Cd274

Functionⁱ

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity). May costimulate helper T-cell subsets through a yet unknown activating receptor (PubMed:11015443, PubMed:12719480).By similarity3 Publications

GO - Biological processⁱ

cell surface receptor signaling pathway Source: MGI
immune response Source: MGI
negative regulation of activated T cell proliferation Source: MGI
negative regulation of CD4-positive, alpha-beta T cell proliferation Source: MGI
negative regulation of CD8-positive, alpha-beta T cell activation Source: UniProtKB
negative regulation of interferon-gamma production Source: MGI
negative regulation of interleukin-10 production Source: MGI
negative regulation of T cell proliferation
Source: MGI
Inferred from direct assayⁱ
- 17016562
negative regulation of tumor necrosis factor superfamily cytokine production Source: MGI
positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: MGI
positive regulation of cell migration Source: MGI
positive regulation of interleukin-10 secretion Source: MGI
positive regulation of T cell proliferation Source: MGI
positive regulation of tolerance induction to tumor cell Source: UniProtKB
response to cytokine Source: MGI
signal transduction Source: MGI
T cell costimulation Source: MGI
toxin transport
Source: MGI
Inferred from mutant phenotypeⁱ
- 23716698

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Receptor
Biological process	Immunity

Enzyme and pathway databases

Reactomeⁱ	R-MMU-389948 PD-1 signaling

Reactomeⁱ

R-MMU-389948 PD-1 signaling

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Programmed cell death 1 ligand 1 Short name: PD-L1 Short name: PDCD1 ligand 1 Short name: Programmed death ligand 1 Alternative name(s): B7 homolog 1 Short name: B7-H1 CD_antigen: CD274
Gene namesⁱ	Name:Cd274 Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
Organismⁱ	Mus musculus (Mouse)
Taxonomic identifierⁱ	10090 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Boreoeutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus
Proteomesⁱ	UP000000589 Componentⁱ: Chromosome 19

Organism-specific databases

MGIⁱ	MGI:1926446 Cd274

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	19 – 239	ExtracellularSequence analysisAdd BLAST	221
Transmembraneⁱ	240 – 260	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	261 – 290	CytoplasmicSequence analysisAdd BLAST	30

Keywords - Cellular componentⁱ

Cell membrane, Endosome, Membrane

Pathology & Biotechⁱ

Mutagenesis

Feature key	Position(s)	DescriptionActions	Length
Mutagenesisⁱ	27	L → A: PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	31	E → S: Significantly reduces the binding to PDCD1. 1 Publication	1
Mutagenesisⁱ	34	S → Y: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	37	T → Y: Significantly reduces the binding to PDCD1. 1 Publication	1
Mutagenesisⁱ	49	D → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	56	Y → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	58	E → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	62	E → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	67	F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication	1
Mutagenesisⁱ	69	A → F: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	72	E → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	75	K → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	89	K → S: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	98	A → F: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	100	Q → S: No effect on PDCD1 binding.	1
Mutagenesisⁱ	113	C → Y: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	115	I → A: Abolishes the binding to PDCD1. 1 Publication	1
Mutagenesisⁱ	117	S → Y: No effect on PDCD1 binding. 1 Publication	1
Mutagenesisⁱ	124	K → A: Abolishes the binding to PDCD1. 1 Publication	1
Mutagenesisⁱ	126	I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication	1
Mutagenesisⁱ	129	K → S: Abolishes the binding to PDCD1. 1 Publication	1

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Signal peptideⁱ	1 – 18	Sequence analysisAdd BLAST		18
ChainⁱPRO_0000014554	19 – 290	Programmed cell death 1 ligand 1Add BLAST		272

Amino acid modifications

Feature key	Position(s)	DescriptionActions	Length
Glycosylationⁱ	35	N-linked (GlcNAc...) asparagineSequence analysis	1
Disulfide bondⁱ	40 ↔ 114	PROSITE-ProRule annotation
Disulfide bondⁱ	154 ↔ 208	PROSITE-ProRule annotation
Glycosylationⁱ	191	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	199	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	218	N-linked (GlcNAc...) asparagineSequence analysis	1
Glycosylationⁱ	236	N-linked (GlcNAc...) asparagineSequence analysis	1

