UniProtKB - Q9DCL9 (PUR6_MOUSE)
Protein
Multifunctional protein ADE2
Gene
Paics
Organism
Mus musculus (Mouse)
Status
Functioni
Catalytic activityi
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + ATP + L-aspartate = (2S)-2-[5-amino-1-(5-phospho-β-D-ribosyl)imidazole-4-carboxamido]succinate + ADP + 2 H+ + phosphateEC:6.3.2.6
- 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate + H+ = 5-amino-1-(5-phospho-β-D-ribosyl)imidazole + CO2EC:4.1.1.21
: IMP biosynthesis via de novo pathway Pathwayi
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate.Proteins known to be involved in the 2 steps of the subpathway in this organism are:
- Phosphoribosylaminoimidazolesuccinocarboxamide synthase (Paics), Phosphoribosylaminoimidazolesuccinocarboxamide synthase (Paics), Multifunctional protein ADE2 (Paics)
- Adenylosuccinate lyase (Adsl), Adenylosuccinate lyase (Adsl), Adenylosuccinase (Adsl), Adenylosuccinate lyase (Adsl)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide from 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Pathwayi: IMP biosynthesis via de novo pathway
This protein is involved in step 1 of the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route).Proteins known to be involved in this subpathway in this organism are:
- Multifunctional protein ADE2 (Paics)
View all proteins of this organism that are known to be involved in the subpathway that synthesizes 5-amino-1-(5-phospho-D-ribosyl)imidazole-4-carboxylate from 5-amino-1-(5-phospho-D-ribosyl)imidazole (carboxylase route), the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Active sitei | 101 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 107 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 215 | For SAICAR synthetase activityBy similarity | 1 | |
Active sitei | 303 | For AIR carboxylase activityBy similarity | 1 | |
Active sitei | 332 | For AIR carboxylase activityBy similarity | 1 | |
Sitei | 334 | Essential for AIR carboxylase activityBy similarity | 1 |
GO - Molecular functioni
- 5-amino-4-imidazole carboxylate lyase activity Source: UniProtKB-EC
- ATP binding Source: UniProtKB-KW
- identical protein binding Source: MGI
- phosphoribosylaminoimidazole carboxylase activity Source: UniProtKB-EC
- phosphoribosylaminoimidazolesuccinocarboxamide synthase activity Source: UniProtKB-EC
GO - Biological processi
- 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
Keywordsi
Molecular function | Decarboxylase, Ligase, Lyase, Multifunctional enzyme |
Biological process | Purine biosynthesis |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
Reactomei | R-MMU-73817, Purine ribonucleoside monophosphate biosynthesis |
UniPathwayi | UPA00074;UER00130 UPA00074;UER00131 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Paics |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914304, Paics |
Subcellular locationi
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Initiator methioninei | RemovedBy similarity | |||
ChainiPRO_0000075031 | 2 – 425 | Multifunctional protein ADE2Add BLAST | 424 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 2 | N-acetylalanineBy similarity | 1 | |
Modified residuei | 22 | PhosphotyrosineCombined sources | 1 | |
Modified residuei | 36 | N6-acetyllysineCombined sources | 1 | |
Modified residuei | 107 | PhosphoserineBy similarity | 1 | |
Modified residuei | 238 | PhosphothreonineBy similarity | 1 | |
Modified residuei | 247 | N6-acetyllysineBy similarity | 1 | |
Modified residuei | 274 | PhosphoserineBy similarity | 1 |
Keywords - PTMi
Acetylation, PhosphoproteinProteomic databases
CPTACi | non-CPTAC-3741 |
EPDi | Q9DCL9 |
jPOSTi | Q9DCL9 |
MaxQBi | Q9DCL9 |
PaxDbi | Q9DCL9 |
PeptideAtlasi | Q9DCL9 |
PRIDEi | Q9DCL9 |
PTM databases
iPTMneti | Q9DCL9 |
PhosphoSitePlusi | Q9DCL9 |
SwissPalmi | Q9DCL9 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000029247, Expressed in liver and 320 other tissues |
Genevisiblei | Q9DCL9, MM |
Interactioni
Subunit structurei
Homooctamer.
By similarityGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 211906, 23 interactors |
IntActi | Q9DCL9, 3 interactors |
STRINGi | 10090.ENSMUSP00000031160 |
Miscellaneous databases
RNActi | Q9DCL9, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 2 – 260 | SAICAR synthetaseAdd BLAST | 259 | |
Regioni | 261 – 425 | AIR carboxylaseAdd BLAST | 165 | |
Regioni | 261 – 266 | LinkerBy similarity | 6 | |
Regioni | 267 – 425 | AIR carboxylaseBy similarityAdd BLAST | 159 |
Sequence similaritiesi
In the N-terminal section; belongs to the SAICAR synthetase family.Curated
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
GeneTreei | ENSGT00390000010172 |
HOGENOMi | CLU_061495_1_0_1 |
InParanoidi | Q9DCL9 |
OMAi | WSDEQII |
OrthoDBi | 566769at2759 |
TreeFami | TF106384 |
Family and domain databases
Gene3Di | 3.40.50.7700, 1 hit |
HAMAPi | MF_02045, PurE_classII, 1 hit MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR033626, PurE_classII IPR000031, PurE_dom IPR035893, PurE_sf IPR028923, SAICAR_synt/ADE2_N IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF01259, SAICAR_synt, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF52255, SSF52255, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
(1+)i Sequence
Sequence statusi: Complete.
: The displayed sequence is further processed into a mature form. Sequence processingi
This entry has 1 described isoform and 2 potential isoforms that are computationally mapped.Show allAlign All
Q9DCL9-1 [UniParc]FASTAAdd to basket
10 20 30 40 50
MATAVVVNIG KKLYEGKTKE VYELLDTPGR VLLQSKDQIT AGNAARKNHL
60 70 80 90 100
EGKAAISNKI TSCIFQLLQE AGIKTAFTKK CGETAFIAPQ CEMIPIEWVC
110 120 130 140 150
RRIATGSFLK RNPGVQEGYK FYPPKVEMFF KDDANNDPQW SEEQLIAAKF
160 170 180 190 200
CFAGLVIGQT EVDIMSHATQ AIFEILEKSW LPQDCTLVDM KIEFGVDVTT
210 220 230 240 250
KEIVLADVID NDSWRLWPSG DRSQQKDKQS YRDLKEVTPE GLQMVKKNFE
260 270 280 290 300
WVADRVELLL KSDSQCRVVV LMGSTSDLGH CEKIKKACGN FGIPCELRVT
310 320 330 340 350
SAHKGPDETL RIKAEYEGDG IPTVFVSVAG RSNGLGPVLS GNTAYPVISC
360 370 380 390 400
PPITPDWGAQ DVWSSLRLPS GIGCSTILSP EGSAQFAAQI FGLNNHLVWA
410 420
KLRASILNTW ISLKQADKKV RQCNL
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketD3Z6P1 | D3Z6P1_MOUSE | Phosphoribosylaminoimidazolesuccino... | Paics | 83 | Annotation score: | ||
D6RCU8 | D6RCU8_MOUSE | Phosphoribosylaminoimidazolesuccino... | Paics | 103 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 5 | V → Y in BAB22273 (PubMed:16141072).Curated | 1 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002669 mRNA Translation: BAB22273.1 AK010462 mRNA Translation: BAB26957.1 AK012118 mRNA Translation: BAB28044.1 AK146722 mRNA Translation: BAE27385.1 AK159398 mRNA Translation: BAE35051.1 AK166264 mRNA Translation: BAE38669.1 CH466524 Genomic DNA Translation: EDL37918.1 BC018153 mRNA Translation: AAH18153.1 BC052691 mRNA Translation: AAH52691.1 |
CCDSi | CCDS19367.1 |
RefSeqi | NP_080215.1, NM_025939.2 XP_006535243.1, XM_006535180.2 XP_006535244.1, XM_006535181.3 XP_006535245.1, XM_006535182.3 XP_006535246.1, XM_006535183.3 |
Genome annotation databases
Ensembli | ENSMUST00000031160; ENSMUSP00000031160; ENSMUSG00000029247 ENSMUST00000117536; ENSMUSP00000112879; ENSMUSG00000029247 ENSMUST00000120912; ENSMUSP00000113483; ENSMUSG00000029247 |
