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Protein

Programmed cell death 1 ligand 1

Gene

Cd274

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a critical role in induction and maintenance of immune tolerance to self. As a ligand for the inhibitory receptor PDCD1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124). The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and facilitate tumor survival (By similarity). May costimulate helper T-cell subsets through a yet unknown activating receptor (PubMed:11015443, PubMed:12719480).By similarity3 Publications

GO - Biological processi

Keywordsi

Molecular functionReceptor
Biological processImmunity

Enzyme and pathway databases

ReactomeiR-MMU-389948 PD-1 signaling

Names & Taxonomyi

Protein namesi
Recommended name:
Programmed cell death 1 ligand 1
Short name:
PD-L1
Short name:
PDCD1 ligand 1
Short name:
Programmed death ligand 1
Alternative name(s):
B7 homolog 1
Short name:
B7-H1
CD_antigen: CD274
Gene namesi
Name:Cd274
Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 19

Organism-specific databases

MGIiMGI:1926446 Cd274

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini19 – 239ExtracellularSequence analysisAdd BLAST221
Transmembranei240 – 260HelicalSequence analysisAdd BLAST21
Topological domaini261 – 290CytoplasmicSequence analysisAdd BLAST30

Keywords - Cellular componenti

Cell membrane, Endosome, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi27L → A: PDCD1 binding. 1 Publication1
Mutagenesisi31E → S: Significantly reduces the binding to PDCD1. 1 Publication1
Mutagenesisi34S → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi37T → Y: Significantly reduces the binding to PDCD1. 1 Publication1
Mutagenesisi49D → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi56Y → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi58E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi62E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi67F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication1
Mutagenesisi69A → F: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi72E → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi75K → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi89K → S: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi98A → F: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi100Q → S: No effect on PDCD1 binding. 1
Mutagenesisi113C → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi115I → A: Abolishes the binding to PDCD1. 1 Publication1
Mutagenesisi117S → Y: No effect on PDCD1 binding. 1 Publication1
Mutagenesisi124K → A: Abolishes the binding to PDCD1. 1 Publication1
Mutagenesisi126I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication1
Mutagenesisi129K → S: Abolishes the binding to PDCD1. 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 18Sequence analysisAdd BLAST18
ChainiPRO_000001455419 – 290Programmed cell death 1 ligand 1Add BLAST272

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi35N-linked (GlcNAc...) asparagineSequence analysis1
Disulfide bondi40 ↔ 114PROSITE-ProRule annotation
Disulfide bondi154 ↔ 208PROSITE-ProRule annotation
Glycosylationi191N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi199N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi218N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi236N-linked (GlcNAc...) asparagineSequence analysis1

Post-translational modificationi

Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein, Ubl conjugation

Proteomic databases

MaxQBiQ9EP73
PaxDbiQ9EP73
PRIDEiQ9EP73

PTM databases

PhosphoSitePlusiQ9EP73

Expressioni

Tissue specificityi

Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications

Inductioni

Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and IFNG.2 Publications

Gene expression databases

BgeeiENSMUSG00000016496
CleanExiMM_CD274
ExpressionAtlasiQ9EP73 baseline and differential
GenevisibleiQ9EP73 MM

Interactioni

Subunit structurei

Interacts with PDCD1 (PubMed:11015443). Interacts with CMTM4 and CMTM6 (By similarity).By similarity1 Publication

Binary interactionsi

Show more details

Protein-protein interaction databases

DIPiDIP-46167N
IntActiQ9EP73, 2 interactors
STRINGi10090.ENSMUSP00000016640

Structurei

3D structure databases

ProteinModelPortaliQ9EP73
SMRiQ9EP73
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini19 – 127Ig-like V-typeAdd BLAST109
Domaini133 – 224Ig-like C2-typeAdd BLAST92

Sequence similaritiesi

Keywords - Domaini

Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG410IIBU Eukaryota
ENOG4111V14 LUCA
GeneTreeiENSGT00650000093373
HOGENOMiHOG000059625
HOVERGENiHBG082112
InParanoidiQ9EP73
KOiK06745
OMAiIWTSSDH
OrthoDBiEOG091G0F5W
PhylomeDBiQ9EP73
TreeFamiTF331083

Family and domain databases

Gene3Di2.60.40.10, 2 hits
InterProiView protein in InterPro
IPR013162 CD80_C2-set
IPR007110 Ig-like_dom
IPR036179 Ig-like_dom_sf
IPR013783 Ig-like_fold
IPR003599 Ig_sub
IPR013106 Ig_V-set
PfamiView protein in Pfam
PF08205 C2-set_2, 1 hit
PF07686 V-set, 1 hit
SMARTiView protein in SMART
SM00409 IG, 2 hits
SUPFAMiSSF48726 SSF48726, 2 hits
PROSITEiView protein in PROSITE
PS50835 IG_LIKE, 2 hits

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EP73-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL
60 70 80 90 100
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ
110 120 130 140 150
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH
160 170 180 190 200
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA
210 220 230 240 250
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL
260 270 280 290
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET
Length:290
Mass (Da):32,780
Last modified:March 1, 2001 - v1
Checksum:iAB7C46CF853EBB02
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF233517 mRNA Translation: AAG18509.1
AF317088 mRNA Translation: AAG31810.1
BC066841 mRNA Translation: AAH66841.1
CCDSiCCDS29735.1
RefSeqiNP_068693.1, NM_021893.3
UniGeneiMm.245363

Genome annotation databases

EnsembliENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496
GeneIDi60533
KEGGimmu:60533
UCSCiuc008hdi.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPD1L1_MOUSE
AccessioniPrimary (citable) accession number: Q9EP73
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2005
Last sequence update: March 1, 2001
Last modified: June 20, 2018
This is version 126 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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