UniProtKB - Q9EPL8 (IPO7_MOUSE)
Protein
Importin-7
Gene
Ipo7
Organism
Mus musculus (Mouse)
Status
Functioni
Functions in nuclear protein import, either by acting as autonomous nuclear transport receptor or as an adapter-like protein in association with the importin-beta subunit KPNB1. Acting autonomously is thought to serve itself as receptor for nuclear localization signals (NLS) and to promote translocation of import substrates through the nuclear pore complex (NPC) by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin, the importin/substrate complex dissociates and importin is re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with KPNB1 mediates the nuclear import of H1 histone and the Ran-binding site of IPO7 is not required but synergizes with that of KPNB1 in importin/substrate complex dissociation (By similarity). In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones.By similarity1 Publication
GO - Molecular functioni
- histone binding Source: MGI
- Ran GTPase binding Source: InterPro
- SMAD binding Source: MGI
GO - Biological processi
- innate immune response Source: MGI
- protein import into nucleus Source: MGI
Keywordsi
Biological process | Protein transport, Transport |
Names & Taxonomyi
Protein namesi | Recommended name: Importin-7Short name: Imp7 Alternative name(s): Ran-binding protein 7 Short name: RanBP7 |
Gene namesi | Name:Ipo7 Synonyms:Ranbp7 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2152414, Ipo7 |
Subcellular locationi
Cytosol
- cytosol Source: MGI
Nucleus
- nuclear envelope Source: GO_Central
- nucleoplasm Source: MGI
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000120751 | 1 – 1038 | Importin-7Add BLAST | 1038 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 1 | N-acetylmethionineBy similarity | 1 | |
Modified residuei | 886 | PhosphoserineBy similarity | 1 | |
Modified residuei | 898 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 903 | PhosphoserineBy similarity | 1 | |
Modified residuei | 1020 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9EPL8 |
jPOSTi | Q9EPL8 |
MaxQBi | Q9EPL8 |
PaxDbi | Q9EPL8 |
PeptideAtlasi | Q9EPL8 |
PRIDEi | Q9EPL8 |
PTM databases
iPTMneti | Q9EPL8 |
PhosphoSitePlusi | Q9EPL8 |
SwissPalmi | Q9EPL8 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000066232, Expressed in parotid gland and 311 other tissues |
Genevisiblei | Q9EPL8, MM |
Interactioni
Subunit structurei
Forms a heterodimer with KPNB1.
Interacts with KPNB1, RNUT1, XPO1, RPL23A, RPS7 and RPL5. Binds directly to nuclear pore complexes (By similarity).
Interacts with H2A, H2B, H3 and H4 histones.
Interacts with SMAD4 and NUP93; translocates SMAD4 to the nucleus through the NPC upon BMP7 stimulation resulting in activation of SMAD4 signaling (By similarity).
By similarity1 PublicationGO - Molecular functioni
- histone binding Source: MGI
- Ran GTPase binding Source: InterPro
- SMAD binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 231437, 18 interactors |
IntActi | Q9EPL8, 6 interactors |
MINTi | Q9EPL8 |
STRINGi | 10090.ENSMUSP00000081782 |
Miscellaneous databases
RNActi | Q9EPL8, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 101 | Importin N-terminalPROSITE-ProRule annotationAdd BLAST | 80 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 886 – 958 | Asp-richAdd BLAST | 73 |
Sequence similaritiesi
Belongs to the importin beta family.Curated
Phylogenomic databases
eggNOGi | KOG1991, Eukaryota |
GeneTreei | ENSGT00940000154666 |
HOGENOMi | CLU_004196_1_1_1 |
InParanoidi | Q9EPL8 |
OMAi | VKPKSTW |
OrthoDBi | 159062at2759 |
PhylomeDBi | Q9EPL8 |
TreeFami | TF300634 |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR013713, XPO2_central |
Pfami | View protein in Pfam PF08506, Cse1, 1 hit PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9EPL8-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MDPNTIIEAL RGTMDPALRE AAERQLNEAH KSLNFVSTLL QITMSEQLDL
60 70 80 90 100
PVRQAGVIYL KNMITQYWPD REATPGDIAP YTIPEEDRHC IRENIVEAII
110 120 130 140 150
HSPELIRVQL TTCIHHIIKH DYPSRWTAIV DKIGFYLQSD NSACWLGILL
160 170 180 190 200
CLYQLVKNYE YKKPEERSPL VAAMQHFLPV LKDRFIQLLS DQSDQSVLIQ
210 220 230 240 250
KQIFKIFYAL VQYTLPLELI NQQNLTEWVE ILKTVVNRDV PNETLQVEED
260 270 280 290 300
DRPELPWWKC KKWALHILAR LFERYGSPGN VSKEYNEFAE VFLKAFAVGV
310 320 330 340 350
QQVLLKVLYQ YKEKQYMAPR VLQQTLNYIN QGVSHALTWK NLKPHIQGII
