Senp3

Functionⁱ

Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.2 Publications

Activity regulationⁱ

On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm.By similarity

Sites

Feature key	Position(s)	DescriptionActions	Length
Active siteⁱ	459	By similarity	1
Active siteⁱ	476	By similarity	1
Active siteⁱ	526	By similarity	1

GO - Molecular functionⁱ

thiol-dependent ubiquitin-specific protease activity
Source: MGI
Inferred from direct assayⁱ
- 11029585

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

protein desumoylation
Source: GO_Central
Inferred from biological aspect of ancestorⁱ
- 21873635
protein metabolic process
Source: MGI
Inferred from direct assayⁱ
- 11029585

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Hydrolase, Protease, Thiol protease
Biological process	Ubl conjugation pathway

Enzyme and pathway databases

BRENDAⁱ	3.4.22.B72 3474
Reactomeⁱ	R-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Protein family/group databases

MEROPS protease database More...MEROPSⁱ	C48.003

MEROPSⁱ

C48.003

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Sentrin-specific protease 3 (EC:3.4.22.-) Alternative name(s): SUMO-1-specific protease 3 Sentrin/SUMO-specific protease SENP3 Smt3-specific isopeptidase 1 Short name: Smt3ip1
Gene namesⁱ	Name:Senp3 Synonyms:Smt3ip, Smt3ip1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 11

Organism-specific databases

MGIⁱ	MGI:2158736 Senp3

Keywords - Cellular componentⁱ

Cytoplasm, Nucleus

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000101722	1 – 568	Sentrin-specific protease 3Add BLAST		568

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Modified residueⁱ	52	PhosphoserineBy similarity	1
Modified residueⁱ	71	PhosphoserineCombined sources	1
Modified residueⁱ	73	PhosphoserineCombined sources	1
Modified residueⁱ	163	PhosphoserineCombined sources	1
Modified residueⁱ	170	PhosphothreonineCombined sources	1
Modified residueⁱ	175	PhosphoserineCombined sources	1
Modified residueⁱ	182	PhosphoserineCombined sources	1
Modified residueⁱ	206	PhosphoserineCombined sources	1
Modified residueⁱ	226	PhosphoserineCombined sources	1

Keywords - PTMⁱ

Phosphoprotein

Proteomic databases

EPDⁱ	Q9EP97
MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9EP97
PaxDbⁱ	Q9EP97
PeptideAtlas More...PeptideAtlasⁱ	Q9EP97
PRIDEⁱ	Q9EP97

PTM databases

iPTMnetⁱ	Q9EP97
PhosphoSitePlusⁱ	Q9EP97

Expressionⁱ

Gene expression databases

Bgeeⁱ	ENSMUSG00000005204 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExⁱ	MM_SENP3
ExpressionAtlasⁱ	Q9EP97 baseline and differential
Genevisibleⁱ	Q9EP97 MM

Interactionⁱ

Subunit structureⁱ

Binds to SUMO1 and SUMO3. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8 (By similarity). Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9. Interacts with CCAR2 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridⁱ	219830, 4 interactors
Database of interacting proteins More...DIPⁱ	DIP-59204N
IntActⁱ	Q9EP97, 2 interactors
STRINGⁱ	10090.ENSMUSP00000005336

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9EP97
SMRⁱ	Q9EP97
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Region

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Regionⁱ	380 – 537	ProteaseAdd BLAST		158

Motif

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Motifⁱ	119 – 122	Nuclear localization signalSequence analysis		4
Motifⁱ	147 – 153	Nuclear localization signalSequence analysis		7

Compositional bias

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Compositional biasⁱ	13 – 66	Pro-richAdd BLAST		54
Compositional biasⁱ	70 – 86	Glu-richAdd BLAST		17

Sequence similaritiesⁱ

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGⁱ	KOG0778 Eukaryota COG5160 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00530000062941
HOGENOMⁱ	HOG000154287
HOVERGENⁱ	HBG059450
InParanoidⁱ	Q9EP97
KEGG Orthology (KO) More...KOⁱ	K08593
OMAⁱ	SLAHPKN
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G092N
PhylomeDBⁱ	Q9EP97
TreeFamⁱ	TF316289

Family and domain databases

InterProⁱ	View protein in InterPro IPR038765 Papain_like_cys_pep_sf IPR003653 Peptidase_C48_C IPR037945 SENP3
PANTHERⁱ	PTHR12606:SF16 PTHR12606:SF16, 1 hit
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF02902 Peptidase_C48, 1 hit
SUPFAMⁱ	SSF54001 SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50600 ULP_PROTEASE, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show all Align All

Q9EP97-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MKETIQGTGS WGPEPPGPGT TYSNPRRERL RWPLPPKPRL KSGGGFGPDP 
        60         70         80         90        100
GSGTTVPTRR LPAPRPSFDA SASEEEEEEE EEDEEEVAAW RLPPRWGQLG 
       110        120        130        140        150
ASQRSRALRP SHRKTCSQRR RRAMRAFQML LYSKSTSLTF HWKLWGRHRG 
       160        170        180        190        200
RRRNLAHPKN HLSPQEGGAT PQVPSPCCRF DSPRGLPPPR LGLLGALMAE 
       210        220        230        240        250
DGMRGSPPVP SGPPMEEDGL RWTPKSPLDP DSGLLSCTLP NGFGGLSGPE 
       260        270        280        290        300
GERSLAPPDA SILISNVCSI GDHVAQELFQ SSDLGIAEEA DRTGEKAGQH 
       310        320        330        340        350
SPLREEHVTC VQSILDEFLQ TYGSLIPLST DEVVEKLEDI FQQEFSTPSR 
       360        370        380        390        400
KSLVLQLIQS YQRMPGNAMV RGFRVSYKRH VLTMDDLGTL YGQNWLNDQV 
       410        420        430        440        450
MNMYGDLVMD TVPEKVHFFN SFFYDKLRTK GYDGVKRWTK NVDIFNKELL 
       460        470        480        490        500
LIPIHLEVHW SLISVDVRRR TITYFDSQRT LNRRCPKHIA KYLQAEAVKK 
       510        520        530        540        550
DRLDFHQGWK GYFKMNVARQ NNDSDCGAFV LQYCKHLALS QPFSFTQQDM 
       560 
PKLRRQIYKE LCHCKLTV

Length:568

Mass (Da):64,403

Last modified:March 1, 2001 - v1

Checksum:ⁱ655F1FAB1AB62EA8

GO

Computationally mapped potential isoform sequencesⁱ

There is 1 potential isoform mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation

	F6Z9L5	F6Z9L5_MOUSE		Sentrin-specific protease 3 Sentrin-specific protease 3	Senp3	169	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Sequence conflictⁱ	229	D → G in BAC32209 (PubMed:16141072).Curated		1
Sequence conflictⁱ	440	K → E in BAC40451 (PubMed:16141072).Curated		1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF194031 mRNA Translation: AAG28418.1 AY008764 mRNA Translation: AAG33253.1 AK045071 mRNA Translation: BAC32209.1 AK088608 mRNA Translation: BAC40451.1 AL603707 Genomic DNA No translation available. BC037014 mRNA Translation: AAH37014.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS24905.1
NCBI Reference Sequences More...RefSeqⁱ	NP_001157043.1, NM_001163571.1 NP_109627.3, NM_030702.4
UniGeneⁱ	Mm.284592 Mm.422171

Genome annotation databases

Ensemblⁱ	ENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204 ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204
GeneIDⁱ	80886
KEGGⁱ	mmu:80886
UCSC genome browser More...UCSCⁱ	uc007jrb.2 mouse

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9EP97	Sentrin-specific protease 3		HUMAN		574	UniRef90_Q9H4L4
Uncharacterized protein		RHIRO		580
Uncharacterized protein		RHIBE		580
SENP3 isoform 1		PANTR		574
Uncharacterized protein		PANPA		574
+121

Protein	Similar proteins		Species	Score	Length	Source
Q9EP97	Sentrin-specific protease 3		HUMAN		574	UniRef50_Q9H4L4
Uncharacterized protein		RHIRO		580
Uncharacterized protein		RHIBE		580
Uncharacterized protein		Cebus capucinus imitator		574
Uncharacterized protein		GORGO		574
+146

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF194031 mRNA Translation: AAG28418.1 AY008764 mRNA Translation: AAG33253.1 AK045071 mRNA Translation: BAC32209.1 AK088608 mRNA Translation: BAC40451.1 AL603707 Genomic DNA No translation available. BC037014 mRNA Translation: AAH37014.1
CCDSⁱ	CCDS24905.1
RefSeqⁱ	NP_001157043.1, NM_001163571.1 NP_109627.3, NM_030702.4
UniGeneⁱ	Mm.284592 Mm.422171

3D structure databases

ProteinModelPortalⁱ	Q9EP97
SMRⁱ	Q9EP97
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

BioGridⁱ	219830, 4 interactors
DIPⁱ	DIP-59204N
IntActⁱ	Q9EP97, 2 interactors
STRINGⁱ	10090.ENSMUSP00000005336

Protein family/group databases

MEROPSⁱ	C48.003

MEROPSⁱ

C48.003

PTM databases

iPTMnetⁱ	Q9EP97
PhosphoSitePlusⁱ	Q9EP97

Proteomic databases

EPDⁱ	Q9EP97
MaxQBⁱ	Q9EP97
PaxDbⁱ	Q9EP97
PeptideAtlasⁱ	Q9EP97
PRIDEⁱ	Q9EP97

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204 ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204
GeneIDⁱ	80886
KEGGⁱ	mmu:80886
UCSCⁱ	uc007jrb.2 mouse

Organism-specific databases

CTDⁱ	26168
MGIⁱ	MGI:2158736 Senp3

Phylogenomic databases

eggNOGⁱ	KOG0778 Eukaryota COG5160 LUCA
GeneTreeⁱ	ENSGT00530000062941
HOGENOMⁱ	HOG000154287
HOVERGENⁱ	HBG059450
InParanoidⁱ	Q9EP97
KOⁱ	K08593
OMAⁱ	SLAHPKN
OrthoDBⁱ	EOG091G092N
PhylomeDBⁱ	Q9EP97
TreeFamⁱ	TF316289

Enzyme and pathway databases

BRENDAⁱ	3.4.22.B72 3474
Reactomeⁱ	R-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

Protein Ontology More...PROⁱ	PR:Q9EP97
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000005204 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExⁱ	MM_SENP3
ExpressionAtlasⁱ	Q9EP97 baseline and differential
Genevisibleⁱ	Q9EP97 MM

Family and domain databases

InterProⁱ	View protein in InterPro IPR038765 Papain_like_cys_pep_sf IPR003653 Peptidase_C48_C IPR037945 SENP3
PANTHERⁱ	PTHR12606:SF16 PTHR12606:SF16, 1 hit
Pfamⁱ	View protein in Pfam PF02902 Peptidase_C48, 1 hit
SUPFAMⁱ	SSF54001 SSF54001, 1 hit
PROSITEⁱ	View protein in PROSITE PS50600 ULP_PROTEASE, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	SENP3_MOUSE
Accessionⁱ	Q9EP97Primary (citable) accession number: Q9EP97 Secondary accession number(s): Q5F2A5, Q8BRD5, Q8C2H5
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	November 28, 2002
	Last sequence update:	March 1, 2001
	Last modified:	November 7, 2018
	This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
Peptidase families
Classification of peptidase families and list of entries
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

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UniProtKB - Q9EP97 (SENP3_MOUSE)

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Sentrin-specific protease 3

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<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

Sites

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Protein family/group databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Nucleus

Other locations

Nucleus

Other locations

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Region

Motif

Compositional bias

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Phylogenomic databases

Family and domain databases

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Protein family/group databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