UniProtKB - Q9EPJ9 (ARFG1_MOUSE)
Protein
ADP-ribosylation factor GTPase-activating protein 1
Gene
Arfgap1
Organism
Mus musculus (Mouse)
Status
Functioni
GTPase-activating protein (GAP) for the ADP ribosylation factor 1 (ARF1). Involved in membrane trafficking and /or vesicle transport. Promotes hydrolysis of the ARF1-bound GTP and thus, is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles, a prerequisite for vesicle's fusion with target compartment. Probably regulates ARF1-mediated transport via its interaction with the KDELR proteins and TMED2. Overexpression induces the redistribution of the entire Golgi complex to the endoplasmic reticulum, as when ARF1 is deactivated. Its activity is stimulated by phosphoinosides and inhibited by phosphatidylcholine (By similarity).By similarity
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 22 – 45 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
GO - Molecular functioni
- GTPase activator activity Source: MGI
- metal ion binding Source: UniProtKB-KW
GO - Biological processi
- protein transport Source: UniProtKB-KW
- regulation of ARF protein signal transduction Source: GO_Central
- regulation of endocytosis Source: MGI
- vesicle-mediated transport Source: UniProtKB-KW
Keywordsi
Molecular function | GTPase activation |
Biological process | ER-Golgi transport, Protein transport, Transport |
Ligand | Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-MMU-6807878 COPI-mediated anterograde transport R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-MMU-8856828 Clathrin-mediated endocytosis |
Names & Taxonomyi
Protein namesi | Recommended name: ADP-ribosylation factor GTPase-activating protein 1Short name: ARF GAP 1 Alternative name(s): ADP-ribosylation factor 1 GTPase-activating protein Short name: ARF1 GAP ARF1-directed GTPase-activating protein |
Gene namesi | Name:Arfgap1 Synonyms:Arf1gap |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2183559 Arfgap1 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Other locations
- Cytoplasm By similarity
Note: Associates with the Golgi complex.By similarity
Golgi apparatus
- Golgi membrane Source: MGI
Other locations
- postsynaptic density Source: MGI
- synapse Source: MGI
Keywords - Cellular componenti
Cytoplasm, Golgi apparatusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000074191 | 1 – 414 | ADP-ribosylation factor GTPase-activating protein 1Add BLAST | 414 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 135 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 150 | PhosphoserineBy similarity | 1 | |
Modified residuei | 189 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 231 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 246 | PhosphoserineBy similarity | 1 | |
Modified residuei | 342 | PhosphoserineCombined sources | 1 | |
Modified residuei | 345 | PhosphoserineCombined sources | 1 | |
Modified residuei | 347 | PhosphoserineCombined sources | 1 | |
Modified residuei | 349 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 360 | PhosphoserineCombined sources | 1 | |
Modified residuei | 362 | PhosphoserineCombined sources | 1 | |
Modified residuei | 379 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
EPDi | Q9EPJ9 |
jPOSTi | Q9EPJ9 |
PaxDbi | Q9EPJ9 |
PeptideAtlasi | Q9EPJ9 |
PRIDEi | Q9EPJ9 |
PTM databases
iPTMneti | Q9EPJ9 |
PhosphoSitePlusi | Q9EPJ9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027575 Expressed in 281 organ(s), highest expression level in humerus cartilage element |
ExpressionAtlasi | Q9EPJ9 baseline and differential |
Genevisiblei | Q9EPJ9 MM |
Interactioni
Subunit structurei
Interacts with ARF1.
Interacts with the COPI coat proteins, KDELR1 and TMED2. It is probably a component of the COPI coat protein complex. The interaction with TMED2 inhibits the GAP activity (By similarity).
