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Protein

Multifunctional protein ADE2

Gene

Paics

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Catalytic activityi

ATP + 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + L-aspartate = ADP + phosphate + (S)-2-(5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamido)succinate.
5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate = 5-amino-1-(5-phospho-D-ribosyl)imidazole + CO2.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
  2. Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).
Proteins known to be involved in this subpathway in this organism are:
  1. Multifunctional protein ADE2 (Paics)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei101For SAICAR synthetase activityBy similarity1
Active sitei107For SAICAR synthetase activityBy similarity1
Active sitei215For SAICAR synthetase activityBy similarity1
Active sitei303For AIR carboxylase activityBy similarity1
Active sitei332For AIR carboxylase activityBy similarity1
Sitei334Essential for AIR carboxylase activityBy similarity1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionDecarboxylase, Ligase, Lyase, Multifunctional enzyme
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-MMU-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00130
UPA00074; UER00131

Names & Taxonomyi

Protein namesi
Recommended name:
Multifunctional protein ADE2
Including the following 2 domains:
Phosphoribosylaminoimidazole-succinocarboxamide synthase (EC:6.3.2.6)
Alternative name(s):
SAICAR synthetase
Phosphoribosylaminoimidazole carboxylase (EC:4.1.1.21)
Alternative name(s):
AIR carboxylase
Short name:
AIRC
Gene namesi
Name:Paics
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 5

Organism-specific databases

MGIiMGI:1914304 Paics

Subcellular locationi

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedBy similarity
ChainiPRO_00000750312 – 425Multifunctional protein ADE2Add BLAST424

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineBy similarity1
Modified residuei22PhosphotyrosineCombined sources1
Modified residuei36N6-acetyllysineCombined sources1
Modified residuei107PhosphoserineBy similarity1
Modified residuei238PhosphothreonineBy similarity1
Modified residuei247N6-acetyllysineBy similarity1
Modified residuei274PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DCL9
MaxQBiQ9DCL9
PaxDbiQ9DCL9
PeptideAtlasiQ9DCL9
PRIDEiQ9DCL9

PTM databases

iPTMnetiQ9DCL9
PhosphoSitePlusiQ9DCL9
SwissPalmiQ9DCL9

Expressioni

Gene expression databases

BgeeiENSMUSG00000029247
ExpressionAtlasiQ9DCL9 baseline and differential
GenevisibleiQ9DCL9 MM

Interactioni

Subunit structurei

Homooctamer.By similarity

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211906, 6 interactors
IntActiQ9DCL9, 3 interactors
STRINGi10090.ENSMUSP00000031160

Structurei

3D structure databases

ProteinModelPortaliQ9DCL9
SMRiQ9DCL9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni2 – 260SAICAR synthetaseAdd BLAST259
Regioni261 – 425AIR carboxylaseAdd BLAST165
Regioni261 – 266LinkerBy similarity6
Regioni267 – 425AIR carboxylaseBy similarityAdd BLAST159

Sequence similaritiesi

In the N-terminal section; belongs to the SAICAR synthetase family.Curated
In the C-terminal section; belongs to the AIR carboxylase family. Class II subfamily.Curated

Phylogenomic databases

eggNOGiKOG2835 Eukaryota
COG0041 LUCA
COG0152 LUCA
GeneTreeiENSGT00390000010172
HOGENOMiHOG000082628
HOVERGENiHBG008335
InParanoidiQ9DCL9
KOiK01587
OMAiWSDEQII
OrthoDBiEOG091G08DN
TreeFamiTF106384

Family and domain databases

Gene3Di3.40.50.7700, 1 hit
HAMAPiMF_02045 PurE_classII, 1 hit
MF_00137 SAICAR_synth, 1 hit
InterProiView protein in InterPro
IPR033626 PurE_classII
IPR000031 PurE_dom
IPR035893 PurE_sf
IPR028923 SAICAR_synt/ADE2_N
IPR018236 SAICAR_synthetase_CS
PfamiView protein in Pfam
PF00731 AIRC, 1 hit
PF01259 SAICAR_synt, 1 hit
SMARTiView protein in SMART
SM01001 AIRC, 1 hit
SUPFAMiSSF52255 SSF52255, 1 hit
PROSITEiView protein in PROSITE
PS01057 SAICAR_SYNTHETASE_1, 1 hit
PS01058 SAICAR_SYNTHETASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCL9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATAVVVNIG KKLYEGKTKE VYELLDTPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVQEGYK FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQDCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSDSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVSVAG RSNGLGPVLS GNTAYPVISC
360 370 380 390 400
PPITPDWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASILNTW ISLKQADKKV RQCNL
Length:425
Mass (Da):47,006
Last modified:July 27, 2011 - v4
Checksum:iCD2A4323EC02B218
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti5V → Y in BAB22273 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002669 mRNA Translation: BAB22273.1
AK010462 mRNA Translation: BAB26957.1
AK012118 mRNA Translation: BAB28044.1
AK146722 mRNA Translation: BAE27385.1
AK159398 mRNA Translation: BAE35051.1
AK166264 mRNA Translation: BAE38669.1
CH466524 Genomic DNA Translation: EDL37918.1
BC018153 mRNA Translation: AAH18153.1
BC052691 mRNA Translation: AAH52691.1
CCDSiCCDS19367.1
RefSeqiNP_080215.1, NM_025939.2
XP_006535243.1, XM_006535180.2
XP_006535244.1, XM_006535181.3
XP_006535245.1, XM_006535182.3
XP_006535246.1, XM_006535183.3
UniGeneiMm.182931
Mm.431705

Genome annotation databases

EnsembliENSMUST00000031160; ENSMUSP00000031160; ENSMUSG00000029247
ENSMUST00000117536; ENSMUSP00000112879; ENSMUSG00000029247
ENSMUST00000120912; ENSMUSP00000113483; ENSMUSG00000029247
GeneIDi67054
KEGGimmu:67054
UCSCiuc008xvn.2 mouse

Similar proteinsi

Entry informationi

Entry nameiPUR6_MOUSE
AccessioniPrimary (citable) accession number: Q9DCL9
Secondary accession number(s): Q9CQ38
Entry historyiIntegrated into UniProtKB/Swiss-Prot: August 14, 2001
Last sequence update: July 27, 2011
Last modified: July 18, 2018
This is version 139 of the entry and version 4 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

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