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Protein

Twisted gastrulation protein homolog 1

Gene

Twsg1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

May be involved in dorsoventral axis formation. Seems to antagonize BMP signaling by forming ternary complexes with CHRD and BMPs, thereby preventing BMPs from binding to their receptors. In addition to the anti-BMP function, also has pro-BMP activity, partly mediated by cleavage and degradation of CHRD, which releases BMPs from ternary complexes. May be an important modulator of BMP-regulated cartilage development and chondrocyte differentiation. May play a role in thymocyte development.4 Publications

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDevelopmental protein

Names & Taxonomyi

Protein namesi
Recommended name:
Twisted gastrulation protein homolog 1
Gene namesi
Name:Twsg1
Synonyms:Tsg
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:2137520 Twsg1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Secreted

Pathology & Biotechi

Disruption phenotypei

Embryonic lethality and sirenomelia were observed only in BMP null embryos in which one or two copies of TSG were also missing. When TSG and BMP7 are mutated, the siren phenotype results from the fusion of the limb buds in the ventroposterior midline owing to a paucity of posterior ventral mesoderm.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 24Sequence analysisAdd BLAST24
ChainiPRO_000027880925 – 222Twisted gastrulation protein homolog 1Add BLAST198

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Glycosylationi80N-linked (GlcNAc...) asparagineSequence analysis1
Glycosylationi146N-linked (GlcNAc...) asparagineSequence analysis1

Keywords - PTMi

Glycoprotein

Proteomic databases

EPDiQ9EP52
MaxQBiQ9EP52
PaxDbiQ9EP52
PeptideAtlasiQ9EP52
PRIDEiQ9EP52

PTM databases

iPTMnetiQ9EP52
PhosphoSitePlusiQ9EP52

Expressioni

Tissue specificityi

Expressed in lymph node, liver, kidney, and lung. Expression in the kidney was stronger in the medulla than in the cortex, particularly in the cells surrounding the medullary tubules. Expressed in growth plate cartilage of long bones, ribs, and digits and to a lesser extent also in the resting zone of the epiphysis, trabecular bone, and vertebral cartilage. Expression seems to be absent from other skeletal tissues including muscle, skin, and fibroblasts.1 Publication

Developmental stagei

Expressed at all embryonic stages examined. Expression was low and distribution was diffuse. At the primitive streak stage, detected in the extraembryonic region. Signal first appeared in the embryo during the neural plate stage. Stronger and more localized expression is seen after embryonic turning. Higher expression is seen in the branchial arch mesenchyme, the endoderm of the developing pharynx, all levels of the developing gut, the myotome compartment of the somites, and some regions of surface ectoderm. At 8.25 and 9.0 dpc expressed in head mesenchyme and ventral mesoderm.2 Publications

Gene expression databases

BgeeiENSMUSG00000024098 Expressed in 287 organ(s), highest expression level in embryo
CleanExiMM_TWSG1
GenevisibleiQ9EP52 MM

Interactioni

Subunit structurei

Interacts with CHRD and/or BMP4. This interaction enhances CHRD/BMP4 complex formation. Interacts with BMP7.3 Publications

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211157, 1 interactor
STRINGi10090.ENSMUSP00000024906

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi18 – 76Cys-richAdd BLAST59

Domaini

The N-terminal domain is sufficient to interact with BMP4.By similarity

Sequence similaritiesi

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiENOG410IF1M Eukaryota
ENOG4110NGY LUCA
GeneTreeiENSGT00390000007058
HOGENOMiHOG000044674
HOVERGENiHBG055753
InParanoidiQ9EP52
OMAiSCVDLCP
OrthoDBiEOG091G0J76
PhylomeDBiQ9EP52
TreeFamiTF323922

Family and domain databases

InterProiView protein in InterPro
IPR006761 Tsg
PANTHERiPTHR12312 PTHR12312, 1 hit
PfamiView protein in Pfam
PF04668 Tsg, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9EP52-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MKSHYIVLAL ASLTFLLCLP VSQSCNKALC ASDVSKCLIQ ELCQCRPGEG
60 70 80 90 100
NCPCCKECML CLGALWDECC DCVGMCNPRN YSDTPPTSKS TVEELHEPIP
110 120 130 140 150
SLFRALTEGD TQLNWNIVSF PVAEELSHHE NLVSFLETVN QLHHQNVSVP
160 170 180 190 200
SNNVHAPFPS DKERMCTVVY FDDCMSIHQC KISCESMGAS KYRWFHNACC
210 220
ECIGPECIDY GSKTVKCMNC MF
Length:222
Mass (Da):24,801
Last modified:March 1, 2001 - v1
Checksum:iF9B0CEE2A8DF8CA7
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti6I → IA in AAG10080 (Ref. 3) Curated1
Sequence conflicti75M → R in AAG10080 (Ref. 3) Curated1
Sequence conflicti75M → R in AAH04850 (PubMed:15489334).Curated1
Sequence conflicti168V → E in BAC28326 (PubMed:16141072).Curated1
Sequence conflicti203 – 204IG → MR in AAG10080 (Ref. 3) Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297390 mRNA Translation: CAC05586.1
AF292033 mRNA Translation: AAG00605.1
AF295097 mRNA Translation: AAG10080.1
AK018524 mRNA Translation: BAC25557.1
AK033504 mRNA Translation: BAC28326.1
AK049646 mRNA Translation: BAC33857.1
AK168547 mRNA Translation: BAE40422.1
AK168633 mRNA Translation: BAE40493.1
BC004850 mRNA Translation: AAH04850.1
CCDSiCCDS28942.1
RefSeqiNP_075540.1, NM_023053.3
UniGeneiMm.10153

Genome annotation databases

EnsembliENSMUST00000024906; ENSMUSP00000024906; ENSMUSG00000024098
GeneIDi65960
KEGGimmu:65960
UCSCiuc008dgq.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AJ297390 mRNA Translation: CAC05586.1
AF292033 mRNA Translation: AAG00605.1
AF295097 mRNA Translation: AAG10080.1
AK018524 mRNA Translation: BAC25557.1
AK033504 mRNA Translation: BAC28326.1
AK049646 mRNA Translation: BAC33857.1
AK168547 mRNA Translation: BAE40422.1
AK168633 mRNA Translation: BAE40493.1
BC004850 mRNA Translation: AAH04850.1
CCDSiCCDS28942.1
RefSeqiNP_075540.1, NM_023053.3
UniGeneiMm.10153

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211157, 1 interactor
STRINGi10090.ENSMUSP00000024906

PTM databases

iPTMnetiQ9EP52
PhosphoSitePlusiQ9EP52

Proteomic databases

EPDiQ9EP52
MaxQBiQ9EP52
PaxDbiQ9EP52
PeptideAtlasiQ9EP52
PRIDEiQ9EP52

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000024906; ENSMUSP00000024906; ENSMUSG00000024098
GeneIDi65960
KEGGimmu:65960
UCSCiuc008dgq.2 mouse

Organism-specific databases

CTDi57045
MGIiMGI:2137520 Twsg1

Phylogenomic databases

eggNOGiENOG410IF1M Eukaryota
ENOG4110NGY LUCA
GeneTreeiENSGT00390000007058
HOGENOMiHOG000044674
HOVERGENiHBG055753
InParanoidiQ9EP52
OMAiSCVDLCP
OrthoDBiEOG091G0J76
PhylomeDBiQ9EP52
TreeFamiTF323922

Miscellaneous databases

PROiPR:Q9EP52
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000024098 Expressed in 287 organ(s), highest expression level in embryo
CleanExiMM_TWSG1
GenevisibleiQ9EP52 MM

Family and domain databases

InterProiView protein in InterPro
IPR006761 Tsg
PANTHERiPTHR12312 PTHR12312, 1 hit
PfamiView protein in Pfam
PF04668 Tsg, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiTWSG1_MOUSE
AccessioniPrimary (citable) accession number: Q9EP52
Secondary accession number(s): Q8CCB1
, Q8CEM6, Q99K77, Q9ERN7
Entry historyiIntegrated into UniProtKB/Swiss-Prot: March 6, 2007
Last sequence update: March 1, 2001
Last modified: November 7, 2018
This is version 112 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
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Main funding by: National Institutes of Health

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