UniProtKB - Q9EPB4 (ASC_MOUSE)
Apoptosis-associated speck-like protein containing a CARD
Pycard
Functioni
GO - Molecular functioni
- BMP receptor binding Source: MGI
- cysteine-type endopeptidase activity involved in apoptotic process Source: GO_Central
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- interleukin-6 receptor binding Source: MGI
- ion channel binding Source: MGI
- myosin I binding Source: MGI
- peptidase activator activity involved in apoptotic process Source: MGI
- protease binding Source: MGI
- protein dimerization activity Source: AgBase
- protein homodimerization activity Source: HGNC-UCL
- Pyrin domain binding Source: HGNC-UCL
- tropomyosin binding Source: MGI
GO - Biological processi
- activation of cysteine-type endopeptidase activity Source: CACAO
- activation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- activation of innate immune response Source: UniProtKB
- apoptotic process Source: MGI
- cellular response to interleukin-1 Source: MGI
- cellular response to lipopolysaccharide Source: MGI
- cellular response to tumor necrosis factor Source: MGI
- defense response to Gram-negative bacterium Source: MGI
- defense response to virus Source: MGI
- inflammatory response Source: MGI
- innate immune response Source: UniProtKB-KW
- interleukin-1 beta production Source: MGI
- intrinsic apoptotic signaling pathway by p53 class mediator Source: MGI
- intrinsic apoptotic signaling pathway in response to DNA damage by p53 class mediator Source: MGI
- macropinocytosis Source: UniProtKB
- myeloid dendritic cell activation Source: MGI
- myeloid dendritic cell activation involved in immune response Source: UniProtKB
- negative regulation of cytokine production involved in inflammatory response Source: UniProtKB
- negative regulation of I-kappaB kinase/NF-kappaB signaling Source: MGI
- negative regulation of interferon-beta production Source: MGI
- negative regulation of NF-kappaB transcription factor activity Source: MGI
- negative regulation of protein serine/threonine kinase activity Source: MGI
- positive regulation of actin filament polymerization Source: UniProtKB
- positive regulation of activated T cell proliferation Source: UniProtKB
- positive regulation of adaptive immune response Source: MGI
- positive regulation of antigen processing and presentation of peptide antigen via MHC class II Source: UniProtKB
- positive regulation of apoptotic process Source: MGI
- positive regulation of chemokine secretion Source: UniProtKB
- positive regulation of cysteine-type endopeptidase activity Source: MGI
- positive regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: HGNC-UCL
- positive regulation of defense response to virus by host Source: UniProtKB
- positive regulation of DNA-binding transcription factor activity Source: MGI
- positive regulation of ERK1 and ERK2 cascade Source: UniProtKB
- positive regulation of extrinsic apoptotic signaling pathway Source: MGI
- positive regulation of interferon-gamma production Source: UniProtKB
- positive regulation of interleukin-10 production Source: MGI
- positive regulation of interleukin-1 beta production Source: UniProtKB
- positive regulation of interleukin-6 production Source: UniProtKB
- positive regulation of interleukin-8 production Source: MGI
- positive regulation of JNK cascade Source: MGI
- positive regulation of NF-kappaB transcription factor activity Source: MGI
- positive regulation of phagocytosis Source: UniProtKB
- positive regulation of release of cytochrome c from mitochondria Source: MGI
- positive regulation of T cell activation Source: MGI
- positive regulation of T cell migration Source: UniProtKB
- positive regulation of tumor necrosis factor production Source: MGI
- protein homooligomerization Source: UniProtKB
- regulation of apoptotic process Source: MGI
- regulation of autophagy Source: MGI
- regulation of cysteine-type endopeptidase activity involved in apoptotic process Source: MGI
- regulation of GTPase activity Source: UniProtKB
- regulation of inflammatory response Source: MGI
- regulation of intrinsic apoptotic signaling pathway Source: MGI
- regulation of protein stability Source: UniProtKB
- regulation of tumor necrosis factor-mediated signaling pathway Source: MGI
- response to bacterium Source: MGI
- tumor necrosis factor-mediated signaling pathway Source: MGI
Keywordsi
Biological process | Apoptosis, Immunity, Inflammatory response, Innate immunity |
Enzyme and pathway databases
Reactomei | R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-6798695, Neutrophil degranulation |
Names & Taxonomyi
Protein namesi | Recommended name: Apoptosis-associated speck-like protein containing a CARDShort name: mASC Alternative name(s): PYD and CARD domain-containing protein |
Gene namesi | Name:Pycard Synonyms:Asc |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1931465, Pycard |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum By similarity
Nucleus
- Nucleus By similarity
Mitochondrion
- Mitochondrion By similarity
Other locations
- Cytoplasm 2 Publications
Note: Upstream of caspase activation, a redistribution from the cytoplasm to the aggregates occurs. These appear as hollow, perinuclear spherical, ball-like structures. Upon NLRP3 inflammasome activation redistributes to the perinuclear space localizing to endoplasmic reticulum and mitochondria. Localized primarily to the nucleus in resting monocytes/macrophages and rapidly redistributed to the cytoplasm upon pathogen infection (By similarity). Localized to large cytoplasmic aggregate appearing as a speck containing AIM2, PYCARD, CASP8 and bacterial DNA after infection with Francisella tularensis.By similarity
Cytosol
- AIM2 inflammasome complex Source: UniProtKB
- cytosol Source: MGI
- IkappaB kinase complex Source: Ensembl
- NLRP1 inflammasome complex Source: MGI
- NLRP3 inflammasome complex Source: UniProtKB
Endoplasmic reticulum
- endoplasmic reticulum Source: UniProtKB-SubCell
Extracellular region or secreted
- extracellular region Source: MGI
Golgi apparatus
- Golgi membrane Source: MGI
Mitochondrion
- mitochondrion Source: MGI
Nucleus
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: UniProtKB
Other locations
- cytoplasm Source: HGNC-UCL
- neuronal cell body Source: MGI
- protein-containing complex Source: MGI
Keywords - Cellular componenti
Cytoplasm, Endoplasmic reticulum, Mitochondrion, NucleusPathology & Biotechi
Disruption phenotypei
Keywords - Diseasei
Tumor suppressorPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000064693 | 1 – 193 | Apoptosis-associated speck-like protein containing a CARDAdd BLAST | 193 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 193 | PhosphoserineCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3636 |
EPDi | Q9EPB4 |
jPOSTi | Q9EPB4 |
MaxQBi | Q9EPB4 |
PaxDbi | Q9EPB4 |
PRIDEi | Q9EPB4 |
PTM databases
iPTMneti | Q9EPB4 |
PhosphoSitePlusi | Q9EPB4 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000030793, Expressed in small intestine and 247 other tissues |
Genevisiblei | Q9EPB4, MM |
Interactioni
Subunit structurei
Self-associates; enforced oligomerization induces apoptosis, NF-kappa-B regulation and interleukin-1 beta secretion. Homooligomers can form disk-like particles of approximately 12 nm diameter and approximately 1 nm height.
Component of several inflammasomes containing one pattern recognition receptor/sensor, such as NLRP1, NLRP2, NLRP3, AIM2, MEFV or NOD2, and probably NLRC4, NLRP12 or IFI16. Major component of the ASC pyroptosome, a 1-2 um supramolecular assembly (one per macrophage cell) which consists of oligomerized PYCARD dimers and CASP1.
Interacts with CASP1 (precursor form); the interaction induces activation of CASP1 leading to the processing of interleukin-1 beta; PYCARD competes with RIPK2 for binding to CASP1.
Interacts with NLRP3; the interaction requires the homooligomerization of NLRP3.
Interacts with NLRP2, NLRC4, MEFV, CARD16, AIM2, IFI16, NOD2, DDX58, RIPK2, PYDC1, PYDC2, NLRP10, CASP8, CHUK, IKBKB and BAX.
1 PublicationBinary interactionsi
Hide detailsGO - Molecular functioni
- BMP receptor binding Source: MGI
- enzyme binding Source: MGI
- identical protein binding Source: MGI
- interleukin-6 receptor binding Source: MGI
- ion channel binding Source: MGI
- myosin I binding Source: MGI
- protease binding Source: MGI
- protein dimerization activity Source: AgBase
- protein homodimerization activity Source: HGNC-UCL
- Pyrin domain binding Source: HGNC-UCL
- tropomyosin binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 211743, 3 interactors |
ComplexPortali | CPX-4241, NLRP3 inflammasome CPX-4243, AIM2 inflammasome CPX-4244, Pyrin inflammasome |
CORUMi | Q9EPB4 |
DIPi | DIP-27619N |
IntActi | Q9EPB4, 10 interactors |
MINTi | Q9EPB4 |
STRINGi | 10090.ENSMUSP00000033056 |
Miscellaneous databases
RNActi | Q9EPB4, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 1 – 91 | PyrinPROSITE-ProRule annotationAdd BLAST | 91 | |
Domaini | 105 – 193 | CARDPROSITE-ProRule annotationAdd BLAST | 89 |
Domaini
Phylogenomic databases
eggNOGi | ENOG502S3G5, Eukaryota |
GeneTreei | ENSGT00940000161873 |
HOGENOMi | CLU_113553_1_0_1 |
InParanoidi | Q9EPB4 |
OMAi | AWNLTCK |
OrthoDBi | 1494108at2759 |
PhylomeDBi | Q9EPB4 |
TreeFami | TF337882 |
Family and domain databases
CDDi | cd08330, CARD_ASC_NALP1, 1 hit |
InterProi | View protein in InterPro IPR001315, CARD IPR033516, CARD8/ASC/NALP1_CARD IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR002398, Pept_C14 |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
Pfami | View protein in Pfam PF00619, CARD, 1 hit PF02758, PYRIN, 1 hit |
SMARTi | View protein in SMART SM01289, PYRIN, 1 hit |
SUPFAMi | SSF47986, SSF47986, 2 hits |
PROSITEi | View protein in PROSITE PS50209, CARD, 1 hit PS50824, DAPIN, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MGRARDAILD ALENLSGDEL KKFKMKLLTV QLREGYGRIP RGALLQMDAI
60 70 80 90 100
DLTDKLVSYY LESYGLELTM TVLRDMGLQE LAEQLQTTKE ESGAVAAAAS
110 120 130 140 150
VPAQSTARTG HFVDQHRQAL IARVTEVDGV LDALHGSVLT EGQYQAVRAE
160 170 180 190
TTSQDKMRKL FSFVPSWNLT CKDSLLQALK EIHPYLVMDL EQS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RQ20 | A0A0U1RQ20_MOUSE | Apoptosis-associated speck-like pro... | Pycard | 151 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 159 | K → E in BAB31341 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032249 mRNA Translation: BAB16609.1 AF310104 mRNA Translation: AAG30287.1 AK009852 mRNA Translation: BAB26543.1 AK007742 mRNA Translation: BAB25229.1 AK018682 mRNA Translation: BAB31341.1 BC008252 mRNA Translation: AAH08252.1 |
CCDSi | CCDS21888.1 |
RefSeqi | NP_075747.3, NM_023258.4 |
Genome annotation databases
Ensembli | ENSMUST00000033056; ENSMUSP00000033056; ENSMUSG00000030793 |
GeneIDi | 66824 |
KEGGi | mmu:66824 |
UCSCi | uc009jxu.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AB032249 mRNA Translation: BAB16609.1 AF310104 mRNA Translation: AAG30287.1 AK009852 mRNA Translation: BAB26543.1 AK007742 mRNA Translation: BAB25229.1 AK018682 mRNA Translation: BAB31341.1 BC008252 mRNA Translation: AAH08252.1 |
CCDSi | CCDS21888.1 |
RefSeqi | NP_075747.3, NM_023258.4 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2N1F | Other | 4.00 | A/B/C/D/E/F/G/H/I/J/K/L/M/N/O | 2-90 | [»] | |
SMRi | Q9EPB4 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 211743, 3 interactors |
ComplexPortali | CPX-4241, NLRP3 inflammasome CPX-4243, AIM2 inflammasome CPX-4244, Pyrin inflammasome |
CORUMi | Q9EPB4 |
DIPi | DIP-27619N |
IntActi | Q9EPB4, 10 interactors |
MINTi | Q9EPB4 |
STRINGi | 10090.ENSMUSP00000033056 |
PTM databases
iPTMneti | Q9EPB4 |
PhosphoSitePlusi | Q9EPB4 |
Proteomic databases
CPTACi | non-CPTAC-3636 |
EPDi | Q9EPB4 |
jPOSTi | Q9EPB4 |
MaxQBi | Q9EPB4 |
PaxDbi | Q9EPB4 |
PRIDEi | Q9EPB4 |
Protocols and materials databases
Antibodypediai | 3375, 701 antibodies |
DNASUi | 66824 |
Genome annotation databases
Ensembli | ENSMUST00000033056; ENSMUSP00000033056; ENSMUSG00000030793 |
GeneIDi | 66824 |
KEGGi | mmu:66824 |
UCSCi | uc009jxu.2, mouse |
Organism-specific databases
CTDi | 29108 |
MGIi | MGI:1931465, Pycard |
Phylogenomic databases
eggNOGi | ENOG502S3G5, Eukaryota |
GeneTreei | ENSGT00940000161873 |
HOGENOMi | CLU_113553_1_0_1 |
InParanoidi | Q9EPB4 |
OMAi | AWNLTCK |
OrthoDBi | 1494108at2759 |
PhylomeDBi | Q9EPB4 |
TreeFami | TF337882 |
Enzyme and pathway databases
Reactomei | R-MMU-5660668, CLEC7A/inflammasome pathway R-MMU-6798695, Neutrophil degranulation |
Miscellaneous databases
BioGRID-ORCSi | 66824, 0 hits in 17 CRISPR screens |
PROi | PR:Q9EPB4 |
RNActi | Q9EPB4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000030793, Expressed in small intestine and 247 other tissues |
Genevisiblei | Q9EPB4, MM |
Family and domain databases
CDDi | cd08330, CARD_ASC_NALP1, 1 hit |
InterProi | View protein in InterPro IPR001315, CARD IPR033516, CARD8/ASC/NALP1_CARD IPR004020, DAPIN IPR011029, DEATH-like_dom_sf IPR002398, Pept_C14 |
PANTHERi | PTHR10454, PTHR10454, 1 hit |
Pfami | View protein in Pfam PF00619, CARD, 1 hit PF02758, PYRIN, 1 hit |
SMARTi | View protein in SMART SM01289, PYRIN, 1 hit |
SUPFAMi | SSF47986, SSF47986, 2 hits |
PROSITEi | View protein in PROSITE PS50209, CARD, 1 hit PS50824, DAPIN, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ASC_MOUSE | |
Accessioni | Q9EPB4Primary (citable) accession number: Q9EPB4 Secondary accession number(s): Q9D2W9 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 18, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 179 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references