UniProtKB - Q9DC33 (HM20A_MOUSE)
High mobility group protein 20A
Hmg20a
Functioni
Plays a role in neuronal differentiation as chromatin-associated protein. Acts as inhibitor of HMG20B. Overcomes the repressive effects of the neuronal silencer REST and induces the activation of neuronal-specific genes. Involved in the recruitment of the histone methyltransferase KMT2A/MLL1 and consequent increased methylation of histone H3 lysine 4.
1 PublicationRegions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
DNA bindingi | 102 – 170 | HMG boxPROSITE-ProRule annotationAdd BLAST | 69 |
GO - Molecular functioni
- DNA binding Source: UniProtKB-KW
- identical protein binding Source: MGI
GO - Biological processi
- chromatin organization Source: UniProtKB-KW
- negative regulation of neuron differentiation Source: MGI
- negative regulation of protein sumoylation Source: MGI
- negative regulation of transcription by RNA polymerase II Source: MGI
- regulation of gene expression Source: GO_Central
Keywordsi
Molecular function | Chromatin regulator, DNA-binding |
Biological process | Transcription, Transcription regulation |
Names & Taxonomyi
Protein namesi | Recommended name: High mobility group protein 20AAlternative name(s): HMG box-containing protein 20A HMG domain-containing protein HMGX1 Inhibitor of BRAF35 Short name: iBRAF |
Gene namesi | Name:Hmg20a Synonyms:Ibraf |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1914117, Hmg20a |
VEuPathDBi | HostDB:ENSMUSG00000032329 |
Subcellular locationi
Nucleus
- Nucleus PROSITE-ProRule annotation
Nucleus
- nucleus Source: MGI
Keywords - Cellular componenti
NucleusPTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000238650 | 1 – 346 | High mobility group protein 20AAdd BLAST | 346 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Modified residuei | 104 | PhosphoserineCombined sources | 1 |
Keywords - PTMi
PhosphoproteinProteomic databases
EPDi | Q9DC33 |
jPOSTi | Q9DC33 |
MaxQBi | Q9DC33 |
PaxDbi | Q9DC33 |
PeptideAtlasi | Q9DC33 |
PRIDEi | Q9DC33 |
ProteomicsDBi | 273176 [Q9DC33-1] 273177 [Q9DC33-2] 273178 [Q9DC33-3] 273179 [Q9DC33-4] |
PTM databases
iPTMneti | Q9DC33 |
PhosphoSitePlusi | Q9DC33 |
Expressioni
Tissue specificityi
Developmental stagei
Gene expression databases
Bgeei | ENSMUSG00000032329, Expressed in dorsal pancreas and 314 other tissues |
ExpressionAtlasi | Q9DC33, baseline and differential |
Genevisiblei | Q9DC33, MM |
Interactioni
Subunit structurei
Interacts with DTNB.
1 PublicationGO - Molecular functioni
- identical protein binding Source: MGI
Protein-protein interaction databases
BioGRIDi | 211774, 12 interactors |
IntActi | Q9DC33, 1 interactor |
STRINGi | 10090.ENSMUSP00000034879 |
Miscellaneous databases
RNActi | Q9DC33, protein |
Family & Domainsi
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 1 – 112 | DisorderedSequence analysisAdd BLAST | 112 | |
Regioni | 178 – 210 | DisorderedSequence analysisAdd BLAST | 33 |
Coiled coil
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Coiled coili | 228 – 272 | Sequence analysisAdd BLAST | 45 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 38 – 69 | Polar residuesSequence analysisAdd BLAST | 32 | |
Compositional biasi | 70 – 84 | Basic and acidic residuesSequence analysisAdd BLAST | 15 |
Keywords - Domaini
Coiled coilPhylogenomic databases
eggNOGi | KOG0381, Eukaryota |
GeneTreei | ENSGT00940000158464 |
HOGENOMi | CLU_060006_0_0_1 |
InParanoidi | Q9DC33 |
OMAi | MDTIDSY |
OrthoDBi | 1458939at2759 |
PhylomeDBi | Q9DC33 |
TreeFami | TF106440 |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
s (4+)i Sequence
Sequence statusi: Complete.
