UniProtKB - Q9DCW4 (ETFB_MOUSE)
Electron transfer flavoprotein subunit beta
Etfb
Functioni
Heterodimeric electron transfer flavoprotein that accepts electrons from several mitochondrial dehydrogenases, including acyl-CoA dehydrogenases, glutaryl-CoA and sarcosine dehydrogenase. It transfers the electrons to the main mitochondrial respiratory chain via ETF-ubiquinone oxidoreductase (By similarity).
Required for normal mitochondrial fatty acid oxidation and normal amino acid metabolism (PubMed:25023281).
ETFB binds an AMP molecule that probably has a purely structural role (By similarity).
By similarity1 PublicationSites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 9 | AMP; via carbonyl oxygenBy similarity | 1 | |
Binding sitei | 66 | AMP; via amide nitrogen and carbonyl oxygenBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 39 – 42 | AMPBy similarity | 4 | |
Nucleotide bindingi | 123 – 134 | AMPBy similarityAdd BLAST | 12 |
GO - Molecular functioni
- electron transfer activity Source: UniProtKB
- nucleotide binding Source: UniProtKB-KW
GO - Biological processi
- cellular amino acid catabolic process Source: MGI
- fatty acid beta-oxidation using acyl-CoA dehydrogenase Source: UniProtKB
- respiratory electron transport chain Source: MGI
Keywordsi
Biological process | Electron transport, Transport |
Ligand | Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-611105, Respiratory electron transport R-MMU-8876725, Protein methylation |
Names & Taxonomyi
Protein namesi | Recommended name: Electron transfer flavoprotein subunit betaCuratedShort name: Beta-ETFBy similarity |
Gene namesi | Name:EtfbImported |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:106098, Etfb |
VEuPathDBi | HostDB:ENSMUSG00000004610 |
Subcellular locationi
Mitochondrion
- Mitochondrion matrix By similarity
Mitochondrion
- mitochondrial electron transfer flavoprotein complex Source: MGI
- mitochondrial matrix Source: UniProtKB
- mitochondrion Source: MGI
Other locations
- electron transfer flavoprotein complex Source: MGI
Keywords - Cellular componenti
MitochondrionPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | Removed1 Publication | |||
ChainiPRO_0000167871 | 2 – 255 | Electron transfer flavoprotein subunit betaAdd BLAST | 254 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanine1 Publication | 1 | |
Modified residuei | 200 | N6,N6,N6-trimethyllysine; by ETFBKMT; alternate1 Publication | 1 | |
Modified residuei | 200 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 200 | N6-methyllysine; alternateBy similarity | 1 | |
Modified residuei | 203 | N6,N6,N6-trimethyllysine; by ETFBKMT1 Publication | 1 | |
Modified residuei | 210 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 210 | N6-succinyllysine; alternateCombined sources | 1 | |
Modified residuei | 223 | PhosphoserineBy similarity | 1 | |
Modified residuei | 226 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 248 | N6-acetyllysine; alternateCombined sources | 1 | |
Modified residuei | 248 | N6-succinyllysine; alternateCombined sources | 1 |
Post-translational modificationi
Keywords - PTMi
Acetylation, Methylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3801 |
EPDi | Q9DCW4 |
jPOSTi | Q9DCW4 |
MaxQBi | Q9DCW4 |
PaxDbi | Q9DCW4 |
PeptideAtlasi | Q9DCW4 |
PRIDEi | Q9DCW4 |
ProteomicsDBi | 275895 |
TopDownProteomicsi | Q9DCW4 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9DCW4 |
PTM databases
iPTMneti | Q9DCW4 |
PhosphoSitePlusi | Q9DCW4 |
SwissPalmi | Q9DCW4 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000004610, Expressed in adult mammalian kidney and 83 other tissues |
Genevisiblei | Q9DCW4, MM |
Interactioni
Subunit structurei
Heterodimer composed of ETFA and ETFB.
Identified in a complex that contains ETFA, ETFB and ETFRF1.
Interacts with ACADM.
By similarityProtein-protein interaction databases
BioGRIDi | 225938, 30 interactors |
ComplexPortali | CPX-865, Electron transfer flavoprotein complex |
IntActi | Q9DCW4, 5 interactors |
MINTi | Q9DCW4 |
STRINGi | 10090.ENSMUSP00000004729 |
Miscellaneous databases
RNActi | Q9DCW4, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 183 – 205 | Recognition loopBy similarityAdd BLAST | 23 |
Domaini
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG3180, Eukaryota |
GeneTreei | ENSGT00390000009936 |
HOGENOMi | CLU_060196_0_0_1 |
InParanoidi | Q9DCW4 |
OMAi | YNGGMVP |
OrthoDBi | 1092876at2759 |
PhylomeDBi | Q9DCW4 |
TreeFami | TF314039 |
Family and domain databases
CDDi | cd01714, ETF_beta, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR000049, ET-Flavoprotein_bsu_CS IPR014730, ETF_a/b_N IPR012255, ETF_b IPR033948, ETF_beta_N IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR21294, PTHR21294, 1 hit |
Pfami | View protein in Pfam PF01012, ETF, 1 hit |
PIRSFi | PIRSF000090, Beta-ETF, 1 hit |
SMARTi | View protein in SMART SM00893, ETF, 1 hit |
PROSITEi | View protein in PROSITE PS01065, ETF_BETA, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 4 potential isoforms that are computationally mapped.Show allAlign All
10 20 30 40 50
MAELRALVAV KRVIDFAVKI RVKPDKSGVV TDGVKHSMNP FCEIAVEEAV
60 70 80 90 100
RLKEKKLVKE IIAVSCGPSQ CQETIRTALA MGADRGIHVE IPGAQAESLG
110 120 130 140 150
PLQVARVLAK LAEKEKVDLL FLGKQAIDDD CNQTGQMTAG LLDWPQGTFA
160 170 180 190 200
SQVTLEGDKV KVEREIDGGL ETLRLKLPAV VTADLRLNEP RYATLPNIMK
210 220 230 240 250
AKKKKIEVVK AGDLGVDLTS KVSVISVEEP PQRSAGVKVE TTEDLVAKLK
EVGRI
Computationally mapped potential isoform sequencesi
There are 4 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A0U1RNP5 | A0A0U1RNP5_MOUSE | Electron transfer flavoprotein subu... | Etfb | 213 | Annotation score: | ||
A0A0U1RNK9 | A0A0U1RNK9_MOUSE | Electron transfer flavoprotein subu... | Etfb | 177 | Annotation score: | ||
A0A0U1RNR3 | A0A0U1RNR3_MOUSE | Electron transfer flavoprotein subu... | Etfb | 177 | Annotation score: | ||
A0A0U1RQB4 | A0A0U1RQB4_MOUSE | Electron transfer flavoprotein subu... | Etfb | 100 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 2 – 4 | Missing in BAB22076 (PubMed:16141072).Curated | 3 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002407 mRNA Translation: BAB22076.1 AK150293 mRNA Translation: BAE29447.1 BC069877 mRNA Translation: AAH69877.1 BC049237 mRNA Translation: AAH49237.1 |
CCDSi | CCDS21171.1 |
RefSeqi | NP_080971.2, NM_026695.3 |
Genome annotation databases
Ensembli | ENSMUST00000004729; ENSMUSP00000004729; ENSMUSG00000004610 |
GeneIDi | 110826 |
KEGGi | mmu:110826 |
UCSCi | uc009gmt.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002407 mRNA Translation: BAB22076.1 AK150293 mRNA Translation: BAE29447.1 BC069877 mRNA Translation: AAH69877.1 BC049237 mRNA Translation: AAH49237.1 |
CCDSi | CCDS21171.1 |
RefSeqi | NP_080971.2, NM_026695.3 |
3D structure databases
AlphaFoldDBi | Q9DCW4 |
SMRi | Q9DCW4 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 225938, 30 interactors |
ComplexPortali | CPX-865, Electron transfer flavoprotein complex |
IntActi | Q9DCW4, 5 interactors |
MINTi | Q9DCW4 |
STRINGi | 10090.ENSMUSP00000004729 |
PTM databases
iPTMneti | Q9DCW4 |
PhosphoSitePlusi | Q9DCW4 |
SwissPalmi | Q9DCW4 |
2D gel databases
REPRODUCTION-2DPAGEi | Q9DCW4 |
Proteomic databases
CPTACi | non-CPTAC-3801 |
EPDi | Q9DCW4 |
jPOSTi | Q9DCW4 |
MaxQBi | Q9DCW4 |
PaxDbi | Q9DCW4 |
PeptideAtlasi | Q9DCW4 |
PRIDEi | Q9DCW4 |
ProteomicsDBi | 275895 |
TopDownProteomicsi | Q9DCW4 |
Genome annotation databases
Ensembli | ENSMUST00000004729; ENSMUSP00000004729; ENSMUSG00000004610 |
GeneIDi | 110826 |
KEGGi | mmu:110826 |
UCSCi | uc009gmt.2, mouse |
Organism-specific databases
CTDi | 2109 |
MGIi | MGI:106098, Etfb |
VEuPathDBi | HostDB:ENSMUSG00000004610 |
Phylogenomic databases
eggNOGi | KOG3180, Eukaryota |
GeneTreei | ENSGT00390000009936 |
HOGENOMi | CLU_060196_0_0_1 |
InParanoidi | Q9DCW4 |
OMAi | YNGGMVP |
OrthoDBi | 1092876at2759 |
PhylomeDBi | Q9DCW4 |
TreeFami | TF314039 |
Enzyme and pathway databases
Reactomei | R-MMU-611105, Respiratory electron transport R-MMU-8876725, Protein methylation |
Miscellaneous databases
BioGRID-ORCSi | 110826, 2 hits in 72 CRISPR screens |
ChiTaRSi | Etfb, mouse |
PROi | PR:Q9DCW4 |
RNActi | Q9DCW4, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000004610, Expressed in adult mammalian kidney and 83 other tissues |
Genevisiblei | Q9DCW4, MM |
Family and domain databases
CDDi | cd01714, ETF_beta, 1 hit |
Gene3Di | 3.40.50.620, 1 hit |
InterProi | View protein in InterPro IPR000049, ET-Flavoprotein_bsu_CS IPR014730, ETF_a/b_N IPR012255, ETF_b IPR033948, ETF_beta_N IPR014729, Rossmann-like_a/b/a_fold |
PANTHERi | PTHR21294, PTHR21294, 1 hit |
Pfami | View protein in Pfam PF01012, ETF, 1 hit |
PIRSFi | PIRSF000090, Beta-ETF, 1 hit |
SMARTi | View protein in SMART SM00893, ETF, 1 hit |
PROSITEi | View protein in PROSITE PS01065, ETF_BETA, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | ETFB_MOUSE | |
Accessioni | Q9DCW4Primary (citable) accession number: Q9DCW4 Secondary accession number(s): Q810V3 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 11, 2002 |
Last sequence update: | January 23, 2007 | |
Last modified: | May 25, 2022 | |
This is version 159 of the entry and version 3 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Direct protein sequencing, Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families