UniProtKB - Q9DDE3 (ACES_DANRE)
Protein
Acetylcholinesterase
Gene
ache
Organism
Danio rerio (Zebrafish) (Brachydanio rerio)
Status
Functioni
Terminates signal transduction at the neuromuscular junction by rapid hydrolysis of the acetylcholine released into the synaptic cleft.
Miscellaneous
No other isoforms exist. This protein corresponds to the T isoform in other species.
Catalytic activityi
- EC:3.1.1.7
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 225 | Acyl-ester intermediatePROSITE-ProRule annotation | 1 | |
Active sitei | 352 | Charge relay systemBy similarity | 1 | |
Active sitei | 495 | Charge relay systemBy similarity | 1 |
GO - Molecular functioni
- acetylcholinesterase activity Source: ZFIN
GO - Biological processi
- acetylcholine catabolic process Source: ZFIN
- cellular response to methanol Source: ZFIN
- myofibril assembly Source: ZFIN
- neuromuscular junction development Source: ZFIN
- neuron development Source: ZFIN
- regulation of synapse structure or activity Source: ZFIN
- response to amphetamine Source: ZFIN
- response to ethanol Source: ZFIN
- response to glucose Source: ZFIN
Keywordsi
Molecular function | Hydrolase, Serine esterase |
Biological process | Neurotransmitter degradation |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 928 |
Protein family/group databases
ESTHERi | danre-ACHE, AChE |
MEROPSi | S09.980 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:ache |
Organismi | Danio rerio (Zebrafish) (Brachydanio rerio) |
Taxonomic identifieri | 7955 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Actinopterygii › Neopterygii › Teleostei › Ostariophysi › Cypriniformes › Danionidae › Danioninae › Danio |
Proteomesi |
|
Organism-specific databases
ZFINi | ZDB-GENE-010906-1, ache |
Subcellular locationi
Plasma membrane
- Cell membrane By similarity; Peripheral membrane protein By similarity
Extracellular region or secreted
Other locations
Extracellular region or secreted
- extracellular region Source: UniProtKB-SubCell
Plasma Membrane
- plasma membrane Source: UniProtKB-SubCell
Other locations
- synapse Source: UniProtKB-SubCell
Keywords - Cellular componenti
Cell junction, Cell membrane, Membrane, Secreted, SynapsePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Signal peptidei | 1 – 23 | Sequence analysisAdd BLAST | 23 | |
ChainiPRO_0000008593 | 24 – 634 | AcetylcholinesteraseAdd BLAST | 611 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Disulfide bondi | 91 ↔ 118 | By similarity | ||
Glycosylationi | 133 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 184 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 279 ↔ 290 | By similarity | ||
Glycosylationi | 283 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 368 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 427 ↔ 580 | By similarity | ||
Glycosylationi | 512 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Glycosylationi | 592 | N-linked (GlcNAc...) asparagineSequence analysis | 1 | |
Disulfide bondi | 631 | InterchainBy similarity |
Keywords - PTMi
Disulfide bond, GlycoproteinProteomic databases
PaxDbi | Q9DDE3 |
Expressioni
Gene expression databases
Bgeei | ENSDARG00000031796, Expressed in muscle organ and 32 other tissues |
ExpressionAtlasi | Q9DDE3, baseline |
Interactioni
Subunit structurei
Dimers and collagen-tailed forms, in which catalytic tetramers are associated with anchoring proteins that attach them to the basal lamina or to cell membranes. In the collagen-tailed forms, subunits are associated with a specific collagen, COLQ, which triggers the formation of isoform T tetramers from dimers.
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000052988 |
Family & Domainsi
Sequence similaritiesi
Belongs to the type-B carboxylesterase/lipase family.Curated
Keywords - Domaini
SignalPhylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000157637 |
HOGENOMi | CLU_006586_13_0_1 |
InParanoidi | Q9DDE3 |
OMAi | PWCPLHT |
OrthoDBi | 754103at2759 |
PhylomeDBi | Q9DDE3 |
TreeFami | TF315470 |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
Q9DDE3-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MKTSDILLLP TVLLTFLFHN CFAQAEPDLV VATRLGRVQG TRLPVPDRSH
60 70 80 90 100
VIAFLGIPYA EPPIGKRRFK RAEPKKPWNN VFEAKEFSNA CYQFVDTSYP
110 120 130 140 150
GFPGIEMWNP NRVMSEDCLY LNVWVPPTPR PQNLTVMVWI YGGGFYSGSS
160 170 180 190 200
SLDVYDGRYL AYTEKVVVVS MNYRVGAFGF LALNGSSDAP GNVGLYDQRL
210 220 230 240 250
ALQWVQENIH FFGGNPKQVT IFGESAGAAS VGMHVLSPDS RPLFTRAILQ
260 270 280 290 300
SGVPNTPWAT VTFDEARRRT TKLGKLVGCT WGNDTELIDC LRNKHPQELI
310 320 330 340 350
DQEWQVLPWS SLFRFSFVPV VDGVFFPDTP DAMISSGNFK YTQILLGVNQ
360 370 380 390 400
DEGSYFLLYG APGFSKDNES LISREDFLES VKMGVPHAND IGLEAVILQY
410 420 430 440 450
TDWMDENNGQ KNRDAMDDIV GDQNVICPLQ HFAKSYAQYA ALHAQSSAAA
460 470 480 490 500
PGTLGWGNSG PTGYNSGNSH GAVYLYLFDH RASNLAWPEW MGVIHGYEIE
510 520 530 540 550
FVFGLPLEKR LNYTAEEEKL SRRIMRYWAN FARTGNPNVN TDGTMDSRRR
560 570 580 590 600
WPQFSANEQK HVGLNTEPMK VHKGLRTQFC ALWNRFLPRL LNITDNIDDV
610 620 630
ERQWKVEFHR WSSYMMHWKS QFDHYSKQER CTDL
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ251640 Genomic DNA Translation: CAC19790.1 |
RefSeqi | NP_571921.1, NM_131846.2 |
Genome annotation databases
Ensembli | ENSDART00000052989; ENSDARP00000052988; ENSDARG00000031796 |
GeneIDi | 114549 |
KEGGi | dre:114549 |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AJ251640 Genomic DNA Translation: CAC19790.1 |
RefSeqi | NP_571921.1, NM_131846.2 |
3D structure databases
SMRi | Q9DDE3 |
ModBasei | Search... |
Protein-protein interaction databases
STRINGi | 7955.ENSDARP00000052988 |
Chemistry databases
ChEMBLi | CHEMBL3308995 |
Protein family/group databases
ESTHERi | danre-ACHE, AChE |
MEROPSi | S09.980 |
Proteomic databases
PaxDbi | Q9DDE3 |
Genome annotation databases
Ensembli | ENSDART00000052989; ENSDARP00000052988; ENSDARG00000031796 |
GeneIDi | 114549 |
KEGGi | dre:114549 |
Organism-specific databases
CTDi | 43 |
ZFINi | ZDB-GENE-010906-1, ache |
Phylogenomic databases
eggNOGi | KOG4389, Eukaryota |
GeneTreei | ENSGT00940000157637 |
HOGENOMi | CLU_006586_13_0_1 |
InParanoidi | Q9DDE3 |
OMAi | PWCPLHT |
OrthoDBi | 754103at2759 |
PhylomeDBi | Q9DDE3 |
TreeFami | TF315470 |
Enzyme and pathway databases
BRENDAi | 3.1.1.7, 928 |
Miscellaneous databases
PROi | PR:Q9DDE3 |
Gene expression databases
Bgeei | ENSDARG00000031796, Expressed in muscle organ and 32 other tissues |
ExpressionAtlasi | Q9DDE3, baseline |
Family and domain databases
InterProi | View protein in InterPro IPR029058, AB_hydrolase IPR014788, AChE_tetra IPR002018, CarbesteraseB IPR019826, Carboxylesterase_B_AS IPR019819, Carboxylesterase_B_CS IPR000997, Cholinesterase |
Pfami | View protein in Pfam PF08674, AChE_tetra, 1 hit PF00135, COesterase, 1 hit |
PRINTSi | PR00878, CHOLNESTRASE |
SUPFAMi | SSF53474, SSF53474, 1 hit |
PROSITEi | View protein in PROSITE PS00122, CARBOXYLESTERASE_B_1, 1 hit PS00941, CARBOXYLESTERASE_B_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | ACES_DANRE | |
Accessioni | Q9DDE3Primary (citable) accession number: Q9DDE3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2001 |
Last sequence update: | March 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 135 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- SIMILARITY comments
Index of protein domains and families