UniProtKB - Q9DEX7 (FADS2_DANRE)

BLAST

>sp|Q9DEX7|FADS2_DANRE Fatty acid desaturase 2 OS=Danio rerio OX=7955 GN=fads2 PE=2 SV=1
MGGGGQQTDRITDTNGRFSSYTWEEVQKHTKHGDQWVVVERKVYNVSQWVKRHPGGLRIL
GHYAGEDATEAFTAFHPNLQLVRKYLKPLLIGELEASEPSQDRQKNAALVEDFRALRERL
EAEGCFKTQPLFFALHLGHILLLEAIAFMMVWYFGTGWINTLIVAVILATAQSQAGWLQH
DFGHLSVFKTSGMNHLVHKFVIGHLKGASAGWWNHRHFQHHAKPNIFKKDPDVNMLNAFV
VGNVQPVEYGVKKIKHLPYNHQHKYFFFIGPPLLIPVYFQFQIFHNMISHGMWVDLLWCI
SYYVRYFLCYTQFYGVFWAIILFNFVRFMESHWFVWVTQMSHIPMNIDYEKNQDWLSMQL
VATCNIEQSAFNDWFSGHLNFQIEHHLFPTVPRHNYWRAAPRVRALCEKYGVKYQEKTLY
GAFADIIRSLEKSGELWLDAYLNK

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History
Entry version 113 (23 May 2018)
Sequence version 1 (01 Mar 2001)
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Protein

Fatty acid desaturase 2

Gene

fads2

Organism

Danio rerio (Zebrafish) (Brachydanio rerio)

Status

Reviewed-Annotation score: -Experimental evidence at transcript levelⁱ

Functionⁱ

Fatty acid desaturase with both Delta₅ and Delta₆ activities. Component of a lipid metabolic pathway that catalyzes biosynthesis of highly unsaturated fatty acids (HUFA) from precursor essential polyunsaturated fatty acids (PUFA) linoleic acid (LA) (18:2n-6) and alpha-linolenic acid (ALA) (18:3n-3).By similarity

Catalytic activityⁱ

Linoleoyl-CoA + 2 ferrocytochrome b5 + O₂ + 2 H⁺ = gamma-linolenoyl-CoA + 2 ferricytochrome b5 + 2 H₂O.By similarity

Alpha-linolenoyl-CoA + 2 ferrocytochrome b5 + O₂ + 2 H⁺ = stearidonoyl-CoA + 2 ferricytochrome b5 + 2 H₂O.By similarity

Pathwayⁱ: polyunsaturated fatty acid biosynthesis

This protein is involved in the pathway polyunsaturated fatty acid biosynthesis, which is part of Lipid metabolism.
View all proteins of this organism that are known to be involved in the pathway polyunsaturated fatty acid biosynthesis and in Lipid metabolism.

GO - Molecular functionⁱ

linoleoyl-CoA desaturase activity Source: UniProtKB-EC

View the complete GO annotation on QuickGO ...

GO - Biological processⁱ

liver development
Source: ZFIN
Inferred from mutant phenotypeⁱ
- 23813869
unsaturated fatty acid biosynthetic process Source: UniProtKB-UniPathway

View the complete GO annotation on QuickGO ...

Keywordsⁱ

Molecular function	Oxidoreductase
Biological process	Electron transport, Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism, Transport

Enzyme and pathway databases

BRENDAⁱ	1.14.19.3 928
UniPathwayⁱ	UPA00658

Chemistry databases

SwissLipidsⁱ	SLP:000000464

SwissLipidsⁱ

SLP:000000464

Names & Taxonomyⁱ

Protein namesⁱ	Recommended name: Fatty acid desaturase 2 (EC:1.14.19.3) Alternative name(s): Acyl-CoA 6-desaturase Delta(5)/Delta(6) fatty acid desaturase Short name: D5D/D6D fatty acid desaturase Short name: Delta-5/Delta-6 fatty acid desaturase
Gene namesⁱ	Name:fads2 Synonyms:fadsd6
Organismⁱ	Danio rerio (Zebrafish) (Brachydanio rerio)
Taxonomic identifierⁱ	7955 [NCBI]
Taxonomic lineageⁱ	cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Actinopterygii › Actinopteri › Neopterygii › Teleostei › Osteoglossocephalai › Clupeocephala › Otomorpha › Ostariophysi › Otophysi › Cypriniphysae › Cypriniformes › Cyprinoidea › Cyprinidae › Danio
Proteomesⁱ	UP000000437 Componentⁱ: Unplaced

