UniProtKB - Q9EP80 (PICK1_RAT)
PRKCA-binding protein
Pick1
Functioni
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Metal bindingi | 44 | ZincCurated | 1 | |
Metal bindingi | 46 | ZincCurated | 1 |
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- Arp2/3 complex binding Source: UniProtKB
- cytoskeletal protein binding Source: RGD
- enzyme binding Source: RGD
- ephrin receptor binding Source: UniProtKB
- G protein-coupled glutamate receptor binding Source: UniProtKB
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- GTPase binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- ionotropic glutamate receptor binding Source: UniProtKB
- membrane curvature sensor activity Source: RGD
- metal ion binding Source: UniProtKB-KW
- phospholipid binding Source: GO_Central
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase C binding Source: UniProtKB
- receptor tyrosine kinase binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
- SNAP receptor activity Source: UniProtKB
GO - Biological processi
- cellular response to decreased oxygen levels Source: UniProtKB
- cellular response to glucose starvation Source: UniProtKB
- dendritic spine maintenance Source: UniProtKB
- dendritic spine organization Source: UniProtKB
- dopamine transport Source: UniProtKB
- glial cell development Source: UniProtKB
- intracellular protein transport Source: GO_Central
- long-term synaptic depression Source: UniProtKB
- monoamine transport Source: UniProtKB
- negative regulation of Arp2/3 complex-mediated actin nucleation Source: UniProtKB
- negative regulation of cell death Source: ParkinsonsUK-UCL
- negative regulation of gene expression Source: ParkinsonsUK-UCL
- positive regulation of receptor internalization Source: UniProtKB
- protein kinase C-activating G protein-coupled receptor signaling pathway Source: UniProtKB
- protein phosphorylation Source: UniProtKB
- protein targeting Source: RGD
- receptor clustering Source: UniProtKB
- regulation of Arp2/3 complex-mediated actin nucleation Source: GO_Central
- regulation of insulin secretion Source: RGD
- regulation of postsynaptic neurotransmitter receptor internalization Source: SynGO
Keywordsi
Molecular function | Actin-binding |
Ligand | Calcium, Metal-binding, Zinc |
Enzyme and pathway databases
Reactomei | R-RNO-416993, Trafficking of GluR2-containing AMPA receptors |
Names & Taxonomyi
Protein namesi | Recommended name: PRKCA-binding proteinAlternative name(s): Protein interacting with C kinase 1 Protein kinase C-alpha-binding protein |
Gene namesi | Name:Pick1 Synonyms:Prkcabp |
Organismi | Rattus norvegicus (Rat) |
Taxonomic identifieri | 10116 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Rattus |
Proteomesi |
|
Organism-specific databases
RGDi | 69437, Pick1 |
Subcellular locationi
Cytoskeleton
- cytoskeleton 1 Publication
Other locations
- perinuclear region 1 Publication
- Membrane 1 Publication; Peripheral membrane protein 1 Publication
- Membrane By similarity; Lipid-anchor By similarity
- postsynaptic density 1 Publication
- synaptosome 1 Publication
Note: Also membrane-associated, present at excitatory synapses.
Cytoskeleton
- cytoskeleton Source: UniProtKB-SubCell
Endosome
- postsynaptic early endosome Source: SynGO
Golgi apparatus
- Golgi apparatus Source: UniProtKB
- trans-Golgi network membrane Source: GO_Central
Mitochondrion
- mitochondrion Source: RGD
Plasma Membrane
- plasma membrane Source: UniProtKB
- presynaptic membrane Source: UniProtKB
Other locations
- cytoplasm Source: UniProtKB
- dendrite Source: RGD
- glutamatergic synapse Source: SynGO
- neuron projection Source: UniProtKB
- perinuclear region of cytoplasm Source: RGD
- postsynaptic density Source: UniProtKB
- postsynaptic endocytic zone Source: SynGO
- protein-containing complex Source: UniProtKB
- synapse Source: UniProtKB
- synaptic vesicle Source: SynGO
Keywords - Cellular componenti
Cell junction, Cytoplasm, Cytoskeleton, Membrane, Synapse, SynaptosomePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 – 28 | KD → AA: Abolishes interaction with other proteins, but not with itself. 4 Publications | 2 | |
Mutagenesisi | 44 | C → G: Decreased lipid membrane binding, but no effect on peptide ligand recognition. 1 Publication | 1 | |
Mutagenesisi | 46 | C → G: Decreased lipid membrane binding, but no effect on peptide ligand recognition. 1 Publication | 1 | |
Mutagenesisi | 79 | K → E: No effect on lipid membrane binding. 1 Publication | 1 | |
Mutagenesisi | 81 | K → E: No effect on lipid membrane binding. 1 Publication | 1 | |
Mutagenesisi | 251 – 252 | KK → EE: Abolishes F-actin binding and the inhibitory function on Arp2/3 complex-mediated actin nucleation, impairs lipid vesicle interaction, no effect on Arp2/3 complex association. 1 Publication | 2 | |
Mutagenesisi | 413 | W → A: Abolishes association with Arp2/3 complex and the inhibitory function on Arp2/3 complex-mediated actin nucleation. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000058429 | 1 – 416 | PRKCA-binding proteinAdd BLAST | 416 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 82 | PhosphothreonineBy similarity | 1 | |
Lipidationi | 414 | S-palmitoyl cysteine; by DHHC8By similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Lipoprotein, Palmitate, PhosphoproteinProteomic databases
jPOSTi | Q9EP80 |
PaxDbi | Q9EP80 |
PTM databases
iPTMneti | Q9EP80 |
PhosphoSitePlusi | Q9EP80 |
SwissPalmi | Q9EP80 |
Expressioni
Tissue specificityi
Developmental stagei
Interactioni
Subunit structurei
Monomer and homodimer.
Interacts with CXADR.
Interacts presynaptically with the glutamate receptors GRIA2, GRIA3, GRIK3, isoform 3 of GRIA4, isoform A of GRM4, GRM7 and GRM8; with NAPA and NAPB; and with BTG2. The interaction with NAPA and NAPB disrupts the interaction with GRIA2, conducting to the internalization of GRIA2.
Interacts with PRKCA; with the amine transporters SLC6A2 and SLC6A3; with the channels ASIC1 and ASIC2; with the GTP-binding proteins ARF1 and ARF3; with the ephrin receptor tyrosine kinases EPHA7, EPHB1 and EPHB2; with ERBB2 and through its PDZ domain with the C-terminal tail of PRLHR (By similarity).
Interacts with UNC5A.
Interacts (via AH domain) with NCS1/FREQ; in a calcium-dependent manner.
Interacts with F-actin and associates with the ARP2/3 complex.
Interacts (via PDZ domain) with ARF1 (activated); the interaction blocks Arp2/3 complex inhibition.
By similarity9 PublicationsBinary interactionsi
Hide detailsQ9EP80
GO - Molecular functioni
- actin filament binding Source: UniProtKB
- cytoskeletal protein binding Source: RGD
- enzyme binding Source: RGD
- ephrin receptor binding Source: UniProtKB
- G protein-coupled glutamate receptor binding Source: UniProtKB
- G protein-coupled receptor binding Source: ParkinsonsUK-UCL
- GTPase binding Source: UniProtKB
- identical protein binding Source: UniProtKB
- ionotropic glutamate receptor binding Source: UniProtKB
- protein C-terminus binding Source: RGD
- protein domain specific binding Source: RGD
- protein kinase C binding Source: UniProtKB
- receptor tyrosine kinase binding Source: UniProtKB
- signaling receptor binding Source: UniProtKB
Protein-protein interaction databases
BioGRIDi | 250021, 11 interactors |
CORUMi | Q9EP80 |
DIPi | DIP-30941N |
ELMi | Q9EP80 |
IntActi | Q9EP80, 14 interactors |
MINTi | Q9EP80 |
STRINGi | 10116.ENSRNOP00000016077 |
Structurei
Secondary structure
3D structure databases
SASBDBi | Q9EP80 |
SMRi | Q9EP80 |
ModBasei | Search... |
PDBe-KBi | Search... |
Miscellaneous databases
EvolutionaryTracei | Q9EP80 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 22 – 105 | PDZPROSITE-ProRule annotationAdd BLAST | 84 | |
Domaini | 144 – 357 | AHPROSITE-ProRule annotationAdd BLAST | 214 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 382 – 389 | Poly-Glu | 8 |
Domaini
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
InParanoidi | Q9EP80 |
OrthoDBi | 545640at2759 |
PhylomeDBi | Q9EP80 |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
10 20 30 40 50
MFADLDYDIE EDKLGIPTVP GKVTLQKDAQ NLIGISIGGG AQYCPCLYIV
60 70 80 90 100
QVFDNTPAAL DGTVAAGDEI TGVNGRSIKG KTKVEVAKMI QEVKGEVTIH
110 120 130 140 150
YNKLQADPKQ GMSLDIVLKK VKHRLVENMS SGTADALGLS RAILCNDGLV
160 170 180 190 200
KRLEELERTA ELYKGMTEHT KNLLRAFYEL SQTHRAFGDV FSVIGVREPQ
210 220 230 240 250
PAASEAFVKF ADAHRSIEKF GIRLLKTIKP MLTDLNTYLN KAIPDTRLTI
260 270 280 290 300
KKYLDVKFEY LSYCLKVKEM DDEEYSCIAL GEPLYRVSTG NYEYRLILRC
310 320 330 340 350
RQEARARFSQ MRKDVLEKME LLDQKHVQDI VFQLQRFVST MSKYYNDCYA
360 370 380 390 400
