UniProtKB - Q9EPE9 (AT131_MOUSE)
Endoplasmic reticulum transmembrane helix translocase
Atp13a1
Functioni
Endoplasmic reticulum translocase required to remove mitochondrial transmembrane proteins mistargeted to the endoplasmic reticulum. Acts as a dislocase that mediates the ATP-dependent extraction of mislocalized mitochondrial transmembrane proteins from the endoplasmic reticulum membrane. Specifically binds mitochondrial tail-anchored transmembrane proteins: has an atypically large substrate-binding pocket that recognizes and binds moderately hydrophobic transmembranes with short hydrophilic lumenal domains.
By similarityCatalytic activityi
- [protein]-with a C-terminal TM segment(out) + ATP + H2O = [protein]-with a C-terminal TM segment(in) + ADP + H+ + phosphateBy similarity
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 530 | 4-aspartylphosphate intermediate1 Publication | 1 | |
Metal bindingi | 530 | MagnesiumBy similarity | 1 | |
Metal bindingi | 532 | MagnesiumBy similarity | 1 | |
Binding sitei | 623 | ATPBy similarity | 1 | |
Binding sitei | 681 | ATPBy similarity | 1 | |
Binding sitei | 746 | ATPBy similarity | 1 | |
Metal bindingi | 861 | MagnesiumBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 530 – 532 | ATPBy similarity | 3 | |
Nucleotide bindingi | 861 – 865 | ATPBy similarity | 5 |
GO - Molecular functioni
- ATPase-coupled cation transmembrane transporter activity Source: InterPro
- ATP binding Source: UniProtKB-KW
- ATP hydrolysis activity Source: InterPro
- metal ion binding Source: UniProtKB-KW
- transmembrane protein dislocase activity Source: UniProtKB
GO - Biological processi
- cellular calcium ion homeostasis Source: GO_Central
- extraction of mislocalized protein from ER membrane Source: UniProtKB
- protein transport Source: UniProtKB-KW
Keywordsi
Molecular function | Translocase |
Biological process | Protein transport, Transport |
Ligand | ATP-binding, Magnesium, Metal-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Names & Taxonomyi
Protein namesi | Recommended name: Endoplasmic reticulum transmembrane helix translocaseCurated (EC:7.4.2.-By similarity)Alternative name(s): Endoplasmic reticulum P5A-ATPaseBy similarity |
Gene namesi | |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2180801, Atp13a1 |
VEuPathDBi | HostDB:ENSMUSG00000031862 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane 1 Publication; Multi-pass membrane protein By similarity
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 63 | CytoplasmicSequence analysisAdd BLAST | 63 | |
Transmembranei | 64 – 84 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 85 – 92 | ExtracellularSequence analysis | 8 | |
Transmembranei | 93 – 113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 114 – 240 | CytoplasmicSequence analysisAdd BLAST | 127 | |
Transmembranei | 241 – 261 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 262 – 440 | ExtracellularSequence analysisAdd BLAST | 179 | |
Transmembranei | 441 – 461 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 462 – 985 | CytoplasmicSequence analysisAdd BLAST | 524 | |
Transmembranei | 986 – 1006 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1007 | ExtracellularSequence analysis | 1 | |
Transmembranei | 1008 – 1028 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1029 – 1047 | CytoplasmicSequence analysisAdd BLAST | 19 | |
Transmembranei | 1048 – 1068 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1069 – 1092 | ExtracellularSequence analysisAdd BLAST | 24 | |
Transmembranei | 1093 – 1113 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1114 – 1128 | CytoplasmicSequence analysisAdd BLAST | 15 | |
Transmembranei | 1129 – 1149 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1150 – 1162 | ExtracellularSequence analysisAdd BLAST | 13 | |