Organism-specific databases

ZFINⁱ	ZDB-GENE-011212-1 fads2

ZFINⁱ

ZDB-GENE-011212-1 fads2

Topology

Feature key	Position(s)	DescriptionActions	Length
Topological domainⁱ	1 – 130	CytoplasmicSequence analysisAdd BLAST	130
Transmembraneⁱ	131 – 151	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	152 – 157	LumenalSequence analysis	6
Transmembraneⁱ	158 – 178	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	179 – 264	CytoplasmicSequence analysisAdd BLAST	86
Transmembraneⁱ	265 – 285	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	286 – 305	LumenalSequence analysisAdd BLAST	20
Transmembraneⁱ	306 – 326	HelicalSequence analysisAdd BLAST	21
Topological domainⁱ	327 – 444	CytoplasmicSequence analysisAdd BLAST	118

Keywords - Cellular componentⁱ

Endoplasmic reticulum, Membrane

PTM / Processingⁱ

Molecule processing

Feature key	Position(s)	DescriptionActions	Graphical view	Length
ChainⁱPRO_0000185408	1 – 444	Fatty acid desaturase 2Add BLAST		444

Proteomic databases

PaxDbⁱ	Q9DEX7

PaxDbⁱ

Q9DEX7

Interactionⁱ

Protein-protein interaction databases

STRINGⁱ	7955.ENSDARP00000022396

STRINGⁱ

7955.ENSDARP00000022396

Structureⁱ

3D structure databases

ProteinModelPortalⁱ	Q9DEX7
SMRⁱ	Q9DEX7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Family & Domainsⁱ

Domains and Repeats

Feature key	Position(s)	DescriptionActions	Graphical view	Length
Domainⁱ	18 – 95	Cytochrome b5 heme-bindingPROSITE-ProRule annotationAdd BLAST		78

Motif

Feature key	Position(s)	DescriptionActions	Length
Motifⁱ	180 – 184	Histidine box-1	5
Motifⁱ	217 – 221	Histidine box-2	5
Motifⁱ	382 – 386	Histidine box-3	5

Domainⁱ

The histidine box domains may contain the active site and/or be involved in metal ion binding.

Sequence similaritiesⁱ

Belongs to the fatty acid desaturase type 1 family.Curated

Keywords - Domainⁱ

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGⁱ	KOG4232 Eukaryota ENOG410XVSZ LUCA
HOGENOMⁱ	HOG000012997
HOVERGENⁱ	HBG002839
InParanoidⁱ	Q9DEX7
KEGG Orthology (KO) More...KOⁱ	K10226
PhylomeDBⁱ	Q9DEX7

Family and domain databases

Gene3Dⁱ	3.10.120.10, 1 hit
InterProⁱ	View protein in InterPro IPR001199 Cyt_B5-like_heme/steroid-bd IPR036400 Cyt_B5-like_heme/steroid_sf IPR005804 FA_desaturase_dom IPR012171 Fatty_acid_desaturase
Pfam protein domain database More...Pfamⁱ	View protein in Pfam PF00173 Cyt-b5, 1 hit PF00487 FA_desaturase, 1 hit
PIRSFⁱ	PIRSF015921 FA_sphinglp_des, 1 hit
PRINTSⁱ	PR00363 CYTOCHROMEB5
SMARTⁱ	View protein in SMART SM01117 Cyt-b5, 1 hit
SUPFAMⁱ	SSF55856 SSF55856, 1 hit
PROSITEⁱ	View protein in PROSITE PS50255 CYTOCHROME_B5_2, 1 hit

Sequence (1+)ⁱ

Sequence statusⁱ: Complete.

This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.ⁱShow all

Q9DEX7-1 [UniParc]FASTA Add to basket

« Hide

        10         20         30         40         50
MGGGGQQTDR ITDTNGRFSS YTWEEVQKHT KHGDQWVVVE RKVYNVSQWV 
        60         70         80         90        100
KRHPGGLRIL GHYAGEDATE AFTAFHPNLQ LVRKYLKPLL IGELEASEPS 
       110        120        130        140        150
QDRQKNAALV EDFRALRERL EAEGCFKTQP LFFALHLGHI LLLEAIAFMM 
       160        170        180        190        200
VWYFGTGWIN TLIVAVILAT AQSQAGWLQH DFGHLSVFKT SGMNHLVHKF 
       210        220        230        240        250
VIGHLKGASA GWWNHRHFQH HAKPNIFKKD PDVNMLNAFV VGNVQPVEYG 
       260        270        280        290        300
VKKIKHLPYN HQHKYFFFIG PPLLIPVYFQ FQIFHNMISH GMWVDLLWCI 
       310        320        330        340        350
SYYVRYFLCY TQFYGVFWAI ILFNFVRFME SHWFVWVTQM SHIPMNIDYE 
       360        370        380        390        400
KNQDWLSMQL VATCNIEQSA FNDWFSGHLN FQIEHHLFPT VPRHNYWRAA 
       410        420        430        440 
PRVRALCEKY GVKYQEKTLY GAFADIIRSL EKSGELWLDA YLNK

Length:444

Mass (Da):52,032

Last modified:March 1, 2001 - v1

Checksum:ⁱ6AA25A1DC1DC0F65

Computationally mapped potential isoform sequencesⁱ

There are 2 potential isoforms mapped to this entry.BLAST Align Show all Add to basket

	Entry	Entry name		Protein names	Gene names	Length	Annotation
	F1R3G9	F1R3G9_DANRE		Fatty acid desaturase 2 Fatty acid desaturase 2	fads2	444	Annotation score:
	F6NP64	F6NP64_DANRE		Fatty acid desaturase 2 Fatty acid desaturase 2	fads2	421	Annotation score:

Experimental Info

Feature key	Position(s)	DescriptionActions	Length
Sequence conflictⁱ	26	V → M in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	82	V → M in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	149	M → V in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	342	H → R in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	351	K → Q in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	391	V → M in AAH49438 (Ref. 2) Curated	1
Sequence conflictⁱ	405	A → S in AAH49438 (Ref. 2) Curated	1

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF309556 mRNA Translation: AAG25710.1 BC049438 mRNA Translation: AAH49438.1
NCBI Reference Sequences More...RefSeqⁱ	NP_571720.2, NM_131645.2
UniGeneⁱ	Dr.75661

Genome annotation databases

GeneIDⁱ	140615
KEGGⁱ	dre:140615

Similar proteinsⁱ

90% Identity
50% Identity

Protein	Similar proteins		Species	Score	Length	Source
Q9DEX7	Fatty acid desaturase 2		DANRE		444	UniRef90_Q9DEX7
Delta-6 fatty acyl desaturase		LABRO		444
Delta-6 fatty acid desaturase (Fragment)		CARGB		359
Fads2 protein		DANRE		158
UPI000017D952		DANRE		444

Protein	Similar proteins		Species	Score	Length	Source
Q9DEX7	Fatty acid desaturase 2		HUMAN		444	UniRef50_O95864
Fatty acid desaturase 2		PONAB		444
Fatty acid desaturase 2		MACFA		444
Fatty acid desaturase 2		XENLA		446
Fatty acid desaturase 3		MOUSE		449
+362

Cross-referencesⁱ

Sequence databases

Select the link destinations: EMBLⁱ GenBankⁱ DDBJⁱ Links Updated	AF309556 mRNA Translation: AAG25710.1 BC049438 mRNA Translation: AAH49438.1
RefSeqⁱ	NP_571720.2, NM_131645.2
UniGeneⁱ	Dr.75661

3D structure databases

ProteinModelPortalⁱ	Q9DEX7
SMRⁱ	Q9DEX7
ModBaseⁱ	Search...
MobiDBⁱ	Search...

Protein-protein interaction databases

STRINGⁱ	7955.ENSDARP00000022396

STRINGⁱ

7955.ENSDARP00000022396

Chemistry databases

SwissLipidsⁱ	SLP:000000464

SwissLipidsⁱ

SLP:000000464

Proteomic databases

PaxDbⁱ	Q9DEX7

PaxDbⁱ

Q9DEX7

Protocols and materials databases

Structural Biology Knowledgebase	Search...

Structural Biology Knowledgebase

Search...

Genome annotation databases

GeneIDⁱ	140615
KEGGⁱ	dre:140615

Organism-specific databases

CTDⁱ	9415
ZFINⁱ	ZDB-GENE-011212-1 fads2

Phylogenomic databases

eggNOGⁱ	KOG4232 Eukaryota ENOG410XVSZ LUCA
HOGENOMⁱ	HOG000012997
HOVERGENⁱ	HBG002839
InParanoidⁱ	Q9DEX7
KOⁱ	K10226
PhylomeDBⁱ	Q9DEX7

Enzyme and pathway databases

UniPathwayⁱ	UPA00658
BRENDAⁱ	1.14.19.3 928

Miscellaneous databases

ChiTaRSⁱ	fads2 zebrafish
Protein Ontology More...PROⁱ	PR:Q9DEX7

Family and domain databases

Gene3Dⁱ	3.10.120.10, 1 hit
InterProⁱ	View protein in InterPro IPR001199 Cyt_B5-like_heme/steroid-bd IPR036400 Cyt_B5-like_heme/steroid_sf IPR005804 FA_desaturase_dom IPR012171 Fatty_acid_desaturase
Pfamⁱ	View protein in Pfam PF00173 Cyt-b5, 1 hit PF00487 FA_desaturase, 1 hit
PIRSFⁱ	PIRSF015921 FA_sphinglp_des, 1 hit
PRINTSⁱ	PR00363 CYTOCHROMEB5
SMARTⁱ	View protein in SMART SM01117 Cyt-b5, 1 hit
SUPFAMⁱ	SSF55856 SSF55856, 1 hit
PROSITEⁱ	View protein in PROSITE PS50255 CYTOCHROME_B5_2, 1 hit
ProtoNetⁱ	Search...

Entry informationⁱ

Entry nameⁱ	FADS2_DANRE
Accessionⁱ	Q9DEX7Primary (citable) accession number: Q9DEX7 Secondary accession number(s): Q7ZWF3
Entry historyⁱ	Integrated into UniProtKB/Swiss-Prot:	October 19, 2002
	Last sequence update:	March 1, 2001
	Last modified:	May 23, 2018
	This is version 113 of the entry and version 1 of the sequence. See complete history.
Entry statusⁱ	Reviewed (UniProtKB/Swiss-Prot)
Annotation program	Chordata Protein Annotation Program

Miscellaneousⁱ

Keywords - Technical termⁱ

Complete proteome, Reference proteome

UniProtKB

UniParc

Help

UniRef

Proteomes

Annotation systems

Supporting data

UniProtKB - Q9DEX7 (FADS2_DANRE)

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Fatty acid desaturase 2

fads2

Select a section on the left to see content.

<p>This section provides any useful information about the protein, mostly biological knowledge.<p><a href='/help/function_section' target='_top'>More...</a></p>Functioni

<p>This subsection of the <a href="http://www.uniprot.org/help/function_section">'Function'</a> section describes the metabolic pathway(s) associated with a protein.<p><a href='/help/pathway' target='_top'>More...</a></p>Pathwayi: polyunsaturated fatty acid biosynthesis

<p>The <a href="http://www.geneontology.org/">Gene Ontology (GO)</a> project provides a set of hierarchical controlled vocabulary split into 3 categories:<p><a href='/help/gene_ontology' target='_top'>More...</a></p>GO - Molecular functioni

GO - Biological processi

Enzyme and pathway databases

Chemistry databases

<p>This section provides information about the protein and gene name(s) and synonym(s) and about the organism that is the source of the protein sequence.<p><a href='/help/names_and_taxonomy_section' target='_top'>More...</a></p>Names & Taxonomyi

Organism-specific databases

<p>This section provides information on the location and the topology of the mature protein in the cell.<p><a href='/help/subcellular_location_section' target='_top'>More...</a></p>Subcellular locationi

Endoplasmic reticulum

Endoplasmic reticulum

Other locations

Topology

Keywords - Cellular componenti

<p>This section describes post-translational modifications (PTMs) and/or processing events.<p><a href='/help/ptm_processing_section' target='_top'>More...</a></p>PTM / Processingi

Molecule processing

Proteomic databases

<p>This section provides information on the quaternary structure of a protein and on interaction(s) with other proteins or protein complexes.<p><a href='/help/interaction_section' target='_top'>More...</a></p>Interactioni

Protein-protein interaction databases

<p>This section provides information on the tertiary and secondary structure of a protein.<p><a href='/help/structure_section' target='_top'>More...</a></p>Structurei

3D structure databases

<p>This section provides information on sequence similarities with other proteins and the domain(s) present in a protein.<p><a href='/help/family_and_domains_section' target='_top'>More...</a></p>Family & Domainsi

Domains and Repeats

Motif

<p>This subsection of the ‘Family and domains’ section provides information about the sequence similarity with other proteins.<p><a href='/help/sequence_similarities' target='_top'>More...</a></p>Sequence similaritiesi

Keywords - Domaini

Phylogenomic databases

Family and domain databases

Computationally mapped potential isoform sequencesi

Experimental Info

Sequence databases

Genome annotation databases

<p>This section is used to point to information related to entries and found in data collections other than UniProtKB.<p><a href='/help/cross_references_section' target='_top'>More...</a></p>Cross-referencesi

Sequence databases

3D structure databases

Protein-protein interaction databases

Chemistry databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Miscellaneous databases

Family and domain databases

<p>This section provides general information on the entry.<p><a href='/help/entry_information_section' target='_top'>More...</a></p>Entry informationi

<p>This section contains any relevant information that doesn’t fit in any other defined sections<p><a href='/help/miscellaneous_section' target='_top'>More...</a></p>Miscellaneousi

Keywords - Technical termi

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Functionⁱ

Pathwayⁱ: polyunsaturated fatty acid biosynthesis

GO - Molecular functionⁱ

GO - Biological processⁱ

Names & Taxonomyⁱ

Subcellular locationⁱ

Keywords - Cellular componentⁱ

PTM / Processingⁱ

Interactionⁱ

Structureⁱ

Family & Domainsⁱ

Sequence similaritiesⁱ

Keywords - Domainⁱ

Computationally mapped potential isoform sequencesⁱ

Cross-referencesⁱ

Entry informationⁱ

Miscellaneousⁱ

Keywords - Technical termⁱ