VLRDADVFPI EVDLAHTTLA YGPNQGGFTD GEDEEEEEED GAAREVSKDA
410
RGATGPTDKG GSWCDS
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ6GQQ2 | Q6GQQ2_RAT | PRKCA-binding protein | Pick1 rCG_60080 | 416 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 401 – 402 | RG → TW in AAK54603 (PubMed:11237868).Curated | 2 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327562 mRNA Translation: AAG48152.1 AJ240083 mRNA Translation: CAC17808.2 AF373289 mRNA Translation: AAK54603.1 AF542094 mRNA Translation: AAO49507.1 |
RefSeqi | NP_445912.1, NM_053460.1 |
Genome annotation databases
GeneIDi | 84591 |
KEGGi | rno:84591 |
UCSCi | RGD:69437, rat |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF327562 mRNA Translation: AAG48152.1 AJ240083 mRNA Translation: CAC17808.2 AF373289 mRNA Translation: AAK54603.1 AF542094 mRNA Translation: AAO49507.1 |
RefSeqi | NP_445912.1, NM_053460.1 |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
2LUI | NMR | - | A | 19-110 | [»] | |
2PKU | NMR | - | A | 18-104 | [»] | |
3HPK | X-ray | 2.20 | A/B | 1-110 | [»] | |
3HPM | X-ray | 2.80 | A/B | 19-110 | [»] | |
SASBDBi | Q9EP80 | |||||
SMRi | Q9EP80 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 250021, 11 interactors |
CORUMi | Q9EP80 |
DIPi | DIP-30941N |
ELMi | Q9EP80 |
IntActi | Q9EP80, 14 interactors |
MINTi | Q9EP80 |
STRINGi | 10116.ENSRNOP00000016077 |
PTM databases
iPTMneti | Q9EP80 |
PhosphoSitePlusi | Q9EP80 |
SwissPalmi | Q9EP80 |
Proteomic databases
jPOSTi | Q9EP80 |
PaxDbi | Q9EP80 |
Protocols and materials databases
ABCDi | Q9EP80, 1 sequenced antibody |
Genome annotation databases
GeneIDi | 84591 |
KEGGi | rno:84591 |
UCSCi | RGD:69437, rat |
Organism-specific databases
CTDi | 9463 |
RGDi | 69437, Pick1 |
Phylogenomic databases
eggNOGi | KOG3651, Eukaryota |
InParanoidi | Q9EP80 |
OrthoDBi | 545640at2759 |
PhylomeDBi | Q9EP80 |
Enzyme and pathway databases
Reactomei | R-RNO-416993, Trafficking of GluR2-containing AMPA receptors |
Miscellaneous databases
EvolutionaryTracei | Q9EP80 |
PROi | PR:Q9EP80 |
Family and domain databases
CDDi | cd07659, BAR_PICK1, 1 hit |
Gene3Di | 1.20.1270.60, 1 hit 2.30.42.10, 1 hit |
InterProi | View protein in InterPro IPR027267, AH/BAR_dom_sf IPR010504, AH_dom IPR030798, Arfaptin_fam IPR001478, PDZ IPR036034, PDZ_sf IPR037959, PICK1_BAR |
PANTHERi | PTHR12141, PTHR12141, 1 hit |
Pfami | View protein in Pfam PF06456, Arfaptin, 1 hit PF00595, PDZ, 1 hit |
SMARTi | View protein in SMART SM01015, Arfaptin, 1 hit SM00228, PDZ, 1 hit |
SUPFAMi | SSF103657, SSF103657, 1 hit SSF50156, SSF50156, 1 hit |
PROSITEi | View protein in PROSITE PS50870, AH, 1 hit PS50106, PDZ, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PICK1_RAT | |
Accessioni | Q9EP80Primary (citable) accession number: Q9EP80 Secondary accession number(s): Q546X4, Q925D1 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 2, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 161 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- PDB cross-references
Index of Protein Data Bank (PDB) cross-references