This entry describes 4 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 4 described isoforms and 2 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MESLMASSTL PPLFADEDGS KESNDLATSG LTHPEGPYGS AATSTTNPEF
60 70 80 90 100
VEDLSQGQLL QSEASNAVEG NEQRPEDEQR SKRGGWSKGR KRKKPLRDSN
110 120 130 140 150
APKSPLTGYV RFMNERREQL RAKRPEVPFP EITRMLGNEW SKLPPEEKQR
160 170 180 190 200
YLDEADRDKE RYMKELEQYQ KTEAYKVFSR KTQDRQKGKS HRQDAARQAT
210 220 230 240 250
HDHEKETEVK ERSVFDIPIF TEEFLNHSKA REAELRQLRK SNMEFEERNA
260 270 280 290 300
ALQKHVESMR TAVEKLEVDV IQERSRNTVL QQHLETLRQM LTSSFASMPL
310 320 330 340
PGSGEIPTVD TIDSYMNRLH SIILANPQDN ENFIATVREV VNRLDR
Computationally mapped potential isoform sequencesi
There are 2 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketA0A1L1SRA5 | A0A1L1SRA5_MOUSE | High mobility group protein 20A | Hmg20a | 180 | Annotation score: | ||
A0A1L1SSI9 | A0A1L1SSI9_MOUSE | High mobility group protein 20A | Hmg20a | 33 | Annotation score: |
Sequence cautioni
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 294 | S → C in BAC30283 (PubMed:16141072).Curated | 1 | |
Sequence conflicti | 301 | P → T in BAB30892 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_018623 | 79 – 101 | QRSKR…RDSNA → VSEHLLSLSTAASKAMGMQG GIE in isoform 4. 1 PublicationAdd BLAST | 23 | |
Alternative sequenceiVSP_018624 | 102 – 346 | Missing in isoform 4. 1 PublicationAdd BLAST | 245 | |
Alternative sequenceiVSP_018625 | 150 – 180 | RYLDE…KVFSR → VMVPASGVSLGFDYISQIVW GAGEIWGLGHA in isoform 3. 1 PublicationAdd BLAST | 31 | |
Alternative sequenceiVSP_018626 | 181 – 346 | Missing in isoform 3. 1 PublicationAdd BLAST | 166 | |
Alternative sequenceiVSP_018627 | 303 – 346 | SGEIP…NRLDR → NVILSEVSGRESGEDGDGVL FTLVLMWRSRSVSQEEKRRG KKNPVKIFSAVWACLKSQHS GGRGRKISVSSRPACPT in isoform 2. 2 PublicationsAdd BLAST | 44 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ182735 mRNA Translation: ABA26278.1 Different initiation. AK004596 mRNA Translation: BAB23397.1 AK017716 mRNA Translation: BAB30892.1 AK032790 mRNA Translation: BAC28023.1 AK039222 mRNA Translation: BAC30283.1 AK086346 mRNA Translation: BAC39652.1 AK134213 mRNA Translation: BAE22054.1 BC013804 mRNA Translation: AAH13804.1 BC068257 mRNA Translation: AAH68257.1 |
CCDSi | CCDS23208.1 [Q9DC33-1] |
RefSeqi | NP_080088.1, NM_025812.2 [Q9DC33-1] XP_006511431.1, XM_006511368.2 |
Genome annotation databases
Ensembli | ENSMUST00000034879; ENSMUSP00000034879; ENSMUSG00000032329 [Q9DC33-1] ENSMUST00000214869; ENSMUSP00000150382; ENSMUSG00000032329 [Q9DC33-4] ENSMUST00000215269; ENSMUSP00000149698; ENSMUSG00000032329 [Q9DC33-1] ENSMUST00000217518; ENSMUSP00000149359; ENSMUSG00000032329 [Q9DC33-2] |
GeneIDi | 66867 |
KEGGi | mmu:66867 |
UCSCi | uc009ptb.1, mouse [Q9DC33-4] uc009ptc.1, mouse [Q9DC33-3] uc009ptd.1, mouse [Q9DC33-2] uc009pte.1, mouse [Q9DC33-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | DQ182735 mRNA Translation: ABA26278.1 Different initiation. AK004596 mRNA Translation: BAB23397.1 AK017716 mRNA Translation: BAB30892.1 AK032790 mRNA Translation: BAC28023.1 AK039222 mRNA Translation: BAC30283.1 AK086346 mRNA Translation: BAC39652.1 AK134213 mRNA Translation: BAE22054.1 BC013804 mRNA Translation: AAH13804.1 BC068257 mRNA Translation: AAH68257.1 |
CCDSi | CCDS23208.1 [Q9DC33-1] |
RefSeqi | NP_080088.1, NM_025812.2 [Q9DC33-1] XP_006511431.1, XM_006511368.2 |
3D structure databases
AlphaFoldDBi | Q9DC33 |
SMRi | Q9DC33 |
ModBasei | Search... |
Protein-protein interaction databases
BioGRIDi | 211774, 12 interactors |
IntActi | Q9DC33, 1 interactor |
STRINGi | 10090.ENSMUSP00000034879 |
PTM databases
iPTMneti | Q9DC33 |
PhosphoSitePlusi | Q9DC33 |
Proteomic databases
EPDi | Q9DC33 |
jPOSTi | Q9DC33 |
MaxQBi | Q9DC33 |
PaxDbi | Q9DC33 |
PeptideAtlasi | Q9DC33 |
PRIDEi | Q9DC33 |
ProteomicsDBi | 273176 [Q9DC33-1] 273177 [Q9DC33-2] 273178 [Q9DC33-3] 273179 [Q9DC33-4] |
Protocols and materials databases
Antibodypediai | 1449, 319 antibodies from 33 providers |
DNASUi | 66867 |
Genome annotation databases
Ensembli | ENSMUST00000034879; ENSMUSP00000034879; ENSMUSG00000032329 [Q9DC33-1] ENSMUST00000214869; ENSMUSP00000150382; ENSMUSG00000032329 [Q9DC33-4] ENSMUST00000215269; ENSMUSP00000149698; ENSMUSG00000032329 [Q9DC33-1] ENSMUST00000217518; ENSMUSP00000149359; ENSMUSG00000032329 [Q9DC33-2] |
GeneIDi | 66867 |
KEGGi | mmu:66867 |
UCSCi | uc009ptb.1, mouse [Q9DC33-4] uc009ptc.1, mouse [Q9DC33-3] uc009ptd.1, mouse [Q9DC33-2] uc009pte.1, mouse [Q9DC33-1] |
Organism-specific databases
CTDi | 10363 |
MGIi | MGI:1914117, Hmg20a |
VEuPathDBi | HostDB:ENSMUSG00000032329 |
Phylogenomic databases
eggNOGi | KOG0381, Eukaryota |
GeneTreei | ENSGT00940000158464 |
HOGENOMi | CLU_060006_0_0_1 |
InParanoidi | Q9DC33 |
OMAi | MDTIDSY |
OrthoDBi | 1458939at2759 |
PhylomeDBi | Q9DC33 |
TreeFami | TF106440 |
Miscellaneous databases
BioGRID-ORCSi | 66867, 6 hits in 75 CRISPR screens |
ChiTaRSi | Hmg20a, mouse |
PROi | PR:Q9DC33 |
RNActi | Q9DC33, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000032329, Expressed in dorsal pancreas and 314 other tissues |
ExpressionAtlasi | Q9DC33, baseline and differential |
Genevisiblei | Q9DC33, MM |
Family and domain databases
Gene3Di | 1.10.30.10, 1 hit |
InterProi | View protein in InterPro IPR009071, HMG_box_dom IPR036910, HMG_box_dom_sf |
Pfami | View protein in Pfam PF00505, HMG_box, 1 hit |
SMARTi | View protein in SMART SM00398, HMG, 1 hit |
SUPFAMi | SSF47095, SSF47095, 1 hit |
PROSITEi | View protein in PROSITE PS50118, HMG_BOX_2, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | HM20A_MOUSE | |
Accessioni | Q9DC33Primary (citable) accession number: Q9DC33 Secondary accession number(s): Q3LSF9 Q9CYG2 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 30, 2006 |
Last sequence update: | June 1, 2001 | |
Last modified: | May 25, 2022 | |
This is version 148 of the entry and version 1 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
Reference proteomeDocuments
- MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot