
SVYDRSSQGR PSNMYFQTHD QISMISAGPS HVAAMNIPIP FNLVMPPMPP 
      1010       1020       1030       1040       1050
PGYFGQANGP AAGRGTPKTK TGRGGRQKNR FGLPGPSQTT LPNSQASQDV 
      1060       1070       1080       1090       1100
ASQPFSQGAL TQGYVSMSQP SQMSQPGLSQ PELSQDSYLG DEFKSQIDVA 
      1110       1120 
LSQDSTYQGE RAYQHGGVTG LSQY
Length:1,124
Mass (Da):123,967
Last modified:May 29, 2007 - v2
<p>The checksum is a form of redundancy check that is calculated from the sequence. It is useful for tracking sequence updates.</p> <p>It should be noted that while, in theory, two different sequences could have the same checksum value, the likelihood that this would happen is extremely low.</p> <p>However UniProtKB may contain entries with identical sequences in case of multiple genes (paralogs).</p> <p>The checksum is computed as the sequence 64-bit Cyclic Redundancy Check value (CRC64) using the generator polynomial: x<sup>64</sup> + x<sup>4</sup> + x<sup>3</sup> + x + 1. The algorithm is described in the ISO 3309 standard. </p> <p class="publication">Press W.H., Flannery B.P., Teukolsky S.A. and Vetterling W.T.<br /> <strong>Cyclic redundancy and other checksums</strong><br /> <a href="http://www.nrbook.com/b/bookcpdf.php">Numerical recipes in C 2nd ed., pp896-902, Cambridge University Press (1993)</a>)</p> Checksum:i5F1B0ED50F63C52E
GO
Isoform 2 (identifier: Q9EPU0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     348-358: Missing.

Show »
Length:1,113
Mass (Da):122,657
Checksum:iBFE8D50AD49DAA54
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section reports difference(s) between the canonical sequence (displayed by default in the entry) and the different sequence submissions merged in the entry. These various submissions may originate from different sequencing projects, different types of experiments, or different biological samples. Sequence conflicts are usually of unknown origin.<p><a href='/help/conflict' target='_top'>More...</a></p>Sequence conflicti56G → S in AAT46119 (Ref. 3) Curated1
Sequence conflicti58G → A in BAE28409 (PubMed:16141072).Curated1
Sequence conflicti60A → P in AAT46119 (Ref. 3) Curated1
Sequence conflicti71A → S in AAT46119 (Ref. 3) Curated1
Sequence conflicti428R → K in AAT46119 (Ref. 3) Curated1
Sequence conflicti439D → E in AAT46119 (Ref. 3) Curated1
Sequence conflicti460V → F in AAT46119 (Ref. 3) Curated1
Sequence conflicti495L → F in AAT46119 (Ref. 3) Curated1
Sequence conflicti633K → E in BAE28409 (PubMed:16141072).Curated1
Sequence conflicti820H → Y in BAE28409 (PubMed:16141072).Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
<p>This subsection of the 'Sequence' section describes the sequence of naturally occurring alternative protein isoform(s). The changes in the amino acid sequence may be due to alternative splicing, alternative promoter usage, alternative initiation, or ribosomal frameshifting.<p><a href='/help/var_seq' target='_top'>More...</a></p>Alternative sequenceiVSP_025764348 – 358Missing in isoform 2. 4 PublicationsAdd BLAST11

Sequence databases

Select the link destinations:

EMBL nucleotide sequence database

More...EMBLi

GenBank nucleotide sequence database

More...GenBanki

DNA Data Bank of Japan; a nucleotide sequence database

More...DDBJi
Links Updated
AF322655 mRNA Translation: AAG42830.1
AY597039, AY597038 Genomic DNA Translation: AAT46119.1
AK148196 mRNA Translation: BAE28409.1
BC030916 mRNA Translation: AAH30916.1
BC052149 mRNA Translation: AAH52149.1
BC056442 mRNA Translation: AAH56442.1
AF182947 mRNA Translation: AAK08652.1

The Consensus CDS (CCDS) project

More...CCDSi		CCDS52572.1 [Q9EPU0-1]
CCDS90409.1 [Q9EPU0-2]

NCBI Reference Sequences

More...RefSeqi		NP_001116301.1, NM_001122829.2 [Q9EPU0-1]
NP_109605.2, NM_030680.3 [Q9EPU0-2]
XP_006509667.1, XM_006509604.3 [Q9EPU0-1]
XP_006509668.1, XM_006509605.3 [Q9EPU0-1]

Genome annotation databases

Ensembl eukaryotic genome annotation project

More...Ensembli		ENSMUST00000075666; ENSMUSP00000075089; ENSMUSG00000058301 [Q9EPU0-1]
ENSMUST00000215817; ENSMUSP00000148927; ENSMUSG00000058301 [Q9EPU0-2]

Database of genes from NCBI RefSeq genomes

More...GeneIDi		19704

KEGG: Kyoto Encyclopedia of Genes and Genomes

More...KEGGi		mmu:19704

UCSC genome browser

More...UCSCi		uc009mab.2, mouse [Q9EPU0-1]
uc012gfa.1, mouse [Q9EPU0-2]

Keywords - Coding sequence diversityi

Alternative splicing

<p>This section provides links to proteins that are similar to the protein sequence(s) described in this entry at different levels of sequence identity thresholds (100%, 90% and 50%) based on their membership in UniProt Reference Clusters (<a href="http://www.uniprot.org/help/uniref">UniRef</a>).<p><a href='/help/similar_proteins_section' target='_top'>More...</a></p>Similar proteinsi

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF322655 mRNA Translation: AAG42830.1
AY597039, AY597038 Genomic DNA Translation: AAT46119.1
AK148196 mRNA Translation: BAE28409.1
BC030916 mRNA Translation: AAH30916.1
BC052149 mRNA Translation: AAH52149.1
BC056442 mRNA Translation: AAH56442.1
AF182947 mRNA Translation: AAK08652.1
CCDSiCCDS52572.1 [Q9EPU0-1]
CCDS90409.1 [Q9EPU0-2]
RefSeqiNP_001116301.1, NM_001122829.2 [Q9EPU0-1]
NP_109605.2, NM_030680.3 [Q9EPU0-2]
XP_006509667.1, XM_006509604.3 [Q9EPU0-1]
XP_006509668.1, XM_006509605.3 [Q9EPU0-1]

3D structure databases

SMRiQ9EPU0
ModBaseiSearch...

Protein-protein interaction databases

BioGRIDi202860, 49 interactors
DIPiDIP-60114N
IntActiQ9EPU0, 10 interactors
MINTiQ9EPU0
STRINGi10090.ENSMUSP00000075089

PTM databases

iPTMnetiQ9EPU0
PhosphoSitePlusiQ9EPU0
SwissPalmiQ9EPU0

Proteomic databases

EPDiQ9EPU0
jPOSTiQ9EPU0
PaxDbiQ9EPU0
PeptideAtlasiQ9EPU0
PRIDEiQ9EPU0

Protocols and materials databases

Antibodypedia a portal for validated antibodies

More...Antibodypediai		15175, 316 antibodies

Genome annotation databases

EnsembliENSMUST00000075666; ENSMUSP00000075089; ENSMUSG00000058301 [Q9EPU0-1]
ENSMUST00000215817; ENSMUSP00000148927; ENSMUSG00000058301 [Q9EPU0-2]
GeneIDi19704
KEGGimmu:19704
UCSCiuc009mab.2, mouse [Q9EPU0-1]
uc012gfa.1, mouse [Q9EPU0-2]