By similarityBinary interactionsi
With | Entry | #Exp. | IntAct | Notes |
---|---|---|---|---|
Q5S006 | 3 | EBI-6288020,EBI-2693710 |
Protein-protein interaction databases
BioGridi | 230805, 2 interactors |
IntActi | Q9EPJ9, 1 interactor |
STRINGi | 10090.ENSMUSP00000029092 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 7 – 124 | Arf-GAPPROSITE-ProRule annotationAdd BLAST | 118 |
Domaini
The region downstream of Arf-GAP domain is essential to GAP activity in vivo. This region may be required for its targeting to Golgi membranes (By similarity).By similarity
Zinc finger
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Zinc fingeri | 22 – 45 | C4-typePROSITE-ProRule annotationAdd BLAST | 24 |
Keywords - Domaini
Zinc-fingerPhylogenomic databases
eggNOGi | KOG0704 Eukaryota COG5347 LUCA |
GeneTreei | ENSGT00890000139515 |
HOGENOMi | HOG000008494 |
InParanoidi | Q9EPJ9 |
KOi | K12492 |
OMAi | VCCDCNA |
OrthoDBi | 1155557at2759 |
PhylomeDBi | Q9EPJ9 |
TreeFami | TF105931 |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412 ArfGap, 1 hit |
PRINTSi | PR00405 REVINTRACTNG |
SMARTi | View protein in SMART SM00105 ArfGap, 1 hit |
SUPFAMi | SSF57863 SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115 ARFGAP, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9EPJ9-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MASPRTRKVL KEVRAQDENN VCFECGAFNP QWVSVTYGIW ICLECSGRHR
60 70 80 90 100
GLGVHLSFVR SVTMDKWKDI ELEKMKAGGN AKFREFLETQ DDYEPSWSLQ
110 120 130 140 150
DKYSSRAAAL FRDKVATLAE GKEWSLESSP AQNWTPPQPK TLQFTAHRAS
160 170 180 190 200
GQPQSAAASG DKAFEDWLND DLGSYQGAQE NRYVGFGNTV PPQKREDDFL
210 220 230 240 250
NNAMSSLYSG WSSFTTGASK FASAAKEGAT KFGSQASQKA SELGHSLNEN
260 270 280 290 300
VLKPAQEKVK EGRIFDDVSS GVSQLASKVQ GVGSKGWRDV TTFFSGKAED
310 320 330 340 350
SSDRPLEGHS YQNSSGDNSQ NSNIDQSFWE TFGSAEPPKA KSPSSDSWTC
360 370 380 390 400
ADASTGRRSS DSWDVWGSGS ASNNKNSNSD GWESWEGASG EGRAKATKKA
410
APSTADEGWD NQNW
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketV9GXM1 | V9GXM1_MOUSE | ADP-ribosylation factor GTPase-acti... | Arfgap1 | 424 | Annotation score: | ||
Q3TGS9 | Q3TGS9_MOUSE | ADP-ribosylation factor GTPase-acti... | Arfgap1 | 394 | Annotation score: | ||
V9GWV1 | V9GWV1_MOUSE | ADP-ribosylation factor GTPase-acti... | Arfgap1 | 402 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 112 | R → K in CAC18721 (Ref. 1) Curated | 1 | |
Sequence conflicti | 188 | N → K in CAC18721 (Ref. 1) Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_011303 | 259 – 280 | Missing in isoform 2. 1 PublicationAdd BLAST | 22 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401461 mRNA Translation: CAC18721.1 AK030295 mRNA Translation: BAC26883.1 AK030520 mRNA Translation: BAC27002.1 AK145879 mRNA Translation: BAE26720.1 AK168007 mRNA Translation: BAE39994.1 BX649560 Genomic DNA No translation available. |
CCDSi | CCDS17190.1 [Q9EPJ9-1] CCDS50843.1 [Q9EPJ9-2] |
RefSeqi | NP_001171177.1, NM_001177706.1 [Q9EPJ9-2] NP_001171178.1, NM_001177707.1 NP_001171179.1, NM_001177708.1 NP_001171180.1, NM_001177709.1 [Q9EPJ9-2] NP_665703.2, NM_145760.3 [Q9EPJ9-1] XP_017173503.1, XM_017318014.1 [Q9EPJ9-1] |
Genome annotation databases
