UniProtKB - Q9EP73 (PD1L1_MOUSE)
Protein
Programmed cell death 1 ligand 1
Gene
Cd274
Organism
Mus musculus (Mouse)
Status
Functioni
Plays a critical role in induction and maintenance of immune tolerance to self (PubMed:11238124). As a ligand for the inhibitory receptor PDCD1/PD-1, modulates the activation threshold of T-cells and limits T-cell effector response (PubMed:11238124). Through a yet unknown activating receptor, may costimulate T-cell subsets that predominantly produce interleukin-10 (IL10) (PubMed:11015443, PubMed:12719480).3 Publications
The PDCD1-mediated inhibitory pathway is exploited by tumors to attenuate anti-tumor immunity and escape destruction by the immune system, thereby facilitating tumor survival (PubMed:12218188, PubMed:27281199). The interaction with PDCD1/PD-1 inhibits cytotoxic T lymphocytes (CTLs) effector function (PubMed:12218188). The blockage of the PDCD1-mediated pathway results in the reversal of the exhausted T-cell phenotype and the normalization of the anti-tumor response, providing a rationale for cancer immunotherapy (PubMed:12218188).2 Publications
GO - Biological processi
- adaptive immune response Source: UniProtKB-KW
- cell surface receptor signaling pathway Source: MGI
- cellular response to lipopolysaccharide Source: GO_Central
- immune response Source: MGI
- negative regulation of activated T cell proliferation Source: MGI
- negative regulation of CD4-positive, alpha-beta T cell proliferation Source: MGI
- negative regulation of CD8-positive, alpha-beta T cell activation Source: UniProtKB
- negative regulation of interferon-gamma production Source: MGI
- negative regulation of interleukin-10 production Source: MGI
- negative regulation of T cell proliferation Source: MGI
- negative regulation of tumor necrosis factor superfamily cytokine production Source: MGI
- positive regulation of activated CD8-positive, alpha-beta T cell apoptotic process Source: MGI
- positive regulation of cell migration Source: MGI
- positive regulation of interleukin-10 production Source: MGI
- positive regulation of T cell proliferation Source: MGI
- positive regulation of tolerance induction to tumor cell Source: UniProtKB
- response to cytokine Source: MGI
- signal transduction Source: MGI
- T cell costimulation Source: MGI
- toxin transport Source: MGI
Keywordsi
Molecular function | Receptor |
Biological process | Adaptive immunity, Immunity |
Enzyme and pathway databases
Reactomei | R-MMU-389948, PD-1 signaling |
Names & Taxonomyi
Protein namesi | Recommended name: Programmed cell death 1 ligand 1Short name: PD-L1 Short name: PDCD1 ligand 1 Short name: Programmed death ligand 1 Alternative name(s): B7 homolog 1 Short name: B7-H1 CD_antigen: CD274 |
Gene namesi | Name:Cd274 Synonyms:B7h1, Pdcd1l1, Pdcd1lg1, Pdl1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1926446, Cd274 |
Subcellular locationi
Endosome
- Early endosome membrane By similarity; Single-pass type I membrane protein Sequence analysis
- Recycling endosome membrane By similarity; Single-pass type I membrane protein Sequence analysis
Plasma membrane
- Cell membrane By similarity; Single-pass type I membrane protein Sequence analysis
Endosome
- early endosome membrane Source: UniProtKB
- recycling endosome membrane Source: UniProtKB
Extracellular region or secreted
- extracellular exosome Source: MGI
Plasma Membrane
- external side of plasma membrane Source: MGI
- plasma membrane Source: MGI
Other locations
- cell surface Source: MGI
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 19 – 239 | ExtracellularSequence analysisAdd BLAST | 221 | |
Transmembranei | 240 – 260 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 261 – 290 | CytoplasmicSequence analysisAdd BLAST | 30 |
Keywords - Cellular componenti
Cell membrane, Endosome, MembranePathology & Biotechi
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 27 | L → A: PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 31 | E → S: Significantly reduces the binding to PDCD1. 1 Publication | 1 | |
Mutagenesisi | 34 | S → Y: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 37 | T → Y: Significantly reduces the binding to PDCD1. 1 Publication | 1 | |
Mutagenesisi | 49 | D → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 56 | Y → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 58 | E → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 62 | E → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 67 | F → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication | 1 | |
Mutagenesisi | 69 | A → F: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 72 | E → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 75 | K → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 89 | K → S: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 98 | A → F: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 100 | Q → S: No effect on PDCD1 binding. | 1 | |
Mutagenesisi | 113 | C → Y: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 115 | I → A: Abolishes the binding to PDCD1. 1 Publication | 1 | |
Mutagenesisi | 117 | S → Y: No effect on PDCD1 binding. 1 Publication | 1 | |
Mutagenesisi | 124 | K → A: Abolishes the binding to PDCD1. 1 Publication | 1 | |
Mutagenesisi | 126 | I → A: Abolishes the binding to PDCD1. Costimulates proliferation and IFNG production of T-cells. 1 Publication | 1 | |
Mutagenesisi | 129 | K → S: Abolishes the binding to PDCD1. 1 Publication | 1 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 18 | Sequence analysisAdd BLAST | 18 | |
ChainiPRO_0000014554 | 19 – 290 | Programmed cell death 1 ligand 1Add BLAST | 272 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Glycosylationi | 35 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 40 ↔ 114 | PROSITE-ProRule annotation | ||
Disulfide bondi | 154 ↔ 208 | PROSITE-ProRule annotation | ||
Glycosylationi | 191 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 199 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 218 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 236 | N-linked (GlcNAc...) asparagineSequence analysis | 1 |
Post-translational modificationi
Ubiquitinated; STUB1 likely mediates polyubiquitination of PD-L1/CD274 triggering its degradation.By similarity
Keywords - PTMi
Disulfide bond, Glycoprotein, Ubl conjugationProteomic databases
MaxQBi | Q9EP73 |
PaxDbi | Q9EP73 |
PRIDEi | Q9EP73 |
PTM databases
GlyGeni | Q9EP73, 5 sites |
iPTMneti | Q9EP73 |
PhosphoSitePlusi | Q9EP73 |
SwissPalmi | Q9EP73 |
Expressioni
Tissue specificityi
Highly expressed in the heart, thymus, skeletal muscle, and lung. Weakly expressed in the kidney, spleen, thyroid, and liver. Expressed on activated dendritic cells, B-cells and macrophages. Expressed in numerous tumor cells lines of lymphoid origin.3 Publications
Inductioni
Up-regulated by IFNG treatment in monocytes. Up-regulated on dendritic cells, B-cells and macrophages after activation by LPS and IFNG.2 Publications
Gene expression databases
Bgeei | ENSMUSG00000016496, Expressed in blood and 126 other tissues |
ExpressionAtlasi | Q9EP73, baseline and differential |
Genevisiblei | Q9EP73, MM |
Interactioni
Subunit structurei
Interacts with PDCD1 (PubMed:11015443).
Interacts with CMTM4 and CMTM6 (By similarity).
