UniProtKB - Q9DC28 (KC1D_MOUSE)
Casein kinase I isoform delta
Csnk1d
Functioni
Essential serine/threonine-protein kinase that regulates diverse cellular growth and survival processes including Wnt signaling, DNA repair and circadian rhythms. It can phosphorylate a large number of proteins. Casein kinases are operationally defined by their preferential utilization of acidic proteins such as caseins as substrates. Phosphorylates connexin-43/GJA1, MAP1A, SNAPIN, MAPT/TAU, TOP2A, DCK, HIF1A, EIF6, p53/TP53, DVL2, DVL3, ESR1, AIB1/NCOA3, DNMT1, PKD2, YAP1, PER1 and PER2. Central component of the circadian clock. In balance with PP1, determines the circadian period length through the regulation of the speed and rhythmicity of PER1 and PER2 phosphorylation. Controls PER1 and PER2 nuclear transport and degradation. YAP1 phosphorylation promotes its SCF(beta-TRCP) E3 ubiquitin ligase-mediated ubiquitination and subsequent degradation. DNMT1 phosphorylation reduces its DNA-binding activity. Phosphorylation of ESR1 and AIB1/NCOA3 stimulates their activity and coactivation. Phosphorylation of DVL2 and DVL3 regulates WNT3A signaling pathway that controls neurite outgrowth. EIF6 phosphorylation promotes its nuclear export. Triggers down-regulation of dopamine receptors in the forebrain. Activates DCK in vitro by phosphorylation. TOP2A phosphorylation favors DNA cleavable complex formation. May regulate the formation of the mitotic spindle apparatus in extravillous trophoblast. Modulates connexin-43/GJA1 gap junction assembly by phosphorylation. Probably involved in lymphocyte physiology. Regulates fast synaptic transmission mediated by glutamate.
9 PublicationsCatalytic activityi
- EC:2.7.11.14 PublicationsThis reaction proceeds in the forward1 Publication direction.
- EC:2.7.11.14 PublicationsThis reaction proceeds in the forward1 Publication direction.
- EC:2.7.11.26By similarityThis reaction proceeds in the forwardBy similarity direction.
- EC:2.7.11.26By similarityThis reaction proceeds in the forwardBy similarity direction.
Activity regulationi
Sites
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Binding sitei | 38 | ATPPROSITE-ProRule annotation | 1 | |
Active sitei | 128 | Proton acceptorPROSITE-ProRule annotation | 1 |
Regions
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Nucleotide bindingi | 15 – 23 | ATPPROSITE-ProRule annotation | 9 |
GO - Molecular functioni
- ATP binding Source: UniProtKB-KW
- kinase activity Source: MGI
- protein kinase activity Source: UniProtKB
- protein serine/threonine/tyrosine kinase activity Source: RHEA
- protein serine/threonine kinase activity Source: MGI
- protein serine kinase activity Source: RHEA
- tau-protein kinase activity Source: UniProtKB-EC
GO - Biological processi
- circadian regulation of gene expression Source: UniProtKB
- Golgi organization Source: MGI
- microtubule nucleation Source: MGI
- non-motile cilium assembly Source: MGI
- peptidyl-serine phosphorylation Source: MGI
- positive regulation of canonical Wnt signaling pathway Source: MGI
- positive regulation of non-canonical Wnt signaling pathway Source: ParkinsonsUK-UCL
- positive regulation of proteasomal ubiquitin-dependent protein catabolic process Source: UniProtKB
- positive regulation of protein phosphorylation Source: MGI
- positive regulation of Wnt-mediated midbrain dopaminergic neuron differentiation Source: ParkinsonsUK-UCL
- positive regulation of Wnt signaling pathway Source: ParkinsonsUK-UCL
- protein localization to centrosome Source: MGI
- protein localization to cilium Source: MGI
- protein localization to Golgi apparatus Source: MGI
- protein phosphorylation Source: UniProtKB
- regulation of circadian rhythm Source: UniProtKB
- signal transduction Source: GO_Central
- spindle assembly Source: MGI
- Wnt signaling pathway Source: UniProtKB-KW