Ensembli | ENSMUST00000029092; ENSMUSP00000029092; ENSMUSG00000027575 [Q9EPJ9-1] ENSMUST00000108859; ENSMUSP00000104487; ENSMUSG00000027575 [Q9EPJ9-2] ENSMUST00000108860; ENSMUSP00000104488; ENSMUSG00000027575 [Q9EPJ9-2] |
GeneIDi | 228998 |
KEGGi | mmu:228998 |
UCSCi | uc008okj.2 mouse [Q9EPJ9-1] uc008okm.2 mouse [Q9EPJ9-2] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ401461 mRNA Translation: CAC18721.1 AK030295 mRNA Translation: BAC26883.1 AK030520 mRNA Translation: BAC27002.1 AK145879 mRNA Translation: BAE26720.1 AK168007 mRNA Translation: BAE39994.1 BX649560 Genomic DNA No translation available. |
CCDSi | CCDS17190.1 [Q9EPJ9-1] CCDS50843.1 [Q9EPJ9-2] |
RefSeqi | NP_001171177.1, NM_001177706.1 [Q9EPJ9-2] NP_001171178.1, NM_001177707.1 NP_001171179.1, NM_001177708.1 NP_001171180.1, NM_001177709.1 [Q9EPJ9-2] NP_665703.2, NM_145760.3 [Q9EPJ9-1] XP_017173503.1, XM_017318014.1 [Q9EPJ9-1] |
3D structure databases
SMRi | Q9EPJ9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGridi | 230805, 2 interactors |
IntActi | Q9EPJ9, 1 interactor |
STRINGi | 10090.ENSMUSP00000029092 |
PTM databases
iPTMneti | Q9EPJ9 |
PhosphoSitePlusi | Q9EPJ9 |
Proteomic databases
EPDi | Q9EPJ9 |
jPOSTi | Q9EPJ9 |
PaxDbi | Q9EPJ9 |
PeptideAtlasi | Q9EPJ9 |
PRIDEi | Q9EPJ9 |
Genome annotation databases
Ensembli | ENSMUST00000029092; ENSMUSP00000029092; ENSMUSG00000027575 [Q9EPJ9-1] ENSMUST00000108859; ENSMUSP00000104487; ENSMUSG00000027575 [Q9EPJ9-2] ENSMUST00000108860; ENSMUSP00000104488; ENSMUSG00000027575 [Q9EPJ9-2] |
GeneIDi | 228998 |
KEGGi | mmu:228998 |
UCSCi | uc008okj.2 mouse [Q9EPJ9-1] uc008okm.2 mouse [Q9EPJ9-2] |
Organism-specific databases
CTDi | 55738 |
MGIi | MGI:2183559 Arfgap1 |
Phylogenomic databases
eggNOGi | KOG0704 Eukaryota COG5347 LUCA |
GeneTreei | ENSGT00890000139515 |
HOGENOMi | HOG000008494 |
InParanoidi | Q9EPJ9 |
KOi | K12492 |
OMAi | VCCDCNA |
OrthoDBi | 1155557at2759 |
PhylomeDBi | Q9EPJ9 |
TreeFami | TF105931 |
Enzyme and pathway databases
Reactomei | R-MMU-6807878 COPI-mediated anterograde transport R-MMU-6811434 COPI-dependent Golgi-to-ER retrograde traffic R-MMU-8856828 Clathrin-mediated endocytosis |
Miscellaneous databases
ChiTaRSi | Arfgap1 mouse |
PROi | PR:Q9EPJ9 |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027575 Expressed in 281 organ(s), highest expression level in humerus cartilage element |
ExpressionAtlasi | Q9EPJ9 baseline and differential |
Genevisiblei | Q9EPJ9 MM |
Family and domain databases
Gene3Di | 3.30.40.160, 1 hit |
InterProi | View protein in InterPro IPR037278 ARFGAP/RecO IPR001164 ArfGAP_dom IPR038508 ArfGAP_dom_sf |
Pfami | View protein in Pfam PF01412 ArfGap, 1 hit |
PRINTSi | PR00405 REVINTRACTNG |
SMARTi | View protein in SMART SM00105 ArfGap, 1 hit |
SUPFAMi | SSF57863 SSF57863, 1 hit |
PROSITEi | View protein in PROSITE PS50115 ARFGAP, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ARFG1_MOUSE | |
Accessioni | Q9EPJ9Primary (citable) accession number: Q9EPJ9 Secondary accession number(s): A8WIR9 Q8BMQ7 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | January 27, 2003 |
Last sequence update: | August 16, 2004 | |
Last modified: | October 16, 2019 | |
This is version 144 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Complete proteome, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot