UniProtKB - Q9DCR2 (AP3S1_MOUSE)
Protein
AP-3 complex subunit sigma-1
Gene
Ap3s1
Organism
Mus musculus (Mouse)
Status
Functioni
Part of the AP-3 complex, an adaptor-related complex which is not clathrin-associated. The complex is associated with the Golgi region as well as more peripheral structures. It facilitates the budding of vesicles from the Golgi membrane and may be directly involved in trafficking to lysosomes. In concert with the BLOC-1 complex, AP-3 is required to target cargos into vesicles assembled at cell bodies for delivery into neurites and nerve terminals.1 Publication
GO - Biological processi
- anterograde axonal transport Source: UniProtKB
- anterograde synaptic vesicle transport Source: UniProtKB
- intracellular protein transport Source: MGI
- vesicle-mediated transport Source: GO_Central
Keywordsi
Biological process | Protein transport, Transport |
Enzyme and pathway databases
Reactomei | R-MMU-432722, Golgi Associated Vesicle Biogenesis |
Names & Taxonomyi
Protein namesi | Recommended name: AP-3 complex subunit sigma-1Alternative name(s): AP-3 complex subunit sigma-3A Adaptor-related protein complex 3 subunit sigma-1 Sigma-3A-adaptin Short name: Sigma3A-adaptin Sigma-adaptin 3a |
Gene namesi | Name:Ap3s1 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1337062, Ap3s1 |
Subcellular locationi
Golgi apparatus
Other locations
- Cytoplasmic vesicle membrane By similarity; Peripheral membrane protein By similarity; Cytoplasmic side By similarity
Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.By similarity
Golgi apparatus
- trans-Golgi network Source: MGI
Other locations
- AP-3 adaptor complex Source: MGI
- axon cytoplasm Source: GOC
- cytoplasmic vesicle membrane Source: UniProtKB-SubCell
- intracellular membrane-bounded organelle Source: MGI
- membrane Source: CACAO
Keywords - Cellular componenti
Cytoplasmic vesicle, Golgi apparatus, MembranePTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000193816 | 1 – 193 | AP-3 complex subunit sigma-1Add BLAST | 193 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 191 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9DCR2 |
MaxQBi | Q9DCR2 |
PaxDbi | Q9DCR2 |
PRIDEi | Q9DCR2 |
PTM databases
iPTMneti | Q9DCR2 |
PhosphoSitePlusi | Q9DCR2 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000024480, Expressed in pancreas and 59 other tissues |
ExpressionAtlasi | Q9DCR2, baseline and differential |
Genevisiblei | Q9DCR2, MM |
Interactioni
Subunit structurei
Adaptor protein complex 3 (AP-3) is a heterotetramer composed of two large adaptins (delta-type subunit AP3D1 and beta-type subunit AP3B1 or AP3B2), a medium adaptin (mu-type subunit AP3M1 or AP3M2) and a small adaptin (sigma-type subunit APS1 or AP3S2) (By similarity). AP-3 associates with the BLOC-1 complex.
Interacts with AGAP1.
By similarity1 PublicationProtein-protein interaction databases
BioGRIDi | 198135, 19 interactors |
ComplexPortali | CPX-5145, Ubiquitous AP-3 Adaptor complex, sigma3a variant CPX-5147, Neuronal AP-3 Adaptor complex, sigma3a variant |
IntActi | Q9DCR2, 4 interactors |
MINTi | Q9DCR2 |
STRINGi | 10090.ENSMUSP00000025357 |
Miscellaneous databases
RNActi | Q9DCR2, protein |
Family & Domainsi
Sequence similaritiesi
Belongs to the adaptor complexes small subunit family.Curated
Phylogenomic databases
eggNOGi | KOG0936, Eukaryota |
GeneTreei | ENSGT00970000193421 |
HOGENOMi | CLU_061221_2_2_1 |
InParanoidi | Q9DCR2 |
OrthoDBi | 1458249at2759 |
PhylomeDBi | Q9DCR2 |
TreeFami | TF300189 |
Family and domain databases
InterProi | View protein in InterPro IPR016635, AP_complex_ssu IPR022775, AP_mu_sigma_su IPR000804, Clathrin_sm-chain_CS IPR011012, Longin-like_dom_sf |