Transmembranei | 1163 – 1183 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 1184 – 1200 | CytoplasmicSequence analysisAdd BLAST | 17 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 530 | D → N: Loss of ATPase activity. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000046422 | 1 – 1200 | Endoplasmic reticulum transmembrane helix translocaseAdd BLAST | 1200 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 284 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 417 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Modified residuei | 896 | PhosphoserineCombined sources | 1 | |
Modified residuei | 902 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Glycoprotein, PhosphoproteinProteomic databases
EPDi | Q9EPE9 |
jPOSTi | Q9EPE9 |
MaxQBi | Q9EPE9 |
PaxDbi | Q9EPE9 |
PeptideAtlasi | Q9EPE9 |
PRIDEi | Q9EPE9 |
ProteomicsDBi | 277082 |
PTM databases
GlyGeni | Q9EPE9, 2 sites |
iPTMneti | Q9EPE9 |
PhosphoSitePlusi | Q9EPE9 |
SwissPalmi | Q9EPE9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000031862, Expressed in pancreas and 308 other tissues |
Genevisiblei | Q9EPE9, MM |
Interactioni
Protein-protein interaction databases
BioGRIDi | 228421, 5 interactors |
IntActi | Q9EPE9, 2 interactors |
STRINGi | 10090.ENSMUSP00000034326 |
Miscellaneous databases
RNActi | Q9EPE9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 196 – 227 | A-domain; part 1By similarityAdd BLAST | 32 | |
Regioni | 292 – 432 | A-domain; part 2By similarityAdd BLAST | 141 | |
Regioni | 509 – 538 | P-domain; part 1By similarityAdd BLAST | 30 | |
Regioni | 540 – 721 | N-domainBy similarityAdd BLAST | 182 | |
Regioni | 724 – 882 | P-domain; part 2By similarityAdd BLAST | 159 | |
Regioni | 883 – 945 | Arm-likeBy similarityAdd BLAST | 63 | |
Regioni | 886 – 928 | DisorderedSequence analysisAdd BLAST | 43 | |
Regioni | 946 – 961 | P-domain; part 3By similarityAdd BLAST | 16 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 900 – 916 | Polar residuesSequence analysisAdd BLAST | 17 |
Domaini
Sequence similaritiesi
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG0209, Eukaryota |
GeneTreei | ENSGT00550000075064 |
HOGENOMi | CLU_001828_4_1_1 |
InParanoidi | Q9EPE9 |
OMAi | RFAPKQK |
OrthoDBi | 172453at2759 |
PhylomeDBi | Q9EPE9 |
TreeFami | TF300725 |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006544, P-type_TPase_V IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01494, ATPase_P-type, 2 hits TIGR01657, P-ATPase-V, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MAVVGNAVPC GARPGGARDN GSPQPGSRLR PGLAAGPALI ANGDELVAAV
60 70 80 90 100
WPYRRLALLR RLTVLPFAGL LYPAWLGAAA SGCWGWGSSW TQIPEAALLA
110 120 130 140 150
LATICLAHAL TVLSGHWSVH AHCALTCTPE YDPNKVTFVK VVPTPNNGST
160 170 180 190 200
ELVALHRDKG EDGLEVLSFE FQKIKYSYDA LEKKQFLPVA FPVGNAFSYY
210 220 230 240 250
QSNRGFQEDS EIRAAEKKFG SNKAEMVVPD FSELFKERAT APFFVFQVFC
260 270 280 290 300
VGLWCLDEYW YYSVFTLSML VAFEASLVQQ QMRNMSEIRK MGNKPHMIQV
310 320 330 340 350
YRSRKWRPVA SDDIVPGDIV SIGRSPQENL VPCDVLLLRG RCIVDEAMLT
360 370 380 390 400
GESVPQMKEP IEDLSPDRVL DLQADARLHV IFGGTKVVQH IPPQKATSGL
410 420 430 440 450
KPVDNGCVAF VLRTGFNTSQ GRLLRTILFG VKRVTANNLE TFIFILFLLV
460 470 480 490 500
FAIAAAAYVW VEGTKDPSRN RYKLFLECTL ILTSVVPPEL PIELSLAVNT
510 520 530 540 550
SLIALAKLYM YCTEPFRIPF AGKVEVCCFD KTGTLTSDSL VVRGVAGLRD
560 570 580 590 600
GKEVTPVSSI PIETHRALAS CHSLMQLDDG TLVGDPLEKA MLTAVDWTLT
610 620 630 640 650
KDEKVFPRSI KTQGLKIHQR FHFASALKRM SVLASYEKLG STDLCYIAAV
660 670 680 690 700
KGAPETLHSM FSQCPPDYHH IHTEISREGA RVLALGYKEL GHLTHQQARE
710 720 730 740 750
IKREALECSL KFVGFIVVSC PLKADSKAVI REIQNASHRV VMITGDNPLT
760 770 780 790 800
ACHVAQELHF IDKAHTLILH PPSEKGQPCE WRSIDSSIVL PLTLGSPKAL
810 820 830 840 850
ALEHALCLTG DGLAHLQAVD PQQLLCLIPH VQVFARVAPK QKEFVITSLK
860 870 880 890 900
ELGYVTLMCG DGTNDVGALK HADVGVALLA NAPERVVERR RRPRDSPVLS
910 920 930 940 950
