UniProtKB - Q9DC63 (FBX3_MOUSE)
Protein
F-box only protein 3
Gene
Fbxo3
Organism
Mus musculus (Mouse)
Status
Functioni
Substrate recognition component of the SCF (SKP1-CUL1-F-box protein)-type E3 ubiquitin ligase complex. Mediates the ubiquitination of HIPK2 and probably that of EP300, leading to rapid degradation by the proteasome. In the presence of PML, HIPK2 ubiquitination still occurs, but degradation is prevented. PML, HIPK2 and FBXO3 may act synergically to activate p53/TP53-dependent transactivation (By similarity).By similarity
Keywordsi
Biological process | Ubl conjugation pathway |
Names & Taxonomyi
Protein namesi | Recommended name: F-box only protein 3 |
Gene namesi | Name:Fbxo3 Synonyms:Fba, Fbx3 |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1929084, Fbxo3 |
Subcellular locationi
Nucleus
- Nucleus By similarity
Note: Colocalizes with PML at the peripheries of nuclear bodies.By similarity
Cytosol
- cytosol Source: MGI
Nucleus
- nucleoplasm Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000119878 | 1 – 480 | F-box only protein 3Add BLAST | 480 |
Proteomic databases
EPDi | Q9DC63 |
PaxDbi | Q9DC63 |
PeptideAtlasi | Q9DC63 |
PRIDEi | Q9DC63 |
PTM databases
iPTMneti | Q9DC63 |
PhosphoSitePlusi | Q9DC63 |
Expressioni
Gene expression databases
Bgeei | ENSMUSG00000027180, Expressed in vastus lateralis and 305 other tissues |
ExpressionAtlasi | Q9DC63, baseline and differential |
Genevisiblei | Q9DC63, MM |
Interactioni
Subunit structurei
Part of a SCF (SKP1-cullin-F-box) protein ligase complex consisting of FBXO3, SKP1, CUL1 and RBX1.
Interacts with PML, interaction is direct and takes place either alone or within the SCF complex (By similarity).
By similarityProtein-protein interaction databases
BioGRIDi | 208300, 17 interactors |
STRINGi | 10090.ENSMUSP00000028603 |
Miscellaneous databases
RNActi | Q9DC63, protein |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 10 – 56 | F-boxPROSITE-ProRule annotationAdd BLAST | 47 | |
Domaini | 278 – 408 | ApaGPROSITE-ProRule annotationAdd BLAST | 131 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 414 – 460 | Asp/Glu-rich (highly acidic)Add BLAST | 47 | |
Compositional biasi | 462 – 465 | Poly-Arg | 4 | |
Compositional biasi | 472 – 475 | Poly-Arg | 4 |
Phylogenomic databases
eggNOGi | KOG4408, Eukaryota |
GeneTreei | ENSGT00940000153571 |
InParanoidi | Q9DC63 |
OMAi | YRDLVSC |
PhylomeDBi | Q9DC63 |
TreeFami | TF329795 |
Family and domain databases
Gene3Di | 2.60.40.1470, 1 hit 3.40.1580.10, 1 hit |
InterProi | View protein in InterPro IPR007474, ApaG_domain IPR036767, ApaG_sf IPR036047, F-box-like_dom_sf IPR001810, F-box_dom IPR018958, Knr4/Smi1-like_dom IPR037883, Knr4/Smi1-like_sf |
Pfami | View protein in Pfam PF04379, DUF525, 1 hit PF12937, F-box-like, 1 hit PF09346, SMI1_KNR4, 1 hit |
SMARTi | View protein in SMART SM00256, FBOX, 1 hit SM00860, SMI1_KNR4, 1 hit |
SUPFAMi | SSF110069, SSF110069, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS51087, APAG, 1 hit PS50181, FBOX, 1 hit |
s (3+)i Sequence
Sequence statusi: Complete.
