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Protein

Eukaryotic translation initiation factor 3 subunit K

Gene

Eif3k

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is required for several steps in the initiation of protein synthesis. The eIF-3 complex associates with the 40S ribosome and facilitates the recruitment of eIF-1, eIF-1A, eIF-2:GTP:methionyl-tRNAi and eIF-5 to form the 43S pre-initiation complex (43S PIC). The eIF-3 complex stimulates mRNA recruitment to the 43S PIC and scanning of the mRNA for AUG recognition. The eIF-3 complex is also required for disassembly and recycling of post-termination ribosomal complexes and subsequently prevents premature joining of the 40S and 60S ribosomal subunits prior to initiation. The eIF-3 complex specifically targets and initiates translation of a subset of mRNAs involved in cell proliferation, including cell cycling, differentiation and apoptosis, and uses different modes of RNA stem-loop binding to exert either translational activation or repression.UniRule annotation1 Publication

GO - Molecular functioni

GO - Biological processi

  • regulation of translational initiation Source: InterPro
  • translational initiation Source: UniProtKB

Keywordsi

Molecular functionInitiation factor
Biological processProtein biosynthesis

Enzyme and pathway databases

ReactomeiR-MMU-156827 L13a-mediated translational silencing of Ceruloplasmin expression
R-MMU-72649 Translation initiation complex formation
R-MMU-72689 Formation of a pool of free 40S subunits
R-MMU-72695 Formation of the ternary complex, and subsequently, the 43S complex
R-MMU-72702 Ribosomal scanning and start codon recognition

Names & Taxonomyi

Protein namesi
Recommended name:
Eukaryotic translation initiation factor 3 subunit KUniRule annotation
Short name:
eIF3kUniRule annotation
Alternative name(s):
Eukaryotic translation initiation factor 3 subunit 12UniRule annotation
eIF-3 p25UniRule annotation
Gene namesi
Name:Eif3k
Synonyms:Eif3s12
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 7

Organism-specific databases

MGIiMGI:1921080 Eif3k

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedUniRule annotation
ChainiPRO_00001235472 – 218Eukaryotic translation initiation factor 3 subunit KAdd BLAST217

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineUniRule annotationBy similarity1
Modified residuei28PhosphothreonineBy similarity1
Modified residuei217PhosphoserineBy similarity1

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiQ9DBZ5
PaxDbiQ9DBZ5
PeptideAtlasiQ9DBZ5
PRIDEiQ9DBZ5
TopDownProteomicsiQ9DBZ5-1 [Q9DBZ5-1]

PTM databases

iPTMnetiQ9DBZ5
PhosphoSitePlusiQ9DBZ5
SwissPalmiQ9DBZ5

Expressioni

Gene expression databases

BgeeiENSMUSG00000053565
ExpressionAtlasiQ9DBZ5 baseline and differential
GenevisibleiQ9DBZ5 MM

Interactioni

Subunit structurei

Component of the eukaryotic translation initiation factor 3 (eIF-3) complex, which is composed of 13 subunits: EIF3A, EIF3B, EIF3C, EIF3D, EIF3E, EIF3F, EIF3G, EIF3H, EIF3I, EIF3J, EIF3K, EIF3L and EIF3M. The eIF-3 complex appears to include 3 stable modules: module A is composed of EIF3A, EIF3B, EIF3G and EIF3I; module B is composed of EIF3F, EIF3H, and EIF3M; and module C is composed of EIF3C, EIF3D, EIF3E, EIF3K and EIF3L. EIF3C of module C binds EIF3B of module A and EIF3H of module B, thereby linking the three modules. EIF3J is a labile subunit that binds to the eIF-3 complex via EIF3B. The eIF-3 complex interacts with RPS6KB1 under conditions of nutrient depletion. Mitogenic stimulation leads to binding and activation of a complex composed of MTOR and RPTOR, leading to phosphorylation and release of RPS6KB1 and binding of EIF4B to eIF-3. Identified in a HCV IRES-mediated translation complex, at least composed of EIF3C, IGF2BP1, RPS3 and HCV RNA-replicon. Interacts with ALKBH4, IFIT1 and IFIT2.UniRule annotation1 Publication