Post-translational modificationⁱ

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.By similarity

Keywords - PTMⁱ

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

MaxQB - The MaxQuant DataBase More...MaxQBⁱ	Q9EP73
PaxDbⁱ	Q9EP73
PRIDEⁱ	Q9EP73

PTM databases

PhosphoSitePlusⁱ	Q9EP73

PhosphoSitePlusⁱ

Q9EP73

Expressionⁱ

Tissue specificityⁱ

Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications

Inductionⁱ

Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and IFNG.2 Publications

Gene expression databases

Bgeeⁱ	ENSMUSG00000016496 Expressed in 101 organ(s), highest expression level in blood
CleanExⁱ	MM_CD274
ExpressionAtlasⁱ	Q9EP73 baseline and differential
Genevisibleⁱ	Q9EP73 MM

Interactionⁱ

Subunit structureⁱ

Binary interactionsⁱ

With	Entry	#Exp.	IntAct	Notes
Cd80	Q00609	5	EBI-5258879,EBI-5258929
Pdcd1	Q02242	3	EBI-5258879,EBI-5258903

Protein-protein interaction databases

Database of interacting proteins More...DIPⁱ	DIP-46167N
IntActⁱ	Q9EP73, 2 interactors
STRINGⁱ	10090.ENSMUSP00000016640

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9EP73
SMRⁱ	Q9EP73
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	19 – 127	Ig-like V-typeAdd BLAST		109
Domainⁱ	133 – 224	Ig-like C2-typeAdd BLAST		92

Sequence similaritiesⁱ

Belongs to the immunoglobulin superfamily. BTN/MOG family.Curated

Keywords - Domainⁱ

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	ENOG410IIBU Eukaryota ENOG4111V14 LUCA
Ensembl GeneTree More...GeneTreeⁱ	ENSGT00650000093373
HOGENOMⁱ	HOG000059625
HOVERGENⁱ	HBG082112
InParanoidⁱ	Q9EP73
KEGG Orthology (KO) More...KOⁱ	K06745
OMAⁱ	IWTSSDH
Database of Orthologous Groups More...OrthoDBⁱ	EOG091G0F5W
PhylomeDBⁱ	Q9EP73
TreeFamⁱ	TF331083

Family and domain databases

Gene3Dⁱ	2.60.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR013162 CD80_C2-set IPR007110 Ig-like_dom IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR003599 Ig_sub IPR013106 Ig_V-set
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF08205 C2-set_2, 1 hit PF07686 V-set, 1 hit
SMARTⁱ	View protein in SMART SM00409 IG, 2 hits
SUPFAMⁱ	SSF48726 SSF48726, 2 hits
PROSITEⁱ	View protein in PROSITE PS50835 IG_LIKE, 2 hits

Sequenceⁱ

Sequence statusⁱ: Complete.

Sequence processingⁱ: The displayed sequence is further processed into a mature form.

Q9EP73-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL 
        60         70         80         90        100
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ 
       110        120        130        140        150
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH 
       160        170        180        190        200
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA 
       210        220        230        240        250
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL 
       260        270        280        290
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET

Length:290

Mass (Da):32,780

Last modified:March 1, 2001 - v1

Checksum:ⁱAB7C46CF853EBB02

GO

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF233517 mRNA Translation: AAG18509.1 AF317088 mRNA Translation: AAG31810.1 BC066841 mRNA Translation: AAH66841.1
The Consensus CDS (CCDS) project More...CCDSⁱ	CCDS29735.1
NCBI Reference Sequences More...RefSeqⁱ	NP_068693.1, NM_021893.3
UniGeneⁱ	Mm.245363

Genome annotation databases

Ensemblⁱ	ENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496
GeneIDⁱ	60533
KEGGⁱ	mmu:60533
UCSC genome browser More...UCSCⁱ	uc008hdi.2 mouse

Similar proteinsⁱ

100% Identity
90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9EP73	CD274 antigen		MOUSE		290	UniRef100_Q9EP73

Protein	Similar proteins		Species	Score	Length	Source
Q9EP73	CD274 antigen		MOUSE		290	UniRef90_Q9EP73
PD-L1		MUSSP		290
UPI000A3114BB		MUSPA		290
UPI000A31646B		MUSCR		290

Protein	Similar proteins		Species	Score	Length	Source
Q9EP73	Programmed cell death 1 ligand 1	)	HUMAN		290	UniRef50_Q9NZQ7
CD274 isoform 2		PANTR		290
Uncharacterized protein		PANPA		290
CD274 isoform 2		PONAB		290
CD274 molecule		GORGO		290
+61

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF233517 mRNA Translation: AAG18509.1 AF317088 mRNA Translation: AAG31810.1 BC066841 mRNA Translation: AAH66841.1
CCDSⁱ	CCDS29735.1
RefSeqⁱ	NP_068693.1, NM_021893.3
UniGeneⁱ	Mm.245363

3D structure databases

ProteinModelPortalⁱ	Q9EP73
SMRⁱ	Q9EP73
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

DIPⁱ	DIP-46167N
IntActⁱ	Q9EP73, 2 interactors
STRINGⁱ	10090.ENSMUSP00000016640

PTM databases

PhosphoSitePlusⁱ	Q9EP73

PhosphoSitePlusⁱ

Q9EP73

Proteomic databases

MaxQBⁱ	Q9EP73
PaxDbⁱ	Q9EP73
PRIDEⁱ	Q9EP73

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

Ensemblⁱ	ENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496
GeneIDⁱ	60533
KEGGⁱ	mmu:60533
UCSCⁱ	uc008hdi.2 mouse

Organism-specific databases

CTDⁱ	29126
MGIⁱ	MGI:1926446 Cd274

Phylogenomic databases

eggNOGⁱ	ENOG410IIBU Eukaryota ENOG4111V14 LUCA
GeneTreeⁱ	ENSGT00650000093373
HOGENOMⁱ	HOG000059625
HOVERGENⁱ	HBG082112
InParanoidⁱ	Q9EP73
KOⁱ	K06745
OMAⁱ	IWTSSDH
OrthoDBⁱ	EOG091G0F5W
PhylomeDBⁱ	Q9EP73
TreeFamⁱ	TF331083

Enzyme and pathway databases

Reactomeⁱ	R-MMU-389948 PD-1 signaling

Reactomeⁱ

R-MMU-389948 PD-1 signaling

Miscellaneous databases

ChiTaRSⁱ	Cd274 mouse
Protein Ontology More...PROⁱ	PR:Q9EP73
SOURCEⁱ	Search...

Gene expression databases

Bgeeⁱ	ENSMUSG00000016496 Expressed in 101 organ(s), highest expression level in blood
CleanExⁱ	MM_CD274
ExpressionAtlasⁱ	Q9EP73 baseline and differential
Genevisibleⁱ	Q9EP73 MM

Family and domain databases

Gene3Dⁱ	2.60.40.10, 2 hits
InterProⁱ	View protein in InterPro IPR013162 CD80_C2-set IPR007110 Ig-like_dom IPR036179 Ig-like_dom_sf IPR013783 Ig-like_fold IPR003599 Ig_sub IPR013106 Ig_V-set
Pfamⁱ	View protein in Pfam PF08205 C2-set_2, 1 hit PF07686 V-set, 1 hit
SMARTⁱ	View protein in SMART SM00409 IG, 2 hits
SUPFAMⁱ	SSF48726 SSF48726, 2 hits
PROSITEⁱ	View protein in PROSITE PS50835 IG_LIKE, 2 hits
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	PD1L1_MOUSE
Accessionⁱ	Q9EP73Primary (citable) accession number: Q9EP73
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	May 10, 2005
	Last sequence update:	March 1, 2001
	Last modified:	October 10, 2018
	This is version 129 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

Documents

SIMILARITY comments
Index of protein domains and families
MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9EP73 (PD1L1_MOUSE)

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Programmed cell death 1 ligand 1

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Biological processi

Enzyme and pathway databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Plasma membrane

Endosome

Endosome

Extracellular region or secreted

Plasma Membrane

Other locations

Topology

Keywords - Cellular componenti

<p>This section provides information on the disease(s) and phenotype(s) associated with a protein.<p><a href='/help/pathology_and_biotech_section' target='_top'>More...</a></p>Pathology & Biotechi

Mutagenesis

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Amino acid modifications

Keywords - PTMi

Proteomic databases

PTM databases

<p>This section provides information on the expression of a gene at the mRNA or protein level in cells or in tissues of multicellular organisms.<p><a href='/help/expression_section' target='_top'>More...</a></p>Expressioni

Gene expression databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Gene expression databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

Documents

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Functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

Pathology & Biotechⁱ

PTM / Processingⁱ

Keywords - PTMⁱ

Expressionⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