Keywordsi
Molecular function | Kinase, Serine/threonine-protein kinase, Transferase |
Biological process | Biological rhythms, Wnt signaling pathway |
Ligand | ATP-binding, Nucleotide-binding |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-8854518, AURKA Activation by TPX2 |
Names & Taxonomyi
Protein namesi | |
Gene namesi | Name:Csnk1d Synonyms:Hckid |
Organismi | Mus musculus (Mouse) |
Taxonomic identifieri | 10090 [NCBI] |
Taxonomic lineagei | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Myomorpha › Muroidea › Muridae › Murinae › Mus › Mus |
Proteomesi |
|
Organism-specific databases
MGIi | MGI:1355272, Csnk1d |
VEuPathDBi | HostDB:ENSMUSG00000025162 |
Subcellular locationi
Golgi apparatus
- Golgi apparatus By similarity
Nucleus
- Nucleus 3 Publications
Plasma membrane
- Cell membrane By similarity
Cytoskeleton
- centrosome By similarity
- spindle By similarity
Cytoplasm and Cytosol
- Cytoplasm By similarity
Other locations
- perinuclear region By similarity
Note: Localized at mitotic spindle microtubules, and at the centrosomes and interphase in interphase cells. Recruited to the spindle apparatus and the centrosomes in response to DNA-damage. Correct subcellular localization requires kinase activity (By similarity).By similarity
Cytoskeleton
- centrosome Source: MGI
- ciliary basal body Source: MGI
- spindle Source: MGI
- spindle microtubule Source: MGI
Cytosol
- cytosol Source: Reactome
Golgi apparatus
- Golgi apparatus Source: MGI
Nucleus
- nucleoplasm Source: Reactome
- nucleus Source: UniProtKB
Plasma Membrane
- plasma membrane Source: MGI
Other locations
- cytoplasm Source: GO_Central
- neuron projection Source: MGI
- perinuclear region of cytoplasm Source: MGI
Keywords - Cellular componenti
Cell membrane, Cytoplasm, Cytoskeleton, Golgi apparatus, Membrane, NucleusPathology & Biotechi
Disruption phenotypei
Mutagenesis
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Mutagenesisi | 44 | T → A: Increases pain sensitivity; reduces threshold for induction of cortical spreading depression; increases arterial dilation during cortical spreading depression and increases spontaneous and evoked calcium signaling in astrocytes. 1 Publication | 1 |
Chemistry databases
ChEMBLi | CHEMBL5175 |
PTM / Processingi
Molecule processing
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
ChainiPRO_0000192834 | 1 – 415 | Casein kinase I isoform deltaAdd BLAST | 415 |
Amino acid modifications
Feature key | Position(s) | DescriptionActions | Graphical view | Length | |
---|---|---|---|---|---|
Modified residuei | 328 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 331 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 370 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 375 | Omega-N-methylarginineCombined sources | 1 | ||
Modified residuei | 382 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 383 | PhosphoserineCombined sources | 1 | ||
Modified residuei | 384 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 407 | PhosphoserineBy similarity | 1 | ||
Modified residuei | 411 | PhosphoserineBy similarity | 1 | ||
Isoform 2 (identifier: Q9DC28-2) | |||||
Modified residuei | 401 | PhosphoserineBy similarity | 1 |
Post-translational modificationi
Keywords - PTMi
Methylation, PhosphoproteinProteomic databases
EPDi | Q9DC28 |
jPOSTi | Q9DC28 |
MaxQBi | Q9DC28 |
PaxDbi | Q9DC28 |
PeptideAtlasi | Q9DC28 |
PRIDEi | Q9DC28 |
ProteomicsDBi | 263482 [Q9DC28-1] 263483 [Q9DC28-2] |
PTM databases
iPTMneti | Q9DC28 |
PhosphoSitePlusi | Q9DC28 |
SwissPalmi | Q9DC28 |
Expressioni
Tissue specificityi
Gene expression databases
Bgeei | ENSMUSG00000025162, Expressed in paneth cell and 330 other tissues |
ExpressionAtlasi | Q9DC28, baseline and differential |
Genevisiblei | Q9DC28, MM |
Interactioni
Subunit structurei
Monomer (By similarity).
