UniProtKB - Q9DCT1 (AKCL2_MOUSE)
Protein
1,5-anhydro-D-fructose reductase
Gene
Akr1e2
Organism
Mus musculus (Mouse)
Status
Functioni
Catalyzes the NADPH-dependent reduction of 1,5-anhydro-D-fructose (AF) to 1,5-anhydro-D-glucitol.1 Publication
Catalytic activityi
- EC:1.1.1.2631 Publication
Activity regulationi
Inhibited by p-chloromercuribenzoic acid and alkyliodines.By similarity
Kineticsi
- KM=1.02 mM for 1,5-anhydro-D-fructose1 Publication
- Vmax=0.57 µmol/min/mg enzyme1 Publication
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 35 | NADPBy similarity | 1 | |
Active sitei | 40 | Proton donorBy similarity | 1 | |
Sitei | 69 | Lowers pKa of active site TyrBy similarity | 1 | |
Binding sitei | 102 | SubstrateBy similarity | 1 | |
Binding sitei | 175 | NADPBy similarity | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 246 – 258 | NADPBy similarityAdd BLAST | 13 |
GO - Molecular functioni
- 1,5-anhydro-D-fructose reductase activity Source: UniProtKB-EC
- alcohol dehydrogenase (NADP+) activity Source: GO_Central
- alditol:NADP+ 1-oxidoreductase activity Source: GO_Central
- oxidoreductase activity Source: MGI
GO - Biological processi
- oxidation-reduction process Source: MGI
Keywordsi
Molecular function | Oxidoreductase |
Ligand | NADP |
Enzyme and pathway databases
BRENDAi | 1.1.1.292, 3474 |
SABIO-RKi | Q9DCT1 |
Names & Taxonomyi
Protein namesi | Recommended name: 1,5-anhydro-D-fructose reductase (EC:1.1.1.2631 Publication)Short name: AF reductase Alternative name(s): Aldo-keto reductase family 1 member C-like protein 2 Aldo-keto reductase family 1 member E1 Aldo-keto reductase family 1 member E2 |
Gene namesi | Name:Akr1e2 Synonyms:Akr1cl2, Akr1e1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914758, Akr1e1 |
Subcellular locationi
Other locations
- Cytoplasm Curated
Cytosol
- cytosol Source: MGI
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
CytoplasmPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000124673 | 1 – 301 | 1,5-anhydro-D-fructose reductaseAdd BLAST | 301 |
Proteomic databases
EPDi | Q9DCT1 |
jPOSTi | Q9DCT1 |
MaxQBi | Q9DCT1 |
PaxDbi | Q9DCT1 |
PeptideAtlasi | Q9DCT1 |
PRIDEi | Q9DCT1 |
PTM databases
iPTMneti | Q9DCT1 |
PhosphoSitePlusi | Q9DCT1 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000045410, Expressed in undifferentiated genital tubercle and 276 other tissues |
Genevisiblei | Q9DCT1, MM |
Interactioni
Subunit structurei
Monomer.
By similarityProtein-protein interaction databases
IntActi | Q9DCT1, 1 interactor |
MINTi | Q9DCT1 |
STRINGi | 10090.ENSMUSP00000089459 |
Miscellaneous databases
RNActi | Q9DCT1, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the aldo/keto reductase family.Curated
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153272 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | Q9DCT1 |
OMAi | LTNQIEC |
OrthoDBi | 1016440at2759 |
PhylomeDBi | Q9DCT1 |
TreeFami | TF106492 |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 1 potential isoform that is computationally mapped.Show allAlign All
Q9DCT1-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MENIPTVGLG TWKASPGEVT DAVKLAINLG YRHFDCAYLY HNESEVGMGI
60 70 80 90 100
SEKIKEGVVK REDLFVVSKL WCTCHKKSLV KTACTNTLEA LNLDYLDLYL
110 120 130 140 150
IHWPMGFKPG EKDIPLDRNG KVIPSHTSFL DTWEAMEDLV FEGLVKNLGV
160 170 180 190 200