By similarity1 PublicationBinary interactionsi
Hide detailsProtein-protein interaction databases
DIPi | DIP-46167N |
IntActi | Q9EP73, 2 interactors |
STRINGi | 10090.ENSMUSP00000016640 |
Miscellaneous databases
RNActi | Q9EP73, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 19 – 127 | Ig-like V-typeAdd BLAST | 109 | |
Domaini | 133 – 224 | Ig-like C2-typeAdd BLAST | 92 |
Sequence similaritiesi
Keywords - Domaini
Immunoglobulin domain, Repeat, Signal, Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | ENOG502S1Y9, Eukaryota |
GeneTreei | ENSGT00940000161430 |
HOGENOMi | CLU_013137_8_3_1 |
InParanoidi | Q9EP73 |
OMAi | CTFRRLG |
OrthoDBi | 892262at2759 |
PhylomeDBi | Q9EP73 |
TreeFami | TF331083 |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9EP73-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MRIFAGIIFT ACCHLLRAFT ITAPKDLYVV EYGSNVTMEC RFPVERELDL
60 70 80 90 100
LALVVYWEKE DEQVIQFVAG EEDLKPQHSN FRGRASLPKD QLLKGNAALQ
110 120 130 140 150
ITDVKLQDAG VYCCIISYGG ADYKRITLKV NAPYRKINQR ISVDPATSEH
160 170 180 190 200
ELICQAEGYP EAEVIWTNSD HQPVSGKRSV TTSRTEGMLL NVTSSLRVNA
210 220 230 240 250
TANDVFYCTF WRSQPGQNHT AELIIPELPA THPPQNRTHW VLLGSILLFL
260 270 280 290
IVVSTVLLFL RKQVRMLDVE KCGVEDTSSK NRNDTQFEET
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233517 mRNA Translation: AAG18509.1 AF317088 mRNA Translation: AAG31810.1 BC066841 mRNA Translation: AAH66841.1 |
CCDSi | CCDS29735.1 |
RefSeqi | NP_068693.1, NM_021893.3 |
Genome annotation databases
Ensembli | ENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496 |
GeneIDi | 60533 |
KEGGi | mmu:60533 |
UCSCi | uc008hdi.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233517 mRNA Translation: AAG18509.1 AF317088 mRNA Translation: AAG31810.1 BC066841 mRNA Translation: AAH66841.1 |
CCDSi | CCDS29735.1 |
RefSeqi | NP_068693.1, NM_021893.3 |
3D structure databases
SMRi | Q9EP73 |
ModBasei | Search... |
Protein-protein interaction databases
DIPi | DIP-46167N |
IntActi | Q9EP73, 2 interactors |
STRINGi | 10090.ENSMUSP00000016640 |
PTM databases
GlyGeni | Q9EP73, 5 sites |
iPTMneti | Q9EP73 |
PhosphoSitePlusi | Q9EP73 |
SwissPalmi | Q9EP73 |
Proteomic databases
MaxQBi | Q9EP73 |
PaxDbi | Q9EP73 |
PRIDEi | Q9EP73 |
Protocols and materials databases
ABCDi | Q9EP73, 66 sequenced antibodies |
Antibodypediai | 24139, 1764 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000016640; ENSMUSP00000016640; ENSMUSG00000016496 |
GeneIDi | 60533 |
KEGGi | mmu:60533 |
UCSCi | uc008hdi.2, mouse |
Organism-specific databases
CTDi | 29126 |
MGIi | MGI:1926446, Cd274 |
Phylogenomic databases
eggNOGi | ENOG502S1Y9, Eukaryota |
GeneTreei | ENSGT00940000161430 |
HOGENOMi | CLU_013137_8_3_1 |
InParanoidi | Q9EP73 |
OMAi | CTFRRLG |
OrthoDBi | 892262at2759 |
PhylomeDBi | Q9EP73 |
TreeFami | TF331083 |
Enzyme and pathway databases
Reactomei | R-MMU-389948, PD-1 signaling |
Miscellaneous databases
BioGRID-ORCSi | 60533, 2 hits in 21 CRISPR screens |
ChiTaRSi | Cd274, mouse |
PROi | PR:Q9EP73 |
RNActi | Q9EP73, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000016496, Expressed in blood and 126 other tissues |
ExpressionAtlasi | Q9EP73, baseline and differential |
Genevisiblei | Q9EP73, MM |
Family and domain databases
Gene3Di | 2.60.40.10, 2 hits |
InterProi | View protein in InterPro IPR013162, CD80_C2-set IPR007110, Ig-like_dom IPR036179, Ig-like_dom_sf IPR013783, Ig-like_fold IPR003599, Ig_sub IPR013106, Ig_V-set |
Pfami | View protein in Pfam PF08205, C2-set_2, 1 hit PF07686, V-set, 1 hit |
SMARTi | View protein in SMART SM00409, IG, 2 hits |
SUPFAMi | SSF48726, SSF48726, 2 hits |
PROSITEi | View protein in PROSITE PS50835, IG_LIKE, 2 hits |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PD1L1_MOUSE | |
Accessioni | Q9EP73Primary (citable) accession number: Q9EP73 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2005 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 145 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families