GeneIDi | 67054 |
KEGGi | mmu:67054 |
UCSCi | uc008xvn.2, mouse |
Similar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK002669 mRNA Translation: BAB22273.1 AK010462 mRNA Translation: BAB26957.1 AK012118 mRNA Translation: BAB28044.1 AK146722 mRNA Translation: BAE27385.1 AK159398 mRNA Translation: BAE35051.1 AK166264 mRNA Translation: BAE38669.1 CH466524 Genomic DNA Translation: EDL37918.1 BC018153 mRNA Translation: AAH18153.1 BC052691 mRNA Translation: AAH52691.1 |
CCDSi | CCDS19367.1 |
RefSeqi | NP_080215.1, NM_025939.2 XP_006535243.1, XM_006535180.2 XP_006535244.1, XM_006535181.3 XP_006535245.1, XM_006535182.3 XP_006535246.1, XM_006535183.3 |
3D structure databases
SMRi | Q9DCL9 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211906, 23 interactors |
IntActi | Q9DCL9, 3 interactors |
STRINGi | 10090.ENSMUSP00000031160 |
PTM databases
iPTMneti | Q9DCL9 |
PhosphoSitePlusi | Q9DCL9 |
SwissPalmi | Q9DCL9 |
Proteomic databases
CPTACi | non-CPTAC-3741 |
EPDi | Q9DCL9 |
jPOSTi | Q9DCL9 |
MaxQBi | Q9DCL9 |
PaxDbi | Q9DCL9 |
PeptideAtlasi | Q9DCL9 |
PRIDEi | Q9DCL9 |
Protocols and materials databases
Antibodypediai | 24027, 314 antibodies |
DNASUi | 67054 |
Genome annotation databases
Ensembli | ENSMUST00000031160; ENSMUSP00000031160; ENSMUSG00000029247 ENSMUST00000117536; ENSMUSP00000112879; ENSMUSG00000029247 ENSMUST00000120912; ENSMUSP00000113483; ENSMUSG00000029247 |
GeneIDi | 67054 |
KEGGi | mmu:67054 |
UCSCi | uc008xvn.2, mouse |
Organism-specific databases
CTDi | 10606 |
MGIi | MGI:1914304, Paics |
Phylogenomic databases
eggNOGi | KOG2835, Eukaryota |
GeneTreei | ENSGT00390000010172 |
HOGENOMi | CLU_061495_1_0_1 |
InParanoidi | Q9DCL9 |
OMAi | WSDEQII |
OrthoDBi | 566769at2759 |
TreeFami | TF106384 |
Enzyme and pathway databases
UniPathwayi | UPA00074;UER00130 UPA00074;UER00131 |
Reactomei | R-MMU-73817, Purine ribonucleoside monophosphate biosynthesis |
Miscellaneous databases
BioGRID-ORCSi | 67054, 6 hits in 19 CRISPR screens |
ChiTaRSi | Paics, mouse |
PROi | PR:Q9DCL9 |
RNActi | Q9DCL9, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000029247, Expressed in liver and 320 other tissues |
Genevisiblei | Q9DCL9, MM |
Family and domain databases
Gene3Di | 3.40.50.7700, 1 hit |
HAMAPi | MF_02045, PurE_classII, 1 hit MF_00137, SAICAR_synth, 1 hit |
InterProi | View protein in InterPro IPR033626, PurE_classII IPR000031, PurE_dom IPR035893, PurE_sf IPR028923, SAICAR_synt/ADE2_N IPR018236, SAICAR_synthetase_CS |
Pfami | View protein in Pfam PF00731, AIRC, 1 hit PF01259, SAICAR_synt, 1 hit |
SMARTi | View protein in SMART SM01001, AIRC, 1 hit |
SUPFAMi | SSF52255, SSF52255, 1 hit |
PROSITEi | View protein in PROSITE PS01057, SAICAR_SYNTHETASE_1, 1 hit PS01058, SAICAR_SYNTHETASE_2, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | PUR6_MOUSE | |
Accessioni | Q9DCL9Primary (citable) accession number: Q9DCL9 Secondary accession number(s): Q9CQ38 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | August 14, 2001 |
Last sequence update: | July 27, 2011 | |
Last modified: | December 2, 2020 | |
This is version 155 of the entry and version 4 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PATHWAY comments
Index of metabolic and biosynthesis pathways - SIMILARITY comments
Index of protein domains and families