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Protein

1,5-anhydro-D-fructose reductase

Gene

Akr1e2

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol.1 Publication

Catalytic activityi

1,5-anhydro-D-glucitol + NADP+ = 1,5-anhydro-D-fructose + NADPH.1 Publication

Enzyme regulationi

Inhibited by p-chloromercuribenzoic acid and alkyliodines.By similarity

Kineticsi

  1. KM=1.02 mM for 1,5-anhydro-D-fructose1 Publication
  1. Vmax=0.57 µmol/min/mg enzyme1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei35NADPBy similarity1
Active sitei40Proton donorBy similarity1
Sitei69Lowers pKa of active site TyrBy similarity1
Binding sitei102SubstrateBy similarity1
Binding sitei175NADPBy similarity1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi246 – 258NADPBy similarityAdd BLAST13

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionOxidoreductase
LigandNADP

Enzyme and pathway databases

BRENDAi1.1.1.292 3474
SABIO-RKiQ9DCT1

Names & Taxonomyi

Protein namesi
Recommended name:
1,5-anhydro-D-fructose reductase (EC:1.1.1.2631 Publication)
Short name:
AF reductase
Alternative name(s):
Aldo-keto reductase family 1 member C-like protein 2
Aldo-keto reductase family 1 member E1
Aldo-keto reductase family 1 member E2
Gene namesi
Name:Akr1e2
Synonyms:Akr1cl2, Akr1e1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 13

Organism-specific databases

MGIiMGI:1914758 Akr1e1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001246731 – 3011,5-anhydro-D-fructose reductaseAdd BLAST301

Proteomic databases

EPDiQ9DCT1
MaxQBiQ9DCT1
PaxDbiQ9DCT1
PeptideAtlasiQ9DCT1
PRIDEiQ9DCT1

PTM databases

iPTMnetiQ9DCT1
PhosphoSitePlusiQ9DCT1

Expressioni

Gene expression databases

BgeeiENSMUSG00000045410
CleanExiMM_AKR1E1
ExpressionAtlasiQ9DCT1 baseline and differential
GenevisibleiQ9DCT1 MM

Interactioni

Subunit structurei

Monomer.By similarity

Protein-protein interaction databases

IntActiQ9DCT1, 1 interactor
MINTiQ9DCT1
STRINGi10090.ENSMUSP00000089459

Structurei

3D structure databases

ProteinModelPortaliQ9DCT1
SMRiQ9DCT1
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the aldo/keto reductase family.Curated

Phylogenomic databases

eggNOGiKOG1577 Eukaryota
COG0656 LUCA
GeneTreeiENSGT00760000119041
HOGENOMiHOG000250272
InParanoidiQ9DCT1
KOiK13981
OMAiAYFYHNE
OrthoDBiEOG091G0D69
PhylomeDBiQ9DCT1
TreeFamiTF106492

Family and domain databases

CDDicd06660 Aldo_ket_red, 1 hit
Gene3Di3.20.20.100, 1 hit
InterProiView protein in InterPro
IPR018170 Aldo/ket_reductase_CS
IPR020471 Aldo/keto_reductase
IPR023210 NADP_OxRdtase_dom
IPR036812 NADP_OxRdtase_dom_sf
PANTHERiPTHR11732 PTHR11732, 1 hit
PfamiView protein in Pfam
PF00248 Aldo_ket_red, 1 hit
PIRSFiPIRSF000097 AKR, 1 hit
PRINTSiPR00069 ALDKETRDTASE
SUPFAMiSSF51430 SSF51430, 1 hit
PROSITEiView protein in PROSITE
PS00798 ALDOKETO_REDUCTASE_1, 1 hit
PS00062 ALDOKETO_REDUCTASE_2, 1 hit
PS00063 ALDOKETO_REDUCTASE_3, 1 hit

Sequencei

Sequence statusi: Complete.

Q9DCT1-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENIPTVGLG TWKASPGEVT DAVKLAINLG YRHFDCAYLY HNESEVGMGI
60 70 80 90 100
SEKIKEGVVK REDLFVVSKL WCTCHKKSLV KTACTNTLEA LNLDYLDLYL
110 120 130 140 150
IHWPMGFKPG EKDIPLDRNG KVIPSHTSFL DTWEAMEDLV FEGLVKNLGV
160 170 180 190 200
SNFNHEQLER LLDKPGLRVR PITNQIECHP YLNQKKLIDF CHKRNVSVTA
210 220 230 240 250
YRPLGGSGGG FHLMDDTVIR KIAKKHGKSP AQILIRFQIQ RNLIVIPKSV
260 270 280 290 300
TPSRIRENIQ VFDFELTEKD MEELLSLDKN LRLATFPTTE NHQDYPFHIE

Y
Length:301
Mass (Da):34,461
Last modified:June 1, 2001 - v1
Checksum:i5633663685EF32CA
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti105M → I in AAB37274 (Ref. 1) Curated1
Sequence conflicti283L → F in AAB37274 (Ref. 1) Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U68535 mRNA Translation: AAB37274.1
AK002507 mRNA Translation: BAB22152.1
BC012692 mRNA Translation: AAH12692.1
CCDSiCCDS26226.1
RefSeqiNP_061347.2, NM_018859.2
UniGeneiMm.251908

Genome annotation databases

EnsembliENSMUST00000091848; ENSMUSP00000089459; ENSMUSG00000045410
GeneIDi56043
KEGGimmu:56043
UCSCiuc007pjs.1 mouse

Similar proteinsi

Entry informationi

Entry nameiAKCL2_MOUSE
AccessioniPrimary (citable) accession number: Q9DCT1
Secondary accession number(s): O09125
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 10, 2002
Last sequence update: June 1, 2001
Last modified: February 28, 2018
This is version 124 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

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