360 370 380 390 400
QDVIFPLMCY TDADEELWQE DPYEYIRMKF DVFEDFISPT TAAQTLLFTA
410 420 430 440 450
CSKRKEVLQK TMGFCYQILT EPNADPRKKD GALHMIGSLA EILLKKKIYK
460 470 480 490 500
DQMEYMLQNH VFPLFSSELG YMRARACWVL HYFCEVKFKS DQNLQTALEL
510 520 530 540 550
TRRCLIDDRE MPVKVEAAIA LQVLISNQEK AKEYITPFIR PVMQALLHII
560 570 580 590 600
RETENDDLTN VIQKMICEYS EEVTPIAVEM TQHLAMTFNQ VIQTGPDEEG
610 620 630 640 650
SDDKAVTAMG ILNTIDTLLS VVEDHKEITQ QLEGICLQVI GTVLQQHVLE
660 670 680 690 700
FYEEIFSLAH SLTCQQVSPQ MWQLLPLVFE VFQQDGFDYF TDMMPLLHNY
710 720 730 740 750
VTVDTDTLLS DTKYLEMIYS MCKKVLTGVA GEDAECHAAK LLEVIILQCK
760 770 780 790 800
GRGIDQCIPL FVEAALERLT REVKTSELRT MCLQVAIAAL YYNPHLLLNT
810 820 830 840 850
LENLRFPNNV EPVTNHFITQ WLNDVDCFLG LHDRKMCVLG LCALIDMEQI
860 870 880 890 900
PQVLNQVSGQ ILPAFILLFN GLKRAYACHA EHENDSDDDE DAEDDDETEE
910 920 930 940 950
LGSDEDDIDE DGQEYLEILA KQAGEDGDDE DWEEDDAEET ALEGYSTIID
960 970 980 990 1000
DEDNPVDEYQ IFKAIFQTIQ NRNPVWYQAL THGLNEEQRK QLQDIATLAD
1010 1020 1030
QRRAAHESKM IEKHGGYKFS APVVPSSFNF GGPAPGMN
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A140LHD0 | A0A140LHD0_MOUSE | Importin-7 | Ipo7 | 70 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 160 | E → G in BAC35405 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 407 | V → VV in CAC17143 (PubMed:11528126).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278435 Genomic DNA Translation: CAC17143.1 AY316153 mRNA Translation: AAP79888.1 AK039534 mRNA Translation: BAC30376.1 AK053498 mRNA Translation: BAC35405.1 BC053524 mRNA Translation: AAH53524.1 BC064825 mRNA Translation: AAH64825.1 |
CCDSi | CCDS40085.1 |
RefSeqi | NP_852658.2, NM_181517.3 |
Genome annotation databases
Ensembli | ENSMUST00000084731; ENSMUSP00000081782; ENSMUSG00000066232 |
GeneIDi | 233726 |
KEGGi | mmu:233726 |
UCSCi | uc009jeu.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ278435 Genomic DNA Translation: CAC17143.1 AY316153 mRNA Translation: AAP79888.1 AK039534 mRNA Translation: BAC30376.1 AK053498 mRNA Translation: BAC35405.1 BC053524 mRNA Translation: AAH53524.1 BC064825 mRNA Translation: AAH64825.1 |
CCDSi | CCDS40085.1 |
RefSeqi | NP_852658.2, NM_181517.3 |
3D structure databases
SMRi | Q9EPL8 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 231437, 18 interactors |
IntActi | Q9EPL8, 6 interactors |
MINTi | Q9EPL8 |
STRINGi | 10090.ENSMUSP00000081782 |
Chemistry databases
ChEMBLi | CHEMBL2176785 |
PTM databases
iPTMneti | Q9EPL8 |
PhosphoSitePlusi | Q9EPL8 |
SwissPalmi | Q9EPL8 |
Proteomic databases
EPDi | Q9EPL8 |
jPOSTi | Q9EPL8 |
MaxQBi | Q9EPL8 |
PaxDbi | Q9EPL8 |
PeptideAtlasi | Q9EPL8 |
PRIDEi | Q9EPL8 |
Protocols and materials databases
Antibodypediai | 11566, 192 antibodies |
DNASUi | 233726 |
Genome annotation databases
Ensembli | ENSMUST00000084731; ENSMUSP00000081782; ENSMUSG00000066232 |
GeneIDi | 233726 |
KEGGi | mmu:233726 |
UCSCi | uc009jeu.1, mouse |
Organism-specific databases
CTDi | 10527 |
MGIi | MGI:2152414, Ipo7 |
Phylogenomic databases
eggNOGi | KOG1991, Eukaryota |
GeneTreei | ENSGT00940000154666 |
HOGENOMi | CLU_004196_1_1_1 |
InParanoidi | Q9EPL8 |
OMAi | VKPKSTW |
OrthoDBi | 159062at2759 |
PhylomeDBi | Q9EPL8 |
TreeFami | TF300634 |
Miscellaneous databases
BioGRID-ORCSi | 233726, 10 hits in 18 CRISPR screens |
ChiTaRSi | Ipo7, mouse |
PROi | PR:Q9EPL8 |
RNActi | Q9EPL8, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000066232, Expressed in parotid gland and 311 other tissues |
Genevisiblei | Q9EPL8, MM |
Family and domain databases
Gene3Di | 1.25.10.10, 1 hit |
InterProi | View protein in InterPro IPR011989, ARM-like IPR016024, ARM-type_fold IPR001494, Importin-beta_N IPR013713, XPO2_central |
Pfami | View protein in Pfam PF08506, Cse1, 1 hit PF03810, IBN_N, 1 hit |
SMARTi | View protein in SMART SM00913, IBN_N, 1 hit |
SUPFAMi | SSF48371, SSF48371, 1 hit |
PROSITEi | View protein in PROSITE PS50166, IMPORTIN_B_NT, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | IPO7_MOUSE | |
Accessioni | Q9EPL8Primary (citable) accession number: Q9EPL8 Secondary accession number(s): Q7TN09 Q8BYI0 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | March 15, 2004 |
Last sequence update: | March 15, 2004 | |
Last modified: | December 2, 2020 | |
This is version 159 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families