Component of the circadian core oscillator, which includes the CRY proteins, CLOCK, or NPAS2, ARNTL/BMAL1 or ARNTL2/BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins (PubMed:11779462).
Interacts directly with PER1 and PER2 which may lead to their degradation (By similarity).
Interacts with MAP1A (By similarity).
Interacts with MAPT/TAU, DBNDD2, AIB1/NCOA3 and ESR1 (By similarity).
Interacts with AKAP9/AKAP450; this interaction promotes centrosomal subcellular location (By similarity). Binds to tubulins in mitotic cells upon DNA damage (By similarity).
Interacts with GJA1 (By similarity).
Interacts with SNAPIN (PubMed:17101137).
Interacts with DNMT1 (PubMed:20192920).
Interacts with DDX3X; this interaction enhances CSNK1D kinase activity in vitro, but it is unclear whether this interaction is physiologically relevant (By similarity).
By similarity3 PublicationsProtein-protein interaction databases
BioGRIDi | 222545, 24 interactors |
DIPi | DIP-47809N |
IntActi | Q9DC28, 21 interactors |
MINTi | Q9DC28 |
STRINGi | 10090.ENSMUSP00000018274 |
Miscellaneous databases
RNActi | Q9DC28, protein |
Structurei
Secondary structure
3D structure databases
AlphaFoldDBi | Q9DC28 |
SMRi | Q9DC28 |
ModBasei | Search... |
PDBe-KBi | Search... |
Family & Domainsi
Domains and Repeats
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Domaini | 9 – 277 | Protein kinasePROSITE-ProRule annotationAdd BLAST | 269 |
Region
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Regioni | 278 – 364 | Centrosomal localization signal (CLS)By similarityAdd BLAST | 87 | |
Regioni | 301 – 415 | DisorderedSequence analysisAdd BLAST | 115 | |
Regioni | 317 – 342 | AutoinhibitoryBy similarityAdd BLAST | 26 |
Compositional bias
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Compositional biasi | 301 – 320 | Basic and acidic residuesSequence analysisAdd BLAST | 20 | |
Compositional biasi | 342 – 359 | Polar residuesSequence analysisAdd BLAST | 18 | |
Compositional biasi | 380 – 415 | Polar residuesSequence analysisAdd BLAST | 36 |
Sequence similaritiesi
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000153536 |
HOGENOMi | CLU_019279_2_2_1 |
InParanoidi | Q9DC28 |
OMAi | IFDWTFL |
PhylomeDBi | Q9DC28 |
TreeFami | TF300544 |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
s (2+)i Sequence
Sequence statusi: Complete.
This entry describes 2 produced by isoformsialternative splicing. AlignAdd to basketThis entry has 2 described isoforms and 3 potential isoforms that are computationally mapped.Show allAlign All
This isoform has been chosen as the sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry. canonicali
10 20 30 40 50
MELRVGNRYR LGRKIGSGSF GDIYLGTDIA AGEEVAIKLE CVKTKHPQLH
60 70 80 90 100
IESKIYKMMQ GGVGIPTIRW CGAEGDYNVM VMELLGPSLE DLFNFCSRKF
110 120 130 140 150
SLKTVLLLAD QMISRIEYIH SKNFIHRDVK PDNFLMGLGK KGNLVYIIDF
160 170 180 190 200
GLAKKYRDAR THQHIPYREN KNLTGTARYA SINTHLGIEQ SRRDDLESLG
210 220 230 240 250
YVLMYFNLGS LPWQGLKAAT KRQKYERISE KKMSTPIEVL CKGYPSEFAT
260 270 280 290 300
YLNFCRSLRF DDKPDYSYLR QLFRNLFHRQ GFSYDYVFDW NMLKFGASRA
310 320 330 340 350
ADDAERERRD REERLRHSRN PATRGLPSTA SGRLRGTQEV APPTPLTPTS
360 370 380 390 400
HTANTSPRPV SGMERERKVS MRLHRGAPVN VSSSDLTGRQ DTSRMSTSQI
410
PGRVASSGLQ SVVHR
Computationally mapped potential isoform sequencesi
There are 3 potential isoforms mapped to this entry.BLASTAlignShow allAdd to basketQ3USK2 | Q3USK2_MOUSE | Casein kinase I isoform delta | Csnk1d | 428 | Annotation score: | ||
F7A4U8 | F7A4U8_MOUSE | Casein kinase I isoform delta | Csnk1d | 218 | Annotation score: | ||
F6VU53 | F6VU53_MOUSE | Casein kinase I isoform delta | Csnk1d | 68 | Annotation score: |
Experimental Info
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Sequence conflicti | 313 | E → G in BAB23405 (PubMed:16141072).Curated | 1 |
Alternative sequence
Feature key | Position(s) | DescriptionActions | Graphical view | Length |
---|---|---|---|---|
Alternative sequenceiVSP_010254 | 400 – 415 | IPGRV…SVVHR → NSIPFEHHGK in isoform 2. 1 PublicationAdd BLAST | 16 |
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004606 mRNA Translation: BAB23405.1 AK088642 mRNA Translation: BAC40472.1 AK152721 mRNA Translation: BAE31444.1 AK157812 mRNA Translation: BAE34206.1 BC004604 mRNA Translation: AAH04604.1 |
CCDSi | CCDS25762.1 [Q9DC28-1] CCDS25763.1 [Q9DC28-2] |
PIRi | S47616 |
RefSeqi | NP_620690.1, NM_139059.2 [Q9DC28-1] |
Genome annotation databases
Ensembli | ENSMUST00000018274; ENSMUSP00000018274; ENSMUSG00000025162 [Q9DC28-1] ENSMUST00000070575; ENSMUSP00000070721; ENSMUSG00000025162 [Q9DC28-2] |
GeneIDi | 104318 |
KEGGi | mmu:104318 |
UCSCi | uc007mvb.1, mouse [Q9DC28-1] |
Keywords - Coding sequence diversityi
Alternative splicingSimilar proteinsi
Cross-referencesi
Sequence databases
Select the link destinations: EMBLi GenBanki DDBJi Links Updated | AK004606 mRNA Translation: BAB23405.1 AK088642 mRNA Translation: BAC40472.1 AK152721 mRNA Translation: BAE31444.1 AK157812 mRNA Translation: BAE34206.1 BC004604 mRNA Translation: AAH04604.1 |
CCDSi | CCDS25762.1 [Q9DC28-1] CCDS25763.1 [Q9DC28-2] |
PIRi | S47616 |
RefSeqi | NP_620690.1, NM_139059.2 [Q9DC28-1] |
3D structure databases
Select the link destinations: PDBei RCSB PDBi PDBji Links Updated | PDB entry | Method | Resolution (Å) | Chain | Positions | PDBsum |
4JJR | X-ray | 2.41 | A/B | 1-299 | [»] | |
5X17 | X-ray | 2.00 | A/B | 1-294 | [»] | |
AlphaFoldDBi | Q9DC28 | |||||
SMRi | Q9DC28 | |||||
ModBasei | Search... | |||||
PDBe-KBi | Search... |
Protein-protein interaction databases
BioGRIDi | 222545, 24 interactors |
DIPi | DIP-47809N |
IntActi | Q9DC28, 21 interactors |
MINTi | Q9DC28 |
STRINGi | 10090.ENSMUSP00000018274 |
Chemistry databases
ChEMBLi | CHEMBL5175 |
PTM databases
iPTMneti | Q9DC28 |
PhosphoSitePlusi | Q9DC28 |
SwissPalmi | Q9DC28 |
Proteomic databases
EPDi | Q9DC28 |
jPOSTi | Q9DC28 |
MaxQBi | Q9DC28 |
PaxDbi | Q9DC28 |
PeptideAtlasi | Q9DC28 |
PRIDEi | Q9DC28 |
ProteomicsDBi | 263482 [Q9DC28-1] 263483 [Q9DC28-2] |
Protocols and materials databases
Antibodypediai | 4210, 371 antibodies from 40 providers |
DNASUi | 104318 |
Genome annotation databases
Ensembli | ENSMUST00000018274; ENSMUSP00000018274; ENSMUSG00000025162 [Q9DC28-1] ENSMUST00000070575; ENSMUSP00000070721; ENSMUSG00000025162 [Q9DC28-2] |
GeneIDi | 104318 |
KEGGi | mmu:104318 |
UCSCi | uc007mvb.1, mouse [Q9DC28-1] |
Organism-specific databases
CTDi | 1453 |
MGIi | MGI:1355272, Csnk1d |
VEuPathDBi | HostDB:ENSMUSG00000025162 |
Phylogenomic databases
eggNOGi | KOG1164, Eukaryota |
GeneTreei | ENSGT00940000153536 |
HOGENOMi | CLU_019279_2_2_1 |
InParanoidi | Q9DC28 |
OMAi | IFDWTFL |
PhylomeDBi | Q9DC28 |
TreeFami | TF300544 |
Enzyme and pathway databases
BRENDAi | 2.7.11.1, 3474 |
Reactomei | R-MMU-204005, COPII-mediated vesicle transport R-MMU-2565942, Regulation of PLK1 Activity at G2/M Transition R-MMU-380259, Loss of Nlp from mitotic centrosomes R-MMU-380270, Recruitment of mitotic centrosome proteins and complexes R-MMU-380284, Loss of proteins required for interphase microtubule organization from the centrosome R-MMU-380320, Recruitment of NuMA to mitotic centrosomes R-MMU-5620912, Anchoring of the basal body to the plasma membrane R-MMU-6791226, Major pathway of rRNA processing in the nucleolus and cytosol R-MMU-8854518, AURKA Activation by TPX2 |
Miscellaneous databases
BioGRID-ORCSi | 104318, 3 hits in 77 CRISPR screens |
ChiTaRSi | Csnk1d, mouse |
PROi | PR:Q9DC28 |
RNActi | Q9DC28, protein |
SOURCEi | Search... |
Gene expression databases
Bgeei | ENSMUSG00000025162, Expressed in paneth cell and 330 other tissues |
ExpressionAtlasi | Q9DC28, baseline and differential |
Genevisiblei | Q9DC28, MM |
Family and domain databases
InterProi | View protein in InterPro IPR011009, Kinase-like_dom_sf IPR000719, Prot_kinase_dom IPR017441, Protein_kinase_ATP_BS IPR008271, Ser/Thr_kinase_AS |
Pfami | View protein in Pfam PF00069, Pkinase, 1 hit |
SMARTi | View protein in SMART SM00220, S_TKc, 1 hit |
SUPFAMi | SSF56112, SSF56112, 1 hit |
PROSITEi | View protein in PROSITE PS00107, PROTEIN_KINASE_ATP, 1 hit PS50011, PROTEIN_KINASE_DOM, 1 hit PS00108, PROTEIN_KINASE_ST, 1 hit |
MobiDBi | Search... |
Entry informationi
Entry namei | KC1D_MOUSE | |
Accessioni | Q9DC28Primary (citable) accession number: Q9DC28 Secondary accession number(s): Q3TZK2, Q99KK4 | |
Entry historyi | Integrated into UniProtKB/Swiss-Prot: | May 10, 2004 |
Last sequence update: | May 10, 2004 | |
Last modified: | May 25, 2022 | |
This is version 168 of the entry and version 2 of the sequence. See complete history. | ||
Entry statusi | Reviewed (UniProtKB/Swiss-Prot) | |
Annotation program | Chordata Protein Annotation Program |
Miscellaneousi
Keywords - Technical termi
3D-structure, Reference proteomeDocuments
- Human and mouse protein kinases
Human and mouse protein kinases: classification and index - MGD cross-references
Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot - PDB cross-references
Index of Protein Data Bank (PDB) cross-references - SIMILARITY comments
Index of protein domains and families