PANTHERi | PTHR11753, PTHR11753, 1 hit |
Pfami | View protein in Pfam PF01217, Clat_adaptor_s, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00989, CLAT_ADAPTOR_S, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9DCR2-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MIKAILIFNN HGKPRLSKFY QPYSEDTQQQ IIRETFHLVS KRDENVCNFL
60 70 80 90 100
EGGLLIGGSD NKLIYRHYAT LYFVFCVDSS ESELGILDLI QVFVETLDKC
110 120 130 140 150
FENVCELDLI FHVDKVHNIL AEMVMGGMVL ETNMNEIVTQ IDAQNKLEKS
160 170 180 190
EAGLAGAPAR AVSAVKNMNL PEIPRNINIG DISIKVPNLP SFK
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A286YDU3 | A0A286YDU3_MOUSE | AP complex subunit sigma | Ap3s1 | 162 | Annotation score: | ||
A0A286YD58 | A0A286YD58_MOUSE | AP-3 complex subunit sigma-1 | Ap3s1 | 54 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 30 | Q → P in BAB22191 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084575 mRNA Translation: AAC72819.1 AK002565 mRNA Translation: BAB22191.1 BC012656 mRNA Translation: AAH12656.1 |
CCDSi | CCDS29236.1 |
RefSeqi | NP_033811.1, NM_009681.5 |
Genome annotation databases
Ensembli | ENSMUST00000025357; ENSMUSP00000025357; ENSMUSG00000024480 |
GeneIDi | 11777 |
KEGGi | mmu:11777 |
UCSCi | uc008evv.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF084575 mRNA Translation: AAC72819.1 AK002565 mRNA Translation: BAB22191.1 BC012656 mRNA Translation: AAH12656.1 |
CCDSi | CCDS29236.1 |
RefSeqi | NP_033811.1, NM_009681.5 |
3D structure databases
SMRi | Q9DCR2 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 198135, 19 interactors |
ComplexPortali | CPX-5145, Ubiquitous AP-3 Adaptor complex, sigma3a variant CPX-5147, Neuronal AP-3 Adaptor complex, sigma3a variant |
IntActi | Q9DCR2, 4 interactors |
MINTi | Q9DCR2 |
STRINGi | 10090.ENSMUSP00000025357 |
PTM databases
iPTMneti | Q9DCR2 |
PhosphoSitePlusi | Q9DCR2 |
Proteomic databases
EPDi | Q9DCR2 |
MaxQBi | Q9DCR2 |
PaxDbi | Q9DCR2 |
PRIDEi | Q9DCR2 |
Protocols and materials databases
Antibodypediai | 25448, 129 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000025357; ENSMUSP00000025357; ENSMUSG00000024480 |
GeneIDi | 11777 |
KEGGi | mmu:11777 |
UCSCi | uc008evv.2, mouse |
Organism-specific databases
CTDi | 1176 |
MGIi | MGI:1337062, Ap3s1 |
Phylogenomic databases
eggNOGi | KOG0936, Eukaryota |
GeneTreei | ENSGT00970000193421 |
HOGENOMi | CLU_061221_2_2_1 |
InParanoidi | Q9DCR2 |
OrthoDBi | 1458249at2759 |
PhylomeDBi | Q9DCR2 |
TreeFami | TF300189 |
Enzyme and pathway databases
Reactomei | R-MMU-432722, Golgi Associated Vesicle Biogenesis |
Miscellaneous databases
BioGRID-ORCSi | 11777, 5 hits in 18 CRISPR screens |
ChiTaRSi | Ap3s1, mouse |
PROi | PR:Q9DCR2 |
RNActi | Q9DCR2, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000024480, Expressed in pancreas and 59 other tissues |
ExpressionAtlasi | Q9DCR2, baseline and differential |
Genevisiblei | Q9DCR2, MM |
Family and domain databases
InterProi | View protein in InterPro IPR016635, AP_complex_ssu IPR022775, AP_mu_sigma_su IPR000804, Clathrin_sm-chain_CS IPR011012, Longin-like_dom_sf |
PANTHERi | PTHR11753, PTHR11753, 1 hit |
Pfami | View protein in Pfam PF01217, Clat_adaptor_s, 1 hit |
SUPFAMi | SSF64356, SSF64356, 1 hit |
PROSITEi | View protein in PROSITE PS00989, CLAT_ADAPTOR_S, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | AP3S1_MOUSE | |
Accessioni | Q9DCR2Primary (citable) accession number: Q9DCR2 Secondary accession number(s): O88670 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | July 19, 2003 |
Last sequence update: | July 19, 2003 | |
Last modified: | February 10, 2021 | |
This is version 146 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - SIMILARITY comments
Index of protein domains and families