Protein-protein interaction databases

BioGridi216289, 3 interactors
IntActiQ9DBZ5, 3 interactors
MINTiQ9DBZ5
STRINGi10090.ENSMUSP00000066038

Structurei

3D structure databases

ProteinModelPortaliQ9DBZ5
SMRiQ9DBZ5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini42 – 204PCIPROSITE-ProRule annotationAdd BLAST163

Sequence similaritiesi

Belongs to the eIF-3 subunit K family.UniRule annotation

Phylogenomic databases

eggNOGiKOG3252 Eukaryota
ENOG410ZSE4 LUCA
GeneTreeiENSGT00390000009409
HOGENOMiHOG000008222
HOVERGENiHBG052081
InParanoidiQ9DBZ5
KOiK15028
OMAiYVQDQAK
OrthoDBiEOG091G0IXT
PhylomeDBiQ9DBZ5
TreeFamiTF314893

Family and domain databases

Gene3Di1.10.10.10, 1 hit
1.25.40.250, 1 hit
HAMAPiMF_03010 eIF3k, 1 hit
InterProiView protein in InterPro
IPR016024 ARM-type_fold
IPR033464 CSN8_PSD8_EIF3K
IPR009374 eIF3k
IPR000717 PCI_dom
IPR016020 Transl_init_fac_sub12_N_euk
IPR036388 WH-like_DNA-bd_sf
IPR036390 WH_DNA-bd_sf
PANTHERiPTHR13022 PTHR13022, 1 hit
PfamiView protein in Pfam
PF10075 CSN8_PSD8_EIF3K, 1 hit
SUPFAMiSSF46785 SSF46785, 1 hit
SSF48371 SSF48371, 1 hit
PROSITEiView protein in PROSITE
PS50250 PCI, 1 hit

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: Q9DBZ5-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAMFEQMRAN VGKLLKGIDR YNPENLATLE RYVETQAKEN AYDLEANLAV
60 70 80 90 100
LKLYQFNPAF FQTTVTAQIL LKALTNLPHT DFTLCKCMID QAHQEERPIR
110 120 130 140 150
QILYLGDLLE TCHFQAFWQA LDENMDLLEG ITGFEDSVRK FICHVVGITY
160 170 180 190 200
QHIDRWLLAE MLGDLTDNQL KVWMSKYGWS ADESGQVFIC SQEESIKPKN
210
IVEKIDFDSV SSIMASSQ
Length:218
Mass (Da):25,087
Last modified:June 1, 2001 - v1
Checksum:i84173F6E77F8C294
GO
Isoform 2 (identifier: Q9DBZ5-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     21-53: Missing.

Note: No experimental confirmation available.
Show »
Length:185
Mass (Da):21,307
Checksum:i509E5FB991EAAEB9
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_01342521 – 53Missing in isoform 2. 1 PublicationAdd BLAST33

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK004664 mRNA Translation: BAB23454.1
BC027638 mRNA Translation: AAH27638.1
BC091749 mRNA Translation: AAH91749.1
CCDSiCCDS21062.1 [Q9DBZ5-1]
CCDS85254.1 [Q9DBZ5-2]
RefSeqiNP_001272871.1, NM_001285942.1 [Q9DBZ5-2]
NP_082935.1, NM_028659.3 [Q9DBZ5-1]
UniGeneiMm.29714

Genome annotation databases

EnsembliENSMUST00000066070; ENSMUSP00000066038; ENSMUSG00000053565 [Q9DBZ5-1]
ENSMUST00000207683; ENSMUSP00000146940; ENSMUSG00000053565 [Q9DBZ5-2]
GeneIDi73830
KEGGimmu:73830
UCSCiuc009gai.2 mouse [Q9DBZ5-1]
uc009gaj.2 mouse [Q9DBZ5-2]

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiEIF3K_MOUSE
AccessioniPrimary (citable) accession number: Q9DBZ5
Secondary accession number(s): Q58EU9, Q8K3A1
Entry historyiIntegrated into UniProtKB/Swiss-Prot: September 19, 2002
Last sequence update: June 1, 2001
Last modified: July 18, 2018
This is version 128 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Translation initiation factors
    List of translation initiation factor entries
  2. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

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