UniProtKB - Q9EP97 (SENP3_MOUSE)
Sentrin-specific protease 3
Senp3
Functioni
Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates (PubMed:11029585).
Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability (By similarity).
Deconjugates SUMO2 and SUMO3 from CDCA8 (By similarity).
Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300 (By similarity).
Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin, NPM1 (By similarity).
Plays a role in the regulation of sumoylation status of ZNF148 (By similarity).
Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes (PubMed:22872859).
Deconjugates SUMO2 from KAT5 (By similarity).
By similarity2 PublicationsActivity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 459 | By similarity | 1 | |
Active sitei | 476 | By similarity | 1 | |
Active sitei | 526 | NucleophileBy similarity | 1 |
GO - Molecular functioni
- SUMO-specific isopeptidase activity Source: MGI
- thiol-dependent deubiquitinase Source: MGI
GO - Biological processi
- protein desumoylation Source: MGI
- protein metabolic process Source: MGI
Keywordsi
Molecular function | Hydrolase, Protease, Thiol protease |
Biological process | Ubl conjugation pathway |
Enzyme and pathway databases
BRENDAi | 3.4.22.B72, 3474 |
Reactomei | R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Protein family/group databases
MEROPSi | C48.003 |
Names & Taxonomyi
Protein namesi | Recommended name: Sentrin-specific protease 3 (EC:3.4.22.-)Alternative name(s): SUMO-1-specific protease 3 Sentrin/SUMO-specific protease SENP3 Smt3-specific isopeptidase 11 Publication Short name: Smt3ip11 Publication |
Gene namesi | Name:Senp3 Synonyms:Smt3ip, Smt3ip11 Publication |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:2158736, Senp3 |
VEuPathDBi | HostDB:ENSMUSG00000005204 |
Subcellular locationi
Nucleus
- nucleolus 1 Publication
- nucleoplasm 1 Publication
Cytoplasm and Cytosol
- Cytoplasm 1 Publication
Note: Redistributes between the nucleolus and the nucleoplasm in response to mild oxidative stress (By similarity). Mainly found in the nucleoplasm, with low levels detected in the cytoplasmic and chromatin fractions (PubMed:22872859).By similarity1 Publication
Nucleus
- MLL1 complex Source: UniProtKB
- nucleolus Source: MGI
- nucleoplasm Source: MGI
- nucleus Source: GO_Central
Other locations
- cytoplasm Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cytoplasm, NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000101722 | 1 – 568 | Sentrin-specific protease 3Add BLAST | 568 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 52 | PhosphoserineBy similarity | 1 | |
Modified residuei | 71 | PhosphoserineCombined sources | 1 | |
Modified residuei | 73 | PhosphoserineCombined sources | 1 | |
Modified residuei | 163 | PhosphoserineCombined sources | 1 | |
Modified residuei | 170 | PhosphothreonineCombined sources | 1 | |
Modified residuei | 175 | PhosphoserineCombined sources | 1 | |
Modified residuei | 182 | PhosphoserineCombined sources | 1 | |
Modified residuei | 206 | PhosphoserineCombined sources | 1 | |
Modified residuei | 226 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9EP97 |
jPOSTi | Q9EP97 |
MaxQBi | Q9EP97 |
PaxDbi | Q9EP97 |
PeptideAtlasi | Q9EP97 |
PRIDEi | Q9EP97 |
ProteomicsDBi | 256960 |
PTM databases
iPTMneti | Q9EP97 |
PhosphoSitePlusi | Q9EP97 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000005204, Expressed in cumulus cell and 316 other tissues |
ExpressionAtlasi | Q9EP97, baseline and differential |
Genevisiblei | Q9EP97, MM |
Interactioni
Subunit structurei
Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10 (By similarity).
Interacts with EP300, NPM1 and CDCA8 (By similarity).
Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148 (PubMed:22872859).
Interacts with NOL9 (PubMed:22872859).
Interacts with CCAR2 (By similarity).
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 219830, 6 interactors |
DIPi | DIP-59204N |
IntActi | Q9EP97, 2 interactors |
STRINGi | 10090.ENSMUSP00000005336 |
Miscellaneous databases
RNActi | Q9EP97, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 119 | DisorderedSequence analysisAdd BLAST | 119 | |
Regioni | 155 – 174 | DisorderedSequence analysisAdd BLAST | 20 | |
Regioni | 380 – 537 | ProteaseAdd BLAST | 158 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 119 – 122 | Nuclear localization signalSequence analysis | 4 | |
Motifi | 147 – 153 | Nuclear localization signalSequence analysis | 7 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 72 – 87 | Acidic residuesSequence analysisAdd BLAST | 16 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG0778, Eukaryota |
GeneTreei | ENSGT00940000156309 |
HOGENOMi | CLU_035238_0_0_1 |
InParanoidi | Q9EP97 |
OMAi | YCKYLAL |
OrthoDBi | 1480705at2759 |
PhylomeDBi | Q9EP97 |
TreeFami | TF316289 |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C IPR037945, SENP3 IPR045577, SENP3_5_cons_dom |
PANTHERi | PTHR12606:SF16, PTHR12606:SF16, 1 hit |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 1 hit PF19722, SENP3_5_N, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MKETIQGTGS WGPEPPGPGT TYSNPRRERL RWPLPPKPRL KSGGGFGPDP
60 70 80 90 100
GSGTTVPTRR LPAPRPSFDA SASEEEEEEE EEDEEEVAAW RLPPRWGQLG
110 120 130 140 150
ASQRSRALRP SHRKTCSQRR RRAMRAFQML LYSKSTSLTF HWKLWGRHRG
160 170 180 190 200
RRRNLAHPKN HLSPQEGGAT PQVPSPCCRF DSPRGLPPPR LGLLGALMAE
210 220 230 240 250
DGMRGSPPVP SGPPMEEDGL RWTPKSPLDP DSGLLSCTLP NGFGGLSGPE
260 270 280 290 300
GERSLAPPDA SILISNVCSI GDHVAQELFQ SSDLGIAEEA DRTGEKAGQH
310 320 330 340 350
SPLREEHVTC VQSILDEFLQ TYGSLIPLST DEVVEKLEDI FQQEFSTPSR
360 370 380 390 400
KSLVLQLIQS YQRMPGNAMV RGFRVSYKRH VLTMDDLGTL YGQNWLNDQV
410 420 430 440 450
MNMYGDLVMD TVPEKVHFFN SFFYDKLRTK GYDGVKRWTK NVDIFNKELL
460 470 480 490 500
LIPIHLEVHW SLISVDVRRR TITYFDSQRT LNRRCPKHIA KYLQAEAVKK
510 520 530 540 550
DRLDFHQGWK GYFKMNVARQ NNDSDCGAFV LQYCKHLALS QPFSFTQQDM
560
PKLRRQIYKE LCHCKLTV
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketF6Z9L5 | F6Z9L5_MOUSE | Sentrin-specific protease 3 | Senp3 | 169 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 229 | D → G in BAC32209 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 440 | K → E in BAC40451 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF194031 mRNA Translation: AAG28418.1 AY008764 mRNA Translation: AAG33253.1 AK045071 mRNA Translation: BAC32209.1 AK088608 mRNA Translation: BAC40451.1 AL603707 Genomic DNA No translation available. BC037014 mRNA Translation: AAH37014.1 |
CCDSi | CCDS24905.1 |
RefSeqi | NP_001157043.1, NM_001163571.1 NP_109627.3, NM_030702.4 |
Genome annotation databases
Ensembli | ENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204 ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204 |
GeneIDi | 80886 |
KEGGi | mmu:80886 |
UCSCi | uc007jrb.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF194031 mRNA Translation: AAG28418.1 AY008764 mRNA Translation: AAG33253.1 AK045071 mRNA Translation: BAC32209.1 AK088608 mRNA Translation: BAC40451.1 AL603707 Genomic DNA No translation available. BC037014 mRNA Translation: AAH37014.1 |
CCDSi | CCDS24905.1 |
RefSeqi | NP_001157043.1, NM_001163571.1 NP_109627.3, NM_030702.4 |
3D structure databases
SMRi | Q9EP97 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 219830, 6 interactors |
DIPi | DIP-59204N |
IntActi | Q9EP97, 2 interactors |
STRINGi | 10090.ENSMUSP00000005336 |
Protein family/group databases
MEROPSi | C48.003 |
PTM databases
iPTMneti | Q9EP97 |
PhosphoSitePlusi | Q9EP97 |
Proteomic databases
EPDi | Q9EP97 |
jPOSTi | Q9EP97 |
MaxQBi | Q9EP97 |
PaxDbi | Q9EP97 |
PeptideAtlasi | Q9EP97 |
PRIDEi | Q9EP97 |
ProteomicsDBi | 256960 |
Protocols and materials databases
DNASUi | 80886 |
Genome annotation databases
Ensembli | ENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204 ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204 |
GeneIDi | 80886 |
KEGGi | mmu:80886 |
UCSCi | uc007jrb.2, mouse |
Organism-specific databases
CTDi | 26168 |
MGIi | MGI:2158736, Senp3 |
VEuPathDBi | HostDB:ENSMUSG00000005204 |
Phylogenomic databases
eggNOGi | KOG0778, Eukaryota |
GeneTreei | ENSGT00940000156309 |
HOGENOMi | CLU_035238_0_0_1 |
InParanoidi | Q9EP97 |
OMAi | YCKYLAL |
OrthoDBi | 1480705at2759 |
PhylomeDBi | Q9EP97 |
TreeFami | TF316289 |
Enzyme and pathway databases
BRENDAi | 3.4.22.B72, 3474 |
Reactomei | R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol |
Miscellaneous databases
BioGRID-ORCSi | 80886, 4 hits in 65 CRISPR screens |
ChiTaRSi | Senp3, mouse |
PROi | PR:Q9EP97 |
RNActi | Q9EP97, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000005204, Expressed in cumulus cell and 316 other tissues |
ExpressionAtlasi | Q9EP97, baseline and differential |
Genevisiblei | Q9EP97, MM |
Family and domain databases
InterProi | View protein in InterPro IPR038765, Papain-like_cys_pep_sf IPR003653, Peptidase_C48_C IPR037945, SENP3 IPR045577, SENP3_5_cons_dom |
PANTHERi | PTHR12606:SF16, PTHR12606:SF16, 1 hit |
Pfami | View protein in Pfam PF02902, Peptidase_C48, 1 hit PF19722, SENP3_5_N, 1 hit |
SUPFAMi | SSF54001, SSF54001, 1 hit |
PROSITEi | View protein in PROSITE PS50600, ULP_PROTEASE, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | SENP3_MOUSE | |
Accessioni | Q9EP97Primary (citable) accession number: Q9EP97 Secondary accession number(s): Q5F2A5, Q8BRD5, Q8C2H5 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | November 28, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | February 23, 2022 | |
This is version 150 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- Peptidase families
Classification of peptidase families and list of entries - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families