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Protein

Ankycorbin

Gene

Rai14

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Plays a role in actin regulation at the ectoplasmic specialization, a type of cell junction specific to testis. Important for establishment of sperm polarity and normal spermatid adhesion. May also promote integrity of Sertoli cell tight junctions at the blood-testis barrier.By similarity

GO - Biological processi

Keywordsi

Biological processDifferentiation, Spermatogenesis

Names & Taxonomyi

Protein namesi
Recommended name:
Ankycorbin
Alternative name(s):
Ankyrin repeat and coiled-coil structure-containing protein
Novel retinal pigment epithelial cell protein
Retinoic acid-induced protein 14
p125
Gene namesi
Name:Rai14
Synonyms:Kiaa1334, Norpeg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 15

Organism-specific databases

MGIiMGI:1922896 Rai14

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cell junction, Cytoplasm, Cytoskeleton, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002396311 – 979AnkycorbinAdd BLAST979

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei11PhosphoserineBy similarity1
Modified residuei249PhosphothreonineBy similarity1
Modified residuei281PhosphoserineBy similarity1
Modified residuei286PhosphoserineBy similarity1
Modified residuei293PhosphoserineBy similarity1
Modified residuei295PhosphothreonineBy similarity1
Modified residuei297PhosphothreonineBy similarity1
Modified residuei300PhosphoserineBy similarity1
Modified residuei304PhosphoserineCombined sources1
Modified residuei318PhosphoserineBy similarity1
Modified residuei327PhosphoserineBy similarity1
Modified residuei329PhosphoserineBy similarity1
Modified residuei340PhosphoserineBy similarity1
Modified residuei341PhosphoserineBy similarity1
Modified residuei358PhosphoserineBy similarity1
Modified residuei513PhosphoserineBy similarity1
Modified residuei516PhosphoserineBy similarity1
Modified residuei667PhosphoserineBy similarity1
Modified residuei694PhosphoserineCombined sources1
Modified residuei914PhosphoserineCombined sources1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9EP71
MaxQBiQ9EP71
PaxDbiQ9EP71
PeptideAtlasiQ9EP71
PRIDEiQ9EP71

PTM databases

iPTMnetiQ9EP71
PhosphoSitePlusiQ9EP71

Expressioni

Tissue specificityi

Highly expressed in testis, where it localizes to seminiferous tubules (at protein level) (PubMed:11042181, PubMed:16806700). Expressed in ganglion cell layer and in Muller cell fibers of the retina (at protein level) (PubMed:11042181, PubMed:16806700). In small intestine highly expressed at the apical and lateral borders of absorptive epithelia (at protein level) (PubMed:11168586). In liver highly expressed along the bile canaliculi (at protein level) (PubMed:11168586).3 Publications

Developmental stagei

At 9.5 days, expression is detected in branchial arch mesenchyme, forebrain, hindbrain, midbrain, and neural tube. At 12.5 days, is detected in the hindbrain, forebrain, lung, genital eminence, spinal ligaments around vertebrae and ribs, and around the cartilage of ribs and nasal sinuses. Also detected in frontonasal mass, mandibular arch, optic sulcus, spinal ganglia and hind limb bud. At 15.5 days, expression is detected in the ventricular layer of neurons subjacent to the neocortex, around the nasal sinuses, bronchioles of the lung, kidney, and around the vertebrae of the tail. Also seen in the olfactory bulb.1 Publication

Gene expression databases

BgeeiENSMUSG00000022246 Expressed in 267 organ(s), highest expression level in renal corpuscle
CleanExiMM_RAI14
GenevisibleiQ9EP71 MM

Interactioni

Subunit structurei

Interacts with PALLD (By similarity). Associates with actin (PubMed:11168586). However, does not bind F-actin directly (PubMed:11168586).By similarity1 Publication

Protein-protein interaction databases

BioGridi217644, 9 interactors
IntActiQ9EP71, 7 interactors
MINTiQ9EP71
STRINGi10090.ENSMUSP00000087815

Structurei

3D structure databases

ProteinModelPortaliQ9EP71
SMRiQ9EP71
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Repeati18 – 51ANK 1Add BLAST34
Repeati52 – 81ANK 2Add BLAST30
Repeati85 – 114ANK 3Add BLAST30
Repeati118 – 147ANK 4Add BLAST30
Repeati151 – 180ANK 5Add BLAST30
Repeati184 – 213ANK 6Add BLAST30
Repeati217 – 247ANK 7Add BLAST31

Coiled coil

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Coiled coili349 – 374Sequence analysisAdd BLAST26
Coiled coili430 – 943Sequence analysisAdd BLAST514

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi270 – 276Nuclear localization signalBy similarity7

Keywords - Domaini

ANK repeat, Coiled coil, Repeat

Phylogenomic databases

eggNOGiENOG410IEWD Eukaryota
COG0666 LUCA
GeneTreeiENSGT00530000063369
HOGENOMiHOG000154070
HOVERGENiHBG093886
InParanoidiQ9EP71
OMAiLLCEHKC
OrthoDBiEOG091G025R
PhylomeDBiQ9EP71
TreeFamiTF331274

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF12796 Ank_2, 2 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits

Sequence (1+)i

Sequence statusi: Complete.

This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All

Q9EP71-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSLKAKFRK SDTNEWNKND DRLLQAVENG DAEKVASLLG KKGASATKHD
60 70 80 90 100
SEGKTAFHLA AAKGHVECLK VMVTHGVDVT AQDSSGHSAL HVAAKNGHPE
110 120 130 140 150
CIRKLLQYKS PAENIDNSGK TALHYAAAQG CLQAVQLLCE HKSPINLKDL
160 170 180 190 200
DGNIPLLVAV QNGHSEACHF LLDHGADVNS RDKNGRTALM LACETGSSNT
210 220 230 240 250
VDALIKKGAD LSLVDSLGHN ALHYSKLSEN AGIQNLLLSK ISQDADLKTP
260 270 280 290 300
TKPKQHDQVS KISSERSGTP KKRKAPPPPI SPTQLSDVSS PRSITSTPLS
310 320 330 340 350
GKESVFFAEA PFKAEISSIQ ENKDRLSDST AGADSLLDIS SEADQQDLLV
360 370 380 390 400
LLQAKVASLT LHNKELQDKL QAKSPKDKEA EADLSFQSFH STQTDLAPSP
410 420 430 440 450
GKASDIPSSD AKSSPPVEHP AGTSTTDNDV IIRQLQDSLH DLQKRLESSE
460 470 480 490 500
AEKKQLQDEL QSQRTDTLCL NNTEISENGS DLSQKLKETQ SKYEEAMKEV
510 520 530 540 550
LSVQKQMKLG LLSQESADGY SHLREAPADE DIDTLKQDLQ KAVEESARNK
560 570 580 590 600
ERVRELETKL AEKEQAEATK PPAEACEELR SSYCSVIENM NKEKAFLFEK
610 620 630 640 650
YQQAQEEIMK LKDTLKSQMP QEAPDDSGDM KEAMNRMIDE LNKQVSELSQ
660 670 680 690 700
LYREAQAELE DYRKRKSLED AAEYIHKAEH ERLMHVSNLS RAKSEEALSE
710 720 730 740 750
MKSQYSKVLN ELTQLKQLVD AHKENSVSIT EHLQVITTLR TTAKEMEEKI
760 770 780 790 800
SALTGHLANK EAEVAKLEKQ LAEEKAAVSD AMVPKSSYEK LQASLESEVN
810 820 830 840 850
ALATKLKESV REREKAHSEV AQVRSEVSQA RREKDNIQTL LKAKEQEVTA
860 870 880 890 900
LVQKFQRAQE ELAGMRRCSE TSSKLEEDKD EKINEMTREV LKLKEALNSL
910 920 930 940 950
SQLSYSTSSS KRQSQQLDLL QQQVKQLQNQ LAECKKHHQE VISVYRMHLL
960 970
YAVQGQMDED VQKVLKQILT MCKNQSQKK
Length:979
Mass (Da):108,852
Last modified:March 1, 2001 - v1
Checksum:iB2E8C016D80237C4
GO

Computationally mapped potential isoform sequencesi

There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basket
EntryEntry nameProtein names
Gene namesLengthAnnotation
A0A2I3BPY7A0A2I3BPY7_MOUSE
Ankycorbin
Rai14
950Annotation score:

Sequence cautioni

The sequence BAC98143 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti420P → L in BAC98143 (PubMed:14621295).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202315 mRNA Translation: AAG24483.1
AF274866 mRNA Translation: AAG25937.1
AK129333 mRNA Translation: BAC98143.1 Different initiation.
BC052458 mRNA Translation: AAH52458.1
AK141233 mRNA Translation: BAE24605.1
CCDSiCCDS37043.1
RefSeqiNP_001159880.1, NM_001166408.1
NP_109615.1, NM_030690.3
XP_006520250.1, XM_006520187.2
XP_006520251.1, XM_006520188.3
XP_017172264.1, XM_017316775.1
UniGeneiMm.212395

Genome annotation databases

EnsembliENSMUST00000090339; ENSMUSP00000087815; ENSMUSG00000022246
ENSMUST00000169385; ENSMUSP00000126325; ENSMUSG00000022246
GeneIDi75646
KEGGimmu:75646
UCSCiuc007vgl.1 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AF202315 mRNA Translation: AAG24483.1
AF274866 mRNA Translation: AAG25937.1
AK129333 mRNA Translation: BAC98143.1 Different initiation.
BC052458 mRNA Translation: AAH52458.1
AK141233 mRNA Translation: BAE24605.1
CCDSiCCDS37043.1
RefSeqiNP_001159880.1, NM_001166408.1
NP_109615.1, NM_030690.3
XP_006520250.1, XM_006520187.2
XP_006520251.1, XM_006520188.3
XP_017172264.1, XM_017316775.1
UniGeneiMm.212395

3D structure databases

ProteinModelPortaliQ9EP71
SMRiQ9EP71
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi217644, 9 interactors
IntActiQ9EP71, 7 interactors
MINTiQ9EP71
STRINGi10090.ENSMUSP00000087815

PTM databases

iPTMnetiQ9EP71
PhosphoSitePlusiQ9EP71

Proteomic databases

EPDiQ9EP71
MaxQBiQ9EP71
PaxDbiQ9EP71
PeptideAtlasiQ9EP71
PRIDEiQ9EP71

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000090339; ENSMUSP00000087815; ENSMUSG00000022246
ENSMUST00000169385; ENSMUSP00000126325; ENSMUSG00000022246
GeneIDi75646
KEGGimmu:75646
UCSCiuc007vgl.1 mouse

Organism-specific databases

CTDi26064
MGIiMGI:1922896 Rai14
RougeiSearch...

Phylogenomic databases

eggNOGiENOG410IEWD Eukaryota
COG0666 LUCA
GeneTreeiENSGT00530000063369
HOGENOMiHOG000154070
HOVERGENiHBG093886
InParanoidiQ9EP71
OMAiLLCEHKC
OrthoDBiEOG091G025R
PhylomeDBiQ9EP71
TreeFamiTF331274

Miscellaneous databases

PROiPR:Q9EP71
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000022246 Expressed in 267 organ(s), highest expression level in renal corpuscle
CleanExiMM_RAI14
GenevisibleiQ9EP71 MM

Family and domain databases

CDDicd00204 ANK, 2 hits
Gene3Di1.25.40.20, 2 hits
InterProiView protein in InterPro
IPR002110 Ankyrin_rpt
IPR020683 Ankyrin_rpt-contain_dom
IPR036770 Ankyrin_rpt-contain_sf
PfamiView protein in Pfam
PF00023 Ank, 1 hit
PF12796 Ank_2, 2 hits
PRINTSiPR01415 ANKYRIN
SMARTiView protein in SMART
SM00248 ANK, 6 hits
SUPFAMiSSF48403 SSF48403, 1 hit
PROSITEiView protein in PROSITE
PS50297 ANK_REP_REGION, 1 hit
PS50088 ANK_REPEAT, 5 hits
ProtoNetiSearch...

Entry informationi

Entry nameiRAI14_MOUSE
AccessioniPrimary (citable) accession number: Q9EP71
Secondary accession number(s): Q3URT3, Q6ZPT6
Entry historyiIntegrated into UniProtKB/Swiss-Prot: June 13, 2006
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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