UniProtKB - Q9DEX7 (FADS2_DANRE)
Protein
Acyl-CoA 6-desaturase
Gene
fads2
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Fatty acid desaturase with bifunctional delta-5 and delta-6 activities. Component of a lipid metabolic pathway that catalyzes the biosynthesis of polyunsaturated fatty acids (PUFA) with preference toward n-3 substrates and Delta-6 function.1 Publication
Catalytic activityi
- (9Z,12Z)-octadecadienoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H+ + O2 = (6Z,9Z,12Z)-octadecatrienoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.31 PublicationThis reaction proceeds in the forward1 Publication direction.
- (9Z,12Z,15Z)-octadecatrienoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H+ + O2 = (6Z,9Z,12Z,15Z)-octadecatetraenoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.31 PublicationThis reaction proceeds in the forward1 Publication direction.
- (8Z,11Z,14Z,17Z)-eicosatetraenoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H+ + O2 = (5Z,8Z,11Z,14Z,17Z)-eicosapentaenoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.441 PublicationThis reaction proceeds in the forward1 Publication direction.
- (8Z,11Z,14Z)-eicosatrienoyl-CoA + 2 [Fe(II)-cytochrome b5] + 2 H+ + O2 = (5Z,8Z,11Z,14Z)-eicosatetraenoyl-CoA + 2 [Fe(III)-cytochrome b5] + 2 H2O1 PublicationEC:1.14.19.441 PublicationThis reaction proceeds in the forward1 Publication direction.
: polyunsaturated fatty acid biosynthesis Pathwayi
This protein is involved in the pathway polyunsaturated fatty acid biosynthesis, which is part of Lipid metabolism.1 PublicationView all proteins of this organism that are known to be involved in the pathway polyunsaturated fatty acid biosynthesis and in Lipid metabolism.
GO - Molecular functioni
- acyl-CoA delta5-desaturase activity Source: UniProtKB-EC
- linoleoyl-CoA desaturase activity Source: UniProtKB-EC
GO - Biological processi
- liver development Source: ZFIN
- unsaturated fatty acid biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Oxidoreductase |
Biological process | Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport |
Enzyme and pathway databases
BRENDAi | 1.14.19.3, 928 |
Reactomei | R-DRE-2046105, Linoleic acid (LA) metabolism R-DRE-2046106, alpha-linolenic acid (ALA) metabolism |
UniPathwayi | UPA00658 |
Chemistry databases
SwissLipidsi | SLP:000000464 |
Names & Taxonomyi
Protein namesi | Recommended name: Acyl-CoA 6-desaturaseBy similarity (EC:1.14.19.31 Publication, EC:1.14.19.441 Publication)Alternative name(s): Delta(5)/Delta(6) fatty acid desaturase Short name: D5D/D6D fatty acid desaturase Short name: Delta-5/Delta-6 fatty acid desaturase Fatty acid desaturase 2 |
Gene namesi | Name:fads2 Synonyms:fadsd6 |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-011212-1, fads2 |
Subcellular locationi
Endoplasmic reticulum
- Endoplasmic reticulum membrane Curated; Multi-pass membrane protein Curated
Endoplasmic reticulum
- endoplasmic reticulum membrane Source: UniProtKB-SubCell
Other locations
- integral component of membrane Source: UniProtKB-KW
Topology
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Topological domaini | 1 – 130 | CytoplasmicCuratedAdd BLAST | 130 | |
Transmembranei | 131 – 151 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 152 – 157 | LumenalCurated | 6 | |
Transmembranei | 158 – 178 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 179 – 264 | CytoplasmicCuratedAdd BLAST | 86 | |
Transmembranei | 265 – 285 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 286 – 305 | LumenalCuratedAdd BLAST | 20 | |
Transmembranei | 306 – 326 | HelicalSequence analysisAdd BLAST | 21 | |
Topological domaini | 327 – 444 | CytoplasmicCuratedAdd BLAST | 118 |
Keywords - Cellular componenti
Endoplasmic reticulum, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000185408 | 1 – 444 | Acyl-CoA 6-desaturaseAdd BLAST | 444 |
Proteomic databases
PaxDbi | Q9DEX7 |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 18 – 95 | Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST | 78 |
Motif
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Motifi | 180 – 184 | Histidine box-1 | 5 | |
Motifi | 217 – 221 | Histidine box-2 | 5 | |
Motifi | 382 – 386 | Histidine box-3 | 5 |
Domaini
The protein sequence includes a number of characteristic features of microsomal fatty acid desaturases including the three histidine boxes HXXXH, HXXHH, and QXXHH (these domains may contain the active site and/or be involved in metal ion binding), and the N-terminal cytochrome b5 domain containing the heme-binding motif, HPGG, similar to that of other fatty acid desaturases.1 Publication
Sequence similaritiesi
Belongs to the fatty acid desaturase type 1 family.Curated
Keywords - Domaini
Transmembrane, Transmembrane helixPhylogenomic databases
eggNOGi | KOG4232, Eukaryota |
InParanoidi | Q9DEX7 |
OrthoDBi | 1060606at2759 |
PhylomeDBi | Q9DEX7 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR005804, FA_desaturase_dom IPR012171, Fatty_acid_desaturase |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF015921, FA_sphinglp_des, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS50255, CYTOCHROME_B5_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9DEX7-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MGGGGQQTDR ITDTNGRFSS YTWEEVQKHT KHGDQWVVVE RKVYNVSQWV
60 70 80 90 100
KRHPGGLRIL GHYAGEDATE AFTAFHPNLQ LVRKYLKPLL IGELEASEPS
110 120 130 140 150
QDRQKNAALV EDFRALRERL EAEGCFKTQP LFFALHLGHI LLLEAIAFMM
160 170 180 190 200
VWYFGTGWIN TLIVAVILAT AQSQAGWLQH DFGHLSVFKT SGMNHLVHKF
210 220 230 240 250
VIGHLKGASA GWWNHRHFQH HAKPNIFKKD PDVNMLNAFV VGNVQPVEYG
260 270 280 290 300
VKKIKHLPYN HQHKYFFFIG PPLLIPVYFQ FQIFHNMISH GMWVDLLWCI
310 320 330 340 350
SYYVRYFLCY TQFYGVFWAI ILFNFVRFME SHWFVWVTQM SHIPMNIDYE
360 370 380 390 400
KNQDWLSMQL VATCNIEQSA FNDWFSGHLN FQIEHHLFPT VPRHNYWRAA
410 420 430 440
PRVRALCEKY GVKYQEKTLY GAFADIIRSL EKSGELWLDA YLNK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketF1R3G9 | F1R3G9_DANRE | Acyl-CoA 6-desaturase | fads2 | 444 | Annotation score: | ||
F6NP64 | F6NP64_DANRE | Acyl-CoA 6-desaturase | fads2 | 421 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 26 | V → M in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 82 | V → M in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 149 | M → V in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 342 | H → R in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 351 | K → Q in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 391 | V → M in AAH49438 (Ref. 2) Curated | 1 | |
Sequence conflicti | 405 | A → S in AAH49438 (Ref. 2) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF309556 mRNA Translation: AAG25710.1 BC049438 mRNA Translation: AAH49438.1 |
RefSeqi | NP_571720.2, NM_131645.2 |
Genome annotation databases
GeneIDi | 140615 |
KEGGi | dre:140615 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF309556 mRNA Translation: AAG25710.1 BC049438 mRNA Translation: AAH49438.1 |
RefSeqi | NP_571720.2, NM_131645.2 |
3D structure databases
SMRi | Q9DEX7 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000022396 |
Chemistry databases
SwissLipidsi | SLP:000000464 |
Proteomic databases
PaxDbi | Q9DEX7 |
Genome annotation databases
GeneIDi | 140615 |
KEGGi | dre:140615 |
Organism-specific databases
CTDi | 9415 |
ZFINi | ZDB-GENE-011212-1, fads2 |
Phylogenomic databases
eggNOGi | KOG4232, Eukaryota |
InParanoidi | Q9DEX7 |
OrthoDBi | 1060606at2759 |
PhylomeDBi | Q9DEX7 |
Enzyme and pathway databases
UniPathwayi | UPA00658 |
BRENDAi | 1.14.19.3, 928 |
Reactomei | R-DRE-2046105, Linoleic acid (LA) metabolism R-DRE-2046106, alpha-linolenic acid (ALA) metabolism |
Miscellaneous databases
ChiTaRSi | fads2, zebrafish |
PROi | PR:Q9DEX7 |
Family and domain databases
Gene3Di | 3.10.120.10, 1 hit |
InterProi | View protein in InterPro IPR001199, Cyt_B5-like_heme/steroid-bd IPR036400, Cyt_B5-like_heme/steroid_sf IPR005804, FA_desaturase_dom IPR012171, Fatty_acid_desaturase |
Pfami | View protein in Pfam PF00173, Cyt-b5, 1 hit PF00487, FA_desaturase, 1 hit |
PIRSFi | PIRSF015921, FA_sphinglp_des, 1 hit |
PRINTSi | PR00363, CYTOCHROMEB5 |
SMARTi | View protein in SMART SM01117, Cyt-b5, 1 hit |
SUPFAMi | SSF55856, SSF55856, 1 hit |
PROSITEi | View protein in PROSITE PS50255, CYTOCHROME_B5_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FADS2_DANRE | |
Accessioni | Q9DEX7Primary (citable) accession number: Q9DEX7 Secondary accession number(s): Q7ZWF3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | October 19, 2002 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 122 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families