This entry describes 3 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 3 described isoforms and 1 potential isoform that is computationally mapped.Show allAlign All
Isoform 1 (identifier: Q9DC63-1) [UniParc]FASTAAdd to basket
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MAAVEAETGL LTLESLPTDP LLLILSFVDY RDLINCCYVS RRLSQLSTHD
60 70 80 90 100
PLWRRHCKKY WLISEEEKAG KSQCWRSLFI ETYSDVGRYI DHYAAIKKAW
110 120 130 140 150
RDLKKYLEPR CPRMVLSLKE GAREEDLDAV EAQIGCKLPD DYRCSYRIHN
160 170 180 190 200
GQKLVVPGLL GSMALSNHYR SEDLLDVDTA AGGFQQRQGL KYCLPLTFCI
210 220 230 240 250
HTGLSQYIAV EAAEGRNKNE VFYQCPDQMA RNPAAIDMFI IGATFTDWFT
260 270 280 290 300
SYVNNVVSGG FPIIRDQIFR YIHDPECVAT TGDITVSVST SFLPELSSVH
310 320 330 340 350
PPHYFFTYRI RIEMSRDALP EKACQLDSRY WRITNAKGDV EEVQGPGVVG
360 370 380 390 400
EFPIISPGRI YEYTSCTTFS TTSGYMEGYY TFHFLYFKDK VFNVAIPRFH
410 420 430 440 450
MACPTFRVSI ARLEMGPDEY EEMEEEAEEE EEEENDDSAD MDESDESDAD
460 470 480
ENESDEGEGE ARRRRVFDVP IRRRRCSRLF
Computationally mapped potential isoform sequencesi
There is 1 potential isoform mapped to this entry.BLASTAlignShow allAdd to basketA2BHN6 | A2BHN6_MOUSE | F-box only protein 3 | Fbxo3 | 361 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 63 | I → M in BAB31793 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 86 | V → M in BAC34118 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 214 | E → V in BAC25719 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 228 | Q → K in BAB31793 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 262 | P → H in BAE30746 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 262 | P → H in BAE30138 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 458 | E → A in BAE30746 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 458 | E → A in BAE30138 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_039615 | 36 – 40 | Missing in isoform 3. Curated | 5 | |
Alternative sequenceiVSP_039616 | 414 – 415 | EM → VS in isoform 2 and isoform 3. 1 Publication | 2 | |
Alternative sequenceiVSP_039617 | 416 – 480 | Missing in isoform 2 and isoform 3. 1 PublicationAdd BLAST | 65 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233226 mRNA Translation: AAF67156.1 AK004544 mRNA Translation: BAB23360.1 AK019550 mRNA Translation: BAB31793.1 AK028048 mRNA Translation: BAC25719.1 AK031428 mRNA Translation: BAC27398.1 AK049870 mRNA Translation: BAC33965.1 AK050195 mRNA Translation: BAC34118.1 AK151130 mRNA Translation: BAE30138.1 AK151857 mRNA Translation: BAE30746.1 AK152780 mRNA Translation: BAE31491.1 AK167075 mRNA Translation: BAE39235.1 BX640578 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27711.1 CH466519 Genomic DNA Translation: EDL27712.1 BC010212 mRNA Translation: AAH10212.2 BC058253 mRNA Translation: AAH58253.1 |
CCDSi | CCDS16484.1 [Q9DC63-1] CCDS16485.1 [Q9DC63-2] |
RefSeqi | NP_065618.1, NM_020593.2 [Q9DC63-2] NP_997598.1, NM_212433.1 [Q9DC63-1] |
Genome annotation databases
Ensembli | ENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180 [Q9DC63-1] ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180 [Q9DC63-2] ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180 [Q9DC63-3] |
GeneIDi | 57443 |
KEGGi | mmu:57443 |