NSGPRVSRST KQKSALLSPE EPPASHRDRL SQVLRDLEEE STPIVKLGDA
960 970 980 990 1000
SIAAPFTSKL SSIQCICHVI KQGRCTLVTT LQMFKILALN ALILAYSQSV
1010 1020 1030 1040 1050
LYLEGVKFSD FQATLQGLLL AGCFLFISRS KPLKTLSRER PLPNIFNLYT
1060 1070 1080 1090 1100
ILTVMLQFSV HFLSLVYLYR EAQARSPEKQ EQFVDLYKEF EPSLVNSTVY
1110 1120 1130 1140 1150
IMAMAMQMAT FAINYKGPPF MESLPENKPL VWSLAVSLLA IIGLLLGSSP
1160 1170 1180 1190 1200
DFNSQFGLVD IPVEFKLVIG QVLALDFCLA LLADRVLQFF LGTPKLRVPS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A571BD32 | A0A571BD32_MOUSE | Endoplasmic reticulum transmembrane... | Atp13a1 | 69 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 188 | P → S in BAB20095 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035381 mRNA Translation: BAB20095.1 CH466569 Genomic DNA Translation: EDL28752.1 BC138721 mRNA Translation: AAI38722.1 BC138722 mRNA Translation: AAI38723.1 |
CCDSi | CCDS22349.1 |
RefSeqi | NP_573487.2, NM_133224.2 |
Genome annotation databases
Ensembli | ENSMUST00000034326; ENSMUSP00000034326; ENSMUSG00000031862 |
GeneIDi | 170759 |
KEGGi | mmu:170759 |
UCSCi | uc009lxr.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB035381 mRNA Translation: BAB20095.1 CH466569 Genomic DNA Translation: EDL28752.1 BC138721 mRNA Translation: AAI38722.1 BC138722 mRNA Translation: AAI38723.1 |
CCDSi | CCDS22349.1 |
RefSeqi | NP_573487.2, NM_133224.2 |
3D structure databases
AlphaFoldDBi | Q9EPE9 |
SMRi | Q9EPE9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 228421, 5 interactors |
IntActi | Q9EPE9, 2 interactors |
STRINGi | 10090.ENSMUSP00000034326 |
PTM databases
GlyGeni | Q9EPE9, 2 sites |
iPTMneti | Q9EPE9 |
PhosphoSitePlusi | Q9EPE9 |
SwissPalmi | Q9EPE9 |
Proteomic databases
EPDi | Q9EPE9 |
jPOSTi | Q9EPE9 |
MaxQBi | Q9EPE9 |
PaxDbi | Q9EPE9 |
PeptideAtlasi | Q9EPE9 |
PRIDEi | Q9EPE9 |
ProteomicsDBi | 277082 |
Protocols and materials databases
Antibodypediai | 28529, 83 antibodies from 27 providers |
DNASUi | 170759 |
Genome annotation databases
Ensembli | ENSMUST00000034326; ENSMUSP00000034326; ENSMUSG00000031862 |
GeneIDi | 170759 |
KEGGi | mmu:170759 |
UCSCi | uc009lxr.2, mouse |
Organism-specific databases
CTDi | 57130 |
MGIi | MGI:2180801, Atp13a1 |
VEuPathDBi | HostDB:ENSMUSG00000031862 |
Phylogenomic databases
eggNOGi | KOG0209, Eukaryota |
GeneTreei | ENSGT00550000075064 |
HOGENOMi | CLU_001828_4_1_1 |
InParanoidi | Q9EPE9 |
OMAi | RFAPKQK |
OrthoDBi | 172453at2759 |
PhylomeDBi | Q9EPE9 |
TreeFami | TF300725 |
Enzyme and pathway databases
Reactomei | R-MMU-936837, Ion transport by P-type ATPases |
Miscellaneous databases
BioGRID-ORCSi | 170759, 11 hits in 72 CRISPR screens |
ChiTaRSi | Atp13a1, mouse |
PROi | PR:Q9EPE9 |
RNActi | Q9EPE9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000031862, Expressed in pancreas and 308 other tissues |
Genevisiblei | Q9EPE9, MM |
Family and domain databases
Gene3Di | 3.40.1110.10, 1 hit 3.40.50.1000, 1 hit |
InterProi | View protein in InterPro IPR023299, ATPase_P-typ_cyto_dom_N IPR018303, ATPase_P-typ_P_site IPR023298, ATPase_P-typ_TM_dom_sf IPR008250, ATPase_P-typ_transduc_dom_A_sf IPR036412, HAD-like_sf IPR023214, HAD_sf IPR006544, P-type_TPase_V IPR001757, P_typ_ATPase IPR044492, P_typ_ATPase_HD_dom |
SFLDi | SFLDF00027, p-type_atpase, 1 hit |
SUPFAMi | SSF56784, SSF56784, 1 hit SSF81653, SSF81653, 1 hit SSF81660, SSF81660, 1 hit SSF81665, SSF81665, 1 hit |
TIGRFAMsi | TIGR01494, ATPase_P-type, 2 hits TIGR01657, P-ATPase-V, 1 hit |
PROSITEi | View protein in PROSITE PS00154, ATPASE_E1_E2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | AT131_MOUSE | |
Accessioni | Q9EPE9Primary (citable) accession number: Q9EPE9 Secondary accession number(s): B2RS54 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | May 25, 2022 | |
This is version 158 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families