Organism-specific databases

Comparative Toxicogenomics Database

More...CTDi		5976
MGIiMGI:107995, Upf1

Phylogenomic databases

eggNOGiKOG1802, Eukaryota
GeneTreeiENSGT00940000157413
HOGENOMiCLU_001666_4_3_1
InParanoidiQ9EPU0
OMAiDMKLMHG
OrthoDBi62494at2759
PhylomeDBiQ9EPU0
TreeFamiTF300554

Enzyme and pathway databases

ReactomeiR-MMU-975956, Nonsense Mediated Decay (NMD) independent of the Exon Junction Complex (EJC)
R-MMU-975957, Nonsense Mediated Decay (NMD) enhanced by the Exon Junction Complex (EJC)

Miscellaneous databases

BioGRID ORCS database of CRISPR phenotype screens

More...BioGRID-ORCSi		19704, 7 hits in 19 CRISPR screens

ChiTaRS: a database of human, mouse and fruit fly chimeric transcripts and RNA-sequencing data

More...ChiTaRSi		Upf1, mouse

Protein Ontology

More...PROi		PR:Q9EPU0
RNActiQ9EPU0, protein

The Stanford Online Universal Resource for Clones and ESTs

More...SOURCEi		Search...

Gene expression databases

BgeeiENSMUSG00000058301, Expressed in skin of back and 227 other tissues
GenevisibleiQ9EPU0, MM

Family and domain databases

CDDicd18808, SF1_C_Upf1, 1 hit
InterProiView protein in InterPro
IPR041679, DNA2/NAM7-like_C
IPR041677, DNA2/NAM7_AAA_11
IPR040812, DUF5599
IPR006935, Helicase/UvrB_N
IPR027417, P-loop_NTPase
IPR018999, RNA-helicase_UPF1_UPF2-interct
PfamiView protein in Pfam
PF13086, AAA_11, 1 hit
PF13087, AAA_12, 1 hit
PF18141, DUF5599, 1 hit
PF04851, ResIII, 1 hit
PF09416, UPF1_Zn_bind, 1 hit
SUPFAMiSSF52540, SSF52540, 1 hit

ProtoNet; Automatic hierarchical classification of proteins

More...ProtoNeti		Search...

MobiDB: a database of protein disorder and mobility annotations

More...MobiDBi		Search...

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This subsection of the 'Entry information' section provides a mnemonic identifier for a UniProtKB entry, but it is not a stable identifier. Each reviewed entry is assigned a unique entry name upon integration into UniProtKB/Swiss-Prot.<p><a href='/help/entry_name' target='_top'>More...</a></p>Entry nameiRENT1_MOUSE
<p>This subsection of the 'Entry information' section provides one or more accession number(s). These are stable identifiers and should be used to cite UniProtKB entries. Upon integration into UniProtKB, each entry is assigned a unique accession number, which is called 'Primary (citable) accession number'.<p><a href='/help/accession_numbers' target='_top'>More...</a></p>AccessioniPrimary (citable) accession number: Q9EPU0
Secondary accession number(s): Q3UG00
, Q6GYP5, Q6PHQ5, Q8K0N4, Q99PR4
<p>This subsection of the 'Entry information' section shows the date of integration of the entry into UniProtKB, the date of the last sequence update and the date of the last annotation modification ('Last modified'). The version number for both the entry and the <a href="http://www.uniprot.org/help/canonical%5Fand%5Fisoforms">canonical sequence</a> are also displayed.<p><a href='/help/entry_history' target='_top'>More...</a></p>Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 18, 2001
Last sequence update: May 29, 2007
Last modified: December 2, 2020
This is version 172 of the entry and version 2 of the sequence. See complete history.
<p>This subsection of the 'Entry information' section indicates whether the entry has been manually annotated and reviewed by UniProtKB curators or not, in other words, if the entry belongs to the Swiss-Prot section of UniProtKB (<strong>reviewed</strong>) or to the computer-annotated TrEMBL section (<strong>unreviewed</strong>).<p><a href='/help/entry_status' target='_top'>More...</a></p>Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

<p>This section contains any relevant information that doesn't fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families
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