UCSCi | uc008lji.1, mouse [Q9DC63-2] uc008ljk.1, mouse [Q9DC63-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AF233226 mRNA Translation: AAF67156.1 AK004544 mRNA Translation: BAB23360.1 AK019550 mRNA Translation: BAB31793.1 AK028048 mRNA Translation: BAC25719.1 AK031428 mRNA Translation: BAC27398.1 AK049870 mRNA Translation: BAC33965.1 AK050195 mRNA Translation: BAC34118.1 AK151130 mRNA Translation: BAE30138.1 AK151857 mRNA Translation: BAE30746.1 AK152780 mRNA Translation: BAE31491.1 AK167075 mRNA Translation: BAE39235.1 BX640578 Genomic DNA No translation available. CH466519 Genomic DNA Translation: EDL27711.1 CH466519 Genomic DNA Translation: EDL27712.1 BC010212 mRNA Translation: AAH10212.2 BC058253 mRNA Translation: AAH58253.1 |
CCDSi | CCDS16484.1 [Q9DC63-1] CCDS16485.1 [Q9DC63-2] |
RefSeqi | NP_065618.1, NM_020593.2 [Q9DC63-2] NP_997598.1, NM_212433.1 [Q9DC63-1] |
3D structure databases
SMRi | Q9DC63 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 208300, 17 interactors |
STRINGi | 10090.ENSMUSP00000028603 |
PTM databases
iPTMneti | Q9DC63 |
PhosphoSitePlusi | Q9DC63 |
Proteomic databases
EPDi | Q9DC63 |
PaxDbi | Q9DC63 |
PeptideAtlasi | Q9DC63 |
PRIDEi | Q9DC63 |
Protocols and materials databases
Antibodypediai | 25751, 129 antibodies |
Genome annotation databases
Ensembli | ENSMUST00000028603; ENSMUSP00000028603; ENSMUSG00000027180 [Q9DC63-1] ENSMUST00000102565; ENSMUSP00000099625; ENSMUSG00000027180 [Q9DC63-2] ENSMUST00000111135; ENSMUSP00000106765; ENSMUSG00000027180 [Q9DC63-3] |
GeneIDi | 57443 |
KEGGi | mmu:57443 |
UCSCi | uc008lji.1, mouse [Q9DC63-2] uc008ljk.1, mouse [Q9DC63-1] |
Organism-specific databases
CTDi | 26273 |
MGIi | MGI:1929084, Fbxo3 |
Phylogenomic databases
eggNOGi | KOG4408, Eukaryota |
GeneTreei | ENSGT00940000153571 |
InParanoidi | Q9DC63 |
OMAi | YRDLVSC |
PhylomeDBi | Q9DC63 |
TreeFami | TF329795 |
Miscellaneous databases
BioGRID-ORCSi | 57443, 3 hits in 17 CRISPR screens |
ChiTaRSi | Fbxo3, mouse |
PROi | PR:Q9DC63 |
RNActi | Q9DC63, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000027180, Expressed in vastus lateralis and 305 other tissues |
ExpressionAtlasi | Q9DC63, baseline and differential |
Genevisiblei | Q9DC63, MM |
Family and domain databases
Gene3Di | 2.60.40.1470, 1 hit 3.40.1580.10, 1 hit |
InterProi | View protein in InterPro IPR007474, ApaG_domain IPR036767, ApaG_sf IPR036047, F-box-like_dom_sf IPR001810, F-box_dom IPR018958, Knr4/Smi1-like_dom IPR037883, Knr4/Smi1-like_sf |
Pfami | View protein in Pfam PF04379, DUF525, 1 hit PF12937, F-box-like, 1 hit PF09346, SMI1_KNR4, 1 hit |
SMARTi | View protein in SMART SM00256, FBOX, 1 hit SM00860, SMI1_KNR4, 1 hit |
SUPFAMi | SSF110069, SSF110069, 1 hit SSF81383, SSF81383, 1 hit |
PROSITEi | View protein in PROSITE PS51087, APAG, 1 hit PS50181, FBOX, 1 hit |
ProtoNeti | Search... |
MobiDBi | Search... |
Entry informationi
Entry namei | FBX3_MOUSE | |
Accessioni | Q9DC63Primary (citable) accession number: Q9DC63 Secondary accession number(s): A2BHN5 Q9JIE4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | June 1, 2001 | |
Last modified: | December 2, 2020 | |
This is version 138 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot