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Protein

Sentrin-specific protease 3

Gene

Senp3

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Protease that releases SUMO2 and SUMO3 monomers from sumoylated substrates, but has only weak activity against SUMO1 conjugates. Deconjugates SUMO2 from MEF2D, which increases its transcriptional activation capability. Deconjugates SUMO2 and SUMO3 from CDCA8. Redox sensor that, when redistributed into nucleoplasm, can act as an effector to enhance HIF1A transcriptional activity by desumoylating EP300. Required for rRNA processing through deconjugation of SUMO2 and SUMO3 from nucleophosmin. Plays a role in the regulation of sumoylation status of ZNF148. Functions as a component of the Five Friends of Methylated CHTOP (5FMC) complex; the 5FMC complex is recruited to ZNF148 by methylated CHTOP, leading to desumoylation of ZNF148 and subsequent transactivation of ZNF148 target genes.2 Publications

Activity regulationi

On oxidative stress, SENP3 degradation is blocked by inhibition of its ubiquitination, which stabilizes it as it accumulates in the nucleoplasm.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei459By similarity1
Active sitei476By similarity1
Active sitei526By similarity1

GO - Molecular functioni

  • thiol-dependent ubiquitin-specific protease activity Source: MGI

GO - Biological processi

  • protein desumoylation Source: GO_Central
  • protein metabolic process Source: MGI

Keywordsi

Molecular functionHydrolase, Protease, Thiol protease
Biological processUbl conjugation pathway

Enzyme and pathway databases

BRENDAi3.4.22.B72 3474
ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Protein family/group databases

MEROPSiC48.003

Names & Taxonomyi

Protein namesi
Recommended name:
Sentrin-specific protease 3 (EC:3.4.22.-)
Alternative name(s):
SUMO-1-specific protease 3
Sentrin/SUMO-specific protease SENP3
Smt3-specific isopeptidase 1
Short name:
Smt3ip1
Gene namesi
Name:Senp3
Synonyms:Smt3ip, Smt3ip1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 11

Organism-specific databases

MGIiMGI:2158736 Senp3

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001017221 – 568Sentrin-specific protease 3Add BLAST568

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei52PhosphoserineBy similarity1
Modified residuei71PhosphoserineCombined sources1
Modified residuei73PhosphoserineCombined sources1
Modified residuei163PhosphoserineCombined sources1
Modified residuei170PhosphothreonineCombined sources1
Modified residuei175PhosphoserineCombined sources1
Modified residuei182PhosphoserineCombined sources1
Modified residuei206PhosphoserineCombined sources1
Modified residuei226PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ9EP97
MaxQBiQ9EP97
PaxDbiQ9EP97
PeptideAtlasiQ9EP97
PRIDEiQ9EP97

PTM databases

iPTMnetiQ9EP97
PhosphoSitePlusiQ9EP97

Expressioni

Gene expression databases

BgeeiENSMUSG00000005204 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExiMM_SENP3
ExpressionAtlasiQ9EP97 baseline and differential
GenevisibleiQ9EP97 MM

Interactioni

Subunit structurei

Binds to SUMO1 and SUMO3. Component of some MLL1/MLL complex, at least composed of the core components KMT2A/MLL1, ASH2L, HCFC1/HCF1, WDR5 and RBBP5, as well as the facultative components BAP18, CHD8, E2F6, HSP70, INO80C, KANSL1, LAS1L, MAX, MCRS1, MGA, MYST1/MOF, PELP1, PHF20, PRP31, RING2, RUVB1/TIP49A, RUVB2/TIP49B, SENP3, TAF1, TAF4, TAF6, TAF7, TAF9 and TEX10. Interacts with EP300, NPM1 and CDCA8 (By similarity). Component of the 5FMC complex, at least composed of PELP1, LAS1L, TEX10, WDR18 and SENP3; the complex interacts with methylated CHTOP and ZNF148. Interacts with NOL9. Interacts with CCAR2 (By similarity).By similarity1 Publication

Protein-protein interaction databases

BioGridi219830, 4 interactors
DIPiDIP-59204N
IntActiQ9EP97, 2 interactors
STRINGi10090.ENSMUSP00000005336

Structurei

3D structure databases

ProteinModelPortaliQ9EP97
SMRiQ9EP97
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni380 – 537ProteaseAdd BLAST158

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi119 – 122Nuclear localization signalSequence analysis4
Motifi147 – 153Nuclear localization signalSequence analysis7

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi13 – 66Pro-richAdd BLAST54
Compositional biasi70 – 86Glu-richAdd BLAST17

Sequence similaritiesi

Belongs to the peptidase C48 family.Curated

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154287
HOVERGENiHBG059450
InParanoidiQ9EP97
KOiK08593
OMAiSLAHPKN
OrthoDBiEOG091G092N
PhylomeDBiQ9EP97
TreeFamiTF316289

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
IPR037945 SENP3
PANTHERiPTHR12606:SF16 PTHR12606:SF16, 1 hit
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9EP97-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKETIQGTGS WGPEPPGPGT TYSNPRRERL RWPLPPKPRL KSGGGFGPDP
60 70 80 90 100
GSGTTVPTRR LPAPRPSFDA SASEEEEEEE EEDEEEVAAW RLPPRWGQLG
110 120 130 140 150
ASQRSRALRP SHRKTCSQRR RRAMRAFQML LYSKSTSLTF HWKLWGRHRG
160 170 180 190 200
RRRNLAHPKN HLSPQEGGAT PQVPSPCCRF DSPRGLPPPR LGLLGALMAE
210 220 230 240 250
DGMRGSPPVP SGPPMEEDGL RWTPKSPLDP DSGLLSCTLP NGFGGLSGPE
260 270 280 290 300
GERSLAPPDA SILISNVCSI GDHVAQELFQ SSDLGIAEEA DRTGEKAGQH
310 320 330 340 350
SPLREEHVTC VQSILDEFLQ TYGSLIPLST DEVVEKLEDI FQQEFSTPSR
360 370 380 390 400
KSLVLQLIQS YQRMPGNAMV RGFRVSYKRH VLTMDDLGTL YGQNWLNDQV
410 420 430 440 450
MNMYGDLVMD TVPEKVHFFN SFFYDKLRTK GYDGVKRWTK NVDIFNKELL
460 470 480 490 500
LIPIHLEVHW SLISVDVRRR TITYFDSQRT LNRRCPKHIA KYLQAEAVKK
510 520 530 540 550
DRLDFHQGWK GYFKMNVARQ NNDSDCGAFV LQYCKHLALS QPFSFTQQDM
560
PKLRRQIYKE LCHCKLTV
Length:568
Mass (Da):64,403
Last modified:March 1, 2001 - v1
Checksum:i655F1FAB1AB62EA8
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
F6Z9L5F6Z9L5_MOUSE
Sentrin-specific protease 3
Senp3
169Annotation score:

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti229D → G in BAC32209 (PubMed:16141072).Curated1
Sequence conflicti440K → E in BAC40451 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF194031 mRNA Translation: AAG28418.1
AY008764 mRNA Translation: AAG33253.1
AK045071 mRNA Translation: BAC32209.1
AK088608 mRNA Translation: BAC40451.1
AL603707 Genomic DNA No translation available.
BC037014 mRNA Translation: AAH37014.1
CCDSiCCDS24905.1
RefSeqiNP_001157043.1, NM_001163571.1
NP_109627.3, NM_030702.4
UniGeneiMm.284592
Mm.422171

Genome annotation databases

EnsembliENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204
ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204
GeneIDi80886
KEGGimmu:80886
UCSCiuc007jrb.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF194031 mRNA Translation: AAG28418.1
AY008764 mRNA Translation: AAG33253.1
AK045071 mRNA Translation: BAC32209.1
AK088608 mRNA Translation: BAC40451.1
AL603707 Genomic DNA No translation available.
BC037014 mRNA Translation: AAH37014.1
CCDSiCCDS24905.1
RefSeqiNP_001157043.1, NM_001163571.1
NP_109627.3, NM_030702.4
UniGeneiMm.284592
Mm.422171

3D structure databases

ProteinModelPortaliQ9EP97
SMRiQ9EP97
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi219830, 4 interactors
DIPiDIP-59204N
IntActiQ9EP97, 2 interactors
STRINGi10090.ENSMUSP00000005336

Protein family/group databases

MEROPSiC48.003

PTM databases

iPTMnetiQ9EP97
PhosphoSitePlusiQ9EP97

Proteomic databases

EPDiQ9EP97
MaxQBiQ9EP97
PaxDbiQ9EP97
PeptideAtlasiQ9EP97
PRIDEiQ9EP97

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000005336; ENSMUSP00000005336; ENSMUSG00000005204
ENSMUST00000066760; ENSMUSP00000066581; ENSMUSG00000005204
GeneIDi80886
KEGGimmu:80886
UCSCiuc007jrb.2 mouse

Organism-specific databases

CTDi26168
MGIiMGI:2158736 Senp3

Phylogenomic databases

eggNOGiKOG0778 Eukaryota
COG5160 LUCA
GeneTreeiENSGT00530000062941
HOGENOMiHOG000154287
HOVERGENiHBG059450
InParanoidiQ9EP97
KOiK08593
OMAiSLAHPKN
OrthoDBiEOG091G092N
PhylomeDBiQ9EP97
TreeFamiTF316289

Enzyme and pathway databases

BRENDAi3.4.22.B72 3474
ReactomeiR-MMU-6791226 Major pathway of rRNA processing in the nucleolus and cytosol

Miscellaneous databases

PROiPR:Q9EP97
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000005204 Expressed in 292 organ(s), highest expression level in cumulus cell
CleanExiMM_SENP3
ExpressionAtlasiQ9EP97 baseline and differential
GenevisibleiQ9EP97 MM

Family and domain databases

InterProiView protein in InterPro
IPR038765 Papain_like_cys_pep_sf
IPR003653 Peptidase_C48_C
IPR037945 SENP3
PANTHERiPTHR12606:SF16 PTHR12606:SF16, 1 hit
PfamiView protein in Pfam
PF02902 Peptidase_C48, 1 hit
SUPFAMiSSF54001 SSF54001, 1 hit
PROSITEiView protein in PROSITE
PS50600 ULP_PROTEASE, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiSENP3_MOUSE
AccessioniPrimary (citable) accession number: Q9EP97
Secondary accession number(s): Q5F2A5, Q8BRD5, Q8C2H5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: November 28, 2002
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 133 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Peptidase families
    Classification of peptidase families and list of entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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