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Protein

Eukaryotic translation initiation factor 3 subunit F

Gene

Eif3f

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation
Deubiquitinates activated NOTCH1, promoting its nuclear import, thereby acting as a positive regulator of Notch signaling.UniRule annotation1 Publication

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionHydrolase, Initiation factor, Protease, Thiol protease
Biological processProtein biosynthesis, Ubl conjugation pathway

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Protein family/group databases

MEROPSiM67.974

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit FUniRule annotation
Short name:
eIF3fUniRule annotation
Alternative name(s):
Deubiquitinating enzyme eIF3f (EC:3.4.19.12)
Eukaryotic translation initiation factor 3 subunit 5UniRule annotation
eIF-3-epsilonUniRule annotation
eIF3 p47UniRule annotation
Gene namesi
Name:Eif3f
Synonyms:Eif3s5
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1913335 Eif3f

Subcellular locationi

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00002139652 – 361Eukaryotic translation initiation factor 3 subunit FAdd BLAST360

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationBy similarity1
Modified residuei52Phosphoserine; by CDK11; in vitroUniRule annotationBy similarity1
Modified residuei242N6-acetyllysineBy similarity1
Modified residuei262PhosphoserineUniRule annotationBy similarity1

Post-translational modificationi

Phosphorylation is enhanced upon serum stimulation. Phosphorylated during apoptosis by caspase-processed CDK11 (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DCH4
MaxQBiQ9DCH4
PaxDbiQ9DCH4
PRIDEiQ9DCH4

PTM databases

iPTMnetiQ9DCH4
PhosphoSitePlusiQ9DCH4
SwissPalmiQ9DCH4

Expressioni

Gene expression databases

BgeeiENSMUSG00000031029 Expressed in 283 organ(s), highest expression level in urinary bladder urothelium
GenevisibleiQ9DCH4 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex may interact with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation may lead to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Interacts with RNF139; the interaction leads to protein translation inhibitions in a ubiquitination-dependent manner. Interacts with DTX1, the interaction is required for deubiquitinating activity towards NOTCH1 (By similarity).UniRule annotation

Binary interactionsi

GO - Molecular functioni

Protein-protein interaction databases

BioGridi211203, 6 interactors
DIPiDIP-42766N
IntActiQ9DCH4, 12 interactors
MINTiQ9DCH4
STRINGi10090.ENSMUSP00000033342

Structurei

3D structure databases

ProteinModelPortaliQ9DCH4
SMRiQ9DCH4
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini96 – 226MPNPROSITE-ProRule annotationAdd BLAST131

Domaini

The MPN domain mediates deubiquitinating activity.By similarity

Sequence similaritiesi

Belongs to the eIF-3 subunit F family.UniRule annotation

Phylogenomic databases

eggNOGiKOG2975 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00530000063075
HOGENOMiHOG000241154
HOVERGENiHBG107843
InParanoidiQ9DCH4
KOiK03249
OMAiAVDMEYH
OrthoDBiEOG091G0EMR
TreeFamiTF101517

Family and domain databases

CDDicd08064 MPN_eIF3f, 1 hit
HAMAPiMF_03005 eIF3f, 1 hit
InterProiView protein in InterPro
IPR027531 eIF3f
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10540:SF6 PTHR10540:SF6, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