SNFNHEQLER LLDKPGLRVR PITNQIECHP YLNQKKLIDF CHKRNVSVTA
210 220 230 240 250
YRPLGGSGGG FHLMDDTVIR KIAKKHGKSP AQILIRFQIQ RNLIVIPKSV
260 270 280 290 300
TPSRIRENIQ VFDFELTEKD MEELLSLDKN LRLATFPTTE NHQDYPFHIE
Y
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketQ8CEB6 | Q8CEB6_MOUSE | Aldo-keto reductase family 1, membe... | Akr1e1 | 245 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 105 | M → I in AAB37274 (Ref. 1) Curated | 1 | |
Sequence conflicti | 283 | L → F in AAB37274 (Ref. 1) Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68535 mRNA Translation: AAB37274.1 AK002507 mRNA Translation: BAB22152.1 BC012692 mRNA Translation: AAH12692.1 |
CCDSi | CCDS26226.1 |
RefSeqi | NP_061347.2, NM_018859.2 |
Genome annotation databases
Ensembli | ENSMUST00000091848; ENSMUSP00000089459; ENSMUSG00000045410 |
GeneIDi | 56043 |
KEGGi | mmu:56043 |
UCSCi | uc007pjs.1, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | U68535 mRNA Translation: AAB37274.1 AK002507 mRNA Translation: BAB22152.1 BC012692 mRNA Translation: AAH12692.1 |
CCDSi | CCDS26226.1 |
RefSeqi | NP_061347.2, NM_018859.2 |
3D structure databases
SMRi | Q9DCT1 |
ModBasei | Search... |
Protein-protein interaction databases
IntActi | Q9DCT1, 1 interactor |
MINTi | Q9DCT1 |
STRINGi | 10090.ENSMUSP00000089459 |
PTM databases
iPTMneti | Q9DCT1 |
PhosphoSitePlusi | Q9DCT1 |
Proteomic databases
EPDi | Q9DCT1 |
jPOSTi | Q9DCT1 |
MaxQBi | Q9DCT1 |
PaxDbi | Q9DCT1 |
PeptideAtlasi | Q9DCT1 |
PRIDEi | Q9DCT1 |
Protocols and materials databases
Antibodypediai | 23956, 162 antibodies |
DNASUi | 56043 |
Genome annotation databases
Ensembli | ENSMUST00000091848; ENSMUSP00000089459; ENSMUSG00000045410 |
GeneIDi | 56043 |
KEGGi | mmu:56043 |
UCSCi | uc007pjs.1, mouse |
Organism-specific databases
CTDi | 56043 |
MGIi | MGI:1914758, Akr1e1 |
Phylogenomic databases
eggNOGi | KOG1577, Eukaryota |
GeneTreei | ENSGT00940000153272 |
HOGENOMi | CLU_023205_0_0_1 |
InParanoidi | Q9DCT1 |
OMAi | LTNQIEC |
OrthoDBi | 1016440at2759 |
PhylomeDBi | Q9DCT1 |
TreeFami | TF106492 |
Enzyme and pathway databases
BRENDAi | 1.1.1.292, 3474 |
SABIO-RKi | Q9DCT1 |
Miscellaneous databases
BioGRID-ORCSi | 56043, 0 hits in 17 CRISPR screens |
PROi | PR:Q9DCT1 |
RNActi | Q9DCT1, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000045410, Expressed in undifferentiated genital tubercle and 276 other tissues |
Genevisiblei | Q9DCT1, MM |
Family and domain databases
CDDi | cd06660, Aldo_ket_red, 1 hit |
Gene3Di | 3.20.20.100, 1 hit |
InterProi | View protein in InterPro IPR018170, Aldo/ket_reductase_CS IPR020471, Aldo/keto_reductase IPR023210, NADP_OxRdtase_dom IPR036812, NADP_OxRdtase_dom_sf |
PANTHERi | PTHR11732, PTHR11732, 1 hit |
Pfami | View protein in Pfam PF00248, Aldo_ket_red, 1 hit |
PIRSFi | PIRSF000097, AKR, 1 hit |
PRINTSi | PR00069, ALDKETRDTASE |
SUPFAMi | SSF51430, SSF51430, 1 hit |
PROSITEi | View protein in PROSITE PS00798, ALDOKETO_REDUCTASE_1, 1 hit PS00062, ALDOKETO_REDUCTASE_2, 1 hit PS00063, ALDOKETO_REDUCTASE_3, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AKCL2_MOUSE | |
Accessioni | Q9DCT1Primary (citable) accession number: Q9DCT1 Secondary accession number(s): O09125 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2002 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 137 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families