Q9DCH4-1 [UniParc]FASTAAdd to basket
« Hide
        10         20         30         40         50
MASPAVPANV PPATAAAAPA PVVTAAPASA PTPSTPAPTP AATPAASPAP
60 70 80 90 100
VSSDPAVAAP AAPGQTPASA PAPAQTPAPS QPGPALPGPF PGGRVVRLHP
110 120 130 140 150
VILASIVDSY ERRNEGAARV IGTLLGTVDK HSVEVTNCFS VPHNESEDEV
160 170 180 190 200
AVDMEFAKNM YELHKKVSPN ELILGWYATG HDITEHSVLI HEYYSREAPN
210 220 230 240 250
PIHLTVDTGL QHGRMSIKAY VSTLMGVPGR TMGVMFTPLT VKYAYYDTER
260 270 280 290 300
IGVDLIMKTC FSPNRVIGLS SDLQQVGGAS ARIQDALSTV LQYAEDVLSG
310 320 330 340 350
KVSADNTVGR FLMSLVNQVP KIVPDDFETM LNSNINDLLM VTYLANLTQS
360
QIALNEKLVN L
Length:361
Mass (Da):37,984
Last modified:July 27, 2011 - v2
Checksum:iD0BE3309F9141E40
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti198A → S in BAB22352 (PubMed:16141072).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002778 mRNA Translation: BAB22352.1
CH466531 Genomic DNA Translation: EDL16920.1
BC083190 mRNA Translation: AAH83190.1
CCDSiCCDS21731.1
RefSeqiNP_079620.2, NM_025344.2
UniGeneiMm.182962

Genome annotation databases

EnsembliENSMUST00000033342; ENSMUSP00000033342; ENSMUSG00000031029
GeneIDi66085
KEGGimmu:66085
UCSCiuc009jdc.2 mouse

Similar proteinsi

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK002778 mRNA Translation: BAB22352.1
CH466531 Genomic DNA Translation: EDL16920.1
BC083190 mRNA Translation: AAH83190.1
CCDSiCCDS21731.1
RefSeqiNP_079620.2, NM_025344.2
UniGeneiMm.182962

3D structure databases

ProteinModelPortaliQ9DCH4
SMRiQ9DCH4
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi211203, 6 interactors
DIPiDIP-42766N
IntActiQ9DCH4, 12 interactors
MINTiQ9DCH4
STRINGi10090.ENSMUSP00000033342

Protein family/group databases

MEROPSiM67.974

PTM databases

iPTMnetiQ9DCH4
PhosphoSitePlusiQ9DCH4
SwissPalmiQ9DCH4

Proteomic databases

EPDiQ9DCH4
MaxQBiQ9DCH4
PaxDbiQ9DCH4
PRIDEiQ9DCH4

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000033342; ENSMUSP00000033342; ENSMUSG00000031029
GeneIDi66085
KEGGimmu:66085
UCSCiuc009jdc.2 mouse

Organism-specific databases

CTDi8665
MGIiMGI:1913335 Eif3f

Phylogenomic databases

eggNOGiKOG2975 Eukaryota
COG1310 LUCA
GeneTreeiENSGT00530000063075
HOGENOMiHOG000241154
HOVERGENiHBG107843
InParanoidiQ9DCH4
KOiK03249
OMAiAVDMEYH
OrthoDBiEOG091G0EMR
TreeFamiTF101517

Enzyme and pathway databases

ReactomeiR-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Miscellaneous databases

ChiTaRSiEif3f mouse
PROiPR:Q9DCH4
SOURCEiSearch...

Gene expression databases

BgeeiENSMUSG00000031029 Expressed in 283 organ(s), highest expression level in urinary bladder urothelium
GenevisibleiQ9DCH4 MM

Family and domain databases

CDDicd08064 MPN_eIF3f, 1 hit
HAMAPiMF_03005 eIF3f, 1 hit
InterProiView protein in InterPro
IPR027531 eIF3f
IPR000555 JAMM/MPN+_dom
IPR037518 MPN
IPR024969 Rpn11/EIF3F_C
PANTHERiPTHR10540:SF6 PTHR10540:SF6, 1 hit
PfamiView protein in Pfam
PF01398 JAB, 1 hit
PF13012 MitMem_reg, 1 hit
SMARTiView protein in SMART
SM00232 JAB_MPN, 1 hit
PROSITEiView protein in PROSITE
PS50249 MPN, 1 hit
ProtoNetiSearch...

Entry informationi

Entry nameiEIF3F_MOUSE
AccessioniPrimary (citable) accession number: Q9DCH4
Secondary accession number(s): Q5XJV3
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: July 27, 2011
Last modified: October 10, 2018
This is version 125 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families
  2. Translation initiation factors
    List of translation initiation factor entries
  3. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
UniProt is an ELIXIR core data resource
Main funding by: National Institutes of Health

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